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Characterization of Methionine Sulfoxide Reductases A and Bs from Tobacco Plant
Title
Characterization of Methionine Sulfoxide Reductases A and Bs from Tobacco Plant.
Creator
Ding, Di, Zhang, Xing-Hai, Florida Atlantic University
Abstract/Description
One methionine sulfoxide reductase A (TMSRA) and two methionine sulfoxide reductase Bs (TMSRB 1 and TMSRB2) were isolated from tobacco plants. TMSRA showed specificity for the reduction of Met-(S)-SO and both TMSRBs were specific for the reduction of Met-(R)-SO. TMSRA was the cytosolic form and both TMSRBs were plastid forms based on sequence comparison and expression tests. TMSRA and TMSRB2 could use either thioredoxin (TRX) or dithiothreitol (DTT) as reducing system, while TMSRB 1 showed...
Show moreOne methionine sulfoxide reductase A (TMSRA) and two methionine sulfoxide reductase Bs (TMSRB 1 and TMSRB2) were isolated from tobacco plants. TMSRA showed specificity for the reduction of Met-(S)-SO and both TMSRBs were specific for the reduction of Met-(R)-SO. TMSRA was the cytosolic form and both TMSRBs were plastid forms based on sequence comparison and expression tests. TMSRA and TMSRB2 could use either thioredoxin (TRX) or dithiothreitol (DTT) as reducing system, while TMSRB 1 showed little activity with TRX but much more activity with DTT, which was similar to the mitochondrial MSRB2 from mammals. Ferredoxin (FD) is not the reducing system for Msrs, but might reflect the redox status in the cell and control the activity of Msrs indirectly.
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Date Issued
2006
PURL
http://purl.flvc.org/fau/fd/FA00000746
Subject Headings
Proteins--Chemical modification, Genetic regulation, Plant genetic engineering, Antioxidants
Format
Document (PDF)
Enhancement of nutrional value in tomato plants
Title
Enhancement of nutrional value in tomato plants.
Creator
Hill, William, Zhang, Xing-Hai
Date Issued
2013-04-05
PURL
http://purl.flvc.org/fcla/dt/3361091
Subject Headings
Tomatoes, Tomatoes--Nutrition, Lysine, Plant genetic engineering, Genetically modified foods
Format
Document (PDF)
The expression and analysis of a lysine-rich wound-response protein in tomato plants.
Title
The expression and analysis of a lysine-rich wound-response protein in tomato plants.
Creator
Kaplan, Noah, Zhang, Xing-Hai, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
Understanding the genetic regulation of the response to wounding and wound healing in fruiting plants is imperative to maintaining agricultural sustainability, preserving the quality of food supplies, and ensuring the economic viability of agriculture. Many genes are known to be induced by wounding, providing both structural repair and defense. The KED gene in tobacco (Nicotiana tabacum) has been shown to be induced by wounding. We have identified its homologue gene in tomato (Solanum...
Show moreUnderstanding the genetic regulation of the response to wounding and wound healing in fruiting plants is imperative to maintaining agricultural sustainability, preserving the quality of food supplies, and ensuring the economic viability of agriculture. Many genes are known to be induced by wounding, providing both structural repair and defense. The KED gene in tobacco (Nicotiana tabacum) has been shown to be induced by wounding. We have identified its homologue gene in tomato (Solanum lycopersicum) that we named SlKED. We have analyzed gene expression pattern of SlKED through tomato growth and development and in response to wounding as well as hormonal and inhibitor treatments. We found that the plant hormone ethylene played a major role in the expression of SlKED. To further identify evidence for physiological and transductional functions of KED and SlKED, the tobacco KED gene was introduced to tomato and overexpressed by the fruit tissue-active PUN1 promoter from pepper (Capsicum annuum,). The expression of this gene was compared to the expression of the native SlKED gene and other known wound response genes in both the wild-type and transgenic tomato plants. The upregulation of the native SlKED gene by wounding was significantly muted in the tobacco KED-expressing transgenic plants. The expression of other genes known to be associated with wound response transduction pathways was also altered. Our studies implicate the KED gene in defense mechanisms for mechanical stress in tomato plants.
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Date Issued
2016
PURL
http://purl.flvc.org/fau/fd/FA00004773, http://purl.flvc.org/fau/fd/FA00004773
Subject Headings
Wound healing., Wounds and injuries--Genetic aspects., Plant gene expression., Plant genetic regulation., Nanostructured materials--Physiological effect., Biomedical engineering.
Format
Document (PDF)
Nitrate Use Efficiency In Tobacco Plants Constitutively Expressing A Maize Nitrate Transporter ZmNRT2.1
Title
Nitrate Use Efficiency In Tobacco Plants Constitutively Expressing A Maize Nitrate Transporter ZmNRT2.1.
Creator
Cruz, Jessica, Zhang, Xing-Hai, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
The NRT2 (high affinity nitrate transporter 2) family is a part of the iHATS (inducible high affinity system) that studies have shown is responsible for the influx of nitrate into the plant cell after provision of nitrate. The ZmNRT2.1 from Zea mays was constitutively expressed in Nicotiana tabacum. To assess how over-expression of this foreign NRT2.1 affects nitrate influx by plants, nitrate content in leaf and root tissue, gene expression, and vegetal growth were analyzed in media with...
Show moreThe NRT2 (high affinity nitrate transporter 2) family is a part of the iHATS (inducible high affinity system) that studies have shown is responsible for the influx of nitrate into the plant cell after provision of nitrate. The ZmNRT2.1 from Zea mays was constitutively expressed in Nicotiana tabacum. To assess how over-expression of this foreign NRT2.1 affects nitrate influx by plants, nitrate content in leaf and root tissue, gene expression, and vegetal growth were analyzed in media with deficient or high nitrate concentrations (0.1, 1, or 10 mM). Compared to wild type plants: the transgenic lines had a significantly larger fresh weight in all nitrate conditions; primary root length was significantly longer in the 0.1 and 1 mM nitrate conditions; both the fresh weight and the primary root length were significantly higher when 50 mM NaCl was applied as a stress factor to medias containing 0.1 and 10 mM nitrate.
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Date Issued
2015
PURL
http://purl.flvc.org/fau/fd/FA00004492, http://purl.flvc.org/fau/fd/FA00004492
Subject Headings
Nitrogen--Fixation., Nitrogen-fixing plants--Metabolism., Crops and nitrogen., Field crops--Genetic engineering., Plants--Effect of nitrogen on., Soil microbiology.
Format
Document (PDF)