Current Search: FAU Undergraduate Student Research (x) » Esiobu, Nwadiuto (x)
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Title
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A simple method for measuring antibiotic resistance in a community of bacteria.
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Creator
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Pelski, Ana, Samaroo, Sheynelle, Varughese, Vipin, Behr, Evalena, Browne, Trisha, Esiobu, Nwadiuto
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Abstract/Description
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Antibiotic resistant bacteria infiltrate drinking water, increase human exposure, and spread into clinical settings. Regulatory agencies need simple test for ARI to monitor environmental quality. This study is first to measure resistance in a consortium of bacteria, not pure cultures. Our rapid method – “In-the-zone” (ITZ) employs a) the size of zones of inhibition b) identity of bacteria in the zone c) number of resistant clones within zones of inhibition and d) type of antibiotic to compute...
Show moreAntibiotic resistant bacteria infiltrate drinking water, increase human exposure, and spread into clinical settings. Regulatory agencies need simple test for ARI to monitor environmental quality. This study is first to measure resistance in a consortium of bacteria, not pure cultures. Our rapid method – “In-the-zone” (ITZ) employs a) the size of zones of inhibition b) identity of bacteria in the zone c) number of resistant clones within zones of inhibition and d) type of antibiotic to compute ARI. Results can be obtained within 24 - 48 hrs with very little effort. This semester, we repeated the test and validated the weighting of various parameters. The ITZ assay yield consistent and reproducible data on zones of inhibition of bacteria community. The diameter of inhibition zones was the most important parameter distinguishing resistomes of varying ARI. Landfill, manured garden, and waste plant samples scored highest in antibiotic resistance index compared to beach sand.
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Date Issued
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2014
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PURL
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http://purl.flvc.org/fau/fd/FA0005034
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Subject Headings
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College students --Research --United States.
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Format
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Document (PDF)
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Title
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Microbial Community Resiliency and Role in Sea Level Rise.
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Creator
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Klein, Christian, Esiobu, Nwadiuto, Office of Undergraduate Research and Inquiry
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Abstract/Description
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The interphase (littoral zone) of the Florida Everglades and surrounding marine environments are dominated by mangrove forests and mudflats that transition into freshwater communities. As salinity levels change, plant cover of the freshwater community will be adversely affected, but perhaps more importantly, their microbial symbionts responsible for their tolerance to high salinity will be impacted as well. Microbes respond quicker to changing environments and could provide an early warning...
Show moreThe interphase (littoral zone) of the Florida Everglades and surrounding marine environments are dominated by mangrove forests and mudflats that transition into freshwater communities. As salinity levels change, plant cover of the freshwater community will be adversely affected, but perhaps more importantly, their microbial symbionts responsible for their tolerance to high salinity will be impacted as well. Microbes respond quicker to changing environments and could provide an early warning of the loss of resiliency of the Florida coastline ecosystem. For 10 weeks, soil samples from Biscayne National Park will be placed in standardized mesocosms and subject to local conditions like sunlight, tides, plants, increased sea levels, higher salinities, and variant inundation. Standard and variant conditions will be employed on different mesocosms, and weekly DNA extractions of the various soil samples will be analyzed via metagenomic analysis, creating a library of the 16S rRNA community structure over the 10 week period.
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Date Issued
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2017
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PURL
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http://purl.flvc.org/fau/fd/FA00005623
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Subject Headings
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College students --Research --United States.
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Format
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Document (PDF)
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Title
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In search of environmental hotspots of antibiotic resistance.
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Creator
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Desir, Hedson, Esiobu, Nwadiuto
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Date Issued
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2013-04-05
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PURL
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http://purl.flvc.org/fcla/dt/3361076
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Subject Headings
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Antibiotics resistance in microorganisms, Environmental hotspots
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Format
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Document (PDF)