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Title: Effects of GUS Gene Integration in Tobacco Plants.
Creator: Cruz, Laura, Zhang, Xing-Hai
Abstract/Description: The β-glucuronidase (GUS) gene was isolated in 1986 from the bacterium Escherichia coli. Since then it has been widely used as a reporter gene in genetically modified organisms serving to study gene expression and tissue specificity of different promoter sequences. We have introduced the GUS gene into tobacco plants through Agrobacterium-mediated genomic transformation. The plants that were confirmed to be expressing the GUS gene were grown to propagate a new (T1) generation. The T1 plants...
Show moreThe β-glucuronidase (GUS) gene was isolated in 1986 from the bacterium Escherichia coli. Since then it has been widely used as a reporter gene in genetically modified organisms serving to study gene expression and tissue specificity of different promoter sequences. We have introduced the GUS gene into tobacco plants through Agrobacterium-mediated genomic transformation. The plants that were confirmed to be expressing the GUS gene were grown to propagate a new (T1) generation. The T1 plants were analyzed for tissue specificity of GUS expression. The results to date seem to indicate that there is some variation in GUS expression between plant lines. The mechanisms of GUS gene integration in the plant genome as well as the possible effects it can have on a plant’s genomic structure are assessed.
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Date Issued: 2014
PURL: http://purl.flvc.org/fau/fd/FA0005009
Subject Headings: College students --Research --United States.
Format: Document (PDF)

Title: Diagnosis of Citrus Greening Disease by qPCR Analysis.
Creator: Rocha, Fernando, Zhang, Xing-Hai
Abstract/Description: Citrus greening, also known as Huanglongbing disease, is a phloem restrictive disease that affects orange as well as other citrus trees. The disease is caused by the gram negative bacteria Candidatus Liberibacter asiaticus. The bacteria is transmitted by the Asian psyllid, Diaphorina citri. The bacteria causes the tree to produce small and bitter oranges, the roots shrink and the leaves molt. There is currently no cure for this disease. The best way to manage citrus greening is by removing...
Show moreCitrus greening, also known as Huanglongbing disease, is a phloem restrictive disease that affects orange as well as other citrus trees. The disease is caused by the gram negative bacteria Candidatus Liberibacter asiaticus. The bacteria is transmitted by the Asian psyllid, Diaphorina citri. The bacteria causes the tree to produce small and bitter oranges, the roots shrink and the leaves molt. There is currently no cure for this disease. The best way to manage citrus greening is by removing infected trees, implementing healthy planting material and controlling the psyllid population. Quantitative real time PCR (qPCR) was used to verify whether or not a given orange tree had citrus greening disease. DNA was extracted from leaves from eight trees. A qPCR analysis was performed using a primer with the bacteria DNA. Three trees were successfully diagnosed with citrus greening using this method.
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Date Issued: 2018
PURL: http://purl.flvc.org/fau/fd/FAU_SR00000051
Subject Headings: College students --Research --United States.
Format: Document (PDF)

Title: Isolation and Relative Configuration Determination of Two Pairs of Bioactive diastereomers from Cacospongia cf. linteiformis.
Creator: Zhang, Long, West, Lyndon, Graduate College
Abstract/Description: Cacospongia cf. linteiformis is a marine sponge collected from Bahamas possessing various bioactive compounds. Two pairs of diastereomers spongianolides E&F were isolated for the first time through function group modification acetylation, and their relative configurations were determined based on Nuclear Overhauser Effect Spectroscopy NOESY. The mixture of isomers of spongianolides E&F are exhibiting inhibition against Schnurri-3. Bioassays will be repeated to unveil the assignment of the...
Show moreCacospongia cf. linteiformis is a marine sponge collected from Bahamas possessing various bioactive compounds. Two pairs of diastereomers spongianolides E&F were isolated for the first time through function group modification acetylation, and their relative configurations were determined based on Nuclear Overhauser Effect Spectroscopy NOESY. The mixture of isomers of spongianolides E&F are exhibiting inhibition against Schnurri-3. Bioassays will be repeated to unveil the assignment of the bioactivities for the four isomers.
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Date Issued: 2014
PURL: http://purl.flvc.org/fau/fd/FA00005865
Format: Document (PDF)

