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- Title
- Design considerations in high-throughput automation for biotechnology protocols.
- Creator
- Cardona, Aura, Roth, Zvi S., Florida Atlantic University, College of Engineering and Computer Science, Department of Computer and Electrical Engineering and Computer Science
- Abstract/Description
-
In this dissertation a computer-aided automation design methodology for biotechnology applications is proposed that leads to several design guidelines. Because of the biological nature of the samples that propagate in the automation line, a very specific set of environmental and maximum allowed shelf time conditions have to be followed to obtain good yield. In addition all biotechnology protocols require precise sequence of steps, the samples are scarce and the reagents are costly, so no...
Show moreIn this dissertation a computer-aided automation design methodology for biotechnology applications is proposed that leads to several design guidelines. Because of the biological nature of the samples that propagate in the automation line, a very specific set of environmental and maximum allowed shelf time conditions have to be followed to obtain good yield. In addition all biotechnology protocols require precise sequence of steps, the samples are scarce and the reagents are costly, so no waste can be afforded.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00004272, http://purl.flvc.org/fau/fd/FA00004272
- Subject Headings
- Biotechnological process control, Biotechnological process monitoring, Molecular biology -- Automation, Molecular biology -- Technique, Molecular cloning -- Technique, Pharmacognosy
- Format
- Document (PDF)
- Title
- Development and experimental testing of an amphibious vehicle.
- Creator
- Marquardt, Joseph G., College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
The development and experimental testing of the DUKW-Ling amphibious vehicle was performed during the first phase of an autonomous amphibious vehicle system development project. The DUKW-Ling is a 1/7th scale model of a cargo transport concept vehicle. The vehicle was tested in the three regions it is required to operate: land, sea and the surf zone region. Vehicle characteristics such as turning radii, yaw rate and velocities were found for different motor inputs on land and water. Also,...
Show moreThe development and experimental testing of the DUKW-Ling amphibious vehicle was performed during the first phase of an autonomous amphibious vehicle system development project. The DUKW-Ling is a 1/7th scale model of a cargo transport concept vehicle. The vehicle was tested in the three regions it is required to operate: land, sea and the surf zone region. Vehicle characteristics such as turning radii, yaw rate and velocities were found for different motor inputs on land and water. Also, because a vehicle navigating the surf zone is a new area of research that lacks experimental data the vehicle was tested in the breaking waves of the surf zone and its motion characteristics were found, as well as the drivetrain forces required to perform this transition. Maneuvering tests provided data that was used to estimate a model for future autonomous control efforts for both land and water navigation.
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3342208
- Subject Headings
- Motor vehicles, Amphibious, Design and construction, Naval architecture
- Format
- Document (PDF)
- Title
- Development of Crown Ether Nucleophilic Catalysts (CENCs) and their Application in Rapid Fluorination of Silicon for PET Imaging & Diversification Reactions of γ-Silyl Allenyl Esters to All-carbon Quaternary Stereogenic Centers.
- Creator
- Jana, Susovan, Lepore, Salvatore D., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
In this dissertation, we discuss the development of new phase transfer agents, which are capable of rapid fluorination of silicon. These are 18-C-6 derivatives containing a hydroxyl group in the side arm (podand), also known as C-pivot lariats. The syntheses of these lariats including several that have not been previously reported and their efficient purification are described. The synthesis route leads to a robust and generalized approach to obtain these lariats on the gram scale. These...
Show moreIn this dissertation, we discuss the development of new phase transfer agents, which are capable of rapid fluorination of silicon. These are 18-C-6 derivatives containing a hydroxyl group in the side arm (podand), also known as C-pivot lariats. The syntheses of these lariats including several that have not been previously reported and their efficient purification are described. The synthesis route leads to a robust and generalized approach to obtain these lariats on the gram scale. These agents were initially designed for applications in positron emission tomography (PET). In this medical imaging modality, tracer agents containing silicon have found promising utility as fluoride receptors for more rapid radiolabeling. Phase transfer agents are generally required for 18F-labeling due to the low solubility in organic reaction media and reactivity of cyclotron-generated [18F]potassium fluoride. We envisioned that 18-C-6 derivatives may serve as both phase transfer agents as well as nucleophilic catalysts (CENCs). In this conception, CENCs were rapidly pre-complexed with KF followed by silicon fluorination, which takes advantage of a previously established silicon dianion mechanism. In collaboration with researchers at the NIH, we studied the effect of various linkers connecting the metal chelating unit to the nucleophilic hydroxyl group on the radiofluorination of silicon under mild condition. A hydrolysis resistant aryl silicon fragment has also been developed that contains various functional groups for convenient attachment to the potential PET radiotracer agents. In a second project, we demonstrate the unique reactivity of γ-silyl allenyl esters. Taking advantage of the silyl group as a fluoride acceptor, these allenoates readily underwent addition to a variety of carbon electrophiles, including aryl fluorides, to afford all-carbon quaternary centers bearing an ethynyl group. Surprisingly, in the presence of aldehydes, exclusive bis-substitution occurs at the γ-position to afford the dicarbinol. Details relating to reaction optimization and substrate scope for both the reactions are presented. Dicarbinol allenes were subsequently converted to highly substituted δ-lactones, a novel 6-hydro-2-pyrone as single diastereomers.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FA00013073
- Subject Headings
- Phase-transfer catalysis., Silicon., Positron-Emission Tomography., Crown ethers., Radioactive tracers., Fluorination.
