Current Search: Honors Student Theses (x) » Molecular biology (x) » Butyn, Amber. (x)
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Title
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Investigation of talin head-tail interactions.
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Creator
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Butyn, Amber., Harriet L. Wilkes Honors College
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Abstract/Description
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Talin is a ubiquitous, high-molecular-weight, flexible protein that plays a critical role in focal adhesions by activating, as well as connecting, integrins to the actin cytoskeleton. Talin's inactive auto-inhibitory state is speculated to be one of its modes of regulation inside the cell and is achieved through its head-tail interactions. In order to decipher the stability of this interaction, the head domain (residues 206-405) was cloned into a modified pET28m vector while the tail domains ...
Show moreTalin is a ubiquitous, high-molecular-weight, flexible protein that plays a critical role in focal adhesions by activating, as well as connecting, integrins to the actin cytoskeleton. Talin's inactive auto-inhibitory state is speculated to be one of its modes of regulation inside the cell and is achieved through its head-tail interactions. In order to decipher the stability of this interaction, the head domain (residues 206-405) was cloned into a modified pET28m vector while the tail domains (residues 1654-2344 and 2225-2344) were cloned into the pET32a vector to obtain octa-histidine tagged and un-tagged peptide, respectively. Neither co-expression nor pull-down using the His-tagged head domain was successful in purifying a stable head-tail complex. Our results indicate rather weak interactions between the talin head and rod domains and hence, under our experimental conditions, do not lead to a stable auto-inhibitory complex that can be purified for further studies.
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Date Issued
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2009
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PURL
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http://purl.flvc.org/FAU/209984
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Subject Headings
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Membrane proteins, Structure-activity relationships, Cell membranes, Physiology, Molecular biology, Cell interaction, Developmental cytology
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Format
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Document (PDF)