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- Title
- ESTABLISHING A SPONGE HYBRIDOMA FOR THE IN VITRO PRODUCTION OF A MARINE NATURAL PRODUCT.
- Creator
- Dougan, Cassady, Pomponi, Shirley A., Florida Atlantic University, Department of Marine Science and Oceanography, Charles E. Schmidt College of Science
- Abstract/Description
-
Marine sponges are one of the most prolific sources of chemical compounds with pharmaceutical importance. To establish a supply of such compounds large enough for clinical development, in vitro production methodology was investigated. Since all sponge cells do not divide in culture, it was hypothesized that the fusion of rapidly dividing cells of a sponge that does not produce any compounds of interest with cells of a nondividing but compound-producing sponge would result in a hybridoma that...
Show moreMarine sponges are one of the most prolific sources of chemical compounds with pharmaceutical importance. To establish a supply of such compounds large enough for clinical development, in vitro production methodology was investigated. Since all sponge cells do not divide in culture, it was hypothesized that the fusion of rapidly dividing cells of a sponge that does not produce any compounds of interest with cells of a nondividing but compound-producing sponge would result in a hybridoma that produces the compound of interest. In this study, hybridomas have been produced with cells of two marine sponges, Axinella corrugata, which produces the antitumor compound stevensine, and Geodia neptuni, which divides rapidly in a nutrient medium optimized for sponge cell culture. Successful hybridization and subsequent cell division and in vitro stevensine production may unlock the potential for sustainable mass production of other sponge-derived compounds.
Show less - Date Issued
- 2022
- PURL
- http://purl.flvc.org/fau/fd/FA00014024
- Subject Headings
- Marine natural products, Sponges, Hybridomas, In vitro
- Format
- Document (PDF)
- Title
- EVALUATION OF 3-D CULTURE METHODS FOR MARINE SPONGES.
- Creator
- Urban-Gedamke, Elizabeth, Pomponi, Shirley A., Florida Atlantic University, Department of Marine Science and Oceanography, Charles E. Schmidt College of Science
- Abstract/Description
-
Marine sponges are economically and environmentally valuable, but restoration, commercial, and biomedical demands exceed what wild populations and aquaculture can provide. In vitro culture of sponge cells is a promising alternative, but has remained elusive until recent breakthroughs involving improved nutrient medium M1 in two-dimensional culture. The advantages of three-dimensional over two-dimensional cell culture have been increasingly recognized. Here we report the successful 21-day...
Show moreMarine sponges are economically and environmentally valuable, but restoration, commercial, and biomedical demands exceed what wild populations and aquaculture can provide. In vitro culture of sponge cells is a promising alternative, but has remained elusive until recent breakthroughs involving improved nutrient medium M1 in two-dimensional culture. The advantages of three-dimensional over two-dimensional cell culture have been increasingly recognized. Here we report the successful 21-day culture of cells from the marine sponge Geodia neptuni using multiple three-dimensional cell culture methods: FibraCel© disks, thin hydrogel layers, gel micro droplets, and spheroid cell culture. These methods performed comparably to two-dimensional control cultures, and each method offers advantages for restoration or in vitro applications using sponge cells. Further optimization of these methods may lead to the ability to culture fully functioning sponges from dissociated, cryopreserved cells, which will reduce the need for wild harvest of sponge tissues for commercial, restoration, and biomedical purposes.
Show less - Date Issued
- 2021
- PURL
- http://purl.flvc.org/fau/fd/FA00013726
- Subject Headings
- Sponges, Cell Culture--methods
- Format
- Document (PDF)
- Title
- Effects of stressors on differential gene expression and secondary metabolites by Axinella corrugata.
- Creator
- Grima, Jennifer., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Sponges are an important source of bioactive marine natural products, or secondary metabolites. The common Caribbean reef sponge, Axinella corrugata, produces an antitumor and antibacterial chemical, stevensine. This study determined whether environmental stressors, such as elevated temperature and exposure to Amphibalanus amphitrite larvae, affect the production of stevensine by A.corrugata and if the stressors caused A.corrugata to exhibit differential gene expression. Temperature stress...
Show moreSponges are an important source of bioactive marine natural products, or secondary metabolites. The common Caribbean reef sponge, Axinella corrugata, produces an antitumor and antibacterial chemical, stevensine. This study determined whether environmental stressors, such as elevated temperature and exposure to Amphibalanus amphitrite larvae, affect the production of stevensine by A.corrugata and if the stressors caused A.corrugata to exhibit differential gene expression. Temperature stress resulted in no significant change in the production of stevensine; only two genes were significantly differentially expressed, including hsp70. Larval stressed resulted in increased production of stevensine and significant differential gene expression (more than seventy genes). This study suggests that A.corrugata may be resilient to elevations in temperature and that one of stevensine's roles in nature is as an antifoulant.