- Format
- Document (PDF)
- Title
- Development of Nucleophile Assisting Leaving Groups (NALGs) and new stereoselective reactions using titanium(IV) reagents.
- Creator
- Mondal, Deboprosad., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
We report here the development of very efficient sulfonate based leaving groups, termed Nucleophile Assisting Leaving Groups (NALGs), to accelerate the rate of nucleophilic substitution reactions involving poor nucleophiles and/or substrates traditionally considered too hindered to undergo nucleophilic attack. Indeed NALGs have shown exceptional ability in improving rate of nucleophilic substitution reactions. New very mild stereoretentive halogenations and azidation reactions have also been...
Show moreWe report here the development of very efficient sulfonate based leaving groups, termed Nucleophile Assisting Leaving Groups (NALGs), to accelerate the rate of nucleophilic substitution reactions involving poor nucleophiles and/or substrates traditionally considered too hindered to undergo nucleophilic attack. Indeed NALGs have shown exceptional ability in improving rate of nucleophilic substitution reactions. New very mild stereoretentive halogenations and azidation reactions have also been developed for secondary cyclic alcohols using NALGs involving titanium(IV) reagents. This reaction is particularly significant since the carbon-halogen bond is found widely in natural products and is used extensively as a synthesis intermediate. Azide is also a synthetically important functional group from which a variety of biologically important functional groups are conveniently obtained. Though stereoretentive chlorination and bromination reactions are known, we have developed, for the first time, a stereoretentive azidation reaction using titanium(IV) azide, a reagent not previously used in organic synthesis. During our development of stereoretentive reactions, we eventually developed very efficient, mild, two-step one-pot stereoretentive halogenations (chlorination and bromination) using titanium(IV) halides as catalysts or stoichiometric reagents. These reactions were found to be particularly efficient for cyclic alcohols. An efficient one pot stereoretentive amidation reaction for secondary cyclic alcohols is also reported. The important features of this reaction are that, for the first time, chlorosulfite (prepared in situ from alcohol using thionylchloride) has been used as a leaving group and titanium(IV) fluoride as an activator., Utilization of those two reagents is unique as thionylchloride has never been used for nucleophilic substitution reactions except in chlorination procedures. In addition, this work has found new and creative applications for titanium (IV) fluoride, a reactant rarely used in organic synthesis. Further exploiting the unique reactivity of titanium(IV), reactions of alkenes with various nucleophiles have been developed with this reagent in both catalytic and stoichiometric quantities. It was observed that a-substituted aromatic conjugated alkenes dimerize to generate important indan class of compounds which are very important in the polymer industry. In addition, non conjugated unactivated alkenes react with various nucleophiles to yield the adduct.
Show less - Date Issued
- 2010
- PURL
- http://purl.flvc.org/FAU/3159264
- Subject Headings
- Chemical reaction, Condition and laws of, Organic compounds, Synthesis, Chemical templates, Intermediates (Chemistry), Organotransition metal compounds, Organic reaction mechanisms
- Format
- Document (PDF)
- Title
- Development and implementation of an adaptive controller for station keeping of small outboard-powered vessels.
- Creator
- Fisher, Aaron D., College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
In this thesis multiple controllers are developed which command a small boat with twin tied outboard motors to hold a desired position. In the process of developing a controller to hold a position, controllers were first developed which follow a desired heading or path over ground with the motors outputting constant thrust. These heading and path following controllers were tuned and tested in a numerical simulation, then validated on the R/V Lee and Ocean Power vessels through sea trials in...
Show moreIn this thesis multiple controllers are developed which command a small boat with twin tied outboard motors to hold a desired position. In the process of developing a controller to hold a position, controllers were first developed which follow a desired heading or path over ground with the motors outputting constant thrust. These heading and path following controllers were tuned and tested in a numerical simulation, then validated on the R/V Lee and Ocean Power vessels through sea trials in the Atlantic Ocean. After successful path following trials were performed, station keeping algorithms were developed and tuned in the numerical simulation, now with heading and thrust of the vessel both being variables to be controlled. After tuning in the numerical simulation, the Ocean power vessel was outfitted with systems for controlling throttle and steering with sea trials conducted in the Atlantic Ocean for station keeping.
