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The origin of cortical vesicles and their role in egg envelope formation in the "spiny" eggs of a calanoid copepod, Centropages velificatus
Title
The origin of cortical vesicles and their role in egg envelope formation in the "spiny" eggs of a calanoid copepod, Centropages velificatus.
Creator
Blades-Eckelbarger, Pamela I., Marcus, Nancy H., Harbor Branch Oceanographic Institute
Date Issued
1992
PURL
http://purl.flvc.org/FCLA/DT/3174229
Subject Headings
Copepoda, Membranes (Biology), Eggs, Spawning, Oocytes
Format
Document (PDF)
Investigation of talin head-tail interactions
Title
Investigation of talin head-tail interactions.
Creator
Butyn, Amber., Harriet L. Wilkes Honors College
Abstract/Description
Talin is a ubiquitous, high-molecular-weight, flexible protein that plays a critical role in focal adhesions by activating, as well as connecting, integrins to the actin cytoskeleton. Talin's inactive auto-inhibitory state is speculated to be one of its modes of regulation inside the cell and is achieved through its head-tail interactions. In order to decipher the stability of this interaction, the head domain (residues 206-405) was cloned into a modified pET28m vector while the tail domains ...
Show moreTalin is a ubiquitous, high-molecular-weight, flexible protein that plays a critical role in focal adhesions by activating, as well as connecting, integrins to the actin cytoskeleton. Talin's inactive auto-inhibitory state is speculated to be one of its modes of regulation inside the cell and is achieved through its head-tail interactions. In order to decipher the stability of this interaction, the head domain (residues 206-405) was cloned into a modified pET28m vector while the tail domains (residues 1654-2344 and 2225-2344) were cloned into the pET32a vector to obtain octa-histidine tagged and un-tagged peptide, respectively. Neither co-expression nor pull-down using the His-tagged head domain was successful in purifying a stable head-tail complex. Our results indicate rather weak interactions between the talin head and rod domains and hence, under our experimental conditions, do not lead to a stable auto-inhibitory complex that can be purified for further studies.
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Date Issued
2009
PURL
http://purl.flvc.org/FAU/209984
Subject Headings
Membrane proteins, Structure-activity relationships, Cell membranes, Physiology, Molecular biology, Cell interaction, Developmental cytology
Format
Document (PDF)
Construction of mitochondrion-targeted telomerase for analysis in Saccharomyces cerevisiae
Title
Construction of mitochondrion-targeted telomerase for analysis in Saccharomyces cerevisiae.
Creator
Martin, Ricardo., Harriet L. Wilkes Honors College
Abstract/Description
Telomerase is associated with telomere production and nDNA protection. However, studies by Santos et al. have demonstrated that human telomerase has a mitochondrial entry sequence and in the presence of hydrogen peroxide it has been found inside the mitochondrion and may cause mitochondrial DNA mutations. Saccharomyces cerevisiae contains telomerase, but it does not have the mitochondrial entry sequence. To determine if the presence of telomerase in the mitochondria can induce mutations an...
Show moreTelomerase is associated with telomere production and nDNA protection. However, studies by Santos et al. have demonstrated that human telomerase has a mitochondrial entry sequence and in the presence of hydrogen peroxide it has been found inside the mitochondrion and may cause mitochondrial DNA mutations. Saccharomyces cerevisiae contains telomerase, but it does not have the mitochondrial entry sequence. To determine if the presence of telomerase in the mitochondria can induce mutations an experiment was developed in which a mitochondrion entry sequence would be fused to the S. cerevisiae telomerase enzyme. This fusion could then be screened in S. cerevisiae with an ade2 mutation for a simple color assay of mitochondrial activity. To date, no successful transformant has been identified. The frequency of incorrect ligations has been recognized and may indicate that the desired fusion is lethal to E. coli cells.
