Current Search: FAU Graduate Student Research (x) » Esiobu, Nwadiuto (x)
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- Title
- Impact of Vitamin C on Genistein induced apoptosis in treatment of prostate cancer cells.
- Creator
- Famuyiwa, Toluleke, Boe, Andrew, Esiobu, Nwadiuto, Graduate College, Kumi-Diaka, James
- Abstract/Description
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Background: Prostate Cancer, in the absence of skin cancer, is the most prevalent type of cancer found in the male population. Reactive Oxygen Species (ROS) can promote cancer cell proliferation when they are at elevated levels. Vitamin C is a water-soluble antioxidant capable of inhibiting the formation of ROS. Genistein, an isoflavone found in plants, also possesses the ability to inhibit ROS formation. Objective To determine the potential therapeutic synergy between genistein and vitamin C...
Show moreBackground: Prostate Cancer, in the absence of skin cancer, is the most prevalent type of cancer found in the male population. Reactive Oxygen Species (ROS) can promote cancer cell proliferation when they are at elevated levels. Vitamin C is a water-soluble antioxidant capable of inhibiting the formation of ROS. Genistein, an isoflavone found in plants, also possesses the ability to inhibit ROS formation. Objective To determine the potential therapeutic synergy between genistein and vitamin C and investigate mechanism of action of genistein and/or vitamin C. Methods: Trypan blue assay was carried out to know the % of viable cells. Varying concentrations of genistein with a constant concentration of Vitamin C was used to treat LNCaP cells. After treatment of the cells with genistein and Vitamin C, MTT assay of the cancer cells was performed and absorbance read through an ELISA reader. This gives the values needed for interpreting cell viability after treatment. A statistical analysis performed to determine whether the obtained results are statistically significant. Results: The results obtained from our experiments are inconclusive with regards to the impact of Vitamin C on apoptotic cancer cell death following genistein treatment. However the combination of genistein and vitamin C was more efficient in tumor suppression than when the drugs were given separately. Conclusion: This study suggests that treatment of prostate cancer using genistein can be enhanced by adjuvant treatment with vitamin C. This study is of potential clinical success in reducing the cell death by necrosis.
Show less - Date Issued
- 2015
- PURL
- http://purl.flvc.org/fau/fd/FA00005876
- Format
- Document (PDF)
- Title
- Antibiotic resistance in the oral bacterial community.
- Creator
- Famuyiwa, Toluleke, Esiobu, Nwadiuto, Jia, Kailiang, Graduate College
- Abstract/Description
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Purpose: This study was designed to define the antibiotic resistance index of the cultivable oral microbiome to Amoxiacilin Clavulanic acid, Vancomycin, Ciprofloxacin, Clarithomycin, Chlorotetracyclin, Bacitracin, Kanamycin and Tobramycin using a new method adapted from the Kirby Bauer assay. Method: Oral wash samples were collected from 2 current smokers and 2 nonsmokers. Bacterial community were pelleted by centrifugation and used to create a lawn for the assay employing standard disk...
Show morePurpose: This study was designed to define the antibiotic resistance index of the cultivable oral microbiome to Amoxiacilin Clavulanic acid, Vancomycin, Ciprofloxacin, Clarithomycin, Chlorotetracyclin, Bacitracin, Kanamycin and Tobramycin using a new method adapted from the Kirby Bauer assay. Method: Oral wash samples were collected from 2 current smokers and 2 nonsmokers. Bacterial community were pelleted by centrifugation and used to create a lawn for the assay employing standard disk diffusion assay. Zones of inhibition and number of colonies in the zone were recorded. Mean values of inhibition zones were compared to established databases to draw conclusions. Result: The zones of inhibition of Bacitracin antibiotics shows that several bacteria from one of the non smokers were resistant to Bacitracin, while the smokers showed marked susceptibility. Conclusion: The new method developed in our lab yielded consistent set of data which serve as criteria for determining resistance of the oral microbiome to antibiotics. Quite remarkably, it is known that pathogenic beta Streptococci are susceptible to Bacitracin while non-pathogens are not; confirming that healthy persons harbor the healthy strains of streptococci. However the unanswered question is …. Could these normal biota pick up genes and become resistant too? Only time and human habits will decide but we have developed a baseline and an easy method for testing.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00005814
- Format
- Document (PDF)
- Title
- DNA Fingerprints and ribotypes of the oral microbiome can distinguish smokers and oral disease patients from healthy non smokers.