Title: The role of serotonin 5-HT2A receptors in memory and cognition.
Creator: Zhang, Gongliang, Stackman, Robert W.
Date Issued: 2015-10-06
PURL: http://purl.flvc.org/fau/fd/FAUIR000190
Format: Citation

Title: The Relationship between Community Design and Crashes Involving Older Drivers and Pedestrians.
Creator: Dumbaugh, Eric, Zhang, Yi
Date Issued: 2013-03-02
PURL: http://purl.flvc.org/fau/flvc_fau_islandoraimporter_10.1177_0739456X12468771_1649948678
Format: Document (PDF)

Title: Coastal Sediment Reflectance Analysis using Hyperspectral Remote Sensing.
Creator: Selch, Donna, Zhang, Caiyun, Graduate College, Oleinik, Anton E.
Abstract/Description: Quantitative assessment of substrate classification for sand properties is needed for land management and conservation. Establishing a sand spectral library is the first step in this process. Hyperspectal analysis allows for rapid, nondestructive data acquisition. This process uses an ASD spectrometer in a laboratory setting with an artificial light source to collect the spectra. Sand collected worldwide was also analyzed for grain size and composition. Development of spectral libraries of...
Show moreQuantitative assessment of substrate classification for sand properties is needed for land management and conservation. Establishing a sand spectral library is the first step in this process. Hyperspectal analysis allows for rapid, nondestructive data acquisition. This process uses an ASD spectrometer in a laboratory setting with an artificial light source to collect the spectra. Sand collected worldwide was also analyzed for grain size and composition. Development of spectral libraries of sand is an essential factor to facilitate analytical techniques to monitor coastal problems including erosion and beach nourishment. This in turn can affect various flora and fauna which requires specific substrate to grow, nest, or live. Preliminary results show that each sand sample has a unique signature that can be identified using hyperspectral data.
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Date Issued: 2014
PURL: http://purl.flvc.org/fau/fd/FA00005166
Format: Document (PDF)

Title: Tissue-specific requirement of the autophagy gene atg-18 in controlling C. elegans dauer morphogenesis, fat metabolism and adult longevity.
Creator: Minnerly, Justin, Zhang, Jiuli, Graduate College, Jia, Kailiang
Abstract/Description: The conserved insulin growth factor IGF signaling pathway is one of the major regulators of lifespan in many species including C. elegans. In C. elegans the insulin/IGF-like receptor is encoded by the daf-2 gene, mutations in which result in lifespan extension, fat accumulation and dauer formation. The daf-2 activity in the nervous system controls these phenotypes cell non-autonomously. Interestingly, the longevity phenotype of daf-2 mutant worms is dependent on macroautophagy hereafter...
Show moreThe conserved insulin growth factor IGF signaling pathway is one of the major regulators of lifespan in many species including C. elegans. In C. elegans the insulin/IGF-like receptor is encoded by the daf-2 gene, mutations in which result in lifespan extension, fat accumulation and dauer formation. The daf-2 activity in the nervous system controls these phenotypes cell non-autonomously. Interestingly, the longevity phenotype of daf-2 mutant worms is dependent on macroautophagy hereafter autophagy. Autophagy is a highly conserved lysosomal degradation pathway involved in the removal of long-lived proteins and cytoplasmic organelles. During autophagy, cellular components are sequestered into the double-membrane autophagosomes and delivered to lysosomes for degradation. Increasing evidence has emerged that the autophagy process is a central regulator of lifespan that is required for the effects of DAF-2 signaling, dietary restriction and some mitochondrial mutations on C. elegans longevity. It is unknown however whether autophagy activity in every tissue or in a single tissue mediates the influence of these longevity signals. To address this question, we examined the tissue requirement of autophagy gene atg-18 for the lifespan of wild type animals and the daf-2 mutant. We discovered that neurons and intestinal cells are two key tissues where atg-18 mediates the effect of DAF-2 insulin-like signaling on lifespan, fat accumulation and dauer morphogenesis, suggesting autophagy acts cell non-autonomously in controlling C. elegans dauer formation, fat metabolism and adult longevity.
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Date Issued: 2014
PURL: http://purl.flvc.org/fau/fd/FA00005160
Format: Document (PDF)

