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Does methionine sulfoxide reductase have a role in maintaining adequate dopamine levels in drosophila melanogaster?
Title
Does methionine sulfoxide reductase have a role in maintaining adequate dopamine levels in drosophila melanogaster?.
Creator
Hernandez, Caesar, Binninger, David, Graduate College
Date Issued
2013-04-12
PURL
http://purl.flvc.org/fcla/dt/3361311
Subject Headings
Drosophila melanogaster, Methionine Sulfoxide Reductases, Dopamine
Format
Document (PDF)
Alternative Biological Roles of Methionine Sulfoxide Reductases in Drosophila melanogaster
Title
Alternative Biological Roles of Methionine Sulfoxide Reductases in Drosophila melanogaster.
Creator
Wilson, Kelsey, Binninger, David, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
The oxidation of methionine (Met) into methionine sulfoxide (met-(o)) leads to deleterious modifications to a variety of cellular constituents. These deleterious alterations can be reversed by enzymes known as methionine sulfoxide reductases (Msr). The Msr (MsrA and MsrB) family of enzymes have been studied extensively for their biological roles in reducing oxidized Met residues back into functional Met. A wide range of studies have focused on Msr both in vivo and in vitro using a variety of...
Show moreThe oxidation of methionine (Met) into methionine sulfoxide (met-(o)) leads to deleterious modifications to a variety of cellular constituents. These deleterious alterations can be reversed by enzymes known as methionine sulfoxide reductases (Msr). The Msr (MsrA and MsrB) family of enzymes have been studied extensively for their biological roles in reducing oxidized Met residues back into functional Met. A wide range of studies have focused on Msr both in vivo and in vitro using a variety of model organisms. More specifically, studies have noted numerous processes affected by the overexpression, under expression, and silencing of MsrA and MsrB. Collectively, the results of these studies have shown that Msr is involved in lifespan and the management of oxidative stress. More recent evidence is emerging that supports existing biological functions of Msr and theorizes the involvement of Msr in numerous biological pathways.
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Date Issued
2018
PURL
http://purl.flvc.org/fau/fd/FA00005980
Subject Headings
Drosophila melanogaster, Methionine Sulfoxide Reductases, Oxidative stress
Format
Document (PDF)
Effects of Serotonin Modulation on Methionine Sulfoxide Reductase Deficient Drosophila melanogaster
Title
Effects of Serotonin Modulation on Methionine Sulfoxide Reductase Deficient Drosophila melanogaster.
Creator
Hamadeh, Ali, Binninger, David, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description
Methionine sulfoxide reductase (MSR) is an important antioxidant to help mitigate oxidative stress that contributes to age-associated neurodegenerative diseases, such as Alzheimer’s Disease and Parkinson’s Disease. In MSR deficient Drosophila melanogaster (fruit flies), larvae show a developmental delay like that seen when wild-type larvae are reared on nutrient deficit culture medium. These investigators further showed that serotonin levels were depressed in these nutrient deficient larvae....
Show moreMethionine sulfoxide reductase (MSR) is an important antioxidant to help mitigate oxidative stress that contributes to age-associated neurodegenerative diseases, such as Alzheimer’s Disease and Parkinson’s Disease. In MSR deficient Drosophila melanogaster (fruit flies), larvae show a developmental delay like that seen when wild-type larvae are reared on nutrient deficit culture medium. These investigators further showed that serotonin levels were depressed in these nutrient deficient larvae. The overarching aim of this study was to better understand the role of serotonin in MSR regulated physiology. Supplementing food with serotonin partially rescued the slower mouth hook movements (MHM) observed in the MSR-deficient flies. However, supplementation with serotonin altering drugs that cross the blood brain barrier (5-hydroxytryptophan, fluoxetine, or paravi chlorophenylalanine) did not rescue MHM and caused impairments to the growth of larvae during development. This study indicates that serotonin regulates feeding behavior partially through the regulation of MSR production but acts independently to regulate development.
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Date Issued
2021
PURL
http://purl.flvc.org/fau/fd/FA00013761
Subject Headings
Drosophila melanogaster, Methionine sulfoxide reductase, Serotonin
Format
Document (PDF)
Role and Regulation of Methionine Sulfoxide Reductase (Msr) in a model of oxidative stress tolerance: Trachemys scripta
Title
Role and Regulation of Methionine Sulfoxide Reductase (Msr) in a model of oxidative stress tolerance: Trachemys scripta.
Creator
Reiterer, Melissa, Milton, Sarah, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
The detrimental effects of oxidative stress caused by the accumulation of Reactive Oxygen Species (ROS) have been acknowledged as major factors in aging, senescence and several neurodegenerative diseases and conditions such as Parkinson’s disease and stroke (ischemia/reperfusion). Mammalian models are extremely susceptible to these stresses that follow the restoration of oxygen after anoxia; however, some organisms including the freshwater turtle Trachemys scripta can withstand several bouts...