Show less - Date Issued
- 2010
- PURL
- http://purl.flvc.org/FAU/2975247
- Subject Headings
- Ship handling, Stability of ships, Fracture mechanics, Boats and boating, Design, PID controllers, Computer simulation
- Format
- Document (PDF)
- Title
- Development of Linear Feature Based Non-Contact Bridge Deflection Monitoring System.
- Creator
- Khamaru, Satarupa, Nagarajan, Sudhagar, Florida Atlantic University, College of Engineering and Computer Science, Department of Civil, Environmental and Geomatics Engineering
- Abstract/Description
-
In any infrastructure project, monitoring and managing the built assets is an important task. Structural Health Monitoring (SHM) is meant for continuous assessment of safety and serviceability of a structure and its elements. SHM has taken a leading role in the field of structural engineering and has become very popular in recent age. Bridge deflection is the basic evaluation index to examine the health status of a bridge structure. The existing bridge monitoring systems have several...
Show moreIn any infrastructure project, monitoring and managing the built assets is an important task. Structural Health Monitoring (SHM) is meant for continuous assessment of safety and serviceability of a structure and its elements. SHM has taken a leading role in the field of structural engineering and has become very popular in recent age. Bridge deflection is the basic evaluation index to examine the health status of a bridge structure. The existing bridge monitoring systems have several drawbacks. Hence, a new methodological approach has been proposed to overcome the limitations of traditional contact-based bridge deflection monitoring system and other non-contact based system. This study developed a non-contact linear feature based Deflection Monitoring System (DMS) using Terrestrial Laser Scanning (TLS) and cameras for timber railroad bridges. The process and detailed workflow of building the DMS, its components and sensors involved are discussed here. The efficiency of this DMS is validated against a deflectometer.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FA00013081
- Subject Headings
- Structural health monitoring., Bridges--Evaluation., Deflection.
- Format
- Document (PDF)
- Title
- Development of Smart Phone-based Automated Microfluidic-ELISA For Human Immunodefciency Virus 1.
- Creator
- Coarsey, Chad Thomas, Asghar, Waseem, Florida Atlantic University, College of Engineering and Computer Science, Department of Computer and Electrical Engineering and Computer Science
- Abstract/Description
-
The majority of HIV prevalence is found in Sub-Saharan Africa with 36.9 mil- lion living with HIV/AIDS. The cultural implications such as patient non-compliance or denial of available routine medical care can potentially cause limitations on the ef- fectiveness of detecting such virulent pathogens and manage chronic disease. The lack of access to healthcare and further socioeconomic impacts hinder the ability to ade- quately diagnose and treat infection in resource-limited settings....
Show moreThe majority of HIV prevalence is found in Sub-Saharan Africa with 36.9 mil- lion living with HIV/AIDS. The cultural implications such as patient non-compliance or denial of available routine medical care can potentially cause limitations on the ef- fectiveness of detecting such virulent pathogens and manage chronic disease. The lack of access to healthcare and further socioeconomic impacts hinder the ability to ade- quately diagnose and treat infection in resource-limited settings. Intervention through diagnosis and treatment helps prevent the spread of transmission, where pre-exposure prophylaxis or active disease prevention measures are not readily available. The cur- rent gold standard for HIV detection is by molecular detection; Reverse-Transcription Polymerase Chain Reaction is widely used that employs cycles of temperature condi- tions that require a thermal cycling platform and typically laboratory space for RNA extraction separate from RT-PCR space required. Serological detection can be ad- vantageous for surveillance and screening, Lateral Flow Assays and Enzyme-Linked Immunosorbent Assay (ELISA) can detect a viral protein (antigen) or antibodies. The ELISA can require at least 12 hours of assay preparation and takes a diagnostic laboratory many resources to run. There is need to develop Point-of-Care (POC) testing that can potentially be used for decentralized testing that can leverage ex- isting technologies such as smart phone capability and routine medical or diagnostic tests with cutting edge applications leveraging micro uidics, nanotechnology and in- tegrated circuit design. Such technologies allow for automated, rapid turnaround and cost-e ective diagnosis of HIV, where these assays could potentially be read- ily deployed. It is such technology that can potentially change the way diagnostics are performed, as POC technology can be rapidly disseminated, enable decentralized testing and, is user-friendly. A novel smart phone-enabled automated magnetic bead- based platform was developed for a micro uidic ELISA for HIV-1 detection at the POC to meet this demand.
Show less - Date Issued
- 2017
- PURL
- http://purl.flvc.org/fau/fd/FA00005945
- Subject Headings
- Dissertations, Academic -- Florida Atlantic University
- Format
- Document (PDF)
- Title
- Development of MnO2 Hollow Nanoparticles for Drug Delivery.
- Creator
- Greene, Allison, Kang, Yunqing, Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
This thesis reports the development of a novel drug delivery system consisting of hollow nanoparticles, formed from manganese dioxide (δ-MnO2) sheets, that are coated with polydopamine and folic acid to selectively target cancer cells. The biodegradability and colloidal stability of the uncoated hollow nanoparticles were investigated in comparison to solid MnO2 nanoparticles and graphene oxide sheets. The MnO2 hollow nanoparticles degraded at a faster rate and seem to have a higher surface...
