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Assessment of multiple paternity for the queen conch, Strombus gigas
Title
Assessment of multiple paternity for the queen conch, Strombus gigas.
Creator
Harris, Chris., Harriet L. Wilkes Honors College
Abstract/Description
The commercially important queen conch, Strombus gigas, has been observed copulating with multiple partners and laying multiple egg masses during a reproductive season (Randall, 1964). While multiple paternity has been confirmed using microsatellite based genetic analysis for a variety of other gastropods, this technique has not been employed for S. gigas. Determining whether or not this species is capable of multiple paternity is important to understanding and maintaining genetic diversity...
Show moreThe commercially important queen conch, Strombus gigas, has been observed copulating with multiple partners and laying multiple egg masses during a reproductive season (Randall, 1964). While multiple paternity has been confirmed using microsatellite based genetic analysis for a variety of other gastropods, this technique has not been employed for S. gigas. Determining whether or not this species is capable of multiple paternity is important to understanding and maintaining genetic diversity of natural and captive populations. While an assessment of multiple paternity is the ultimate goal of this study, for my thesis, I have completed preliminary work which includes perfecting methods of tissue collection, DNA extraction, and DNA amplification with six non-labeled polymorphic microsatellite molecular markers, using cultured Strombus gigas animals. In addition, I collected tissue and extracted DNA from three wild S. gigas adult females and their egg masses from Pelican Shoal in the Florida Keys.
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Date Issued
2008
PURL
http://purl.flvc.org/FAU/77671
Subject Headings
Gastropoda, Habitat, Queen conch, Conservation, Biological diversity, Marine biology, Mariculture
Format
Document (PDF)
Toxicity and availability of copper and zinc to queen conch
Title
Toxicity and availability of copper and zinc to queen conch: implications for larval recruitment in the Florida Keys.
Creator
Garr, Amber L., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
The presence of heavy metals and other pollutants is detrimental to marine ecosystems. The queen conch, once an important fisheries species in the Florida Keys, has not sufficiently recovered after a 25-year fishery closure. Research has shown high levels of copper and zinc in the gonads and digestive glands of adult conch found in the nearshore waters. Four sites relevant to queen conch larval recruitment were tested in 2010 for the presence of copper and zinc in the water, phytoplankton,...
Show moreThe presence of heavy metals and other pollutants is detrimental to marine ecosystems. The queen conch, once an important fisheries species in the Florida Keys, has not sufficiently recovered after a 25-year fishery closure. Research has shown high levels of copper and zinc in the gonads and digestive glands of adult conch found in the nearshore waters. Four sites relevant to queen conch larval recruitment were tested in 2010 for the presence of copper and zinc in the water, phytoplankton, sediment, and seagrass epiphytes over seven months. Both metals were detected in all sample types and no seasonal or geographical differences were detected. Surface water concentrations from the field were used to conduct acute and chronic toxicity tests on various ages of queen conch larvae and their phytoplankton food source. When zinc concentrations (0-40 (So(Bg/L) similar to those measured in situ were used, there was no significant impact on conch larval survival although some velar lobe de velopment was impaired. However, field concentrations of copper (0-15 (So(Bg/L), which often surpassed water quality standards, negatively impacted growth, survival, and development of the larvae. Chronic exposure to copper, through the water and food, disrupted the metamorphic success of competent larvae and decreased post-metamorphosis survival. Exposure to copper at later life stages increased mortality, suggesting that heavy metals have a negative effect on larval recruitment in localized areas of the Florida Keys. Structural equation modeling revealed that copper and zinc are moving through the systems differently and are best represented by two different models.
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Date Issued
2012
PURL
http://purl.flvc.org/FAU/3342102
Subject Headings
Gastropoda, Habitat, Queen conch, Conservation, Marine invertebrates, Larvae, Trace elements, Environmental aspects
Format
Document (PDF)