Current Search: Fatty acids--Synthesis (x)
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Title
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An investigation of the fatty acid synthase of Gymnodinium breve, a marine dinoflagellate.
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Creator
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Borrone, James William., Florida Atlantic University, Kerr, Russell G.
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Abstract/Description
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Gymnodinium breve is responsible for toxic blooms causing epizootics and, in humans, neurotoxic shellfish poisoning. Toxicity is from a family of polyketides known as brevetoxins. Proposed biosynthetic mechanisms of brevetoxin production are analogous to fatty acid biosynthesis. As part of an investigation of brevetoxin production, the fatty acid synthase from Gymnodinium breve was characterized. Cell free extracts of G. breve incorporated [3H]-acetate and [3H]-acetyl-CoA into palmitate with...
Show moreGymnodinium breve is responsible for toxic blooms causing epizootics and, in humans, neurotoxic shellfish poisoning. Toxicity is from a family of polyketides known as brevetoxins. Proposed biosynthetic mechanisms of brevetoxin production are analogous to fatty acid biosynthesis. As part of an investigation of brevetoxin production, the fatty acid synthase from Gymnodinium breve was characterized. Cell free extracts of G. breve incorporated [3H]-acetate and [3H]-acetyl-CoA into palmitate with lesser incorporation into stearate, myristate, and laurate. Fatty acid synthase activity was associated with the chloroplast, appeared to be composed of membrane-associated and soluble components, and was not completely inhibited by cerulenin. These results suggests that G. breve possesses a Type II fatty acid synthase similar in organization to those found in other photosynthetic organisms. Isolation of the enzymes, on both an enzymatic and genomic level, were hindered by low amounts G. breve cells and bacteria present within the cultures.
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Date Issued
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2000
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PURL
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http://purl.flvc.org/fcla/dt/12711
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Subject Headings
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Ptychodiscus brevis, Fatty acids--Synthesis
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Format
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Document (PDF)
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Title
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Isolation of the fatty acid synthetase from the marine invertebrate Bugula neritina.
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Creator
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Koh, Francis H., Florida Atlantic University, Kerr, Russell G., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
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Abstract/Description
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The fatty acid synthetase (FAS) from the marine invertebrate Bugula neritina was isolated using cold ethanol precipitation followed by the sequence of G-100 and G-200 size exclusion columns. Native gel analysis indicates the isolation of the FAS and the elution volume from the G-100 column suggests the FAS to be ~282kd. One band with a molecular weight of 66 kDa appeared on the SDS gel of the G-200 sample (F17-30) that eluted at 52.5 mL. The G-200 sample that eluted at 19.2 mL (F1-6)...
Show moreThe fatty acid synthetase (FAS) from the marine invertebrate Bugula neritina was isolated using cold ethanol precipitation followed by the sequence of G-100 and G-200 size exclusion columns. Native gel analysis indicates the isolation of the FAS and the elution volume from the G-100 column suggests the FAS to be ~282kd. One band with a molecular weight of 66 kDa appeared on the SDS gel of the G-200 sample (F17-30) that eluted at 52.5 mL. The G-200 sample that eluted at 19.2 mL (F1-6) displayed two predominant bands on the SDS gel corresponding to molecular weights 66 kDa and 97 kDa. Both F1-6 and F17-30 showed FAS activity displaying de novo production of myristic and palmitic acids. From the sequence of purification starting from the cell-free extract (CFE) to the F17-30 sample, a 240 fold increase in specific activity was observed. The Type II FAS experiments showed no substantial evidence of activity, namely of the beta-Hydroxybutyryl acyl-dehydrase and the enoyl reductase enzymes.
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Date Issued
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1998
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PURL
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http://purl.flvc.org/fcla/dt/15546
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Subject Headings
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Fatty acids--Synthesis, Marine pharmacology, Bryozoa
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Format
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Document (PDF)
