Current Search: Tissue Engineering (x)
View All Items
- Title
- DEVELOPMENT OF AN ELASTIC POLYMER-BASED DRUG DELIVERY SYSTEM FOR TISSUE REGENERATION.
- Creator
- Firoozi, Negar, Kang, Yunqing, Florida Atlantic University, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
In spite of the vast research on polymer-based tissue regeneration, extensive studies to develop an elastic and cell-promoting polymer biomaterial are still ongoing. However, using a renewable resource and a simple, environment-friendly synthesis route to synthesize an elastic polymer has not been successfully achieved yet. The objective of this work was to develop an elastic polymer for tissue engineering and drug delivery applications by using non-toxic, inexpensive and renewable monomers....
Show moreIn spite of the vast research on polymer-based tissue regeneration, extensive studies to develop an elastic and cell-promoting polymer biomaterial are still ongoing. However, using a renewable resource and a simple, environment-friendly synthesis route to synthesize an elastic polymer has not been successfully achieved yet. The objective of this work was to develop an elastic polymer for tissue engineering and drug delivery applications by using non-toxic, inexpensive and renewable monomers. A new nature-derived renewable material, xylitol, was used to synthesize an elastic polymer with the presence of a crosslinking agent, dodecanedioic acid. Here a simple melt condensation polymerization method was used to synthesize the poly(xylitoldodecanedioic acid)(PXDDA). The physicochemical and biological properties of the new PXDDA polymer were characterized. Fourier transform infrared (FTIR) confirmed the formation of ester bonding in the polymer structure, and thermal analysis demonstrated that the polymer was completely amorphous. The polymer shows high elasticity. Increasing the molar ratio of dodecanedioic acid resulted in higher hydrophobicity and lower glass transition temperature. Further, the polymer degradation and in vitro dye release studies revealed that the degradation and dye release from the polymer became slower when the amount of dodecanedioic acid in the composite increased.
Show less - Date Issued
- 2019
- PURL
- http://purl.flvc.org/fau/fd/FA00013306
- Subject Headings
- Drug Delivery Systems, Polymers, Tissue Engineering, Xylitol
- Format
- Document (PDF)
- Title
- Development of a Thermosensitive Trimethyl Chitosan Hydrogel for in situ Tissue Engineering.
- Creator
- Qian, Enze, Kang, Yunqing, Florida Atlantic University, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
- Abstract/Description
-
Chitosan was widely studied for applications in tissue regeneration, because of its biodegradability and biocompatibility. However, its insolubility in a neutral solution and long gelation time limit its wide application in tissue engineering. In this thesis, a new chitosan-based biomaterial was synthesized, and its chemical structure and solubility were characterized. Afterwards, the gelation properties (crosslinker, crosslink time, swelling ratio, drug release and biocompatibility) of TMC...
Show moreChitosan was widely studied for applications in tissue regeneration, because of its biodegradability and biocompatibility. However, its insolubility in a neutral solution and long gelation time limit its wide application in tissue engineering. In this thesis, a new chitosan-based biomaterial was synthesized, and its chemical structure and solubility were characterized. Afterwards, the gelation properties (crosslinker, crosslink time, swelling ratio, drug release and biocompatibility) of TMC material was investigated. Results show that TMC has higher water solubility than chitosan. The TMC liquid solution can transform to a hydrogel quickly at body temperature. The formed hydrogel controlled the release of the model protein. Cytotoxicity result shows the cationic TMC hydrogel brings a toxic effect on stromal cells but it may have the potential to inhibit bacteria or cancer cells, although more studied are required to confirm its potential functions. In summary, this new TMC hydrogel has a promising potential in biomedical fields.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FA00013095
- Subject Headings
- Tissue engineering., Chitosan--Biotechnology., Hydrogels.
- Format
- Document (PDF)
- Title
- DEVELOPMENT OF MULTIFUNCTIONAL BETA-TRICALCIUM PHOSPHATE SCAFFOLDS FOR ANGIOGENESIS AND OSTEOGENESIS.
- Creator
- Qian, Enze, Kang, Yunqing, Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Rapid and efficient vascularization is still a considerable challenge of a tissue engineered β-tricalcium phosphate (β-TCP) scaffold. To overcome this challenge, branched channels were created in the porous scaffold to stimulate the instant flow of blood supply. The branched channeled porous β-TCP scaffold was fabricated using 3D printing and template-casting method. Human bone mesenchymal stem cells (hBMSC) and human umbilical vein endothelial cells (HUVEC) were seeded in the scaffolds and...
Show moreRapid and efficient vascularization is still a considerable challenge of a tissue engineered β-tricalcium phosphate (β-TCP) scaffold. To overcome this challenge, branched channels were created in the porous scaffold to stimulate the instant flow of blood supply. The branched channeled porous β-TCP scaffold was fabricated using 3D printing and template-casting method. Human bone mesenchymal stem cells (hBMSC) and human umbilical vein endothelial cells (HUVEC) were seeded in the scaffolds and characterized through double-stranded DNA (dsDNA) assay, alkaline phosphatase (ALP) assay and cell migration. Scaffolds were then implanted in the subcutaneous pockets in mice. Hematoxylin and eosin staining and Immunohistochemical staining on vascularization, bone-related markers were carried out. Results showed that branched channels significantly promoted HUVECs’ infiltration, migration, proliferation, and angiogenesis and also promote the proliferation and osteogenesis differentiation of hBMSCs. Scaffolds did not show significant pro-inflammatory effects. In vivo results showed that in the early stage after implantation, cells significantly migrated into branched channeled scaffolds compared to non-channeled and straight channeled scaffolds. More and matured blood vessels formed in the branched channeled scaffolds compared to in non-channeled and straight channeled scaffolds. Besides promoting vascularization, the branched channels also stimulated the infiltration of bone-related cells into the scaffolds. These results suggested that the geometric design of branched channels in the porous β-TCP scaffold promoted rapid vascularization and potentially stimulated bone cell recruitment. To further enhance the function of the scaffold to promote the MSCs differentiation, MnO2 hollow and solid nanoparticles were doped into the scaffold with different concentrations.