Title: Neuronal autophagy activity is essential for insulin growth factor signaling-regulated lifespan extension in C. elegans.
Creator: Minnerly, Justin, Jia, Kailiang, Zhang, Jiuli, Graduate College
Abstract/Description: The conserved insulin growth factor IGF signaling pathway is one of the major regulators of lifespan in many species including C. elegans. In C. elegans the insulin/IGF-like receptor is encoded by the daf-2 gene, mutations in which result in lifespan extension. The daf-2 activity in the nervous system controls these phenotypes cell nonautonomously. Interestingly, the longevity phenotype of daf-2 mutant worms is dependent on macroautophagy hereafter autophagy. Autophagy is a highly conserved...
Show moreThe conserved insulin growth factor IGF signaling pathway is one of the major regulators of lifespan in many species including C. elegans. In C. elegans the insulin/IGF-like receptor is encoded by the daf-2 gene, mutations in which result in lifespan extension. The daf-2 activity in the nervous system controls these phenotypes cell nonautonomously. Interestingly, the longevity phenotype of daf-2 mutant worms is dependent on macroautophagy hereafter autophagy. Autophagy is a highly conserved lysosomal degradation pathway involved in the removal of long-lived proteins and cytoplasmic organelles. During autophagy, cellular components are sequestered into the double-membrane autophagosomes and delivered to lysosomes for degradation. Increasing evidence has emerged that the autophagy process is a central regulator of lifespan that is required for the effects of DAF-2 signaling, dietary restriction and some mitochondrial mutations on C. elegans longevity. It is unknown however whether autophagy activity in every tissue or in a single tissue mediates the influence of these longevity signals. To address this question, we examined the tissue requirement of the autophagy gene atg-18 for the lifespan of wild type animals and the daf-2 mutant. We discovered that neurons and intestinal cells are two key tissues where atg-18 mediates the effect of DAF-2 insulin-like signaling on lifespan, suggesting autophagy acts cell nonautonomously in controlling C. elegans adult longevity. Moreover, we found that neuronal release of neuropeptides is required for the cell non-autonomous function of neuronal autophagy activity in controlling C. elegans lifespan.
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Date Issued: 2015
PURL: http://purl.flvc.org/fau/fd/FA00005900
Format: Document (PDF)

Title: Individual Tree Segmentation from LiDAR Point Clouds for Urban Forest Inventory.
Creator: Zhang, Caiyun, Zhou, Yuhong, Qiu, Fang
Abstract/Description: The objective of this study is to develop new algorithms for automated urban forest inventory at the individual tree level using LiDAR point cloud data. LiDAR data contain three-dimensional structure information that can be used to estimate tree height, base height, crown depth, and crown diameter. This allows precision urban forest inventory down to individual trees. Unlike most of the published algorithms that detect individual trees from a LiDAR-derived raster surface, we worked directly...
Show moreThe objective of this study is to develop new algorithms for automated urban forest inventory at the individual tree level using LiDAR point cloud data. LiDAR data contain three-dimensional structure information that can be used to estimate tree height, base height, crown depth, and crown diameter. This allows precision urban forest inventory down to individual trees. Unlike most of the published algorithms that detect individual trees from a LiDAR-derived raster surface, we worked directly with the LiDAR point cloud data to separate individual trees and estimate tree metrics. Testing results in typical urban forests are encouraging. Future works will be oriented to synergize LiDAR data and optical imagery for urban tree characterization through data fusion techniques.
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Date Issued: 2015-06-16
PURL: http://purl.flvc.org/fau/fd/FAUIR000061
Format: Citation

Title: The cell non-autonomous function of ATG-18 is essential for neuroendocrine regulation of Caenorhabditis elegans lifespan.
Creator: Justin Minnerly, Jiuli Zhang, Thomas Parker, Tiffany Kaul, Kailiang Jia
Abstract/Description: Restriction of dietary food without malnutrition robustly extends lifespan in more than twenty species. It was also reported that fruit flies (Drosophila melanogaster) deficient in olfactory function live longer and that the longevity induced by food restriction is partially due to decreased olfaction. These observations suggest food assimilation through the gastrointestinal tract and food smell detected by olfactory neurons influence lifespan. The insulin growth factor signaling pathway is...
Show moreRestriction of dietary food without malnutrition robustly extends lifespan in more than twenty species. It was also reported that fruit flies (Drosophila melanogaster) deficient in olfactory function live longer and that the longevity induced by food restriction is partially due to decreased olfaction. These observations suggest food assimilation through the gastrointestinal tract and food smell detected by olfactory neurons influence lifespan. The insulin growth factor signaling pathway is regulated by nutrient levels and has been shown to mediate the lifespan extension conferred by food restriction and defective gustatory neurons in the nematode Caenorhabditis elegans. However, the mechanism remains unclear. Autophagy is a lysosomal degradation pathway and is sensitive to nutrient availability. We found autophagy activity in the intestine and food sensory neurons acts in parallel to mediate food restriction and insulin signaling regulated lifespan extension in Caenorhabditis elegans. Moreover, intestinal and neuronal autophagy converge on unidentified neurons to control the secretion of neuropeptides that regulate lifespan. These data suggest autophagy is an essential component in a neuroendocrine pathway that coordinates how environmental food cues detected by sensory neurons and food nutrients assimilated by the intestine influence lifespan. These findings may contribute to understanding the aging process in mammals.
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Date Issued: 2017
PURL: http://purl.flvc.org/fau/fd/FAUIR000531
Format: Document (PDF)