Show moreThe detrimental effects of oxidative stress caused by the accumulation of Reactive Oxygen Species (ROS) have been acknowledged as major factors in aging, senescence and several neurodegenerative diseases and conditions such as Parkinson’s disease and stroke (ischemia/reperfusion). Mammalian models are extremely susceptible to these stresses that follow the restoration of oxygen after anoxia; however, some organisms including the freshwater turtle Trachemys scripta can withstand several bouts of anoxia and repeated reoxygenation without any apparent pathology. T. scripta thus provides us with an alternate vertebrate model in which we can investigate physiological mechanisms of neuroprotection without the damaging effects that come with oxidative stress. The major objective of this study was to investigate the protective mechanisms in the turtle brain under conditions of anoxia and oxidative stress. Specifically, the focus is on the Methionine Sulfoxide Reductase system (Msr), an antioxidant and cellular repair system, and how it is regulated to protect the brain against such stressors. Previous studies in my lab have demonstrated that Msr mRNA and protein levels are differentially upregulated during anoxia and reoxygenation. To investigate the regulation of Msr, FOXO3a was directly induced by transfecting a human FOXO3a plasmid into turtle brain cell cultures, as FOXO3a has been shown to regulate MsrA levels in other animal models. Pharmacological manipulation of FOXO3a was also performed using the green tea extract Epigallocatechin gallate (EGCG) as it has been shown to increase expression of FOXO3a during oxidative stress conditions in other models. I found that an induction of human FOXO3a increased FOXO3a levels and showed protection against cell death during oxidative stress. Furthermore, treatment of cells with EGCG increased expression of FOXO3a only when the cells were exposed to oxidative stress and decreased cell death. Induction of FOXO3a and EGCG treatment did not increase MsrA levels, however MsrB3 levels were upregulated under both treatments but only in the presence of oxidative stress. These results suggest that MsrA and MsrB3 protect the cells from oxidative stress damage through different molecular pathways and that EGCG may be a therapeutic target to treat diseases related to damage by oxidative stress.
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Date Issued
2018
PURL
http://purl.flvc.org/fau/fd/FA00013154
Subject Headings
Methionine Sulfoxide Reductases, Oxidative Stress, Trachemys scripta
Format
Document (PDF)
The Effects of MsrA and MsrB in Anoxia Tolerance in Aging Drosophila melanogaster
Title
The Effects of MsrA and MsrB in Anoxia Tolerance in Aging Drosophila melanogaster.
Creator
Suthakaran, Nirthieca, Binninger, David, Florida Atlantic University, Charles E. Schmidt College of Medicine, Department of Biomedical Sciences
Abstract/Description
Drosophila melanogaster tolerates several hours of anoxia (the absence of oxygen) by entering a protective coma. A burst of reactive oxygen species (ROS) is produced when oxygen is reintroduced to the cells. ROS causes oxidative damage to critical cellular molecules, which contribute to aging and development of certain agerelated conditions. The amino acid, methionine, is susceptible to oxidation, although this damage can be reversed by methionine sulfoxide reductases (Msr). This project...
Show moreDrosophila melanogaster tolerates several hours of anoxia (the absence of oxygen) by entering a protective coma. A burst of reactive oxygen species (ROS) is produced when oxygen is reintroduced to the cells. ROS causes oxidative damage to critical cellular molecules, which contribute to aging and development of certain agerelated conditions. The amino acid, methionine, is susceptible to oxidation, although this damage can be reversed by methionine sulfoxide reductases (Msr). This project investigates the effect of Msr-deficiency on anoxia tolerance in Drosophila throughout the lifespan of the animal. The data show that the time for recovery from the protective comma as well as the survival of the animals lacking any Msr activity depends on how quickly the coma is induced by the anoxic conditions. Insight into the roles(s) of Msr genes under anoxic stress can lead us to a path of designing therapeutic drugs around these genes in relation to stroke.
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Date Issued
2018
PURL
http://purl.flvc.org/fau/fd/FA00013046
Subject Headings
Drosophila melanogaster, Methionine Sulfoxide Reductases, Anoxia, Aging, Oxidative stress
Format
Document (PDF)
Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells
Title
Identification of a truncated form of methionine sulfoxide reductase a expressed in mouse embryonic stem cells.
Creator
Jia, Pingping, Zhang, Chi, Jia, Yuanyuan, Webster, Keith A., Huang, Xupei, Kochegarov, Andrei A., Lemanski, Sharon L., Lemanski, Larry F.
Date Issued
2011-06-22
PURL
http://purl.flvc.org/fcla/dt/3327268
Subject Headings
Cell nucleus -- metabolism, Cloning, Molecular, Cytosol --metabolism, Embryonic Stem Cells --metabolism, Methionine --metabolism, Methionine Sulfoxide Reductases, Methionine Sulfoxide Reductases --metabolism, Methionine Sulfoxide Reductases --genetics, Mitochondria --metabolism, Molecular Sequence Data, Oxidation --Reduction, Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Methionine
Format
Document (PDF)