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- Title
- Characterization, biosynthetic studies and subcellular localization of sterols from the marine sponge Teichaxinella morchella.
- Creator
- Williams, Amanda Joy, Florida Atlantic University, Kerr, Russell G.
- Abstract/Description
-
A-nor sterols have been isolated and characterized from the marine sponge Teichaxinella morchella using High Performance Liquid Chromatography, Nuclear Magnetic Resonance, and Mass Spectrometry. A cell free extract of T. morchella was used to determine the biosynthetic origin of the sterols. Incubation experiments with radiolabled Farnesyl Pyrophosphate and cholesterol showed that the sterols are coming from a dietary source. Subcellular localization studies showed that the sterols are part...
Show moreA-nor sterols have been isolated and characterized from the marine sponge Teichaxinella morchella using High Performance Liquid Chromatography, Nuclear Magnetic Resonance, and Mass Spectrometry. A cell free extract of T. morchella was used to determine the biosynthetic origin of the sterols. Incubation experiments with radiolabled Farnesyl Pyrophosphate and cholesterol showed that the sterols are coming from a dietary source. Subcellular localization studies showed that the sterols are part of the cell membrane.
Show less - Date Issued
- 2001
- PURL
- http://purl.flvc.org/fcla/dt/12865
- Subject Headings
- Sponges, Sterols
- Format
- Document (PDF)
- Title
- ESTABLISHING A SPONGE HYBRIDOMA FOR THE IN VITRO PRODUCTION OF A MARINE NATURAL PRODUCT.
- Creator
- Dougan, Cassady, Pomponi, Shirley A., Florida Atlantic University, Department of Marine Science and Oceanography, Charles E. Schmidt College of Science
- Abstract/Description
-
Marine sponges are one of the most prolific sources of chemical compounds with pharmaceutical importance. To establish a supply of such compounds large enough for clinical development, in vitro production methodology was investigated. Since all sponge cells do not divide in culture, it was hypothesized that the fusion of rapidly dividing cells of a sponge that does not produce any compounds of interest with cells of a nondividing but compound-producing sponge would result in a hybridoma that...
Show moreMarine sponges are one of the most prolific sources of chemical compounds with pharmaceutical importance. To establish a supply of such compounds large enough for clinical development, in vitro production methodology was investigated. Since all sponge cells do not divide in culture, it was hypothesized that the fusion of rapidly dividing cells of a sponge that does not produce any compounds of interest with cells of a nondividing but compound-producing sponge would result in a hybridoma that produces the compound of interest. In this study, hybridomas have been produced with cells of two marine sponges, Axinella corrugata, which produces the antitumor compound stevensine, and Geodia neptuni, which divides rapidly in a nutrient medium optimized for sponge cell culture. Successful hybridization and subsequent cell division and in vitro stevensine production may unlock the potential for sustainable mass production of other sponge-derived compounds.
Show less - Date Issued
- 2022
- PURL
- http://purl.flvc.org/fau/fd/FA00014024
- Subject Headings
- Marine natural products, Sponges, Hybridomas, In vitro
- Format
- Document (PDF)
- Title
- EVALUATION OF 3-D CULTURE METHODS FOR MARINE SPONGES.
- Creator
- Urban-Gedamke, Elizabeth, Pomponi, Shirley A., Florida Atlantic University, Department of Marine Science and Oceanography, Charles E. Schmidt College of Science
- Abstract/Description
-
Marine sponges are economically and environmentally valuable, but restoration, commercial, and biomedical demands exceed what wild populations and aquaculture can provide. In vitro culture of sponge cells is a promising alternative, but has remained elusive until recent breakthroughs involving improved nutrient medium M1 in two-dimensional culture. The advantages of three-dimensional over two-dimensional cell culture have been increasingly recognized. Here we report the successful 21-day...
Show moreMarine sponges are economically and environmentally valuable, but restoration, commercial, and biomedical demands exceed what wild populations and aquaculture can provide. In vitro culture of sponge cells is a promising alternative, but has remained elusive until recent breakthroughs involving improved nutrient medium M1 in two-dimensional culture. The advantages of three-dimensional over two-dimensional cell culture have been increasingly recognized. Here we report the successful 21-day culture of cells from the marine sponge Geodia neptuni using multiple three-dimensional cell culture methods: FibraCel© disks, thin hydrogel layers, gel micro droplets, and spheroid cell culture. These methods performed comparably to two-dimensional control cultures, and each method offers advantages for restoration or in vitro applications using sponge cells. Further optimization of these methods may lead to the ability to culture fully functioning sponges from dissociated, cryopreserved cells, which will reduce the need for wild harvest of sponge tissues for commercial, restoration, and biomedical purposes.