Title: Intestinal autophagy activity is essential for host defense against Salmonella typhimurium infection in Caenorhabditis elega.
Creator: Alexander Curt, Jiuli Zhang, Justin Minnerly, Kailiang Jia
Abstract/Description: Salmonella typhimurium infects both intestinal epithelial cells and macrophages. Autophagy is a lysosomal degradation pathway that is present in all eukaryotes. Autophagy has been reported to limit the Salmonella replication in Caenorhabditis elegans and in mammals. However, it is unknown whether intestinal autophagy activity plays a role in host defense against Salmonella infection in C. elegans. In this study, we inhibited the autophagy gene bec-1 in different C. elegans tissues and...
Show moreSalmonella typhimurium infects both intestinal epithelial cells and macrophages. Autophagy is a lysosomal degradation pathway that is present in all eukaryotes. Autophagy has been reported to limit the Salmonella replication in Caenorhabditis elegans and in mammals. However, it is unknown whether intestinal autophagy activity plays a role in host defense against Salmonella infection in C. elegans. In this study, we inhibited the autophagy gene bec-1 in different C. elegans tissues and examined the survival of these animals following Salmonella infection. Here we show that inhibition of the bec-1 gene in the intestine but not in other tissues confers susceptibility to Salmonella infection, which is consistent with recent studies in mice showing that autophagy is involved in clearance of Salmonella in the intestinal epithelial cells. Therefore, the intestinal autophagy activity is essential for host defense against Salmonella infection from C. elegans to mice, perhaps also in humans.
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Date Issued: 2014
PURL: http://purl.flvc.org/fau/fd/FAUIR000527
Format: Document (PDF)

Title: ECO-DRIVING STUDY ON TRUCKS ALONG A SIGNALIZED ARTERIAL WITH SIGNIFICANT FREIGHT TRAFFIC.
Creator: Xiao, Xiao, Zhang, Yunlong, Freight Mobility Research Institute
Abstract/Description: The project starts from a literature review of the topics from these aspects: the studies of emission models, the eco-driving applications for heavy-duty vehicles (Trucks), Eco-driving and signal control, the benefits from CAVs and Simulation using MOVES and VISSIM. The research on Multiclass/heterogeneous traffic modeling is also reviewed. To define the problem, the research starts with the analysis of the influence the truck percentage has on the individual signalized intersection and on a...
Show moreThe project starts from a literature review of the topics from these aspects: the studies of emission models, the eco-driving applications for heavy-duty vehicles (Trucks), Eco-driving and signal control, the benefits from CAVs and Simulation using MOVES and VISSIM. The research on Multiclass/heterogeneous traffic modeling is also reviewed. To define the problem, the research starts with the analysis of the influence the truck percentage has on the individual signalized intersection and on a coordinated signal corridor. The simulation results show the high percentage of heavy-duty vehicles in traffic may significantly degrade the signal control based on the concept of delay optimization mainly considering passenger cars. To solve the problem, an eco-driving strategy for freight mobility control at signalized intersections is introduced. It is by optimizing the travel time while maintaining optimal fuel consumptions and emissions. A two-level dynamic optimization is formulated. An emission weighted optimization is used to simulate vehicles passing the intersection with balanced travel time and emissions savings and compared to a baseline simulation without eco-driving consideration. A jerk penalty is added to ensure safety and comfort. Heavy-Duty Vehicles (HDVs) are the focus of this modeling effort. The emission term in the optimization used an instantaneous speed-acceleration based microscopic fuel consumption models and the results were validated by EPA's MOtor Vehicle Emission Simulator (MOVES) model. The results from this study showed that the weighting factor of the emission term in the objective function reaches an optimal at 0.5. Generally, the proposed method provided dynamic trajectories with slightly longer travel time than the baseline but reduce the emission at about 4% for Nitrogen oxide (NOx) and 7% for carbon dioxide (CO2) for different initial conditions (different distance approaching intersection). Based on the results, an optimal weighting factor of emission term and the range of distances to apply the eco-driving strategy are recommended. A case study is performed to simulate the recommended model, with varying HDV percentages. The test results showed an overall emission reduction of 6% for NOx and 6% for CO2 according to MOVES. To show the relationship between truck percentage and discharge rate, a multiply linear regression is conducted, and the results are shown in the appendix. The data in MOVES and the emission models used are also presented in the appendix.
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Date Issued: 2018
PURL: http://purl.flvc.org/fau/fd/FAUIR000428
Subject Headings: Freight Research, Freight and freightage, Freight transportation
Format: Document (PDF)

