Current Search: Plasmids -- Genetics (x)
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Title
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Progress towards genetic engineering of an EMF-responsive plasmid in yeast.
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Creator
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Crenshaw, Karin Ann., Florida Atlantic University, Binninger, David
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Abstract/Description
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Despite the many epidemiological studies which have shown some effects of EMF on biological systems, there has yet to be any data that indicates the molecular mechanisms by which this effect takes place. My goal was to genetically engineer a yeast strain that would have a clear biological effect to the EMF's. The strategy involved using a yeast strain which requires histone function from a plasmid, and the plasmid construct that has a Gal1 promoter controlling the histone function. The...
Show moreDespite the many epidemiological studies which have shown some effects of EMF on biological systems, there has yet to be any data that indicates the molecular mechanisms by which this effect takes place. My goal was to genetically engineer a yeast strain that would have a clear biological effect to the EMF's. The strategy involved using a yeast strain which requires histone function from a plasmid, and the plasmid construct that has a Gal1 promoter controlling the histone function. The plasmid construct could then be engineered to contain a promoter sequence for a known EMF-repressed gene in yeast, which would control the histone production. Without a functional histone gene, the cells will die, and the effects will be easily visualized. Although the genetic screening for the desired transformants appeared to work, the molecular analysis of those transformants did not show the promoter insertion. There are a few possible reasons for why this happened, including possible reversions from one of the original mutations of the chromosomal histone H4 genes, or the mutation of the Gal1 promoter which would no longer repress the histone H4 gene and allow the cells to grow on glucose.
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Date Issued
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1998
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PURL
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http://purl.flvc.org/fcla/dt/15572
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Subject Headings
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Electromagnetism--Physiological effect, Genetic engineering, Plasmids--Genetics
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Format
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Document (PDF)
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Title
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Optimizing atrazine catabolism in Pseudomonas sp. strain ADP.
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Creator
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Anderson, Robert., Harriet L. Wilkes Honors College
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Abstract/Description
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Atrazine is a popular herbicide used in over 80 countries to inhibit the growth of broadleaf and grassy weeds. Atrazine is a common pollutant in soil and groundwater, and high concentrations of atrazine cause developmental defects in fish, amphibians, and birds. The bacteria Pseudomonas sp. strain ADP (P.ADP) uses atrazine as a nitrogen source by transforming atrazine to ammonia through a number of enzymatic reactions. In this project I measured the growth and atrazine degradation of P.ADP in...
Show moreAtrazine is a popular herbicide used in over 80 countries to inhibit the growth of broadleaf and grassy weeds. Atrazine is a common pollutant in soil and groundwater, and high concentrations of atrazine cause developmental defects in fish, amphibians, and birds. The bacteria Pseudomonas sp. strain ADP (P.ADP) uses atrazine as a nitrogen source by transforming atrazine to ammonia through a number of enzymatic reactions. In this project I measured the growth and atrazine degradation of P.ADP in media with atrazine as the sole nitrogen source. A mutant strain isolated after mutagenesis with UV light showed faster growth and reached higher densities than the control strain. A series of mating experiments were performed to determine whether the growth mutation was on the atrazine degrading plasmid or in the chromosome. The limitations and potential of P.ADP for atrazine degradation are discussed.
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Date Issued
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2010
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PURL
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http://purl.flvc.org/FAU/3334252
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Subject Headings
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Plasmids, Genetics, Alginates, Physiological effect, Microbial ecology, Biotransformation (Metabolism)
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Format
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Document (PDF)
