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Microextraction of total lipid from mesopelagic animals
Title
Microextraction of total lipid from mesopelagic animals.
Creator
Reisenbichler, K. R., Bailey, T. G., Harbor Branch Oceanographic Institute
Date Issued
1991
PURL
http://purl.flvc.org/FCLA/DT/3353868
Subject Headings
Lipids--Analysis, Lipids--Analysis--Technique
Format
Document (PDF)
Annual Fluctuations of Lipid Levels in the Sea Anemone Anthopleura Elegantissima (Brandt, 1835)
Title
Annual Fluctuations of Lipid Levels in the Sea Anemone Anthopleura Elegantissima (Brandt, 1835).
Creator
Jennison, Brian L., Harbor Branch Oceanographic Institute
Date Issued
1979
PURL
http://purl.flvc.org/FCLA/DT/3331808
Subject Headings
Sea anemones, Lipids, Anthopleura
Format
Document (PDF)
Source and fate oflipids in polar gelatinous zooplankton
Title
Source and fate oflipids in polar gelatinous zooplankton.
Creator
Larson, R. J., Harbison, G. R., Harbor Branch Oceanographic Institute
Date Issued
1989
PURL
http://purl.flvc.org/fau/fd/FA00007195
Subject Headings
Zooplankton, Ctenophora, Medusae, Lipids
Format
Document (PDF)
Comparative ultrastructure of lipid storage sites in female Euchaeta marina and Pleuromamma xiphias (Copepoda: Calanoida)
Title
Comparative ultrastructure of lipid storage sites in female Euchaeta marina and Pleuromamma xiphias (Copepoda: Calanoida).
Creator
Blades-Eckelbarger, Pamela I., Harbor Branch Oceanographic Institute
Date Issued
1991
PURL
http://purl.flvc.org/FCLA/DT/3333172
Subject Headings
Copepoda, Calanoida, Pleuromamma xiphias, Ultrastructure (Biology), Lipids
Format
Document (PDF)
Proximate composition of eggs andlarvae of the sand dollar Encope michelini (Agassiz): the advantage of higher investment inplankotrophic eggs
Title
Proximate composition of eggs andlarvae of the sand dollar Encope michelini (Agassiz): the advantage of higher investment inplankotrophic eggs.
Creator
George, Sophie B., Young, Craig M., Fenaux, Lucienne, Harbor Branch Oceanographic Institute
Date Issued
1997
PURL
http://purl.flvc.org/fau/fd/FA00007268
Subject Headings
Sand dollars, Eggs, Larvae, Protein, Lipids
Format
Document (PDF)
Biophysical characterization of bioactive peptide amphiphiles
Title
Biophysical characterization of bioactive peptide amphiphiles.
Creator
Fishel, Ayala, Florida Atlantic University, Fields, Gregg B.
Abstract/Description
In this present work we have examined the biophysical characterization of two peptides. One alpha-helical (SPARC) and the other triple-helical (collagen). We have compared the effect of lipidation on stabilizing of the alpha-helical and triple-helical peptides. For the first peptide, amino acids from the angiogenesis-inducing region of SPARC were incorporated into alpha-helical peptide sequence. A Tyr was placed between the alpha-helical sequence and the peptide to provide a chromophore; Lys...
Show moreIn this present work we have examined the biophysical characterization of two peptides. One alpha-helical (SPARC) and the other triple-helical (collagen). We have compared the effect of lipidation on stabilizing of the alpha-helical and triple-helical peptides. For the first peptide, amino acids from the angiogenesis-inducing region of SPARC were incorporated into alpha-helical peptide sequence. A Tyr was placed between the alpha-helical sequence and the peptide to provide a chromophore; Lys-Ala-Glu-Ile-Glu-Ala-Leu-Lys-Ala-Glu-Ile-Glu-Ala-Leu-Lys-Ala-Tyr-Lys-His-Gly-Lys-NH 2 was the final sequence. For the second peptide, the sequence chosen to mimic the alpha2beta1 integrin binding site in type I collagen was (Gly-Pro-Hyp)4-Gly-Pro-Ala-Gly-Lys-Asp-Gly-Glu-Ala-Gly-Ala-Gln-(Gly-Pro-Hyp) 4-NH2. Next, each peptide was lipidated with a C-16 acid and was biophysically characterized to determine physiological compatibility. Techniques used included circular dichroism spectroscopy, gel electrophoresis, and Fourier transform infrared spectroscopy. Lastly, one of the peptide amphiphiles was examined as a biomaterial modifier.
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Date Issued
2000
PURL
http://purl.flvc.org/fcla/dt/15780
Subject Headings
Peptides--Synthesis, Lipids, Bioactive compounds
Format
Document (PDF)
The correlation between liver dry matter and liver lipid in cultured juvenile red drum fed various diets
Title
The correlation between liver dry matter and liver lipid in cultured juvenile red drum fed various diets.