Title: TRUCK PARKING STUDY: UNVEILING THE PARKING SPACE DENSITY AND TRUCK VOLUME RELATIONSHIP: PHASE 1.
Creator: Zhang, Yunlong, Wang, Bruce, Freight Mobility Research Institute
Abstract/Description: Truck parking problem has been a national concern for many years due to the lack of parking space along the major freight corridors. How to assess the parking capacity adequacy remains a major challenge in addressing the provision of parking space. This study aims to reveal the relationship between the incoming truck volume and the parking capacity needed for the rest area under the hours-of-service (HOS) regulations through computer simulation.
Date Issued: 2018
PURL: http://purl.flvc.org/fau/fd/FAUIR000423
Subject Headings: Freight Research, Freight and freightage, Freight transportation
Format: Document (PDF)

Title: Analysis of the Urban Heat Island Effect in Shijiazhuang, China Using Satellite and Airborne Data.
Creator: Liu, Kai, Su, Hongbo, Zhang, Lifu, Yang, Hang, Zhang, Renhua, Li, Xueke
Abstract/Description: The urban heat island (UHI) effect resulting from rapid urbanization generally has a negative impact on urban residents. Shijiazhuang, the capital of Hebei Province in China, was selected to assess surface thermal patterns and its correlation with Land Cover Types (LCTs). This study was conducted using Landsat TM images on the mesoscale level and airborne hyperspectral thermal images on the microscale level. Land surface temperature (LST) was retrieved from four scenes of Landsat TM data in...
Show moreThe urban heat island (UHI) effect resulting from rapid urbanization generally has a negative impact on urban residents. Shijiazhuang, the capital of Hebei Province in China, was selected to assess surface thermal patterns and its correlation with Land Cover Types (LCTs). This study was conducted using Landsat TM images on the mesoscale level and airborne hyperspectral thermal images on the microscale level. Land surface temperature (LST) was retrieved from four scenes of Landsat TM data in the summer days to analyze the thermal spatial patterns and intensity of surface UHI (SUHI). Surface thermal characteristics were further examined by relating LST to percentage of imperious surface area (ISA%) and four remote sensing indices (RSIs), the Normalized Difference Vegetation Index (NDVI), Universal Pattern Decomposition method (VIUPD), Normalized Difference Built-up Index (NDBI) and Biophysical Composition Index (BCI). On the other hand, fives scenes of airborne TASI (Thermal Airborne Spectrographic Imager sensor) images were utilized to describe more detailed urban thermal characteristics of the downtown of Shijiazhuang city. Our results show that an obvious surface heat island effect existed in the study area during summer days, with a SUHI intensity of 2–4 °C. The analyses reveal that ISA% can provide an additional metric for the study of SUHI, yet its association with LST is not straightforward and this should a focus in future work. It was also found that two physically based indices, VIUPD and BCI, have the potential to account for the variation in urban LST. The results concerning on TASI indicate that diversity of impervious surfaces (rooftops, concrete, and mixed asphalt) contribute most to the SUHI, among all of the land cover features. Moreover, the effect of impervious surfaces on LST is complicated, and the composition and arrangement of land cover features may play an important role in determining the magnitude and intensity of SUHI. Overall, the analysis of urban thermal signatures at two spatial scales complement each other and the use of airborne imagery data with higher spatial resolution is helpful in revealing more details for understanding urban thermal environments.
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Date Issued: 2015-04-20
PURL: http://purl.flvc.org/fau/fd/FAUIR000054
Format: Citation

