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(1 - 4 of 4)
- Title
- Novel Detection Techniques for Viable but Nonculturable Vibrio Vulnificus Cells in Response to Elevated Salinity.
- Creator
- McHenry, Brandon, McCarthy, Peter J., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Vibrio vulnificus is a marine pathogen of human health concern, capable of causing potentially fatal wound infections in a select group of the population. Previous studies have indicated this species’ strong negative correlation with salinity, not typically found above 30 ppt. This study assessed the ability of V. vulnificus to become Viable But Nonculturable in response to elevated salinity (35 ppt) as well as investigated novel methods for confirming their entrance into this state. Results...
Show moreVibrio vulnificus is a marine pathogen of human health concern, capable of causing potentially fatal wound infections in a select group of the population. Previous studies have indicated this species’ strong negative correlation with salinity, not typically found above 30 ppt. This study assessed the ability of V. vulnificus to become Viable But Nonculturable in response to elevated salinity (35 ppt) as well as investigated novel methods for confirming their entrance into this state. Results showed a complete loss of culturability in both Environmental and Clinical strains of this bacterium by 9 days after inoculation. Using a High Content Imager, it was determined that these pathogens were not dying (< 10%) in response to the treatment and were partially becoming cocci (≈35%). This study indicates the importance of understanding the impact environmental parameters have on this human pathogen, and what it means for reliably detecting them.
Show less - Date Issued
- 2019
- PURL
- http://purl.flvc.org/fau/fd/FA00013240
- Subject Headings
- Vibrio vulnificus, Pathogenic microorganisms--Detection, Salinity
- Format
- Document (PDF)
- Title
- Epidermal lesions and mortality caused by vibriosis in deep-sea Bahamian echinoids: a laboratory study.
- Creator
- Bauer, John C., Young, Craig M., Harbor Branch Oceanographic Institute
- Date Issued
- 2000
- PURL
- http://purl.flvc.org/FCLA/DT/3350883
- Subject Headings
- Echinoidea, Sea urchins, Vibrio alginolyticus, Bacterial diseases, Mortality, Spatangoida
- Format
- Document (PDF)
- Title
- Population dynamics of Vibrio spp. associated with marine sponge microcosms.
- Creator
- Hoffmann, M., Fischer, M., Ottesen, A., McCarthy, Peter J., Lopez, Jose V., Brown, E. W., Monday, S. R.
- Date Issued
- 2010
- PURL
- http://purl.flvc.org/FCLA/DT/3351984
- Subject Headings
- Sponges, Vibrio, Microbial ecology, RNA, Ribosomal, 16S
- Format
- Document (PDF)
- Title
- The Occurrence of Vibrio vulnificus, V. parahaemolyticus and V. cholerae in the Indian River Lagoon, Florida, with Implications for Human Health.
- Creator
- Barbarite, Gabrielle M., McCarthy, Peter J., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Vibrio bacteria are emerging pathogens responsible for 80,000 illnesses and 100 deaths in the United States each year. Infections are directly linked to the marine environment and are acquired by consuming contaminated seafood or exposing wounds during aquatic activities. Florida has the highest national incidence of vibriosis, with 20% of its cases reported from the Indian River Lagoon region, a popular recreation destination. This study utilized a combination of cultivation and molecular...
Show moreVibrio bacteria are emerging pathogens responsible for 80,000 illnesses and 100 deaths in the United States each year. Infections are directly linked to the marine environment and are acquired by consuming contaminated seafood or exposing wounds during aquatic activities. Florida has the highest national incidence of vibriosis, with 20% of its cases reported from the Indian River Lagoon region, a popular recreation destination. This study utilized a combination of cultivation and molecular techniques to investigate the local distribution of V. vulnificus, V. parahaemolyticus and V. cholerae in this local waterway. The targeted species were found in an array of samples which may facilitate their transmission to humans. Overall, these bacteria were abundant in estuarine sediments (Vp: 2,439 CFU/g, Vv: 303 CFU/g, Vc: 176 CFU/g), on the sharp edges of oyster shells (Vp: 82 CFU/cm, Vv: 102 CFU/cm, Vc: 41 CFU/cm), and in the water column (Vp: 3.78 CFU/ml, Vv: 5.51 CFU/ml, Vc: 2.46 CFU/ml). Vibrio also pose a hazard to recreational anglers as they were recovered from fish (Vp: 61%, Vv: 55%, Vc: 30%), live bait shrimp (Vp: 80%, Vv: 37%, Vc: 0%) and hooks (Vp: 32%, Vv: 18%, Vc: 0%). Additionally, a molecular analysis of the V. vulnificus virulence revealed that the local population was dominated by disease-causing (vcgC) strains, which may explain why wound-related infections are common in this region. Vibrio occurrence varied both spatially and temporally due to their relationship with salinity and temperature. These bacteria exhibited a strong negative correlation with salinity, being particularly abundant near freshwater discharge locations. Due to Florida’s year-round warm climate, these species were found to be permanent members of the local microbial community. Seasonal peaks in abundance occurred between August and October, a period which corresponds with the warmest water temperatures as well as frequent rainfall. Predictive models were constructed based on these parameters to provide a better understanding of how, when and where Vibrio spp. may be encountered by humans. This information is important for both water management and healthcare initiatives, with an overall goal of improving local recreational safety.
Show less - Date Issued
- 2016
- PURL
- http://purl.flvc.org/fau/fd/FA00004705
- Subject Headings
- Health risk assessment, Marine microbiology, Pathogenic microorganisms, Vibrio infections, Vibrionaceae
- Format
- Document (PDF)