Show moreThis thesis reports the development of a novel drug delivery system consisting of hollow nanoparticles, formed from manganese dioxide (δ-MnO2) sheets, that are coated with polydopamine and folic acid to selectively target cancer cells. The biodegradability and colloidal stability of the uncoated hollow nanoparticles were investigated in comparison to solid MnO2 nanoparticles and graphene oxide sheets. The MnO2 hollow nanoparticles degraded at a faster rate and seem to have a higher surface area and better colloidal dispersion than solid MnO2 nanoparticles. Xanthan gum was proven to improve colloidal dispersion of these hollow nanoparticles and were used for further cell studies. In this study, cancer and healthy cells were treated with coated hollow nanoparticles, and results indicate that this novel hollow nanoparticle may preferentially target and kill cancer cells. Particle aggregation has shown to be toxic to cells. Further studies with this novel drug delivery system may lead to a groundbreaking solution to targeted cancer therapy.
Show less - Date Issued
- 2020
- PURL
- http://purl.flvc.org/fau/fd/FA00013513
- Subject Headings
- Drug Delivery Systems, Nanoparticles, Manganese dioxide, Xanthan gum, Cancer cells
- Format
- Document (PDF)
- Title
- Development of A Portable Impedance Based Flow Cytometer for Diagnosis of Sickle Cell Disease.
- Creator
- Dieujuste, Darryl, Zhuang, Hanqi, Du, Sarah, Florida Atlantic University, College of Engineering and Computer Science, Department of Computer and Electrical Engineering and Computer Science
- Abstract/Description
-
Sickle cell disease is an inherited blood cell disorder that affects about 100,000 people in the US and results in high cost of medical care exceeding $1.1 billion annually. Sickle cell patients suffer from unpredictable, painful vaso-occlusive crises. Portable, costeffective approaches for diagnosis and monitoring sickle blood activities are important for a better management of the disease and reducing the medical cost. In this research, a mobile application controlled, impedance-based flow...
Show moreSickle cell disease is an inherited blood cell disorder that affects about 100,000 people in the US and results in high cost of medical care exceeding $1.1 billion annually. Sickle cell patients suffer from unpredictable, painful vaso-occlusive crises. Portable, costeffective approaches for diagnosis and monitoring sickle blood activities are important for a better management of the disease and reducing the medical cost. In this research, a mobile application controlled, impedance-based flow cytometer is developed for the diagnosis of sickle cell disease. Calibration of the portable device is performed using a component of known impedance value. The preliminary test results are then compared to those obtained by a commercial benchtop impedance analyzer for further validation. With the developed portable flow cytometer, experiments are performed on two sickle cell samples and a healthy cell sample. The acquired results are subsequently analyzed with MATLAB scripts to extract single-cell level impedance information as well as statistics of different cell conditions. Significant differences in cell impedance signals are observed between sickle cells and normal cells, as well as between sickle cells under hypoxia and normoxia conditions.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FA00013145
- Subject Headings
- Sickle cell disease, Sickle cell anemia--Diagnosis, Flow cytometry--Diagnostic use, Mobile Applications
- Format
- Document (PDF)
- Title
- Development and testing of a measure of Alzheimer’s disease knowledge in a rural Appalachian community.
- Creator
- Weise, Lisa K., Williams, Christine L., Christine E. Lynn College of Nursing
- Abstract/Description
-
Rural West Virginia has a very high percentage of older adults. The age-related disease of Alzheimer’s threatens the health of older Appalachians, yet research on Alzheimer’s disease (AD) in this population is scarce. In order to improve screening rates for cognitive impairment, Appalachians need to understand their vulnerability. The first step would be to assess their knowledge about AD but a suitable AD knowledge test has not been developed. The purpose of this study was to test the...
Show moreRural West Virginia has a very high percentage of older adults. The age-related disease of Alzheimer’s threatens the health of older Appalachians, yet research on Alzheimer’s disease (AD) in this population is scarce. In order to improve screening rates for cognitive impairment, Appalachians need to understand their vulnerability. The first step would be to assess their knowledge about AD but a suitable AD knowledge test has not been developed. The purpose of this study was to test the reliability and validity of a new measure of knowledge about AD that is culturally congruent, and to examine factors that may predict AD knowledge in this rural population. A correlational descriptive study was conducted with 240 participants from four samples of older adults in south central rural Appalachian West Virginia using surveys and face-to-face interviews. Results from tests for stability, reliability including Rasch modeling, discrimination and point biserial indices, and concurrent, divergent, and construct validity were favorable. Findings were that although more diversity in test item difficulty is needed, the test discriminated well between persons with higher and lower levels of education [F(2, 226) = 170.51, p = .001]. Using multiple regression, the predictors of AD knowledge included caregiver status, miles from a healthcare provider, gender, and education; (R2=.05, F(4,187) = 2.65, p =. 04). Only years of education accounted for a significant proportion of unique variance in predicting the total BKAD score (t = 2.14, p =. 03). Implications include the need for further tool refinement, testing for health literacy, coordination with recent statewide efforts to educate the public regarding AD, and community based participatory research in designing culturally effective education programs that will ultimately increase screening and detection of Alzheimer’s disease in rural populations.
