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- Title
- Effects of hippocampal impairment on rodent spatial and non-spatial memory.
- Creator
- Rios, Lisa, Lora, Joan C., Zhang, Gongliang, Stackman, Robert W., Graduate College
- Date Issued
- 2011-04-08
- PURL
- http://purl.flvc.org/fcla/dt/3164769
- Subject Headings
- Nerve Degeneration, Rodents, Space perception
- Format
- Document (PDF)
- Title
- Evidence for the emergence of relative navigational responding in male C57BL/6J mice in a land-based task.
- Creator
- Lora, Joan C., Stackman, Robert W., Graduate College
- Abstract/Description
-
We recently reported that male C57BL/6J mice navigate in spatial tasks, such as the Morris water maze MWM, by swimming in a particular direction to a location relative to poolbased cues, rather than to an absolute location defined by room-based cues. Neural mechanisms supporting this bias in rodents for relative responding rather than absolute responding in spatial tasks are not yet understood. Anterior thalamic neurons discharge according to the current directional heading of the animal. The...
Show moreWe recently reported that male C57BL/6J mice navigate in spatial tasks, such as the Morris water maze MWM, by swimming in a particular direction to a location relative to poolbased cues, rather than to an absolute location defined by room-based cues. Neural mechanisms supporting this bias in rodents for relative responding rather than absolute responding in spatial tasks are not yet understood. Anterior thalamic neurons discharge according to the current directional heading of the animal. The contribution of head direction HD cell activity to navigation has been difficult to elucidate. Selective inactivation of anterior thalamic nuclei ATN by microinfusion of muscimol or fluorophore-conjugated muscimol caused a near complete shift in preference from relative to absolute responding. Interestingly, inactivation of the dorsal CA1 region of the hippocampus did not affect relative responding. A land based version of the MWM has been developed to permit the recording of anterior thalamic HD cells during spatial search behavior. These experiments have been conducted to further examine the contribution of the HD cell activity to relative responding during spatial navigation.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00005834
- Format
- Document (PDF)
- Title
- Hippocampal place cell activity influenced by variations of the novel object recognition task in C57BL/6J mice.
- Creator
- Asgeirsdottir, Herborg Nanna, Cohen, Sarah J., Zhang, Gongliang, Munchow, Alcira H., Stackman, Robert W., Graduate College
- Date Issued
- 2013-04-12
- PURL
- http://purl.flvc.org/fcla/dt/3361265
- Subject Headings
- Hippocampus (Brain), Neurons, Mice
- Format
- Document (PDF)
- Title
- In vivo administration of a subtype selective activator of small conductance Ca2+ - activated K+ channels influences hippocampal-dependent spatial memory.
- Creator
- Beck, Robert, Kuchera, Claire Rice, Munchow, Alcira H., Stackman, Robert W., Graduate College
- Date Issued
- 2013-04-12
- PURL
- http://purl.flvc.org/fcla/dt/3361271
- Subject Headings
- Calcium-dependent potassium channels, Hippocampus (Brain), Mice, Memory
- Format
- Document (PDF)
- Title
- Investigating the contribution of small conductance Ca2+ activated K+ channels to the enhancement of Pavlovian fear learning and memory through administration of apamin.
- Creator
- Sanguinetti, Shannon, Stackman, Robert W., Graduate College
- Abstract/Description
-
Chronic activation of the amygdala through repetitive stressful events can lead to permanent hyper-excitability of its circuitry, which is known to be the root of a number of mood and anxiety disorders. Small conductance Ca2+-activated K+ (SK) channels expressed on lateral amygdala (LA) pyramidal neurons shape glutamatergic postsynaptic potentials and module NMDA receptor-dependent synaptic plasticity. When activated, SK channels reduce neuronal excitability and LTP. Induction of synaptic...
Show moreChronic activation of the amygdala through repetitive stressful events can lead to permanent hyper-excitability of its circuitry, which is known to be the root of a number of mood and anxiety disorders. Small conductance Ca2+-activated K+ (SK) channels expressed on lateral amygdala (LA) pyramidal neurons shape glutamatergic postsynaptic potentials and module NMDA receptor-dependent synaptic plasticity. When activated, SK channels reduce neuronal excitability and LTP. Induction of synaptic plasticity in LA pyramidal neurons causes PKAmediated internalization of SK channels from the postsynaptic density. The current study examined whether fear conditioning would affect the subsequent sensitivity of mice to novel fear memory encoding through SK channel blockade by the bee venom peptide, apamin. Naïve male C57BL/6J mice received a systemic injection of apamin or saline prior to exposure to a 1 tone (CS) - foot shock (US) conditioning protocol. Tone fear memory strength was examined 24 hours later. The next day, mice received the same or reversed treatments of saline or apamin and were conditioned to a novel CS and context. The influence of apamin on anxiety was also examined in the elevated plus maze to determine whether the drug was able to alter anxiety independent of conditioning. The fear conditioning results suggest that prior fear conditioning altered the sensitivity of mice to apamin-induced fear memory encoding during the second conditioning session. The plus maze results indicate that solely apamin does not alter anxiety, thus fear conditioning impairment in apamin-treated mice is not a reflection of drug effects alone.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00005852
- Format
- Document (PDF)
- Title
- Object-specific activity recorded from C57BL/6J mouse hippocampal CA1 neurons.