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Date Issued
2009
PURL
http://purl.flvc.org/FAU/209994
Subject Headings
Cell membranes, Formation, Mitochondrial DNA, Mutation (Biology), Cell metabolism
Format
Document (PDF)
Improving In Vivo Two Photon Microscopy Without Adaptive Optics
Title
Improving In Vivo Two Photon Microscopy Without Adaptive Optics.
Creator
Estrada, Gerardo, Beetle, Christopher, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Physics
Abstract/Description
Two photon microscopy is one of the fastest growing methods of in-vivo imaging of the brain. It has the capability of imaging structures on the scale of 1μm. At this scale the wavelength of the imaging field (usually near infra-red), is comparable to the size of the structures being imaged, which makes the use of ray optics invalid. A better understanding is needed to predict the result of introducing different media into the light path. We use Wolf's integral, which is capable of fulfilling...
Show moreTwo photon microscopy is one of the fastest growing methods of in-vivo imaging of the brain. It has the capability of imaging structures on the scale of 1μm. At this scale the wavelength of the imaging field (usually near infra-red), is comparable to the size of the structures being imaged, which makes the use of ray optics invalid. A better understanding is needed to predict the result of introducing different media into the light path. We use Wolf's integral, which is capable of fulfilling these needs without the shortcomings of ray optics. We predict the effects of aberrating media introduced into the light path like glass cover-slips and then correct the aberration using the same method. We also create a method to predict aberrations when the interfaces of the media in the light-path are not aligned with the propagation direction of the wavefront.
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Date Issued
2015
PURL
http://purl.flvc.org/fau/fd/FA00004495
Subject Headings
Cellular signal transmission -- Measurement, Image analysis, Imaging systems in medicine, Membranes (Biology) -- Imaging, Neurons -- Imaging, Optics, Adaptive
Format
Document (PDF)
The effects of Toll-like receptor (TLR) agonists on human nicDC-NK mediated memory/effector T-cell development
Title
The effects of Toll-like receptor (TLR) agonists on human nicDC-NK mediated memory/effector T-cell development.
Creator
Tamjidi, Saba, Nouri-Shirazi, Mahyar, Florida Atlantic University, Charles E. Schmidt College of Medicine, Department of Biomedical Science
Abstract/Description
There is compelling evidence that smokers are less responsive to vaccination. We reported that both therapeutic and prophylactic vaccines fail to protect and cure animals from disease due to negative effects of nicotine on DCs’ ability to generate effector T cells. We have been investigating whether vaccine formulated with TLR agonist(s) could potentially overcome the immunosuppressive effects of nicotine on human DC-NK cross-talk essential for effector T cell generation. Monocyte-derived DCs...
Show moreThere is compelling evidence that smokers are less responsive to vaccination. We reported that both therapeutic and prophylactic vaccines fail to protect and cure animals from disease due to negative effects of nicotine on DCs’ ability to generate effector T cells. We have been investigating whether vaccine formulated with TLR agonist(s) could potentially overcome the immunosuppressive effects of nicotine on human DC-NK cross-talk essential for effector T cell generation. Monocyte-derived DCs and nicDCs were stimulated with individual and combined TLR agonists prior to co-culture with purified T cells. The phenotypes and cytokine profiles of T cell were assessed using Flow Cytometry and ELISA, respectively. We found nicDCs cultured with TLR-8/7 alone or in combination with TLR-3 produce quantitatively and qualitatively similar IFN-γ producing effector T cells when compared to control DCs. Our data suggest that the addition of appropriate TLR agonist to vaccine formulation could potentially overcome the immunosuppression seen in smokers, thereby containing the spread of infectious disease to vulnerable population
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Date Issued
2015
PURL
http://purl.flvc.org/fau/fd/FA00004469, http://purl.flvc.org/fau/fd/FA00004469
Subject Headings
Cell membranes, Cell receptors, Evidence based medicine, Immune system, Molecular biology, T cells -- Receptors, Tobacco -- Physiological effects
Format
Document (PDF)