Show less - Date Issued
- 2013
- PURL
- http://purl.flvc.org/fau/fd/FA0004074
- Subject Headings
- Alzheimer's disease -- Diagnosis -- Social aspects, Alzheimer's disease -- Research -- Methodology, Health literacy -- West Virginia -- Appalachian Region, Southern, West Virginia -- Appalachian Region, Southern -- Social aspects
- Format
- Document (PDF)
- Title
- Development and testing of an instrument to measure holistic attributes of nurse practitioner care.
- Creator
- Kinchen, Elizabeth V., Lange, Bernadette, Florida Atlantic University, Christine E. Lynn College of Nursing
- Abstract/Description
-
With passage of the Patient Protection and Affordable Care Act (PPACA) and the publication of the Institute of Medicine’s report on the future of nursing, nurses are slated to take on an expanded role in primary healthcare delivery in the near future. Nurse practitioners, in particular, will be instrumental in filling the gap in primary care availability engendered by the increasing specialization of physician practice and increased access to healthcare made possible by the provisions of the...
Show moreWith passage of the Patient Protection and Affordable Care Act (PPACA) and the publication of the Institute of Medicine’s report on the future of nursing, nurses are slated to take on an expanded role in primary healthcare delivery in the near future. Nurse practitioners, in particular, will be instrumental in filling the gap in primary care availability engendered by the increasing specialization of physician practice and increased access to healthcare made possible by the provisions of the PPACA. The need for this study was identified through gaps in the literature related to nurse practitioner practice; specifically, the paucity of quantitative research regarding patients’ perspectives of core holistic nursing values in nurse practitioner care, and, since nursing care is by definition and tradition holistic in nature, this inquiry attempted to quantify the degree to which nurse practitioner care upholds and preserves core holistic nursing values.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00004297, http://purl.flvc.org/fau/fd/FA00004297
- Subject Headings
- Holistic nursing, Nurse and patient, Nurse practitioners -- Training of, Nursing -- Philosophy, Nursing -- Practice, Primary care (Medicine) -- Practice
- Format
- Document (PDF)
- Title
- Development of an integrated computational tool for design and analysis of composite turbine blades under ocean current loading.
- Creator
- Zhou, Fang., College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
A computational tool has been developed by integrating National Renewable Energy Laboratory (NREL) codes, Sandia National Laboratories' NuMAD, and ANSYS to investigate a horizontal axis composite ocean current turbine. The study focused on the design, analysis, and life prediction of composite blade considering random ocean current, cyclic rotation, and hurricane-driven ocean current. A structural model for a horizontal axis FAU research OCT blade was developed. Following NREL codes were used...
Show moreA computational tool has been developed by integrating National Renewable Energy Laboratory (NREL) codes, Sandia National Laboratories' NuMAD, and ANSYS to investigate a horizontal axis composite ocean current turbine. The study focused on the design, analysis, and life prediction of composite blade considering random ocean current, cyclic rotation, and hurricane-driven ocean current. A structural model for a horizontal axis FAU research OCT blade was developed. Following NREL codes were used: PreCom, BModes, ModeShape, AeroDyn and FAST. PreComp was used to compute section properties of the OCT blade. BModes and ModeShape calculated the mode shapes of the blade. Hydrodynamic loading on the OCT blade was calculated by modifying the inputs to AeroDyn and FAST. These codes were then used to obtain the dynamic response of the blade, including blade tip displacement, normal force (FN) and tangential force (FT), flap and edge bending moment distribution with respect to blade rotation.
Show less - Date Issued
- 2013
- PURL
- http://purl.flvc.org/fcla/dt/3362582
- Subject Headings
- Structural dynamics, Fluid dynamics, Marine turbines, Mathematical models, Turbines, Blades, Design and construction
- Format
- Document (PDF)
- Title
- Development of functional relationships between radar and rain gage data using inductive modeling techniques.
- Creator
- Peters, Delroy., College of Engineering and Computer Science, Department of Civil, Environmental and Geomatics Engineering
- Abstract/Description
-
Traditional methods such as distance weighing, correlation and data driven methods have been used in the estimation of missing precipitation data. Also common is the use of radar (NEXRAD) data to provide better spatial distribution of precipitation as well as infilling missing rain gage data. Conventional regression models are often used to capture highly variant nonlinear spatial and temporal relationships between NEXRAD and rain gage data. This study aims to understand and model the...
