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(1 - 9 of 9)
- Title
- Characterization, biosynthetic studies and subcellular localization of sterols from the marine sponge Teichaxinella morchella.
- Creator
- Williams, Amanda Joy, Florida Atlantic University, Kerr, Russell G.
- Abstract/Description
-
A-nor sterols have been isolated and characterized from the marine sponge Teichaxinella morchella using High Performance Liquid Chromatography, Nuclear Magnetic Resonance, and Mass Spectrometry. A cell free extract of T. morchella was used to determine the biosynthetic origin of the sterols. Incubation experiments with radiolabled Farnesyl Pyrophosphate and cholesterol showed that the sterols are coming from a dietary source. Subcellular localization studies showed that the sterols are part...
Show moreA-nor sterols have been isolated and characterized from the marine sponge Teichaxinella morchella using High Performance Liquid Chromatography, Nuclear Magnetic Resonance, and Mass Spectrometry. A cell free extract of T. morchella was used to determine the biosynthetic origin of the sterols. Incubation experiments with radiolabled Farnesyl Pyrophosphate and cholesterol showed that the sterols are coming from a dietary source. Subcellular localization studies showed that the sterols are part of the cell membrane.
Show less - Date Issued
- 2001
- PURL
- http://purl.flvc.org/fcla/dt/12865
- Subject Headings
- Sponges, Sterols
- Format
- Document (PDF)
- Title
- 9,11-secogorgosterol biosynthesis in gorgonians.
- Creator
- Kellman, Jaelle, Florida Atlantic University, Kerr, Russell G., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
9,11-secogorgosterol is a secondary metabolite from the gorgonian Pseudopterogorgia americana which acts as a chemical defense. The soft coral lives in a symbiotic association with unicellular algae known as zooxanthellae. A biosynthetic investigation, using in vivo and in vitro methods, has resulted in the identification of the metabolic precursor of 9,11-secogorgosterol as gorgosterol. This finding is significant as it indicates that the conversion of gorgosterol to 9,11-secogorgosterol is...
Show more9,11-secogorgosterol is a secondary metabolite from the gorgonian Pseudopterogorgia americana which acts as a chemical defense. The soft coral lives in a symbiotic association with unicellular algae known as zooxanthellae. A biosynthetic investigation, using in vivo and in vitro methods, has resulted in the identification of the metabolic precursor of 9,11-secogorgosterol as gorgosterol. This finding is significant as it indicates that the conversion of gorgosterol to 9,11-secogorgosterol is due to gorgonian metabolism. Since gorgosterol is known to be a product of zooxanthellae metabolism, this would be the first example of a defensive secondary metabolite being produced by two organisms living in symbiosis. A viable acetone powder has been generated from the crude cell-free extract and has demonstrated the efficient transformation of gorgosterol to 9, 11-secogorgosterol. This indicates possible future value as a synthetic tool for secosterol production.
Show less - Date Issued
- 1995
- PURL
- http://purl.flvc.org/fcla/dt/15180
- Subject Headings
- Sterols--Synthesis, Alcyonacea
- Format
- Document (PDF)
- Title
- Chemotaxonomy of the marine sponge Xestospongia muta.
- Creator
- Reddy, Manoj Kumar., Florida Atlantic University, Kerr, Russell G.
- Abstract/Description
-
Chemical analysis of large number of specimens of the sponge Xestospongia muta have revealed the existence of three distinct sterol chemotypes. Since sponges are well known to contain a diverse array of all classes of lipids, the utility of fatty acid composition as a chemotaxonomic tool for Xestospongia muta has been addressed. Further the biosynthetic capabilities of the three chemotypes are being evaluated for possible taxonomic use. Further the utility of TLC and NMR as additional...
Show moreChemical analysis of large number of specimens of the sponge Xestospongia muta have revealed the existence of three distinct sterol chemotypes. Since sponges are well known to contain a diverse array of all classes of lipids, the utility of fatty acid composition as a chemotaxonomic tool for Xestospongia muta has been addressed. Further the biosynthetic capabilities of the three chemotypes are being evaluated for possible taxonomic use. Further the utility of TLC and NMR as additional taxonomic tools has also been evaluated.