- Creator
- Asgeirsdottir, Herborg Nanna, Stackman, Robert W., Graduate College
- Abstract/Description
-
The rodent hippocampus is an essential neural substrate for spatial memory. This functional capacity is considered to rely upon a cognitive map that represents the location where relevant non-spatial items or objects are encountered and where specific events occur within a contextual or spatial reference frame. Place cell activity recorded from CA1 pyramidal neurons of the dorsal hippocampus of freely moving rodents is influenced by distal and proximal cues or items within an environment, and...
Show moreThe rodent hippocampus is an essential neural substrate for spatial memory. This functional capacity is considered to rely upon a cognitive map that represents the location where relevant non-spatial items or objects are encountered and where specific events occur within a contextual or spatial reference frame. Place cell activity recorded from CA1 pyramidal neurons of the dorsal hippocampus of freely moving rodents is influenced by distal and proximal cues or items within an environment, and increases when objects are placed into a familiar arena. Recently, the CA1 region of the rodent dorsal hippocampus was shown to play a vital role in object-in-context memory, and object memory independent of context; findings consistent with the cognitive map view. Here, we tested the influence of 3D objects on the spatial firing properties of CA1 neurons, since object-specific neuronal activity has not yet been fully established in mouse hippocampus. In vivo extracellular recordings from intermediate dorsal CA1 yielded simultaneous recordings of place cells and a pyramidal neuron demonstrating object-specific activity over two consecutive sessions with objects present. Higher frequency object-specific activity was recorded from the same mouse again 3 weeks later during a comparable task. Object-specific activity was observed only when the mouse explored objects in the arena, and was independent of spatial location or object identity. Recordings from more distal region of dorsal CA1, which receives input from proximal CA3, yielded two additional neurons that demonstrated comparable object-related activity. These results further support the involvement of the rodent hippocampus in non-spatial object memory.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00005801
- Format
- Document (PDF)
- Title
- Selective Activation of the SK1 Subtype of Small Conductance Ca2+-Activated K+Channels by 4-(2-Methoxyphenylcarbamoyloxymethyl)-piperidine-l-carboxylic Acid tert-Butyl Ester(GW542573X) in C57BL/6J Mice lmpairs Hippocampal-Dependent Memory.
- Creator
- Kuchera, Claire Rice, Stackman, Robert W., Graduate College
- Abstract/Description
-
Small conductance Ca2+ -activated K+ (SK) channels have physiological roles in learning and memory, intrinsic excitability, synaptic transmission and plasticity, and addiction. While SK2 and SK3 channels have been studied, the role of SK1 has not yet been determined due to the prior lack of gene-specific antibodies and agonists. SK1 are robustly expressed in the CA1 pyramidal neurons of the hippocampus and modulate their excitability by affecting afterhyperpolarization. SK1 subunits are only...
Show moreSmall conductance Ca2+ -activated K+ (SK) channels have physiological roles in learning and memory, intrinsic excitability, synaptic transmission and plasticity, and addiction. While SK2 and SK3 channels have been studied, the role of SK1 has not yet been determined due to the prior lack of gene-specific antibodies and agonists. SK1 are robustly expressed in the CA1 pyramidal neurons of the hippocampus and modulate their excitability by affecting afterhyperpolarization. SK1 subunits are only sited in the plasma membrane when co-expressed with SK2 or SK3. Co-expressed and co-assembled SK1, SK2, and SK3 subunits form functional apamin-sensitive channels. SK1 are not apamin selective, suggesting the overriding hypothesis that SK1 is a subunit of heteromeric SK channels that bind specific interacting proteins. We examined the effect of a new SK1 selective activator, GW542573X, on hippocampal dependent object memory in male C57BL/6J mice. The results showed that activating SK1 channels by systemic injection of the SK1 agonist GW542573X before the sample session, led to impaired object memory in mice 24 h later. Mice treated with GW542573X acquired the sample object exploration criterion in a similar latency as the vehicle-treated mice.GW542573X treated mice exhibited significantly less preference for exploring the novel object during the test session compared to the vehicle-treated mice. These results suggest that the SK1 activator disrupted the encoding of object memory without affecting the motivation to explore objects. This supports a role for SK1 in the modulation of hippocampal synaptic plasticity and hippocampal-dependent memory.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00005830
- Format
- Document (PDF)
- Title
- Uncovering the role of the rodent dorsal hippocampus in object recognition memory.
- Creator
- Cohen, Sarah J., Munchow, Alcira H., Stackman, Robert W., Graduate College
- Date Issued
- 2012-03-30
- PURL
- http://purl.flvc.org/fcla/dt/3342364
- Format
- Document (PDF)
- Title
- Unveiling the involvement of the rodent dorsal hippocampus in object recognition memory & investigating the role of context.
- Creator
- Cohen, Sarah J., Munchow, Alcira H., Asgeirsdottir, Herborg Nanna, Stackman, Robert W., Graduate College
- Date Issued
- 2013-04-12
- PURL
- http://purl.flvc.org/fcla/dt/3361918
- Subject Headings
- Hippocampus (Brain), Rodents, Memory
- Format
- Document (PDF)