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Pages
- Title
- Quantification and Analysis of Cancer-Cell Derived Exosomes.
- Creator
- Urdaneta, Alexandra, Iragavarapu-Charyulu, Vijaya, Keating, Patricia
- Abstract/Description
-
Over 90% of cancer-related deaths are due to metastasis. Tumor-cell derived extracellular vesicles or exosomes are thought to contribute to metastasis. However, there is no standardized method to isolate exosomes. We hypothesize that polymer-based kits, in particular ExoQuick-TC (EQTC), may be better for isolating exosomes when compared to ultracentrifugation. In this study, we used three different methods to isolate exosomes from 4T1 murine mammary tumor cells. Samples isolated through...
Show moreOver 90% of cancer-related deaths are due to metastasis. Tumor-cell derived extracellular vesicles or exosomes are thought to contribute to metastasis. However, there is no standardized method to isolate exosomes. We hypothesize that polymer-based kits, in particular ExoQuick-TC (EQTC), may be better for isolating exosomes when compared to ultracentrifugation. In this study, we used three different methods to isolate exosomes from 4T1 murine mammary tumor cells. Samples isolated through ultracentrifugation (UC), EQTC, and Total Exosome Isolation Reagent (TEIR) were analyzed to assess quantity and quality of exosomes by nanoparticle tracking analysis (NTA) and flow cytometry (FC). Using NTA, we found that each method yielded samples with varying average concentration and particle size. FC analysis revealed UC to be the most effective method in yielding a high number of verified exosomes. Standardizing the isolation method and assessment will help in determining the role of exosomes in cancer metastasis.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FAU_SR00000055
- Subject Headings
- College students --Research --United States.
- Format
- Document (PDF)
- Title
- Trabecular Structure through Ontogeny in the Florida Manatee (Trichechus manatus latirostris).
- Creator
- Lindsey, Laura, Ingle, Danielle, Porter, Marianne
- Abstract/Description
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Manatees, who use their vertebral column to propel themselves in swimming, are the product of a major evolutionary shift from land to water. This project explores the structure of trabecular (spongy) bone, which changes with force direction and magnitude, from the vertebral column of manatees. The goal of this research is to investigate the structural properties of manatee vertebral trabecular bone to better understand this animal's development and swimming mechanics. Vertebrae were dissected...
Show moreManatees, who use their vertebral column to propel themselves in swimming, are the product of a major evolutionary shift from land to water. This project explores the structure of trabecular (spongy) bone, which changes with force direction and magnitude, from the vertebral column of manatees. The goal of this research is to investigate the structural properties of manatee vertebral trabecular bone to better understand this animal's development and swimming mechanics. Vertebrae were dissected from four regions of the vertebral column and scanned with micro-computed tomography. Images were analyzed in BoneJ to quantify trabecular width, number, length, bone volume fraction (amount of bone/total area) and degree of anisotropy (orientation bias). Results from this project will be paired with mechanical data in future work to better understand forces on the vertebral column in a swimming mammal throughout development, and how these properties may have diverged from those found in their terrestrial counterparts.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FAU_SR00000043
- Subject Headings
- College students --Research --United States.
- Format
- Document (PDF)
- Title
- Understanding Sport-Related Concussions.
- Creator
- Coviello, Kassi, Nasrallah, Joseph, Escaleras, Monica, Levy, Eric
- Abstract/Description
-
In 2012, the amount of reported concussions doubled in the past ten years to 3.8 million. Concussions and head-related injuries caused by contact sports can lead to long term traumatic brain injuries. With the increasing presence of these injuries, the National Football League and the National Hockey League have implemented extensive concussion protocols. This questions the underlying uncertainty of the future of contact sports. Our project addresses the question of hypothetically, if you had...
Show moreIn 2012, the amount of reported concussions doubled in the past ten years to 3.8 million. Concussions and head-related injuries caused by contact sports can lead to long term traumatic brain injuries. With the increasing presence of these injuries, the National Football League and the National Hockey League have implemented extensive concussion protocols. This questions the underlying uncertainty of the future of contact sports. Our project addresses the question of hypothetically, if you had children, knowing what we know today about concussions/head related injuries, would you allow them to play contact sports. The decision to allow your child to play contact sports was motivated by the gender and age gap. The different levels of education had a statistically significant impact on the knowledge surrounding the current professional football and hockey concussion protocols.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FAU_SR00000031
- Subject Headings
- College students --Research --United States.
- Format
- Document (PDF)
- Title
- Society's Influence on Rape Culture.
- Creator
- Tran, Annalisa, Carreño-Lukasik, Gina
- Abstract/Description
-
In today's society, the investigations of the causes and patterns of rape incidents tend to focus on the actions or qualities of the individual victim or perpetrator. This literature review will analyze how society as a whole plays a role in positively or negatively influencing rape culture. Currently, society's stereotypes and attitudes about rape negatively influence rape culture by promoting rape myths that put blame on the victim or excuse perpetrators for their actions. The media also...
Show moreIn today's society, the investigations of the causes and patterns of rape incidents tend to focus on the actions or qualities of the individual victim or perpetrator. This literature review will analyze how society as a whole plays a role in positively or negatively influencing rape culture. Currently, society's stereotypes and attitudes about rape negatively influence rape culture by promoting rape myths that put blame on the victim or excuse perpetrators for their actions. The media also plays a significant role by promoting the romanticization of rape in pornography or the implicitly encouraging the action of rape in mainstream songs, books, etc. However, society has also influenced rape culture positively by creating social movements that raise awareness about rape and the solutions to combat it.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FAU_SR00000054
- Subject Headings
- College students --Research --United States.
