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Characterization of the MHC II B of the bald eagle
Title
Characterization of the MHC II B of the bald eagle.
Creator
Smith, Andrew., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
The Major Histocompatibility Complex class II B (MHC II B) gene encodes a protein that is part of the adaptive immune system and critical for the non-self recognition ability of immune cells. This gene has been characterized in the Bald Eagle, ten unique alleles were found in two subpopulations at the geographic extremes of the range margins. Geographic genetic variation is suggested by the presence of population specific alleles. The results showed considerable divergence of groups of Bald...
Show moreThe Major Histocompatibility Complex class II B (MHC II B) gene encodes a protein that is part of the adaptive immune system and critical for the non-self recognition ability of immune cells. This gene has been characterized in the Bald Eagle, ten unique alleles were found in two subpopulations at the geographic extremes of the range margins. Geographic genetic variation is suggested by the presence of population specific alleles. The results showed considerable divergence of groups of Bald Eagle alleles when compared to alleles from other birds of prey. Particular codons within the exon II show signs of balancing selection driving the evolution of the MHC II B. Transcription data showed statistically significant differential expression of alleles. This can be interpreted as meaning a particular locus is being preferentially expressed in blood. The analysis of the polymorphism of this adaptive marker may aid managers of wildlife during this age of global climate change and the biodiversity crisis.
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Date Issued
2010
PURL
http://purl.flvc.org/FAU/2979375
Subject Headings
Major histocompatibility complex, Cellular signal transduction, Immunogenetics, Genetic polymorphisms
Format
Document (PDF)
Polymorphism and selection at exon 2 of the class II beta chain of the major histocompatibility complex in Forpus passerinus
Title
Polymorphism and selection at exon 2 of the class II beta chain of the major histocompatibility complex in Forpus passerinus.
Creator
Walbroehl, Jaclyn M., Florida Atlantic University, Hughes, Colin
Abstract/Description
Female mate choice has been proposed to be a major force acting to maintain the polymorphism of the MHC, the most genetically variable genes known. I genotyped seventy-two individuals from a free-living population of the parrot, Forpus passerinus, in order to determine whether disassortative mating is the mode of selection acting at this locus. Both the high rate of nonsynonymous amino acid changes at the peptide binding regions of exon 2 of the class II beta gene and the unprecedented number...
Show moreFemale mate choice has been proposed to be a major force acting to maintain the polymorphism of the MHC, the most genetically variable genes known. I genotyped seventy-two individuals from a free-living population of the parrot, Forpus passerinus, in order to determine whether disassortative mating is the mode of selection acting at this locus. Both the high rate of nonsynonymous amino acid changes at the peptide binding regions of exon 2 of the class II beta gene and the unprecedented number of alleles found in this population provided strong evidence for the operation of selection. Despite this, a chi-square test revealed no evidence of disassortative mating for this sample, X2=0.569, n.s. I propose that natural selection, not sexual selection, must be operating at this locus to maintain the degree of variation found in 270 base-pairs of this gene.
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Date Issued
2006
PURL
http://purl.flvc.org/fcla/dt/13405
Subject Headings
Immunogenetics, Genetic polymorphisms, Major histocompatibility complex--Genetic aspects
Format
Document (PDF)
Diversity and selection in the major histocompatibility complex
Title
Diversity and selection in the major histocompatibility complex: DQA and immune function in IRL and Atlantic bottlenose dolphins (Tursiops truncatus).
Creator
Ferrer, Tatiana., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description
The Major Histocompatibility Complex (MHC) encodes proteins critical to the vertebrate immune response; therefore MHC diversity is an indicator of population health. I have (1) Isolated exon 2 of the class II gene DQA in Tursiops truncatus in the North Indian River Lagoon (IRL) (n=17), South IRL (n=29) and adjacent Atlantic waters (n=20), (2) assessed genetic variability between groups, (3) developed a method to genotype individuals, (4) typed 11 unique alleles in 66 individuals, (5) detected...
Show moreThe Major Histocompatibility Complex (MHC) encodes proteins critical to the vertebrate immune response; therefore MHC diversity is an indicator of population health. I have (1) Isolated exon 2 of the class II gene DQA in Tursiops truncatus in the North Indian River Lagoon (IRL) (n=17), South IRL (n=29) and adjacent Atlantic waters (n=20), (2) assessed genetic variability between groups, (3) developed a method to genotype individuals, (4) typed 11 unique alleles in 66 individuals, (5) detected geographic patterns of diversity between estuarine and coastal individuals (FST=0.1255, p<0.05), (6) found evidence of positive selection centered in the binding pockets P1, P6 and P9 of the peptide binding region (w=2.08), (7) found that patterns of polymorphism did not closely match patterns of diversity in neutral markers, (8) performed a pilot study with Orcinus orca. The initial findings highlight the need for further comparative work and suggest that silent mutations are not neutral.
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Date Issued
2013
PURL
http://purl.flvc.org/fcla/dt/3362335
Subject Headings
Major histocompatibility complex, Immunogenetics, Molecular genetics, Endocrine disrupting chemicals, Dolphins, Geographical distribution, Population genetics, Social behavior in animals
Format
Document (PDF)