Show moreTraditional methods such as distance weighing, correlation and data driven methods have been used in the estimation of missing precipitation data. Also common is the use of radar (NEXRAD) data to provide better spatial distribution of precipitation as well as infilling missing rain gage data. Conventional regression models are often used to capture highly variant nonlinear spatial and temporal relationships between NEXRAD and rain gage data. This study aims to understand and model the relationships between radar (NEXRAD) estimated rainfall data and the data measured by conventional rain gages. The study is also an investigation into the use of emerging computational data modeling (inductive) techniques and mathematical programming formulations to develop new optimal functional approximations. Radar based rainfall data and rain gage data are analyzed to understand the spatio-temporal associations, as well as the effect of changes in the length or availability of data on the models. The upper and lower Kissimmee basins of south Florida form the test-bed to evaluate the proposed and developed approaches and also to check the validity and operational applicability of these functional relationships among NEXRAD and rain gage data for infilling of missing data.
Show less - Date Issued
- 2008
- PURL
- http://purl.flvc.org/FAU/166454
- Subject Headings
- Weather control, Mathematical models, Radar meteorology, Technological innovations, Precipitation (Meteorology), Measurement, Weather forecasting, Technological innovations
- Format
- Document (PDF)
- Title
- Developmental and Protective Mechanisms of the Ocular Lens.
- Creator
- Chauss, Daniel C., Kantorow, Marc, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biomedical Science
- Abstract/Description
-
The vertebrate eye lens functions to focus light onto the retina to produce vision. The lens is composed of an anterior monolayer of cuboidal epithelial cells that overlie a core of organelle free fiber cells. The lens develops and grows throughout life by the successive layering of lens fiber cells via their differentiation from lens epithelial cells. Lens developmental defect and damage to the lens are associated with cataract formation, an opacity of the lens that is a leading cause of...
Show moreThe vertebrate eye lens functions to focus light onto the retina to produce vision. The lens is composed of an anterior monolayer of cuboidal epithelial cells that overlie a core of organelle free fiber cells. The lens develops and grows throughout life by the successive layering of lens fiber cells via their differentiation from lens epithelial cells. Lens developmental defect and damage to the lens are associated with cataract formation, an opacity of the lens that is a leading cause of visual impairment worldwide. The only treatment to date for cataract is by surgery. Elucidating those molecules and mechanisms that regulate the development and lifelong protection of the lens is critical toward the development of future therapies to prevent or treat cataract. To determine those molecules and mechanisms that may be important for these lens requirements we employed high-throughput RNA sequencing of microdissected differentiation statespecific lens cells to identify an extensive range of transcripts encoding proteins expressed by these functionally distinct cell types. Using this data, we identified differentiation state-specific molecules that regulate mitochondrial populations between lens epithelial cells that require the maintenance of a functional population of mitochondria and lens fiber cells that must eliminate their mitochondria for their maturation. In addition, we discovered a novel mechanism for how lens epithelial cells clear apoptotic cell debris that could arise from damage to the lens and found that UVlight likely compromises this system. Moreover, the data herein provide a framework to determine novel lens cell differentiation state-specific mechanisms. Future studies are required to determine the requirements of the identified molecules and mechanisms during lens development, lens defense against damage, and cataract formation.
Show less - Date Issued
- 2016
- PURL
- http://purl.flvc.org/fau/fd/FA00004577
- Subject Headings
- Eye--Diseases--Etiology., Cell differentiation., Cellular signal transduction., Protein folding., Mitochondrial pathology., Cellular control mechanisms., Apoptosis., Oxidative stress--Prevention.
- Format
- Document (PDF)
- Title
- Development, evaluation, and application of spatio-temporal wading bird foraging models to guide everglades restoration.
- Creator
- Beerens, James M., Noonburg, Erik G., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
In south Florida, the Greater Everglades ecosystem supports sixteen species of wading birds. Wading birds serve as important indicator species because they are highly mobile, demonstrate flexible habitat selection, and respond quickly to changes in habitat quality. Models that establish habitat relationships from distribution patterns of wading birds can be used to predict changes in habitat quality that may result from restoration and climate change. I developed spatio-temporal species...
