Current Search: In vitro (x)
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Title
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ESTABLISHING A SPONGE HYBRIDOMA FOR THE IN VITRO PRODUCTION OF A MARINE NATURAL PRODUCT.
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Creator
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Dougan, Cassady, Pomponi, Shirley A., Florida Atlantic University, Department of Marine Science and Oceanography, Charles E. Schmidt College of Science
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Abstract/Description
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Marine sponges are one of the most prolific sources of chemical compounds with pharmaceutical importance. To establish a supply of such compounds large enough for clinical development, in vitro production methodology was investigated. Since all sponge cells do not divide in culture, it was hypothesized that the fusion of rapidly dividing cells of a sponge that does not produce any compounds of interest with cells of a nondividing but compound-producing sponge would result in a hybridoma that...
Show moreMarine sponges are one of the most prolific sources of chemical compounds with pharmaceutical importance. To establish a supply of such compounds large enough for clinical development, in vitro production methodology was investigated. Since all sponge cells do not divide in culture, it was hypothesized that the fusion of rapidly dividing cells of a sponge that does not produce any compounds of interest with cells of a nondividing but compound-producing sponge would result in a hybridoma that produces the compound of interest. In this study, hybridomas have been produced with cells of two marine sponges, Axinella corrugata, which produces the antitumor compound stevensine, and Geodia neptuni, which divides rapidly in a nutrient medium optimized for sponge cell culture. Successful hybridization and subsequent cell division and in vitro stevensine production may unlock the potential for sustainable mass production of other sponge-derived compounds.
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Date Issued
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2022
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PURL
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http://purl.flvc.org/fau/fd/FA00014024
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Subject Headings
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Marine natural products, Sponges, Hybridomas, In vitro
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Format
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Document (PDF)
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Title
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Semisynthetic Analogues of the Microtubule‐Stabilizing Agent Discodermolide: Preparation and Biological Activity.
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Creator
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Gunasekera, Sarath P., Longley, Ross E., Isbrucker, Richard A., Harbor Branch Oceanographic Institute
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Date Issued
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2002
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PURL
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http://purl.flvc.org/FCLA/DT/3164101
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Subject Headings
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Sponges, Polyketides, Spectrum analysis, Polymerization, Toxicity testing --In vitro
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Format
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Document (PDF)
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Title
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Characterization and optimization of in vitro assay conditions for (1,3)β-glucan synthase activity from Aspergillus fumigatus and Candida albicans for enzyme inhibition screening.
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Creator
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Wood, R. L., Miller, T. K., Wright, Amy E., McCarthy, Peter J., Taft, C. S., Pomponi, Shirley A., Selitrennikoff, Claude, Harbor Branch Oceanographic Institute
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Date Issued
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1998
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PURL
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http://purl.flvc.org/FCLA/DT/3351974
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Subject Headings
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Aspergillus fumigatus, Candida albicans, Glucosyltransferases, In vitro, Glucan synthase, Enzyme inhibitors
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Format
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Document (PDF)
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Title
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Secobatzellines A and B, two new enzyme inhibitors from a deep-water Caribbean sponge of the genus Batzella.
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Creator
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Gunasekera, Sarath P., McCarthy, Peter J., Longley, Ross E., Pomponi, Shirley A., Wright, Amy E.
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Date Issued
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1999
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PURL
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http://purl.flvc.org/FCLA/DT/3172682
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Subject Headings
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Sponges --Caribbean Sea, Marine natural products, Toxicity testing --In vitro, Phosphatases, Peptidase
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Format
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Document (PDF)
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Title
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In vitro production of marine-derived antitumor compounds.
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Creator
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Pomponi, Shirley A., Willoughby, Robin, Wright, Amy E., Pecorella, C., Sennett, Susan H., Lopez, Jose V., Samples, Gail A.
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Date Issued
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1998
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PURL
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http://purl.flvc.org/fau/fd/FA00007238
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Subject Headings
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Antineoplastic agents, Bioactive compounds, Marine natural products, In vitro, Antitumor agents
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Format
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Document (PDF)
