Current Search: Plant gene expression. (x)
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- Title
- Expression of a Dehydrin from the Polar Plant Cerastium arcticum in Transgenic Tobacco.
- Creator
- Hill, William, Zhang, Xing-Hai, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
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Water scarcity induced by drought, temperature, and salinity has plagued agricultural sustainability in recent years with unprecedented revenue losses, raising concerns for worldwide food security. Recent studies have revealed unique botanical response mechanisms to combat water related stress, namely the expression of proteins known as the dehydrins. Dehydrin proteins have been shown to serve various intracellular protective functions. The gene for a SK5 type dehydrin from the arctic plant...
Show moreWater scarcity induced by drought, temperature, and salinity has plagued agricultural sustainability in recent years with unprecedented revenue losses, raising concerns for worldwide food security. Recent studies have revealed unique botanical response mechanisms to combat water related stress, namely the expression of proteins known as the dehydrins. Dehydrin proteins have been shown to serve various intracellular protective functions. The gene for a SK5 type dehydrin from the arctic plant Cerastium arcticum (CaDHN) was introduced into tobacco plants and water deficit tolerance was evaluated. Plants overexpressing CaDHN displayed improved tolerance to salt stress, but no improvement was observed under drought stress.
Show less - Date Issued
- 2015
- PURL
- http://purl.flvc.org/fau/fd/FA00004505, http://purl.flvc.org/fau/fd/FA00004505
- Subject Headings
- Cerastium., Plant bioitechnology., Plants, Effect of stress on., Crops, Effect of stress on., Plant gene expression., Plant proteins., Recombinant proteins.
- Format
- Document (PDF)
- Title
- Analysis of KED-mediated wound response to biotic stress and mechanical damage in tomato plants (Solanum lycopersicum).
- Creator
- Nifakos, Nicholas, Zhang, Xing-Hai, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
- Abstract/Description
-
Lysine-rich KED was previously identified from wounded tobacco (Nicotiana tabacum) leaves before the alignment of protein sequences between NtKED (Nicotiana tabacum KED) and SlKED (Solanum lycopersicum KED) were discovered to display 55.1% identity. Using previously generated SlKED knockout plants by CRISPR/Cas9, we performed biological assays, to investigate the role of KED in wound response to biotic and abiotic stress. Previous studies implied that the KED gene functions as a role in the...
Show moreLysine-rich KED was previously identified from wounded tobacco (Nicotiana tabacum) leaves before the alignment of protein sequences between NtKED (Nicotiana tabacum KED) and SlKED (Solanum lycopersicum KED) were discovered to display 55.1% identity. Using previously generated SlKED knockout plants by CRISPR/Cas9, we performed biological assays, to investigate the role of KED in wound response to biotic and abiotic stress. Previous studies implied that the KED gene functions as a role in the wound-induced mechanism, as well as suggested that it may also function in the plant defense system against biotic stress and insect herbivory. The results from bioassays using tobacco hornworm (Manduca sexta) have proven inconclusive thus far. Expression of KED is induced not only by mechanical wounding but also by touching such as brushing the leaves, indicating that this gene is sensitive to subtle environmental signal and may be involved in defense response against abiotic stress. To further investigate the KED gene’s role in the plant defense system, biological assays using both specialist and generalist herbivores, transcription analysis using various phytohormone mutant plants, and Evans blue cellular damage assays were performed. Our findings imply that the KED gene does not seem to have a long-term effect on insect herbivory but may have a shortterm anti-feeding effect against insect herbivores. Results from the Evans blue membrane damage assay indicate the KED gene may provide some benefit to mechanically damaged plants in a short-term period post-wounding of leaf tissues. Using the SlKED knockout as genetic tool, we conclude that this gene does not confer resistance to insect herbivores over a long-term but seems to provide a beneficial defense response in the short-term. Our membrane damage assay results also imply that this gene may be involved in membrane stabilization and repair of cellular damage after mechanical wounding.
Show less - Date Issued
- 2022
- PURL
- http://purl.flvc.org/fau/fd/FA00014001
- Subject Headings
- Plant gene expression, Solanum lycopersicum, Tomatoes--Effect of stress on
- Format
- Document (PDF)
- Title
- The expression and analysis of a lysine-rich wound-response protein in tomato plants.
- Creator
- Kaplan, Noah, Zhang, Xing-Hai, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Understanding the genetic regulation of the response to wounding and wound healing in fruiting plants is imperative to maintaining agricultural sustainability, preserving the quality of food supplies, and ensuring the economic viability of agriculture. Many genes are known to be induced by wounding, providing both structural repair and defense. The KED gene in tobacco (Nicotiana tabacum) has been shown to be induced by wounding. We have identified its homologue gene in tomato (Solanum...
Show moreUnderstanding the genetic regulation of the response to wounding and wound healing in fruiting plants is imperative to maintaining agricultural sustainability, preserving the quality of food supplies, and ensuring the economic viability of agriculture. Many genes are known to be induced by wounding, providing both structural repair and defense. The KED gene in tobacco (Nicotiana tabacum) has been shown to be induced by wounding. We have identified its homologue gene in tomato (Solanum lycopersicum) that we named SlKED. We have analyzed gene expression pattern of SlKED through tomato growth and development and in response to wounding as well as hormonal and inhibitor treatments. We found that the plant hormone ethylene played a major role in the expression of SlKED. To further identify evidence for physiological and transductional functions of KED and SlKED, the tobacco KED gene was introduced to tomato and overexpressed by the fruit tissue-active PUN1 promoter from pepper (Capsicum annuum,). The expression of this gene was compared to the expression of the native SlKED gene and other known wound response genes in both the wild-type and transgenic tomato plants. The upregulation of the native SlKED gene by wounding was significantly muted in the tobacco KED-expressing transgenic plants. The expression of other genes known to be associated with wound response transduction pathways was also altered. Our studies implicate the KED gene in defense mechanisms for mechanical stress in tomato plants.
Show less - Date Issued
- 2016
- PURL
- http://purl.flvc.org/fau/fd/FA00004773, http://purl.flvc.org/fau/fd/FA00004773
- Subject Headings
- Wound healing., Wounds and injuries--Genetic aspects., Plant gene expression., Plant genetic regulation., Nanostructured materials--Physiological effect., Biomedical engineering.
- Format
- Document (PDF)