Show moreIn south Florida, the Greater Everglades ecosystem supports sixteen species of wading birds. Wading birds serve as important indicator species because they are highly mobile, demonstrate flexible habitat selection, and respond quickly to changes in habitat quality. Models that establish habitat relationships from distribution patterns of wading birds can be used to predict changes in habitat quality that may result from restoration and climate change. I developed spatio-temporal species distribution models for the Great Egret, White Ibis, and Wood Stork over a decadal gradient of environmental conditions to identify factors that link habitat availability to habitat use (i.e., habitat selection), habitat use to species abundance, and species abundance (over multiple scales) to nesting effort and success. Hydrological variables (depth, recession rate, days since drydown, reversal, and hydroperiod) over multiple temporal scales and with existing links to wading bird responses were used as proxies for landscape processes that influence prey availability (i.e., resources). In temporal foraging conditions (TFC) models, species demonstrated conditional preferences for resources based on resource levels at differing temporal scales. Wading bird abundance was highest when prey production from optimal periods of wetland inundation was concentrated in shallow depths. Similar responses were observed in spatial foraging conditions (SFC) models predicting spatial occurrence over time, accounting for spatial autocorrelation. The TFC index represents conditions within suitable depths that change daily and reflects patch quality, whereas the SFC index spatially represents suitability of all cells and reflects daily landscape patch abundance. I linked these indices to responses at the nest initiation and nest provisioning breeding phases from 1993-2013. The timing of increases and overall magnitude of resource pulses predicted by the TFC in March and April were strongly linked to breeding responses by all species. Great Egret nesting effort and success were higher with increases in conspecific attraction (i.e., clustering). Wood Stork nesting effort was closely related to timing of concurrently high levels of patch quality (regional scale) and abundance (400-m scale), indicating the importance of a multi-scaled approach. The models helped identify positive and negative changes to multi-annual resource pulses from hydrological restoration and climate change scenarios, respectively.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00004078, http://purl.flvc.org/fau/fd/FA00004078
- Subject Headings
- Everglades National Park (Fla.) -- Environmental conditions, Restoration ecology -- Florida -- Everglades National Park, Water birds -- Florida -- Geographical distribution, Water birds -- Habitat -- Florida -- Everglades National Park, Wetland restoration -- Florida -- Everglades National Park
- Format
- Document (PDF)
- Title
- Development of inhibition as a function of the presence of an intentional agent.
- Creator
- King, Ashley., Charles E. Schmidt College of Science, Department of Psychology
- Abstract/Description
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This thesis examined the developmental differences in inhibition and theory-of-mind of 4-8 year olds as a function of the suggested presence of a supernatural agent. All children played four games designed to assess their current level of inhibition and theory-of-mind performance; Children in the experimental condition, only, were also introduced to an invisible Princess Alice and were told that she was watching during the games. Following these measures, all children engaged in a resistance...
Show moreThis thesis examined the developmental differences in inhibition and theory-of-mind of 4-8 year olds as a function of the suggested presence of a supernatural agent. All children played four games designed to assess their current level of inhibition and theory-of-mind performance; Children in the experimental condition, only, were also introduced to an invisible Princess Alice and were told that she was watching during the games. Following these measures, all children engaged in a resistance-to-temptation task to determine any differences in inhibition resulting from Princess Alice's suggested presence. I found that children exhibiting a well-developed theory-of-mind were more likely to express belief in Princess Alice than were children lacking this cognitive ability. This research provided support that cognitive maturity, rather than immaturity, may be necessary for children to express belief in novel supernatural agents, and highlighted the importance of context as a mediating factor in children's behavioral inhibition.
Show less - Date Issued
- 2009
- PURL
- http://purl.flvc.org/FAU/227977
- Subject Headings
- Child psychopathology, Physiological aspects, Cognition in children, Philosophy of mind in children, Inhibition
- Format
- Document (PDF)
- Title
- Development of an Arduino-based 3D printed 6DOF robotic phantom and a MATLAB-based software for Radiation Therapy Quality Assurance.
- Creator
- Rahman, Md Mushfiqur, Leventouri, Theodora, Shang, Charles, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Physics
- Abstract/Description
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Quality Assurance (QA) for medical linear accelerators (linac) is the primary concern in external beam radiation therapy. In this research, we have developed a MATLAB-based software named Quality Assurance for Linacs (QALMA), which is unique, due to cost-effectiveness, user friendly interface, and customizability. It includes five modules to perform different QA tests: Star Shot analysis, Picket Fence test, Winston-Lutz test, MLC log file analysis, and verification of light & radiation field...
Show moreQuality Assurance (QA) for medical linear accelerators (linac) is the primary concern in external beam radiation therapy. In this research, we have developed a MATLAB-based software named Quality Assurance for Linacs (QALMA), which is unique, due to cost-effectiveness, user friendly interface, and customizability. It includes five modules to perform different QA tests: Star Shot analysis, Picket Fence test, Winston-Lutz test, MLC log file analysis, and verification of light & radiation field coincidence. We also pay attention to quality assurance of 6DOF treatment couch that plays a very important role in radiation therapy. We developed an Arduino based 3D printed 6DOF robotic phantom to check the accuracy of the treatment couch. This robotic phantom was experimentally validated under clinical standards, and customizable upon requirements of the quality assurance Task. The current features of this robotic phantom open development opportunities beyond the specific couch application, such as organs motion simulation.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FA00013165
- Subject Headings
- Radiation therapy, Radiotherapy--Quality control, Arduino (Computer language), MATLAB
- Format
- Document (PDF)
- Title
- Development of a remote sensing technique for woody vegetation in Rotenberger Wildlife Management Area.