Show less - Date Issued
- 2023
- PURL
- http://purl.flvc.org/fau/fd/FA00014193
- Subject Headings
- Tissue engineering, Bone Regeneration, Beta-tricalcium phosphate
- Format
- Document (PDF)
- Title
- ENGINEERING CHANNELS IN POROUS CALCIUM PHOSPHATE BIOCERAMIC SCAFFOLDS FOR BONE TISSUE REGENERATION.
- Creator
- Wang, Xuesong, Kang, Yunqing, Florida Atlantic University, Department of Ocean and Mechanical Engineering, College of Engineering and Computer Science
- Abstract/Description
-
Inadequate nutrition exchange and slow transportation in a porous scaffold often resulted in insufficient vasculature formation, which hindered rapid bone regeneration. In this study, interconnected porous beta-tricalcium phosphate (b-TCP) scaffolds with channeled geometry were fabricated. In vitro fluid transportation and degradation of the scaffolds were performed. Cell attachment, migration, proliferation, and differentiation were carried out under both static and dynamic culturing...
Show moreInadequate nutrition exchange and slow transportation in a porous scaffold often resulted in insufficient vasculature formation, which hindered rapid bone regeneration. In this study, interconnected porous beta-tricalcium phosphate (b-TCP) scaffolds with channeled geometry were fabricated. In vitro fluid transportation and degradation of the scaffolds were performed. Cell attachment, migration, proliferation, and differentiation were carried out under both static and dynamic culturing conditions. A computational simulation model and a series of immunofluorescent staining were implemented to understand the mechanism of cell behavior in respond to different scaffolds geometry. We then implanted scaffolds into rat critical-sized calvarial defects to further evaluate channels’ function on bone regeneration in vivo. Results showed that multiple channeled geometry significantly accelerated the release of Ca2+ and increased the fluid diffusion efficiency. Moreover, multiple channels promoted human umbilical vein endothelial cells (HUVECs) infiltration, migration, besides prominently promoted alkaline phosphatase (ALP) activity, and up-regulated osteogenic gene expression in human bone marrow mesenchymal stem cells (hBMSCs) at both static and dynamic culturing conditions in vitro. The expression of both cell migration related protein a5 and angiogenesis related protein CD31 were upregulated by multiple channels in HUVECs. And the expression of mechanosensing markers, focal adhesion kinase (FAK), polymeric filamentous actin (Factin), and Yes-associated protein-1 (YAP-1) were highly stimulated by multiple channels in hBMSCs. The in vivo implantation and characterization results demonstrated more bone formation inside multiple-channeled scaffolds compared to non-channeled scaffolds. Multiple channels accelerated collagen type I, Bone Sialoprotein (Bsp), Osteocalcin (OC) protein expression prominently. The angiogenesis related protein CD31 staining displayed longer and more vasculature structures on multiple-channeled scaffolds compared to nonchanneled scaffolds. Fluorescent images of the fluorochrome labeled samples exhibited considerably more mineral deposition on multiple-channeled scaffolds than non-channeled scaffolds. All the findings suggested that the addition of multiple channels in the porous b-TCP scaffold is very promising approach to promote vascularization and bone tissue regeneration.
Show less - Date Issued
- 2019
- PURL
- http://purl.flvc.org/fau/fd/FA00013426
- Subject Headings
- Tissue Engineering, Bone and Bones, Bone regeneration, Calcium Phosphates, Biocompatible Materials
- Format
- Document (PDF)
- Title
- Engineering of tissue inhibitor of metalloproteinases mutants as potential therapeutics.
- Creator
- Nagase, Hideaki, Brew, Keith
- Abstract/Description
-
Matrix metalloproteinases (MMPs) play a central role in many biological processes such as development, morphogenesis and wound healing, but their unbalanced activities are implicated innumerous disease processes such as arthritis, cancer metastasis, atherosclerosis, nephritis and fibrosis. One of the key mechanisms to control MMP activities is inhibition by endogenous inhibitors called tissue inhibitors of metalloproteinases (TIMPs). This review highlights the structures and inhibition...
Show moreMatrix metalloproteinases (MMPs) play a central role in many biological processes such as development, morphogenesis and wound healing, but their unbalanced activities are implicated innumerous disease processes such as arthritis, cancer metastasis, atherosclerosis, nephritis and fibrosis. One of the key mechanisms to control MMP activities is inhibition by endogenous inhibitors called tissue inhibitors of metalloproteinases (TIMPs). This review highlights the structures and inhibition mechanism of TIMPs, the biological activities of TIMPs, the unique properties of TIMP-3, and the altered specificity towards MMPs achieved by mutagenesis. A potential therapeutic use of TIMP variants is discussed.
Show less - Date Issued
- 2002-04-02
- PURL
- http://purl.flvc.org/fcla/dt/3327266
- Subject Headings
- Gene Therapy --Methods, Genetic Engineering --methods, Protein Structure, Tertiary, Rheumatic Diseases --therapy, Tissue Inhibitor of Metalloproteinases, Wound Healing --physiology, Metalloproteinases --Inhibitors --Therapeutic use
- Format
- Document (PDF)