- Creator
- Chakraborty, Shreyasee, Persaud, Verneshia, Vergas, Sonya, Graduate College, Gautier, Gloryanne, Esiobu, Nwadiuto
- Abstract/Description
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Defining the bacterial communities associated with healthy status will permit rapid detection of shifts associated with disease and foster effective probiotic intervention. This study compares the PCR-RFLP of oral metagenomes and cultured bacterial community, as well as the bacterial diversity profile of smokers, non-smokers and oral disease patients. The goal was to evaluate the stability of bacteria associated with health and capture possible shifts potentially diagnostic of smokers and...
Show moreDefining the bacterial communities associated with healthy status will permit rapid detection of shifts associated with disease and foster effective probiotic intervention. This study compares the PCR-RFLP of oral metagenomes and cultured bacterial community, as well as the bacterial diversity profile of smokers, non-smokers and oral disease patients. The goal was to evaluate the stability of bacteria associated with health and capture possible shifts potentially diagnostic of smokers and oral disease. Oral wash samples were obtained from 5 healthy and 5 smokers twice, 3 months apart. Samples from 5 oral disease patients were also collected. Metagenomic and the genomic DNA of a cultured subset were amplified using primer 1492R and 27F.The generated 16SrRNA gene amplicons were used for arestriction digestion assay and bcloning with the Gene Hunter PCRTrap vector and pCR 4- TOPO cloning kits. The restriction fingerprints were statistically tested using Gel Compare II. The cloned 16S genes were sequenced using the ABI GeneAmp 9700 Thermal cycler. Sequences were analyzed by BLAST on the RDB II database and the HOMD. Sau 3AI and Alu I produced the best distinctive markers of 300 bp in healthy nonsmokers and 500bp in smokers in plate wash communities. The 16S sequence data suggest the presence of a core microbiota in all subjects mostly Streptococcus, Gamella, Candidatuse spp and confirmed that the smokers harbored highly diverse and distinct community Neisseria pharynges, Rothia mucilaginosa. Remarkably, there was a high stability of the fingerprints and diversity profile for smokers and nonsmokers after 3 months.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00005808
- Format
- Document (PDF)
- Title
- Environmental hotspots for antibiotic resistant bacteria arb.
- Creator
- Dawkins, Karim, Esiobu, Nwadiuto, Graduate College
- Abstract/Description
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The overuse and misuse of antibiotics in environmental and clinical settings have been a driving force for the prevalence of bacterial resistance. In constant interaction with these chemicals which can harm them, adaptively and inherently, bacteria have devised resistance mechanisms to combat the deleterious effects posed. In the presence of a particular antibiotic, it is expected there will be selection of resistant micro-organisms and their associated resistance genes if present. In this...
Show moreThe overuse and misuse of antibiotics in environmental and clinical settings have been a driving force for the prevalence of bacterial resistance. In constant interaction with these chemicals which can harm them, adaptively and inherently, bacteria have devised resistance mechanisms to combat the deleterious effects posed. In the presence of a particular antibiotic, it is expected there will be selection of resistant micro-organisms and their associated resistance genes if present. In this study, a set of 10 samples were taken from recreational beaches in Ft. Lauderdale, Miami and Hollywood and four different agricultural soils. These soils were enriched after being collected aseptically with three commonly used antibiotics; Ciprofloxacin, Tetracycline and Vancomycin to select for resistant organisms, which produced 29 total samples. A metagenomic analysis was done with 16S rDNA amplification with primers 27F and 1492R which produced 14 out of 29 amplicons producing the expected ~1400 bp fragment from the conserved SSU 16S rDNA region using Agarose gel electrophoresis. From these 14 samples amplified, a second PCR would be run from each enriched antibiotic sample with their respective antibiotic resistance primers eg. vanA-D, tetO and qnrA to identify the resistance genes present expecting that the antibiotic used for enrichment would select for the resistant organisms. Future work includes the sequencing of the amplified resistance genes to help identify novel genetic alterations indicative of new adaptive mechanisms.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00005810
- Format
- Document (PDF)