Show less - Date Issued
- 2013
- PURL
- http://purl.flvc.org/fcla/dt/3360781
- Subject Headings
- Axinellida, Sponges, Marine pharmacology, Adaptation (Biology), Gene expression, Genetic regulation, Stress (Physiology), Ecophysiology
- Format
- Document (PDF)
- Title
- An extraction optimization and determination of the absolute configuartion of clathric acid.
- Creator
- Rueda de Leon, Rolando, Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
Current research in natural products has heavily focused on the identification of potent biologically active compounds, specifically for drug development. The project detailed in this thesis focuses on the extraction of compounds from marine invertebrates as well as defining the absolute configuration for a compound. Utilizing marine invertebrates, the sonications method developed in this thesis provides an alternative approach to rapidly extract compounds for primary screening. This method...
Show moreCurrent research in natural products has heavily focused on the identification of potent biologically active compounds, specifically for drug development. The project detailed in this thesis focuses on the extraction of compounds from marine invertebrates as well as defining the absolute configuration for a compound. Utilizing marine invertebrates, the sonications method developed in this thesis provides an alternative approach to rapidly extract compounds for primary screening. This method is viable compared to a traditional overnight extraction method, without suffering compound degredation... Previously, clathric acid was isolated from an unknown Clathria sp. This compound is a bibyblic C-21 terpenoid shown to have mild antimicrobial activity against gram positive bacteria. With only its relative configuration established, additional amounts of clathric acid were required to define the overall absolute configuration. Identifying the Clathria sp. to be Clathria compressa, through spicule analysis, additional sponge tissues were then collected off the coast of Boca Raton, Florida to isolate additional quatities of clathric acid. The absolulte configuration was determined through circular dichroism and the octant rule to establish a final configuration for clathric acid's four carbon stereocenters to be: (3S, 7S, 8R, and 12S).
Show less - Date Issued
- 2012
- PURL
- http://purl.flvc.org/FAU/3355874
- Subject Headings
- Organic compounds, Analysis, Extraction (Chemistry), Natural products, Therapeutic use, Marine biotechology, Marine resources, Research, Sponges, Ecology
- Format
- Document (PDF)
- Title
- Novel compounds isolated from the marine sponge Clathria sp.
- Creator
- Rueda de Leon, Rolando, Gupta, Prasoon, West, Lyndon, Graduate College
- Date Issued
- 2011-04-08
- PURL
- http://purl.flvc.org/fcla/dt/3164772
- Subject Headings
- Sponges, Stereochemistry, Marine pharmacology
- Format
- Document (PDF)
- Title
- Leiodermatolide, a potent antimitotic macrolide from the marine sponge Leiodermatium sp.
- Creator
- Paterson, Ian, Dalby, S. M., Roberts, J. C., Naylor, G. J., Guzman, Esther A., Isbrucker, Richard A., Pitts, Tara P., Linley, P. A., Divlianska, D. B., Reed, John K., Wright, Amy E.
- Date Issued
- 2011
- PURL
- http://purl.flvc.org/fau/fd/FA00007465
- Subject Headings
- Antimitotic Agents, Macrolides, Sponges, Molecular structure, Nuclear Magnetic Resonance, Biomolecular, Cell lines
- Format
- Document (PDF)
- Title
- Population dynamics of Vibrio spp. associated with marine sponge microcosms.
- Creator
- Hoffmann, M., Fischer, M., Ottesen, A., McCarthy, Peter J., Lopez, Jose V., Brown, E. W., Monday, S. R.
- Date Issued
- 2010
- PURL
- http://purl.flvc.org/FCLA/DT/3351984
- Subject Headings
- Sponges, Vibrio, Microbial ecology, RNA, Ribosomal, 16S
- Format
- Document (PDF)
- Title
- The Lithistida: important sources of compounds useful in biomedical research.
- Creator
- Wright, Amy E.
- Date Issued
- 2010
- PURL
- http://purl.flvc.org/FCLA/DT/3331940
- Subject Headings
- Sponges, Biomedical research, Marine natural products, Bioactive compounds
- Format
- Document (PDF)
- Title
- Comparison of the anaerobic microbiota of deep-water Geodia spp. and sandy sediments in the Straits of Florida.
- Creator
- Bruck, W. M., Bruck, T. B., Self, W. T., Reed, John K., Nitecki, S. S., McCarthy, Peter J.
- Date Issued
- 2010
- PURL
- http://purl.flvc.org/FCLA/DT/3351982
- Subject Headings
- Microbial ecology, Marine sediments, Florida, Straits of, Fluorescence in situ hybridization, Sponges
- Format
- Document (PDF)
- Title
- Isolation and structure elucidation of novel compounds from marine cyanobacteria.