Creator
Tucker, John W., Jr., Lellis, W. A., Vermeer, Gregory K., Harbor Branch Oceanographic Institute
Date Issued
2001
PURL
http://purl.flvc.org/fau/fd/FA00007276
Subject Headings
Red drum, Sciaenops ocellatus, Liver, Lipids, Fishes--Feeding and feeds
Format
Document (PDF)
The effect of menopausal status on substrate utilization in younger women during submaximal exercise
Title
The effect of menopausal status on substrate utilization in younger women during submaximal exercise.
Creator
Pittinger, Elizabeth Smith, Zoeller, Robert F., Florida Atlantic University, College of Education, Department of Exercise Science and Health Promotion
Abstract/Description
The purpose of this study was to determine if surgically-induced menopause in younger women affects substrate utilization during submaximal exercise while controlling for other potential confounds. METHODS: Thirteen untrained female subjects (33-50 years old) were recruited: oophorectomized (Group O = 5) and premenopausal controls (Group C = 8). Two separate visits included: body composition and maximal treadmill exercise test; followed by substrate utilization via open-circuit spirometry...
Show moreThe purpose of this study was to determine if surgically-induced menopause in younger women affects substrate utilization during submaximal exercise while controlling for other potential confounds. METHODS: Thirteen untrained female subjects (33-50 years old) were recruited: oophorectomized (Group O = 5) and premenopausal controls (Group C = 8). Two separate visits included: body composition and maximal treadmill exercise test; followed by substrate utilization via open-circuit spirometry during 45 minutes of treadmill walking at 50% VO2max. RESULTS: When controlling for multiple variables affecting whole-body substrate utilization (age, VO2max, physical activity, body composition, fasting glucose, menstrual phase and diet), there was no difference in substrate utilization between pre- and postmenopausal women as measured by respiratory exchange ratio (RER) (0.83 ± 0.04 v 0.84 ± 0.03, p=0.47). CONCLUSIONS: Menopausal status does not appear to have an effect on substrate utilization during submaximal exercise in younger women.
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Date Issued
2015
PURL
http://purl.flvc.org/fau/fd/FA00004400, http://purl.flvc.org/fau/fd/FA00004400
Subject Headings
Lipids--Metabolism, Exercise for women--Physiological aspects, Menopause--Physiological aspects, Endocrine gynecology
Format
Document (PDF)
SUPPORTED ERYTHROCYTE MEMBRANES ON PIEZOELECTRIC SENSORS FOR STUDYING THE INTERACTIONS BETWEEN NANOPARTICLES AND SURFACES OF RED BLOOD CELLS
Title
SUPPORTED ERYTHROCYTE MEMBRANES ON PIEZOELECTRIC SENSORS FOR STUDYING THE INTERACTIONS BETWEEN NANOPARTICLES AND SURFACES OF RED BLOOD CELLS.
Creator
Islam, Tanaz, Yi, Peng, Florida Atlantic University, Department of Civil, Environmental and Geomatics Engineering, College of Engineering and Computer Science
Abstract/Description
The Supported red blood cell membrane (SRBCm) was developed on a piezoelectric sensor to study the attachment of nanoparticles to erythrocyte surfaces. A well-dispersed colloidal suspension of fragments of RBCm was prepared from whole blood, and characterized thoroughly using cryogenic transmission electron microscopy, dynamic light scattering, and zeta potential analysis. To develop SRBCm, RBCm fragments were immobilized onthe sensor in a quartz crystal microbalance with dissipation...
Show moreThe Supported red blood cell membrane (SRBCm) was developed on a piezoelectric sensor to study the attachment of nanoparticles to erythrocyte surfaces. A well-dispersed colloidal suspension of fragments of RBCm was prepared from whole blood, and characterized thoroughly using cryogenic transmission electron microscopy, dynamic light scattering, and zeta potential analysis. To develop SRBCm, RBCm fragments were immobilized onthe sensor in a quartz crystal microbalance with dissipation monitoring system. A complete monolayer of flattened fragments of RBCm was formed on the positively charged surface of the piezoelectric sensor in 1 mM NaCl and 0.2 mM NaHCO3 at pH 7.1. The surface morphology of SRBCm was characterized via atomic force microscopy. The even distribution of surface proteins expressed on erythrocytes was found on SRBCm through indirect immunofluorescence microscopy. The attachment efficiencies of model nanoparticles, e.g. hematite nanoparticles and carboxylated polystyrene nanoparticles, on the SRBCm were quantified using a classic methodology. KEYWORDS: Supported erythrocyte membrane, piezoelectric sensor, phospholipid bilayers, nanoparticles
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Date Issued
2019
PURL
http://purl.flvc.org/fau/fd/FA00013383
Subject Headings
Erythrocyte Membrane, Piezoelectric polymer biosensors, Nanoparticles, Phospholipid bilayers, Bilayer lipid membranes
Format
Document (PDF)
Analysis of sphingolipids from biological sources using on-line high performance liquid chromatography with detection by atmospheric pressure chemical ionization and electrospray ionization mass spectrometry
Title
Analysis of sphingolipids from biological sources using on-line high performance liquid chromatography with detection by atmospheric pressure chemical ionization and electrospray ionization mass spectrometry.
