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Immunoaffinity purification of albumin from fish serum
Title
Immunoaffinity purification of albumin from fish serum.
Creator
Apter, Christine V., Florida Atlantic University, Hartmann, James X.
Abstract/Description
This study describes an affinity method which purifies albumin from fish serum. Hybridoma clones producing antibodies specific to fish albumin have been screened and propagated and their antibodies coupled to a cross-linked agarose gel matrix which has been activated with N-hydroxysuccinimide. The resulting gel with its ligand attached was then used to purify albumin from fish serum. The procedure was optimized for coupling, sample application and elution. The coupling optimization using...
Show moreThis study describes an affinity method which purifies albumin from fish serum. Hybridoma clones producing antibodies specific to fish albumin have been screened and propagated and their antibodies coupled to a cross-linked agarose gel matrix which has been activated with N-hydroxysuccinimide. The resulting gel with its ligand attached was then used to purify albumin from fish serum. The procedure was optimized for coupling, sample application and elution. The coupling optimization using varying pH values resulted in over 90% coupling efficiencies. The sample application optimization showed yields of 2 mg of protein per ml of serum at 1:5 dilution of sample. Elution conditions were evaluated using varying pH and chaotropic agent concentrations. The optimal elution conditions (pH 4.0) resulted in a yield of 0.2 mg/ml of gel with several impurities. Elution at pH 3.0 resulted in a very pure product with low yield (.02 mg/ml of gel) and a loss of activity.
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Date Issued
1998
PURL
http://purl.flvc.org/fcla/dt/15568
Subject Headings
Serum albumin--Purification, Immune serums
Format
Document (PDF)
Use of sera from humans and dolphins with lacaziosis and sera from experimentally infected mice forwestern blot analyses of Lacazia loboi antigens
Title
Use of sera from humans and dolphins with lacaziosis and sera from experimentally infected mice forwestern blot analyses of Lacazia loboi antigens.
Creator
Mendoza, L.,, Belone, A. F. F., Vilela, R., Rehtanz, Manuela, Bossart, Gregory D., Reif, John S., Fair, Patricia A., Durden, Wendy N., St. Leger, J., Travassos, L. R., Rosa, P. S., Harbor Branch Oceanographic Institute
Date Issued
2008
PURL
http://purl.flvc.org/fau/fd/FA00007038
Subject Headings
Lobomycosis, Blotting, Western, Antigens, Serum
Format
Document (PDF)
Serum chemistry values for nestling bald eagles (Haliaeetus leucocephalus) in Florida Bay, Everglades National Park
Title
Serum chemistry values for nestling bald eagles (Haliaeetus leucocephalus) in Florida Bay, Everglades National Park.
Creator
Mealey, Brian K., Parks, G. M., Pages, C. M., Millsap, B. A., Bass, O. L., Bossart, Gregory D., Harbor Branch Oceanographic Institute
Date Issued
2004
PURL
http://purl.flvc.org/FCLA/DT/3340512
Subject Headings
Haliaeetus leucocephalus, Bald eagle, Serum--Analysis, Hematology, Everglades National Park (Fla.), Florida Bay (Fla.)
Format
Document (PDF)
Serum chemistry values and isolated microbial organisms for Swallow-tailed Kite, Elanoides forficatus, nestlings in southeastern United States
Title
Serum chemistry values and isolated microbial organisms for Swallow-tailed Kite, Elanoides forficatus, nestlings in southeastern United States.
Creator
Mealey, Brian K., Bossart, Gregory D., Meyer, K. D., Harbor Branch Oceanographic Institute
Date Issued
2006
PURL
http://purl.flvc.org/FCLA/DT/3183172
Subject Headings
American swallow-tailed kite, Serum--Analysis, Birds of prey --Health, Hematology, Microorganisms
Format
Document (PDF)