Current Search:  Staphylococcus aureus (x)

View All Items

  • CSV Spreadsheet
(1 - 2 of 2)
Distribution and Survival of Micrococcaceae in Marine Waters of Southeast Florida (Boca Raton and Pompano Beach)
Title
Distribution and Survival of Micrococcaceae in Marine Waters of Southeast Florida (Boca Raton and Pompano Beach).
Creator
Thompson, Mary C., Hoffmann, H. A., Florida Atlantic University
Abstract/Description
This study was undertaken to determine the distribution and possible source of Staphylococcus aureus, s. epidermidis, other Micrococcaceae , and enterococci recovered from samples of water collected at the following locations: the Pompano Beach sewage outfall, the Intracoastal Waterway, 5 miles due east of the Boca Raton Inlet, over the 65 ft Boca Raton reef, and in 5 ft of water along the Boca Raton beach. It was also of interest to study the feasibility of employing s. aureus as an...
Show moreThis study was undertaken to determine the distribution and possible source of Staphylococcus aureus, s. epidermidis, other Micrococcaceae , and enterococci recovered from samples of water collected at the following locations: the Pompano Beach sewage outfall, the Intracoastal Waterway, 5 miles due east of the Boca Raton Inlet, over the 65 ft Boca Raton reef, and in 5 ft of water along the Boca Raton beach. It was also of interest to study the feasibility of employing s. aureus as an indicator of fecal pollution in the marine environment. Neither staphylococci nor enterococci were recovered from 5 samples of beach sand collected from the high tide line to the low tide line. Only low concentrations of staphylococci were recovered from samples collected from the Intracoastal Waterway. The staphylococci recovered from the marine locations do not appear to be true marine bacteria. They do not appear to be originating from bathers, beach washings or the Intracoastal Waterway. Sampling was inadequate to reach definite conclusions on the origin of the Micrococcaceae recovered in the ocean. However, these data indicate the staphylococci originate from the Pompano Beach sewage outfall. Staphylococcus aureus appears to be more resistant to the conditions of the marine environment than the other Micrococcaceae, enterococci or other sewage bacteria recovered on Mannitol Salt Agar and m-Enterococcus Agar. The mean per cent recovery of S. aureus increases with increased distances downstream from the outfall to the 65 ft reef and sampling site 5 miles offshore. ~· aureus appears to be a good indicator of fecal pollution in the marine environment. Survival studies of washed cells of a coagulase positive Staphylococcus aureus run in autoclaved sea water and autoclaved Intracoastal water gave mean decimal reduction times of 16.6 hr and 11.4 hr, respectively. Staphylococci were not recovered from Bryothamnion triguetrum,Caulerpa sertularioides f. brevipes, Dasycladus vermicularis or Padina sanctae-crucis. Ten Micrococcaceae recovered from the ocean were not inhibited by extracts prepared from these algae. Intact segments of these algae possessed antibacterial activity against most, if not all of these Micrococcaceae.
Show less
Date Issued
1969
PURL
http://purl.flvc.org/fau/fd/FA00000840
Subject Headings
Staphylococcus aureus, Sewage disposal in the ocean--Florida, Marine pollution
Format
Document (PDF)
EVALUATION OF MICROBIOLOGICAL METHODS FOR USE IN MARINE WATERS: ENUMERATION OF STAPHYLOCOCCI BY A FLUORESCENT ANTIBODY TECHNIQUE, AND ISOTOPIC DETERMINATION OF BACTERIAL METABOLIC POTENTIAL
Title
AN EVALUATION OF MICROBIOLOGICAL METHODS FOR USE IN MARINE WATERS: ENUMERATION OF STAPHYLOCOCCI BY A FLUORESCENT ANTIBODY TECHNIQUE, AND ISOTOPIC DETERMINATION OF BACTERIAL METABOLIC POTENTIAL.
Creator
BUCHANAN, IAN ELLIS., Florida Atlantic University, Hoffmann, H. A.
Abstract/Description
Two methods, a membrane filter-fluorescent antibody technique for detection of Staphylococcus aureus, and isotopic determination of relative heterotrophic activity, were compared with a standard technique based on the recovery of coliform organisms for evaluation as indices of marine pollution. Water samples were collected from coastal areas of Pompano Beach and Boca Raton, Florida. Coliform and staphylococci survival in seawater was studied under laboratory conditions. The effect of exposure...
Show moreTwo methods, a membrane filter-fluorescent antibody technique for detection of Staphylococcus aureus, and isotopic determination of relative heterotrophic activity, were compared with a standard technique based on the recovery of coliform organisms for evaluation as indices of marine pollution. Water samples were collected from coastal areas of Pompano Beach and Boca Raton, Florida. Coliform and staphylococci survival in seawater was studied under laboratory conditions. The effect of exposure to seawater on substrate uptake by coliforms and other sewage microorganisms was studied using isotopic methods. The results of these studies support the feasibility of using S· aureus as a long-range indicator of marine pollution. Under natural conditions, S· aureus appears more resistant to seawater than coliform organisms, although the reverse was found under laboratory conditions. Seawater inhibited uptake of glucose and lactose by Escherichia coli and other sewage organisms. Relative heterotrophic potentials in terms of glucose and lactose uptake velocities were not significantly affected in coastal waters by the influx of bacteria and nutrients in sewage outfall effluent.
Show less
Date Issued
1973
PURL
http://purl.flvc.org/fcla/dt/13557
Subject Headings
Staphylococcus aureus, Marine pollution, Sewage disposal in the ocean, Sewage--Microbiology
Format
Document (PDF)