Show less - Date Issued
- 1995
- PURL
- http://purl.flvc.org/fcla/dt/15167
- Subject Headings
- Chemotaxonomy, Sponges, Sterols
- Format
- Document (PDF)
- Title
- New sterol ester from a deep water marine sponge, Xestospongia sp.
- Creator
- Gunasekera, Sarath P., Cranick, S., Pomponi, Shirley A.
- Date Issued
- 1991
- PURL
- http://purl.flvc.org/FCLA/DT/3332980
- Subject Headings
- Sponges, Sterols, Marine natural products
- Format
- Document (PDF)
- Title
- Biosynthesis and enzymology of secosteroids from Pseudopterogorgia americana.
- Creator
- Head, Kevin, Florida Atlantic University, Kerr, Russell G.
- Abstract/Description
-
9,11-Secosteroids are biologically active secondary metabolites from the marine invertebrate, Pseudopterogorgia americana. They are produced in vitro through an incubation of the steroid substrate with a cell-free extract of P. americana containing the necessary enzymes. Further optimization of this incubation was attempted through adjustment of incubation reagents and cofactors. The enzymes responsible for secosteroid production were partially purified through chromatography in an effort to...
Show more9,11-Secosteroids are biologically active secondary metabolites from the marine invertebrate, Pseudopterogorgia americana. They are produced in vitro through an incubation of the steroid substrate with a cell-free extract of P. americana containing the necessary enzymes. Further optimization of this incubation was attempted through adjustment of incubation reagents and cofactors. The enzymes responsible for secosteroid production were partially purified through chromatography in an effort to isolate and purify these enzymes. Finally, experiments with radiolabeled gorgosterol led to the elucidation and isolation of a key secosteroid precursor, 9,11-dihydroxygorgosterol.
Show less - Date Issued
- 1999
- PURL
- http://purl.flvc.org/fcla/dt/15711
- Subject Headings
- Alcyonacea, Enzymes--Synthesis, Sterols
- Format
- Document (PDF)
- Title
- Biochemical and morphologicalheterogeneity in the Caribbean sponge Xestospongia muta (petrosida: Petrosiidae).
- Creator
- Kerr, Russell G., Kelly-Borges, M., Harbor Branch Oceanographic Institute
- Date Issued
- 1994
- PURL
- http://purl.flvc.org/fau/fd/FA00007380
- Subject Headings
- Sponges--Caribbean Sea, Morphology, Biochemistry, Sterols
- Format
- Document (PDF)
- Title
- New antiviral sterol disulfate ortho esters from the marine sponge Petrosia weinbergi.
- Creator
- Koehn, F. E., Gunasekera, M., Cross, S. S., Harbor Branch Oceanographic Institute
- Date Issued
- 1991
- PURL
- http://purl.flvc.org/FCLA/DT/3332977
- Subject Headings
- Sponges, Sterols, Marine natural products, Antiviral agents
- Format
- Document (PDF)
- Title
- Sterols of the marine sponge Petrosia weinbergi: implications for the absolute configurations of the antiviral orthoesterols and weinbersterols.
- Creator
- Giner, José-Luis, Gunasekera, Sarath P., Pomponi, Shirley A.
- Date Issued
- 1999
- PURL
- http://purl.flvc.org/FCLA/DT/3158771
- Subject Headings
- Sponges, Sterols --Analysis, Steroids, Biosynthesis, Marine metabolites
- Format
- Document (PDF)
- Title
- A new cytotoxic sterol methoxymethyl ether from a deep water marine sponge Scleritoderma sp. cf. paccardi.
- Creator
- Gunasekera, Sarath P., Kelly-Borges, M., Longley, Ross E., Harbor Branch Oceanographic Institute
- Date Issued
- 1996
- PURL
- http://purl.flvc.org/FCLA/DT/3319090
- Subject Headings
- Sponges --Research, Sterols, Marine natural products, NMR spectroscopy
- Format
- Document (PDF)