Title: NR2C in the Thalamic Reticular Nucleus; Effects of the NR2C Knockout.
Creator: Zhang, Yuchun, Buonanno, Andres, Vertes, Robert P., Hoover, Walter B., Lisman, John E., Zhang, Zhe
Abstract/Description: NMDAR antagonists can evoke delta frequency bursting in the nucleus reticularis of the thalamus (nRT). The mechanism of this oscillation was determined; antagonist blocks an NR2C-like conductance that has low Mg block at resting potential and thus can contribute a resting inward current in response to ambient glutamate. Block of this current hyperpolarizes the cell, deinactivating T-type Ca channels and thus triggering delta frequency bursting. The basis for assuming a NR2C-like conductance...
Show moreNMDAR antagonists can evoke delta frequency bursting in the nucleus reticularis of the thalamus (nRT). The mechanism of this oscillation was determined; antagonist blocks an NR2C-like conductance that has low Mg block at resting potential and thus can contribute a resting inward current in response to ambient glutamate. Block of this current hyperpolarizes the cell, deinactivating T-type Ca channels and thus triggering delta frequency bursting. The basis for assuming a NR2C-like conductance was that (1) transcripts for NR2C are abundant in the thalamus and (2) the current-voltage curve of the synaptically evoked NMDAR current has the low rectification characteristic of NR2C. In the current study, we have sought to determine whether the channels that generate the NMDAR current are NR2C-like or are actually comprised of receptors containing NR2C. We studied the current-voltage curve of synaptically evoked NMDAR current in the nRT of NR2C knockout mice. In wild-type mice, the current was weakly voltage dependent, as previously observed in rats. This weak rectification was absent in NR2C KO mice. In contrast, NR2C KO had no effect on the strongly rectifying NMDAR current in pyramidal cells of the prefrontal cortex. These results demonstrate that the low rectification normally observed in the nRT is due to NR2C.
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Date Issued: 2012-07-25
PURL: http://purl.flvc.org/fau/fd/FAUIR000089
Format: Citation

Title: Marine ornamental shrimp:status and prospects.
Creator: Lin, Junda, Zhang, Dong, Creswell, R. LeRoy, Harbor Branch Oceanographic Institute
Date Issued: 1999
PURL: http://purl.flvc.org/fau/fd/FA00007315
Subject Headings: Ornamental shrimps, Coral reef ecology
Format: Document (PDF)

Title: Ingestion rateand feeding behavior of the peppermint shrimp Lysmata wurdemanni on Artemia nauplii.
Creator: Zhang, Dong, Lin, Junda, Creswell, R. LeRoy, Harbor Branch Oceanographic Institute
Date Issued: 1998
PURL: http://purl.flvc.org/fau/fd/FA00007269
Subject Headings: Shrimps, Shrimps--Feeding and feeds, Water temperature, Artemia
Format: Document (PDF)

Title: Emotional Response during Human-Virtual Partner Interaction.
Creator: Zhang, Mengsen, Dumas, Guillaume, Kelso, J. A. Scott, Graduate College, Tognoli, Emmanuelle
Abstract/Description: Emotion and coordinated movement complimentarily depicts our social experiences. How is motion colored? This study investigates variations in emotional responses during social coordination. Subjects were instructed to coordinate their finger movement with a Virtual Partner (VP), whose homologous movement was displayed as a video on the computer screen. The partner was driven by the Haken-Kelso-Bunz equations, an empirically validated model that captures behavioral and social coordination. It...
Show moreEmotion and coordinated movement complimentarily depicts our social experiences. How is motion colored? This study investigates variations in emotional responses during social coordination. Subjects were instructed to coordinate their finger movement with a Virtual Partner (VP), whose homologous movement was displayed as a video on the computer screen. The partner was driven by the Haken-Kelso-Bunz equations, an empirically validated model that captures behavioral and social coordination. It has been shown that people perceive VP as an intentional human agent. In each of 80 trials, subjects coordinated for 8 sec inphase or antiphase with VP, and then rated the partner’s intention (cooperation -VP intend same coordination pattern as human-, or competition) and subjective response to a Turing test of partners’ humanness. VP cooperated for half of the time, and could change its intention in the middle of a trial. Skin potential response (SPR) quantified the intensity of emotional responses. After validating the SPR measurements, we compared emotional responses by coordination pattern, cooperative~competitiveness, and humanness attribution. Subjects experienced higher emotional responses when they believed that their partner was human. This was observed both during coordination (ANOVA, p=0.020), and during rating (p=0.012). Furthermore during the rating period, higher emotional responses were found for cooperative behavior (p=0.012), modulated by VP’s change of intention and coordination pattern. This study suggests that emotional responses are strongly influenced by features of the partner’s behavior associated with humanness, cooperation and change of intention. Implications for mental health (e.g. autism) and design of socially cooperative machines will be discussed.
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Date Issued: 2014
PURL: http://purl.flvc.org/fau/fd/FA00005866
Format: Document (PDF)