Creator
Perry, Richard H., Florida Atlantic University, Byrdwell, William Craig
Abstract/Description
Sphingolipids (SPLs) are important structural components of membranes in some types of cells and are involved in numerous signaling processes. Sphingomyelin (SPM) and dihydrosphingomyelin (DHS) are the two major SPLs in membranes. Very little is known about the molecular species and role of DHS in biological systems. In this work, we employed high performance liquid chromatography with detection by atmospheric pressure chemical ionization and electrospray ionization (ESI) mass spectrometry to...
Show moreSphingolipids (SPLs) are important structural components of membranes in some types of cells and are involved in numerous signaling processes. Sphingomyelin (SPM) and dihydrosphingomyelin (DHS) are the two major SPLs in membranes. Very little is known about the molecular species and role of DHS in biological systems. In this work, we employed high performance liquid chromatography with detection by atmospheric pressure chemical ionization and electrospray ionization (ESI) mass spectrometry to elucidate the SPL composition in biological extracts. No common dietary source of DHS is known to exist. A novel analytical method developed to analyze complex SPL mixtures was used to show that bovine milk contained substantial amounts of DHS. Also, the human lens is the only known system in which DHS is the most abundant SPL. The molecular species of DHS in cataractous lenses has never been reported. It was shown that there was a preference for monounsaturated species of DHS and SPM in all ages and in cataractous lenses. It was also discovered that SPLs were the primary PLs remaining in cataractous lenses. Finally, the formation of sodium adducts and dimers in the ESI source of the ion trap mass spectrometer prevented the accurate quantitative analysis of PLs. A new method was developed to eliminate these undesirable ions.
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Date Issued
2004
PURL
http://purl.flvc.org/fcla/dt/13150
Subject Headings
Lipids--Synthesis, Liquid chromatography, Mass spectrometry, Sphingolipids--Biotechnology, Cell interaction
Format
Document (PDF)
The Effect of a Supportive-Educative Nursing Intervention on Weight Loss and Perceived Self Care in Overweight Women With Metabolic Syndrome
Title
The Effect of a Supportive-Educative Nursing Intervention on Weight Loss and Perceived Self Care in Overweight Women With Metabolic Syndrome.
Creator
Fleck, Laureen, Parker, Marilyn, Florida Atlantic University
Abstract/Description
Metabolic syndrome is a constellation of risk factors that are precursors to diabetes and cardiovascular disease. More women than men are diagnosed with metabolic syndrome. Emphasis on diagnosis of pre-diabetes has resulted in further interest in metabolic syndrome and the need for weight reduction. Advanced practice nurses care for women with overweight and obesity in the primary care setting; however there is a lack of evidence to support the effectiveness of nursing interventions to assist...
Show moreMetabolic syndrome is a constellation of risk factors that are precursors to diabetes and cardiovascular disease. More women than men are diagnosed with metabolic syndrome. Emphasis on diagnosis of pre-diabetes has resulted in further interest in metabolic syndrome and the need for weight reduction. Advanced practice nurses care for women with overweight and obesity in the primary care setting; however there is a lack of evidence to support the effectiveness of nursing interventions to assist women with metabolic syndrome lose weight. Based on Orem's Self-Care Deficit Nursing Theory, this study investigated the use of a specific supportive-educative nursing intervention to assist individuals make healthy lifestyle choices to reduce body weight. A pre-test post-test, two group experimental design was used. It was hypothesized that women given individualized support, education, and guidance about lifestyle modification, which included carbohydrate counting, would lose more weight and have greater self-care ability than women given general support and education. The sample included 51 pre-menopausal women between ages 19 and 55 who had been diagnosed with metabolic syndrome. Body weight and perceived self-care, using the Self-as-Carer inventory, were measured at the onset of the study and again three months later. The two groups did not differ significantly as to weight or perceived self-care at baseline or at conclusion of the study. A repeated measures analysis of variance for weight loss and perception of self care was calculated and the results indicated that there was no significant difference in weight loss (p=.13) or perception of self care (p=.85) between the two groups. The use of a supportive-educative individualized nursing intervention enhanced participant awareness of healthy lifestyle choices. Further research is needed to determine the effectiveness of the intervention with a larger, more ethnically diverse sample and with participants diagnosed with other metabolic disorders.
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Date Issued
2007
PURL
http://purl.flvc.org/fau/fd/FA00000620
Subject Headings
Metabolic syndrome, Obesity--Treatment, Lipids--Metabolism--Disorders--Treatment, Integrated delivery of health care, Self-care, Health, Nurse and patient
Format
Document (PDF)