- Creator
- Franklin, Sarah., Charles E. Schmidt College of Science, Department of Geosciences
- Abstract/Description
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The Florida Fish and Wildlife Conservation Commission lacks a viable method for monitoring woody vegetation in expansive wetland communities, such as the Florida Everglades. This study used aerial photographs of Rotenberger Wildlife Management Area in southeastern Palm Beach County, Florida to develop techniques for remotely monitoring changes in woody vegetation. Imagery from 2006, 2008, and 2010 were classified into woody and non-woody categories using Adobe Photoshop's Magic Wand Tool....
Show moreThe Florida Fish and Wildlife Conservation Commission lacks a viable method for monitoring woody vegetation in expansive wetland communities, such as the Florida Everglades. This study used aerial photographs of Rotenberger Wildlife Management Area in southeastern Palm Beach County, Florida to develop techniques for remotely monitoring changes in woody vegetation. Imagery from 2006, 2008, and 2010 were classified into woody and non-woody categories using Adobe Photoshop's Magic Wand Tool. Selection was performed with a bias toward over classification, as project objectives required identifying as many trees as possible. Classified pixels in Time 1 within 4 feet (2 pixels) of classified pixels from Time 2 were considered the same canopy. Overall accuracy for the study was 98%.
Show less - Date Issued
- 2011
- PURL
- http://purl.flvc.org/FAU/3175018
- Subject Headings
- Environmental monitoring, Remote sensing, Vegetation dynamics, Ecosystem management
- Format
- Document (PDF)
- Title
- Developmental delays in methionine sulfoxide reductase mutants in Drosophila Melanogaster.
- Creator
- Hausman, William, Binninger, David, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
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Aging is a biological process that has many detrimental effects due to the accumulation of oxidative damage to key biomolecules due to the action of free radicals. Methionine sulfoxide reductase (Msr) functions to repair oxidative damage to methionine residues. Msr comes in two forms, MsrA and MsrB, each form has been shown to reduce a specific enantiomer of bound and free oxidized methionine. Effects of Msr have yet to be studied in the major developmental stages of Drosophila melanogaster...
Show moreAging is a biological process that has many detrimental effects due to the accumulation of oxidative damage to key biomolecules due to the action of free radicals. Methionine sulfoxide reductase (Msr) functions to repair oxidative damage to methionine residues. Msr comes in two forms, MsrA and MsrB, each form has been shown to reduce a specific enantiomer of bound and free oxidized methionine. Effects of Msr have yet to be studied in the major developmental stages of Drosophila melanogaster despite the enzymes elevated expression during these stages. A developmental timeline was determined for MsrA mutant, MsrB mutant, and double null mutants against a wild type control. Results show that the Msr double mutant is delayed approximately 20 hours in the early/mid third instar stage while each of the single mutants showed no significant difference to the wild type. Data suggests that the reasoning of this phenomenon is due to an issue gaining mass.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00004200, http://purl.flvc.org/fau/fd/FA00004200
- Subject Headings
- Aging -- Molecular aspects, Cellular signal transduction, Drosophila melanogaster -- Genetics, Mitochondrial pathology, Mutation (Biology), Oxidative stress
- Format
- Document (PDF)
- Title
- Development of an Innovative Daily QA System for Pencil-Beam Scanning Proton Therapy.
- Creator
- Kassel, Maxwell, Shang, Charles, Muhammad, Wazir, Florida Atlantic University, Department of Physics, Charles E. Schmidt College of Science
- Abstract/Description
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In this work, we have developed a robust daily quality assurance (QA) system for pencil-beam scanning (PBS) dosimetry. A novel phantom and multi-PTV PBS plan were used in conjunction with the Sun Nuclear Daily QA3 multichamber detector array to verify output, range, and spot position. The sensitivity to detect change in these parameters with our designed tests was determined empirically. Associated tolerance levels were established based on these sensitivities and guidelines published in...
Show moreIn this work, we have developed a robust daily quality assurance (QA) system for pencil-beam scanning (PBS) dosimetry. A novel phantom and multi-PTV PBS plan were used in conjunction with the Sun Nuclear Daily QA3 multichamber detector array to verify output, range, and spot position. The sensitivity to detect change in these parameters with our designed tests was determined empirically. Associated tolerance levels were established based on these sensitivities and guidelines published in recent American Association of Physics in Medicine (AAPM) task group reports. The output has remained within the 3% tolerance and the range was within ±1mm. Spot position has remained within ±2mm. This daily QA procedure is quick and efficient with the time required for setup and delivery at less than 10 minutes.
Show less - Date Issued
- 2020
- PURL
- http://purl.flvc.org/fau/fd/FA00013623
- Subject Headings
- Proton Therapy, Radiation dosimetry, Quality assurance
- Format
- Document (PDF)