- Creator
- Meickle, Theresa, Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
The work of this dissertation examined the secondary metabolites of several blooms of the marine cyanobacterium Lyngbya collected in Guam and Florida with an emphasis on the isolation and structure elucidation of novel biologically active compounds. The introduction in Chapter One provides a brief history of marine natural products, a description of cyanobacteria and a summary of peptides isolated from Lyngbya collected in the Caribbean. In Chapter Two, a bioassay-guided fractionation of a...
Show moreThe work of this dissertation examined the secondary metabolites of several blooms of the marine cyanobacterium Lyngbya collected in Guam and Florida with an emphasis on the isolation and structure elucidation of novel biologically active compounds. The introduction in Chapter One provides a brief history of marine natural products, a description of cyanobacteria and a summary of peptides isolated from Lyngbya collected in the Caribbean. In Chapter Two, a bioassay-guided fractionation of a Floridian collection of Lyngbya polychroa led to the isolation and structural determination of the cytotoxin desacetylmicrocolin B and the known compounds microcolins A and B. The structures were established by nuclear magnetic resonance (NMR) spectroscopic analysis. All three compounds inhibited the growth of cancer cell lines HT-29 and IMR-32 at nanomolar concentrations. Microcolins A and B were found to have little activity in the ecological assay against the marine fungus Dendryphiella salina. Chapter Three describes the isolation and structure elucidation of the glycosidic, acyl proline derivative tumonoic acid J from a sample Lyngbya sp. collected in Guam. The planar structure was determined by 1D and 2D NMR spectroscopy in conjunction with high resolution-mass spectrometry (HR-MS) data. Tumonoic acid J showed moderate activity in the ecological assay against the marine fungus D. salina. In Chapter Four, NMR-guided fractionation of a Floridian sample of Lyngbya majuscula led to the isolation of two novel cyclic peptides porpoisamides A and B. The planar structures were determined by 1D and 2D NMR spectroscopy with HR-MS data. The absolute configurations of these two compounds were defined through chiral chromatographic methods and derivatization techniques., The porpoisamides showed only moderate activity in cytotoxicity assays against cancer cell lines HCT-116 and U2OS. Finally, Chapter Five examines a potential ecological role of compounds isolated from marine cyanobacte ria. These secondary metabolites may function as chemical defenses against competing microorganisms within marine environments. Compounds isolated from cyanobacteria were tested for anti-fungal activity against the saprophytic marine fungus D. salina. Three of the six compounds tested produced inhibitory activity at or below their natural concentration.
Show less - Date Issued
- 2010
- PURL
- http://purl.flvc.org/FAU/2978991
- Subject Headings
- Sponges, Ecology, Cyanobacteria, Biological control, Aquatic ecology
- Format
- Document (PDF)
- Title
- Therapeutic potential, mechanism of action, and ecology of novel marine natural products.
- Creator
- Winder, Priscilla L., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
The projects described in this dissertation are focused on compounds derived from the marine environment. Chapter 1 gives an introduction to the study of marine natural products to treat human ailments and a thorough review on compounds from lithistid sponges that have been isolated or synthesized since 2000. Chapter 2 describes the isolation and structure elucidation of two sesquiterpene substituted benzoquinone derivatives, petrosiquinones A and B, from a deep-water marine sponge from the...
Show moreThe projects described in this dissertation are focused on compounds derived from the marine environment. Chapter 1 gives an introduction to the study of marine natural products to treat human ailments and a thorough review on compounds from lithistid sponges that have been isolated or synthesized since 2000. Chapter 2 describes the isolation and structure elucidation of two sesquiterpene substituted benzoquinone derivatives, petrosiquinones A and B, from a deep-water marine sponge from the Family Petrosiidae. Although initially purified following activity in a (Sb(B-catenin/Tcf4 assay they were later followed using tumor cell line cytotoxicity assays. Petrosiquinone A was the more active of the two compounds with moderate cytotoxicity in the DLD-1, PANC-1, and AsPC-1 cell lines. In Chapter 3, the isolation and structure elucidation of two new marine-derived macrolides, madeirolide A and B, isolated from a deep-water lithistid sponge of the genus Leiodermatium is described., They were isolated using numerous chromatographic techniques and the structures were elucidated on the basis of 1D and 2D NMR spectra coupled with high resolution-mass spectrometry (HR-MS) data. Madeirolide A and B inhibited the growth of the fungal pathogen Candida albicans with minimum inhibitory concentrations (MIC) of 12.5 and 25 (So(Bg/mL, respectively, but were not cytotoxic in tumor cell assays under the conditions tested. Chapter 4 describes work performed to determine the molecular target of lasonolide A using affinity chromatography. The target of lasonolide A is of interest since lasonolide A is known to kill cancer cells in vitro through a unique mechanism., This chapter highlights the research performed to create an affinity matrix with immobilized lasonolide. A target has not been confirmed but there are a number of interesting hits that are being pursued. In Chapter 5, a liquid chromatography-mass spectrometry (LC-MS) screening method was established in order to rapidly identify the metabolites from numerous collections of Lyngbya spp. obtained from Broward and Lee County, Florida sites that may help marine ecologists assess the effects of Lyngbya spp. blooms on the environment. A link between the metabolites produced and nutrients from both the algal tissue and water column was also explored.
