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Title
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Neuron- Specific Requirement of Autophagy Gene atg-18 for Lifespan and Dauer Morphogenesis of daf-2 Mutant C. elegans.
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Creator
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Phillips, Aileen, Jia, Kailiang, Harriet L. Wilkes Honors College
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Abstract/Description
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We recently discovered that autophagy, a conserved lysosomal degradation pathway, is necessary for increased lifespan and dauer morphogenesis of daf-2 mutant Caenorhabditis elegans. daf-2 encodes the worm orthologue of an insulin-like growth factor receptor. Moreover, we found neuronal autophagy activity is sufficient to fulfill this requirement. In this study we used the unc-42 promoter to express autophagy gene atg-18 in a subset of C. elegans neurons to examine whether autophagy activity...
Show moreWe recently discovered that autophagy, a conserved lysosomal degradation pathway, is necessary for increased lifespan and dauer morphogenesis of daf-2 mutant Caenorhabditis elegans. daf-2 encodes the worm orthologue of an insulin-like growth factor receptor. Moreover, we found neuronal autophagy activity is sufficient to fulfill this requirement. In this study we used the unc-42 promoter to express autophagy gene atg-18 in a subset of C. elegans neurons to examine whether autophagy activity in these neurons is sufficient to execute its function in extension of lifespan and completion of dauer morphogenesis in daf-2 mutants. Here we show expression of atg-18 in these ons fails to rescue the effect of atg-18 mutations on the longevity and dauer morphogenesis of daf-2 mutant worms, indicating that the requirement of neuronal autophagy in C. elegans for these effects is specific to neurons where unc-42 promoter is not active.
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Date Issued
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2015
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PURL
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http://purl.flvc.org/fau/fd/FA00003652
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Format
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Document (PDF)
