Current Search: Wound healing. (x)
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- Title
- The expression and analysis of a lysine-rich wound-response protein in tomato plants.
- Creator
- Kaplan, Noah, Zhang, Xing-Hai, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
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Understanding the genetic regulation of the response to wounding and wound healing in fruiting plants is imperative to maintaining agricultural sustainability, preserving the quality of food supplies, and ensuring the economic viability of agriculture. Many genes are known to be induced by wounding, providing both structural repair and defense. The KED gene in tobacco (Nicotiana tabacum) has been shown to be induced by wounding. We have identified its homologue gene in tomato (Solanum...
Show moreUnderstanding the genetic regulation of the response to wounding and wound healing in fruiting plants is imperative to maintaining agricultural sustainability, preserving the quality of food supplies, and ensuring the economic viability of agriculture. Many genes are known to be induced by wounding, providing both structural repair and defense. The KED gene in tobacco (Nicotiana tabacum) has been shown to be induced by wounding. We have identified its homologue gene in tomato (Solanum lycopersicum) that we named SlKED. We have analyzed gene expression pattern of SlKED through tomato growth and development and in response to wounding as well as hormonal and inhibitor treatments. We found that the plant hormone ethylene played a major role in the expression of SlKED. To further identify evidence for physiological and transductional functions of KED and SlKED, the tobacco KED gene was introduced to tomato and overexpressed by the fruit tissue-active PUN1 promoter from pepper (Capsicum annuum,). The expression of this gene was compared to the expression of the native SlKED gene and other known wound response genes in both the wild-type and transgenic tomato plants. The upregulation of the native SlKED gene by wounding was significantly muted in the tobacco KED-expressing transgenic plants. The expression of other genes known to be associated with wound response transduction pathways was also altered. Our studies implicate the KED gene in defense mechanisms for mechanical stress in tomato plants.
Show less - Date Issued
- 2016
- PURL
- http://purl.flvc.org/fau/fd/FA00004773, http://purl.flvc.org/fau/fd/FA00004773
- Subject Headings
- Wound healing., Wounds and injuries--Genetic aspects., Plant gene expression., Plant genetic regulation., Nanostructured materials--Physiological effect., Biomedical engineering.
- Format
- Document (PDF)
- Title
- Engineering of tissue inhibitor of metalloproteinases mutants as potential therapeutics.
- Creator
- Nagase, Hideaki, Brew, Keith
- Abstract/Description
-
Matrix metalloproteinases (MMPs) play a central role in many biological processes such as development, morphogenesis and wound healing, but their unbalanced activities are implicated innumerous disease processes such as arthritis, cancer metastasis, atherosclerosis, nephritis and fibrosis. One of the key mechanisms to control MMP activities is inhibition by endogenous inhibitors called tissue inhibitors of metalloproteinases (TIMPs). This review highlights the structures and inhibition...
Show moreMatrix metalloproteinases (MMPs) play a central role in many biological processes such as development, morphogenesis and wound healing, but their unbalanced activities are implicated innumerous disease processes such as arthritis, cancer metastasis, atherosclerosis, nephritis and fibrosis. One of the key mechanisms to control MMP activities is inhibition by endogenous inhibitors called tissue inhibitors of metalloproteinases (TIMPs). This review highlights the structures and inhibition mechanism of TIMPs, the biological activities of TIMPs, the unique properties of TIMP-3, and the altered specificity towards MMPs achieved by mutagenesis. A potential therapeutic use of TIMP variants is discussed.
Show less - Date Issued
- 2002-04-02
- PURL
- http://purl.flvc.org/fcla/dt/3327266
- Subject Headings
- Gene Therapy --Methods, Genetic Engineering --methods, Protein Structure, Tertiary, Rheumatic Diseases --therapy, Tissue Inhibitor of Metalloproteinases, Wound Healing --physiology, Metalloproteinases --Inhibitors --Therapeutic use
- Format
- Document (PDF)