- Format
- Document (PDF)
- Title
- Software Engineering: Social Impact and Perception.
- Creator
- Cardenas, Erika, Shorten, Connor, Escaleras, Monica
- Abstract/Description
-
New software technologies are rapidly changing the economy. These changes have presented problems such as job displacement, high barrier to entry, and a gender gap in the engineering communities. In order to see the views of Americans regarding the challenges of software technologies, we conducted an online survey, gathering 500 responses. In recent news stories, it has been shown that there is a gender gap in the tech industry, but the women that participated in our survey are interested in...
Show moreNew software technologies are rapidly changing the economy. These changes have presented problems such as job displacement, high barrier to entry, and a gender gap in the engineering communities. In order to see the views of Americans regarding the challenges of software technologies, we conducted an online survey, gathering 500 responses. In recent news stories, it has been shown that there is a gender gap in the tech industry, but the women that participated in our survey are interested in learning software engineering as much as men. Additionally, our research found that younger people are not only required to use software tools more frequently but are the most interested in learning how to build them. Finally, we found that a majority of people do not have any experience developing software. Our survey highlights some of the challenges of software technologies in the economy.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FAU_SR00000030
- Subject Headings
- College students --Research --United States.
- Format
- Document (PDF)
- Title
- Volitional Swimming Kinematics of the Black Tip Shark (Carcharhinus limbatus).
- Creator
- Nelski, Madison, Ruddy, B. T., Porter, Marianne
- Abstract/Description
-
Quantifying swimming kinematics of fishes often occurs in a lab setting using flumes, water treadmills, to examine movement. These methods rely on researchers to pick the animals swimming speed. We have been focusing on volitional kinematics in the lab where we quantify swimming as determined by the fish. However, our volitional swimming experiments are still limited to the space available in a lab setting. In this study, we examine swimming kinematics of black tip sharks (Carcharhinus...
Show moreQuantifying swimming kinematics of fishes often occurs in a lab setting using flumes, water treadmills, to examine movement. These methods rely on researchers to pick the animals swimming speed. We have been focusing on volitional kinematics in the lab where we quantify swimming as determined by the fish. However, our volitional swimming experiments are still limited to the space available in a lab setting. In this study, we examine swimming kinematics of black tip sharks (Carcharhinus limbatus) during their annual winter aggregations in South Florida. Using an aerial drone, video of sharks can be obtained through noninvasive methods in the wild, and examined frame-by-frame using the Loggerpro software. We track points along the shark’s midline to examine body curvature, tailbeat frequency, tailbeat amplitude, and whole-body swimming velocity. These data represent the first time we have been able to quantify kinematics in a free-swimming shark in the wild.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FAU_SR00000046
- Subject Headings
- College students --Research --United States.
- Format
- Document (PDF)
- Title
- Diet and Temporal Partitioning Facilitates Coexistence Between Two Octopus Species in a Shallow Coastal Habitat.
- Creator
- Bartz, Danielle, Bennice, Chelsea, Brooks, W. Randy, Office of Undergraduate Research and Inquiry
- Abstract/Description
-
Niche partitioning mechanisms may be used by closely related, sympatric species to reduce competition. We examine two dimensions of niche partitioning (diet and temporal) amongst the common octopus (Octopus vulgaris) and the Atlantic longarm octopus (Macrotritopus defilippi), which spatially overlap in a South Florida intracoastal habitat. SCUBA is used to collect octopus prey remains and gather supplemental feeding images to determine diets of each species. A 24h octopus monitoring camera...
Show moreNiche partitioning mechanisms may be used by closely related, sympatric species to reduce competition. We examine two dimensions of niche partitioning (diet and temporal) amongst the common octopus (Octopus vulgaris) and the Atlantic longarm octopus (Macrotritopus defilippi), which spatially overlap in a South Florida intracoastal habitat. SCUBA is used to collect octopus prey remains and gather supplemental feeding images to determine diets of each species. A 24h octopus monitoring camera records foraging activity times for each species. The common octopus consumes bivalves (49%), gastropods (32%) and crustaceans (19%), and forages at dawn, dusk, and nocturnal hours. The Atlantic longarm octopus consumes crustaceans (89%) and bivalves (11%), and forages during diurnal hours. Results suggest there is diet overlap between species and octopuses use temporal partitioning. This study provides additional findings to cephalopod niche partitioning literature, novel information on the ecology of the Atlantic longarm octopus, and conservation requirements for sand-dwelling species.
Show less - Date Issued
- 2017
- PURL
- http://purl.flvc.org/fau/fd/FA00005613
- Subject Headings
- College students --Research --United States.
- Format
- Document (PDF)
- Title
- American Sentiments on Behavioral & Cognitive Qualifications within Elected Officials.
- Creator
- Lopez Pelaez, Kevin, Escaleras, Monica
- Abstract/Description
-
This study examines Americans' attitudes toward the likeliness of public officials' conduct to be a factor in vote choice. Correspondingly, by expanding the umbrella of Political Psychology to explore perceptions of the behavioral sciences - this study also examines Americans' viewpoint for the disqualification of public officials due to cognitive decline.
- PURL
- http://purl.flvc.org/fau/fd/fauir000386
- Format
- Document (PDF)
