Current Search: Flow cytometry (x)
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- Title
- Separation and concentration of phytoplankton populations using centrifugal elutriation.
- Creator
- Pomponi, Shirley A., Cucci, T. L.
- Date Issued
- 1989
- PURL
- http://purl.flvc.org/FCLA/DT/3342295
- Subject Headings
- Phytoplankton populations, Flow cytometry, Cell separation
- Format
- Document (PDF)
- Title
- Cytokinetic effects of platinum (II) polyamines on three different cell lines using flow cytometry.
- Creator
- Fitch, Claire E., Florida Atlantic University, Hartmann, James X., Louda, Deborah W.
- Abstract/Description
-
The association between cytotoxicity and cell cycle perturbation caused by cisplatin and two platinum (II) polyamines, containing a methotrexate or a 2-chloro-p-phenylenediamine component, was investigated in human ovarian carcinoma cells (CAOV3), murine Balb 3T3 fibroblasts and Chinese ovarian hamster cells by flow cytometric DNA/protein, DNA/RNA and DNA degradation assays. Continuous in vitro drug exposure caused a time- and dose-dependent suppression of growth. Flow cytometric analysis...
Show moreThe association between cytotoxicity and cell cycle perturbation caused by cisplatin and two platinum (II) polyamines, containing a methotrexate or a 2-chloro-p-phenylenediamine component, was investigated in human ovarian carcinoma cells (CAOV3), murine Balb 3T3 fibroblasts and Chinese ovarian hamster cells by flow cytometric DNA/protein, DNA/RNA and DNA degradation assays. Continuous in vitro drug exposure caused a time- and dose-dependent suppression of growth. Flow cytometric analysis revealed a multiplicity of cytokinetic perturbations, the most important of which was Go/G1 depletion and S phase arrest. The latter correlated with inhibition of DNA synthesis and subsequent inhibition of RNA and protein synthesis. Prolonged S phase arrest concomitant with DNA degradation, followed by RNA and protein degradation, resulted in cell death via apoptosis. The platinum polymers appear to be effective in vitro chemotherapeutic agents. The study of cytokinetic perturbations is useful in determining the relative potency of antitumor drugs and in monitoring drug effectiveness during treatment.
Show less - Date Issued
- 1999
- PURL
- http://purl.flvc.org/fcla/dt/15626
- Subject Headings
- Cisplatin, Cell lines, Flow cytometry, Polymers
- Format
- Document (PDF)
- Title
- Cell cycle analysis of primary sponge cell cultures.
- Creator
- Schippers, K. J., Martens, D. E.,, Pomponi, Shirley A., Wijffels, R. H.
- Date Issued
- 2011
- PURL
- http://purl.flvc.org/FCLA/DT/3318911
- Subject Headings
- Sponges--Cytology, Cell cycle, Cell proliferation, Flow cytometry
- Format
- Document (PDF)
- Title
- Development of A Portable Impedance Based Flow Cytometer for Diagnosis of Sickle Cell Disease.
- Creator
- Dieujuste, Darryl, Zhuang, Hanqi, Du, Sarah, Florida Atlantic University, College of Engineering and Computer Science, Department of Computer and Electrical Engineering and Computer Science
- Abstract/Description
-
Sickle cell disease is an inherited blood cell disorder that affects about 100,000 people in the US and results in high cost of medical care exceeding $1.1 billion annually. Sickle cell patients suffer from unpredictable, painful vaso-occlusive crises. Portable, costeffective approaches for diagnosis and monitoring sickle blood activities are important for a better management of the disease and reducing the medical cost. In this research, a mobile application controlled, impedance-based flow...
Show moreSickle cell disease is an inherited blood cell disorder that affects about 100,000 people in the US and results in high cost of medical care exceeding $1.1 billion annually. Sickle cell patients suffer from unpredictable, painful vaso-occlusive crises. Portable, costeffective approaches for diagnosis and monitoring sickle blood activities are important for a better management of the disease and reducing the medical cost. In this research, a mobile application controlled, impedance-based flow cytometer is developed for the diagnosis of sickle cell disease. Calibration of the portable device is performed using a component of known impedance value. The preliminary test results are then compared to those obtained by a commercial benchtop impedance analyzer for further validation. With the developed portable flow cytometer, experiments are performed on two sickle cell samples and a healthy cell sample. The acquired results are subsequently analyzed with MATLAB scripts to extract single-cell level impedance information as well as statistics of different cell conditions. Significant differences in cell impedance signals are observed between sickle cells and normal cells, as well as between sickle cells under hypoxia and normoxia conditions.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FA00013145
- Subject Headings
- Sickle cell disease, Sickle cell anemia--Diagnosis, Flow cytometry--Diagnostic use, Mobile Applications
- Format
- Document (PDF)