Title: Regulatory Pattern of PUN Promoter for Gene Expression.
Creator: Velez, Stephanie, Kirke, Justin, Zhang, Xing-Hai, Office of Undergraduate Research and Inquiry
Abstract/Description: The purpose of this research was to analyze the regulatory pattern of the PUN promoter in the expression of a marker gene, β-glucoronidase (GUS), within regenerated tobacco plants. The genes for neomycin phosphotransferase (NPT II) and GUS were included in the coding region of the Ti plasmid construct. The NPTII gene drove antibiotic resistance and was used to select and identify homozygous lines through the segregation of the progeny. Analysis through histochemical staining and genetic...
Show moreThe purpose of this research was to analyze the regulatory pattern of the PUN promoter in the expression of a marker gene, β-glucoronidase (GUS), within regenerated tobacco plants. The genes for neomycin phosphotransferase (NPT II) and GUS were included in the coding region of the Ti plasmid construct. The NPTII gene drove antibiotic resistance and was used to select and identify homozygous lines through the segregation of the progeny. Analysis through histochemical staining and genetic assays rendered putative transgenic lines that were cultivated for further assessment of progeny. First generation histochemical analysis of 14-day tissue formation resulted in no levels of expression for the GUS gene, which demonstrated that the flower-specific PUN promoter was not active in the leaf tissue. Further testing of gene activity throughout all stages of tissue formation for the first generation lines is required in order to assess regulatory pattern of the PUN promoter.
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Date Issued: 2016
PURL: http://purl.flvc.org/fau/fd/FA00005606
Subject Headings: College students --Research --United States.
Format: Document (PDF)

Title: Genetic Engineering of Tomato Plants Expressing β-Glucuronidasethrough Agrobacterium-mediated Transformation.
Creator: Justs, Adriana, Kaplan, Noah, Zhang, Xing-Hai, Office of Undergraduate Research and Inquiry
Abstract/Description: Advancements in biotechnology have allowed us to study genetics and plant physiology by engineering transgenic plants. For our research we transformed Micro-Tom, a tomato variety developed for use in genetic research, using Agrobacterium mediated transformation. Within a time span of fourteen weeks, we inserted two distinct plasmid constructs (pCAMBIA2301 and E1492). Plants have the unique ability to regenerate their tissue and we took advantage of this ability to regenerate the transgenic...
Show moreAdvancements in biotechnology have allowed us to study genetics and plant physiology by engineering transgenic plants. For our research we transformed Micro-Tom, a tomato variety developed for use in genetic research, using Agrobacterium mediated transformation. Within a time span of fourteen weeks, we inserted two distinct plasmid constructs (pCAMBIA2301 and E1492). Plants have the unique ability to regenerate their tissue and we took advantage of this ability to regenerate the transgenic plants with antibiotic selection. Approximately one third of the explants endured the infection process and fourteen of these survived in the presence of kanamycin. By the end of the fourteenth week, eleven out of our fourteen plantlets had fully developed roots but only four survived to maturity. After verification with PCR and qPCR, we found that we generated two transgenic plants. Here we describe all the methods and techniques used to achieve these compelling results.could be the potential cause of this neurodegenerative disease, will help elucidate the role of this amino acid in ALS.
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Date Issued: 2016
PURL: http://purl.flvc.org/fau/fd/FA00005578
Subject Headings: College students --Research --United States.
Format: Document (PDF)

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