Current Search: Freshwater algae (x)
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- Title
- Effects of Carbon Dioxide Levels on Growth and Pigments of Freshwater Algae.
- Creator
- Bermudez, Jeanne, Louda, J. W., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
-
This project was designed to investigate the effects of carbon dioxide (CO2) levels on the growth and pigment ratios (chemotaxonomy) of freshwater algal species typical to the south Florida surface waters. Green algae, diatoms, and cyanobacteria were cultured under 400 or 800 ppm CO3 in air for several weeks. Growth monitoring used a cell counter, hemocytometer, and chlorophyll fluorescence. Pigments were analyzed using HPLC-PDA. Experiments with certified CO2 concentrations (400, 600, 800,...
Show moreThis project was designed to investigate the effects of carbon dioxide (CO2) levels on the growth and pigment ratios (chemotaxonomy) of freshwater algal species typical to the south Florida surface waters. Green algae, diatoms, and cyanobacteria were cultured under 400 or 800 ppm CO3 in air for several weeks. Growth monitoring used a cell counter, hemocytometer, and chlorophyll fluorescence. Pigments were analyzed using HPLC-PDA. Experiments with certified CO2 concentrations (400, 600, 800, 1200 ppm) were conducted with helium degassed ultrapure water and each of three culture media. Theoretical and experimental pH values with water matched exactly. However, each culture media proved to exhibit significant buffer capacity. Cell growth monitoring was problematic except for the cyanobacterium Microcystis aeruginosa. That species responded to increased CO2 (800 ppm) with increased growth rates as predicted. The other species gave erratic results mainly due to difficulties in obtaining valid consistent cell counts.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FA00013170
- Subject Headings
- Freshwater algae--Growth, Chemotaxonomy, Carbon dioxide, Pigments (Biology)
- Format
- Document (PDF)
- Title
- Comparison of chemotaxonomic methods for the determination of periphyton community composition.
- Creator
- Browne, Jamie L., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
- Abstract/Description
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Pigment-based chemotaxonomy uses relative amounts of photosynthetic pigments (biomarkers) within algae samples to determine the algal class composition of each sample. Chemotaxonomy has been applied successfully to phytoplankton communities, but its efficacy for periphyton has not yet been established. This study examined the ability of simultaneous linear equations (SLE), CHEMTAX, and the Bayesian Compositional Estimator (BCE) to determine algal class composition in Florida Everglades...
Show morePigment-based chemotaxonomy uses relative amounts of photosynthetic pigments (biomarkers) within algae samples to determine the algal class composition of each sample. Chemotaxonomy has been applied successfully to phytoplankton communities, but its efficacy for periphyton has not yet been established. This study examined the ability of simultaneous linear equations (SLE), CHEMTAX, and the Bayesian Compositional Estimator (BCE) to determine algal class composition in Florida Everglades periphyton. The methods were applied to artificial datasets, mixed lab cultures of known composition, and Everglades periphyton samples for which microscopic biovolume data was available. All methods were able to return accurate sample compositions for artificial data and mixed lab cultures. Correlation between pigment methods and microscopic results for natural periphyton samples was poor. SLE and CHEMTAX returned similar results for all samples while BCE performed less well.
Show less - Date Issued
- 2010
- PURL
- http://purl.flvc.org/FAU/2100582
- Subject Headings
- Water quality biological assessment, Periphyton, Water, Phosphorus content, Freshwater algae
- Format
- Document (PDF)
- Title
- Pigment analysis of benthic and pelagic algae in Lake Okeechobee, Florida.
- Creator
- Winfree, Nancy, Florida Atlantic University, Baker, Earl W.
- Abstract/Description
-
Water samples from Lake Okeechobee, Florida have been analyzed in order to relate pigment fingerprints to temporal and spatial variations. Chemical taxonomic identification of predominant photoautotrophs was based on pigment profiles evolved from literature reports and analysis of specific cultures. Carotenoids and chlorophylls were analyzed using high performance liquid chromatography (HPLC) and ultra-violet visible spectra obtained with a photo diode array detector (PDA). Pigments were...
Show moreWater samples from Lake Okeechobee, Florida have been analyzed in order to relate pigment fingerprints to temporal and spatial variations. Chemical taxonomic identification of predominant photoautotrophs was based on pigment profiles evolved from literature reports and analysis of specific cultures. Carotenoids and chlorophylls were analyzed using high performance liquid chromatography (HPLC) and ultra-violet visible spectra obtained with a photo diode array detector (PDA). Pigments were identified by coincident matches of retention time and UV/Visible spectral properties. Chemical taxonomic identification was determined by comparison with known photoautotrophic pigment 'fingerprints'. Experimental results show pigment arrays indicative of chromophytes, chlorophytes and cyanophytes as well as photoautotrophic bacteria present in Lake Okeechobee. The lake was also found to be heterogeneous and dynamic in terms of pigment composition. Major pigment groups changed as a function of location in the lake and as a function of season. A greater pigment diversity was found to exist in the benthos due to the presence of degradation products. HPLC-PDA pigment analysis for chemotaxonomic typing of photoautotrophic communities is attainable and can be used as a rapid viable alternative to microscopic examination of plankton and epiphyte samples. Also, taxonomic typing of organic matter and photoautotrophic communities of sediments, including bacterial populations, can be accomplished using this method.
Show less - Date Issued
- 1996
- PURL
- http://purl.flvc.org/fcla/dt/15261
- Subject Headings
- Plant pigments--Analysis, Photosynthetic pigments--Analysis, Freshwater algae, High performance liquid chromatography, Okeechobee, Lake (Fla)
- Format
- Document (PDF)
- Title
- The effect of canopy shading on relative primary productivity of periphyton in Water Conservation Area 2-A, Everglades.
- Creator
- Brandenburg, Mark Eric., Florida Atlantic University, Marsh, G. Alex
- Abstract/Description
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Differences in relative primary productivity of periphyton were determined for three light regimes: open slough (control), sawgrass canopy, and cattail canopy. The center of Water Conservation Area 2-A, Everglades was chosen as the study site because it is relatively unaffected by nutrient runoff. Periphyton productivity was determined by the light and dark bottle method, and biomass was recorded as ash-free dry weight. Statistically significant correlations were found between periphyton...
Show moreDifferences in relative primary productivity of periphyton were determined for three light regimes: open slough (control), sawgrass canopy, and cattail canopy. The center of Water Conservation Area 2-A, Everglades was chosen as the study site because it is relatively unaffected by nutrient runoff. Periphyton productivity was determined by the light and dark bottle method, and biomass was recorded as ash-free dry weight. Statistically significant correlations were found between periphyton biomass and water temperature and between organic content and water temperature. Mean photosynthetically active radiation reaching the surface of the water under sawgrass and cattail canopy was 45% $\pm$ 3% and 11% $\pm$ 1% respectively. Relative primary productivity of periphyton was lower under cattail canopy than under sawgrass canopy due to increased shading of cattail. This reduction of periphyton biomass and primary productivity can be considered an indirect effect of nutrient enrichment from the Everglades Agricultural Area.
Show less - Date Issued
- 1996
- PURL
- http://purl.flvc.org/fcla/dt/15240
- Subject Headings
- Freshwater algae--Florida--Everglades, Periphyton--Florida--Everglades, Stream ecology--Florida--Everglades, Primary productivity (Biology)--Florida--Everglades, Plant communities--Florida--Everglades, Everglades (Fla )
- Format
- Document (PDF)