Show less - Date Issued
- 2021
- PURL
- http://purl.flvc.org/fau/fd/FA00013726
- Subject Headings
- Sponges, Cell Culture--methods
- Format
- Document (PDF)
- Title
- Strategies for flow cytometric analysis of marine microalgae and sponge cells.
- Creator
- Yentsch, C. M., Pomponi, Shirley A.
- Date Issued
- 1994
- PURL
- http://purl.flvc.org/FCLA/DT/3353886
- Subject Headings
- Microalgae, Sponges, Cytometric cellular analysis
- Format
- Document (PDF)
- Title
- Batzellines A, B, and C. Novel pyrroloquinoline alkaloids from the sponge Batzella sp.
- Creator
- Sakemi, S., Sun, H. H., Jefford, C. W., Bernardinelli, G., Harbor Branch Oceanographic Institute
- Date Issued
- 1989
- PURL
- http://purl.flvc.org/FCLA/DT/3353844
- Subject Headings
- Sponges, Alkaloids, Pyrroles, Quinolines, Chemical structure
- Format
- Document (PDF)
- Title
- New cytotoxic acridine alkaloids from two deep water marine sponges of the family Pachastrellidae.
- Creator
- Gunawardana, G. P., Kohmoto, S., Burres, N. S., Harbor Branch Oceanographic Institute
- Date Issued
- 1989
- PURL
- http://purl.flvc.org/FCLA/DT/3353842
- Subject Headings
- Sponges, Acridines, Alkaloids, Cytotoxins
- Format
- Document (PDF)
- Title
- New manzamine alkaloids from a sponge of the genus xestospongia.
- Creator
- Ichiba, T., Sakai, R., Kohmoto, S., Saucy, G., Higa, T., Harbor Branch Oceanographic Institute
- Date Issued
- 1988
- PURL
- http://purl.flvc.org/FCLA/DT/3353822
- Subject Headings
- Alkaloids, Indole Alkaloids, Sponges, Cytotoxin, Carbazoles, Chemical structure
- Format
- Document (PDF)
- Title
- Antitumor macrodiolides isolated from a marine sponge Theonella sp.: structure revision of misakinolide A.
- Creator
- Kato, Y., Fusetani, N., Matsunaga, S., Hashimoto, K., Sakai, R., Higa, T., Kashman, Y., Harbor Branch Oceanographic Institute
- Date Issued
- 1987
- PURL
- http://purl.flvc.org/FCLA/DT/3353820
- Subject Headings
- Sponges, Antineoplastic agents, Macrolides, Chemical structure, Marine natural products
- Format
- Document (PDF)
- Title
- Manzamine B and C, two novel alkaloids from the sponge Haliciona sp.
- Creator
- Sakai, R., Kohmoto, S., Higa, T., Jefford, C. W., Bernardinelli, G., Harbor Branch Oceanographic Institute
- Date Issued
- 1987
- PURL
- http://purl.flvc.org/FCLA/DT/3353812
- Subject Headings
- Indole Alkaloids, Sponges, Carbolines
- Format
- Document (PDF)
- Title
- Reiswigins A and B, novel antiviral diterpenes from a deepwater sponge.
- Creator
- Kashman, Y., Hirsch, S., Koehn, F. E., Cross, S. S., Harbor Branch Oceanographic Institute
- Date Issued
- 1987
- PURL
- http://purl.flvc.org/FCLA/DT/3353808
- Subject Headings
- Antiviral agents, Diterpenes, Sponges, Marine metabolites
- Format
- Document (PDF)
- Title
- Antitumor cyclic peroxides from the sponge Plakortis lita.
- Creator
- Sakemi, S., Higa, T., Anthoni, U., Christophersen, C., Harbor Branch Oceanographic Institute
- Date Issued
- 1987
- PURL
- http://purl.flvc.org/FCLA/DT/3353800
- Subject Headings
- Antineoplastic agents, Sponges, Peroxides, Marine metabolites
- Format
- Document (PDF)
- Title
- Duryne, a new cytotoxic agent from the marine sponge Cribrochalina dura.
