Current Search:  Sewage--Microbiology (x)

View All Items

  • CSV Spreadsheet
(1 - 2 of 2)
A Study of the Longevity of f2 Bacteriophage in Marine and Fresh Water Sewage Effluent, and Its Use as a Biological Indicator of Pollution
Title
A Study of the Longevity of f2 Bacteriophage in Marine and Fresh Water Sewage Effluent, and Its Use as a Biological Indicator of Pollution.
Creator
Twist, Ernest Michael, Waddell, Glenn H., Florida Atlantic University
Abstract/Description
The significance of the transmission of animal viruses in sewage polluted fresh and marine waters becomes an increasingly important factor as more and more evidence is presented to support the fact that in the case of many viral types one virus particle constitutes an infective dose. Therefore it is very important that a method of determining the extent of viral pollution in sewage polluted bodies of marine and fresh water be found. The longevity of f2 bacteriophage in both marine and fresh...
Show moreThe significance of the transmission of animal viruses in sewage polluted fresh and marine waters becomes an increasingly important factor as more and more evidence is presented to support the fact that in the case of many viral types one virus particle constitutes an infective dose. Therefore it is very important that a method of determining the extent of viral pollution in sewage polluted bodies of marine and fresh water be found. The longevity of f2 bacteriophage in both marine and fresh water sewage polluted samples was determined, and shown to be 3 to 4 days for marine water and 8 days for fresh water. It was also shown that the culture medium in which the f2 phage was produced, and the larger macromolecular polymers commonly found associated with sewage had a favorable affect in increasing the longevity of f2 phage. It was shown that f2 bacteriophage could be used to follow the initial dispersion of the sewage boil arising from an ocean outfall sewerage system. From these results it was concluded that f2 bacteriophage could be used for more detailed studies concerned with the transmission of viruses in sewage polluted waters.
Show less
Date Issued
1968
PURL
http://purl.flvc.org/fau/fd/FA00000841
Subject Headings
Sewage disposal in the ocean, Marine pollution, Bacteriophages, Sewage--Microbiology
Format
Document (PDF)
EVALUATION OF MICROBIOLOGICAL METHODS FOR USE IN MARINE WATERS: ENUMERATION OF STAPHYLOCOCCI BY A FLUORESCENT ANTIBODY TECHNIQUE, AND ISOTOPIC DETERMINATION OF BACTERIAL METABOLIC POTENTIAL
Title
AN EVALUATION OF MICROBIOLOGICAL METHODS FOR USE IN MARINE WATERS: ENUMERATION OF STAPHYLOCOCCI BY A FLUORESCENT ANTIBODY TECHNIQUE, AND ISOTOPIC DETERMINATION OF BACTERIAL METABOLIC POTENTIAL.
Creator
BUCHANAN, IAN ELLIS., Florida Atlantic University, Hoffmann, H. A.
Abstract/Description
Two methods, a membrane filter-fluorescent antibody technique for detection of Staphylococcus aureus, and isotopic determination of relative heterotrophic activity, were compared with a standard technique based on the recovery of coliform organisms for evaluation as indices of marine pollution. Water samples were collected from coastal areas of Pompano Beach and Boca Raton, Florida. Coliform and staphylococci survival in seawater was studied under laboratory conditions. The effect of exposure...
Show moreTwo methods, a membrane filter-fluorescent antibody technique for detection of Staphylococcus aureus, and isotopic determination of relative heterotrophic activity, were compared with a standard technique based on the recovery of coliform organisms for evaluation as indices of marine pollution. Water samples were collected from coastal areas of Pompano Beach and Boca Raton, Florida. Coliform and staphylococci survival in seawater was studied under laboratory conditions. The effect of exposure to seawater on substrate uptake by coliforms and other sewage microorganisms was studied using isotopic methods. The results of these studies support the feasibility of using S· aureus as a long-range indicator of marine pollution. Under natural conditions, S· aureus appears more resistant to seawater than coliform organisms, although the reverse was found under laboratory conditions. Seawater inhibited uptake of glucose and lactose by Escherichia coli and other sewage organisms. Relative heterotrophic potentials in terms of glucose and lactose uptake velocities were not significantly affected in coastal waters by the influx of bacteria and nutrients in sewage outfall effluent.
Show less
Date Issued
1973
PURL
http://purl.flvc.org/fcla/dt/13557
Subject Headings
Staphylococcus aureus, Marine pollution, Sewage disposal in the ocean, Sewage--Microbiology
Format
Document (PDF)