Show less - Date Issued
- 2009
- PURL
- http://purl.flvc.org/FAU/369392
- Subject Headings
- Natural products, Therapeutic use, Sponges, Ecology, Marine resources, Research, Marine biotechnology
- Format
- Document (PDF)
- Title
- Comparison of bacterial diversity within the coral reef sponge, Axinella corrugata, the encrusting coral Erythropodium caribaeorum.
- Creator
- Lopez, Jose V., Ranzer, L. K., Ledger, Angela, Schoch, B., Duckworth, Alan R., McCarthy, Peter J., Kerr, Russell G.
- Date Issued
- 2008
- PURL
- http://purl.flvc.org/fau/fd/FA00007036
- Subject Headings
- Sponges, Corals, Heterotrophic bacteria, Symbiosis, RNA, Ribosomal, 16S
- Format
- Document (PDF)
- Title
- Identification of the bacterial symbiont Entotheonella sp. in the mesohyl of the marine sponge Discodermia sp.
- Creator
- Bruck, W. M., Sennett, Susan H., Pomponi, Shirley A., Willenz, P., McCarthy, Peter J.
- Date Issued
- 2008
- PURL
- http://purl.flvc.org/FCLA/DT/3183806
- Subject Headings
- Bacteria Ecology, Symbiosis, Sponges
- Format
- Document (PDF)
- Title
- Total synthesis of a potent hybrid of the anticancer natural products dictyostatin and discodermolide.
- Creator
- Paterson, Ian, Naylor, G. J., Wright, Amy E.
- Date Issued
- 2008
- PURL
- http://purl.flvc.org/fau/fd/FA00007062
- Subject Headings
- Antineoplastic agents, Macrolides, Polyketides, Tubulin Modulators, Marine natural products, Sponges
- Format
- Document (PDF)
- Title
- Neopeltolide a new macrolide from a lithistid sponge of the family Neopeltidae.
- Creator
- Wright, Amy E., Botelho, J. C., Guzman, Esther A., Harmody, Dedra K., Linley, P. A., McCarthy, Peter J., Pitts, Tara P., Pomponi, Shirley A., Reed, John K.
- Date Issued
- 2007
- PURL
- http://purl.flvc.org/FCLA/DT/3333011
- Subject Headings
- Sponges, Macrolides, Marine natural products
- Format
- Document (PDF)
- Title
- Shifts in coral overgrowth by sponges and algae.
- Creator
- Lapointe, Brian E., Bedford, Bradley J., Littler, Mark M., Littler, Diane S.
- Date Issued
- 2007
- PURL
- http://purl.flvc.org/FCLA/DT/2875931
- Subject Headings
- Corals, Coral reefs and islands, Sponges, Algae, Salinity
- Format
- Document (PDF)
- Title
- Tsukamurella spongiae sp. nov., a novel actinomycete isolated from a deep-water marinesponge.
- Creator
- Olson, Julie B., Harmody, Dedra K., Bej, A. K., McCarthy, Peter J.
- Date Issued
- 2007
- PURL
- http://purl.flvc.org/fau/fd/FA00007117
- Subject Headings
- Curaçao, Sponges, Actinobacteria, Actinomycetes, RNA, Ribosomal, 16S
- Format
- Document (PDF)
- Title
- Biology of the Porifera: cell culture.
- Creator
- Pomponi, Shirley A.
- Date Issued
- 2006
- PURL
- http://purl.flvc.org/FCLA/DT/3342232
- Subject Headings
- Porifera, Sponges, Cell culture, Biology
- Format
- Document (PDF)
- Title
- Chemistry and biology of the discodermolides, potent mitotic spindle poisons.
- Creator
- Gunasekera, Sarath P., Wright, Amy E.
- Date Issued
- 2005
- PURL
- http://purl.flvc.org/FCLA/DT/3352941
- Subject Headings
- Polyketides, Sponges, Immunosuppressive agents, Antimitotic agents, Chemistry, Biology
- Format
- Document (PDF)
- Title
- Associated bacterial communities of two deep-water sponges.
- Creator
- Olson, Julie B., McCarthy, Peter J.
- Date Issued
- 2005
- PURL
- http://purl.flvc.org/FCLA/DT/3350900
- Subject Headings
- Sponges, Gel electrophoresis, Heterotrophic bacteria, Host-bacteria relationships
- Format
- Document (PDF)