- Creator
- Wright, Amy E., McConnell, O. J., Kohmoto, S., Lui, M. S., Thompson, W., Snader, K. M.
- Date Issued
- 1987
- PURL
- http://purl.flvc.org/FCLA/DT/3353798
- Subject Headings
- Cytotoxin, Sponges, Marine natural products, Polyacetylenes
- Format
- Document (PDF)
- Title
- Farming sponges for chemicals with pharmaceutical potential.
- Creator
- Duckworth, Alan R., Harbor Branch Oceanographic Institute
- Date Issued
- 2001
- PURL
- http://purl.flvc.org/FCLA/DT/3352965
- Subject Headings
- Sponges, Metabolites, Sponges--Biotechnology, Marine metabolites, Marine pharmacology
- Format
- Document (PDF)
- Title
- Chemistry and biology of the discodermolides, potent mitotic spindle poisons.
- Creator
- Gunasekera, Sarath P., Wright, Amy E.
- Date Issued
- 2005
- PURL
- http://purl.flvc.org/FCLA/DT/3352941
- Subject Headings
- Polyketides, Sponges, Immunosuppressive agents, Antimitotic agents, Chemistry, Biology
- Format
- Document (PDF)
- Title
- On the presence of anatriaenes in Pachastrellidae (Porifera; Demospongiae): evidence for a new phylogenetic family concept.
- Creator
- Maldonado, Manuel, Harbor Branch Oceanographic Institute
- Date Issued
- 1996
- PURL
- http://purl.flvc.org/FCLA/DT/3352931
- Subject Headings
- Sponges, Demospongiae, Porifera, Phylogeny, Spicule (Anatomy)
- Format
- Document (PDF)
- Title
- Population dynamics of Vibrio spp. associated with marine sponge microcosms.
- Creator
- Hoffmann, M., Fischer, M., Ottesen, A., McCarthy, Peter J., Lopez, Jose V., Brown, E. W., Monday, S. R.
- Date Issued
- 2010
- PURL
- http://purl.flvc.org/FCLA/DT/3351984
- Subject Headings
- Sponges, Vibrio, Microbial ecology, RNA, Ribosomal, 16S
- Format
- Document (PDF)
- Title
- Comparison of the anaerobic microbiota of deep-water Geodia spp. and sandy sediments in the Straits of Florida.
- Creator
- Bruck, W. M., Bruck, T. B., Self, W. T., Reed, John K., Nitecki, S. S., McCarthy, Peter J.
- Date Issued
- 2010
- PURL
- http://purl.flvc.org/FCLA/DT/3351982
- Subject Headings
- Microbial ecology, Marine sediments, Florida, Straits of, Fluorescence in situ hybridization, Sponges
- Format
- Document (PDF)
- Title
- A new sesterterpene, Sch 599473, from a marine sponge, Ircinia sp.
- Creator
- Yang, Shu-Wei, Chan, Tze-Ming, Pomponi, Shirley A., Gonsiorek, W., Chen, Guodong, Wright, Amy E., Hipkin, W., Patel, Mahesh, Gullo, Vincent, Pramanik, B., Zavodny, P., Chu, Min
- Date Issued
- 2003
- PURL
- http://purl.flvc.org/FCLA/DT/3351980
- Subject Headings
- Sponges, Sesterterpenes, Marine natural products
- Format
- Document (PDF)
- Title
- Diversity and bioactivity of marine sponges of the Caribbean: the Turks and Caicos Islands.
- Creator
- Pomponi, Shirley A., Reed, John K., Wright, Amy E., McCarthy, Peter J.
- Date Issued
- 1996
- PURL
- http://purl.flvc.org/FCLA/DT/3351966
- Subject Headings
- Sponges--Turks and Caicos Islands, Bioactive compounds, Marine natural products, Sponges, Demospongiae
- Format
- Document (PDF)
- Title
- Associated bacterial communities of two deep-water sponges.
- Creator
- Olson, Julie B., McCarthy, Peter J.
- Date Issued
- 2005
- PURL
- http://purl.flvc.org/FCLA/DT/3350900
- Subject Headings
- Sponges, Gel electrophoresis, Heterotrophic bacteria, Host-bacteria relationships
- Format
- Document (PDF)