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Sponge-derived polyunsaturated C16 di- and tribromocarboxylic acids
Title
Sponge-derived polyunsaturated C16 di- and tribromocarboxylic acids.
Creator
Ichiba, T.,, Scheuer, P. J., Kelly-Borges, M., Harbor Branch Oceanographic Institute
Date Issued
1993
PURL
http://purl.flvc.org/FCLA/DT/3327211
Subject Headings
Sponges, Polyunsaturated fatty acids, Cytotoxin
Format
Document (PDF)
Two cytotoxic 3, 6-epidoxy fatty acids from an Indonesian sponge, Plakortis sp.
Title
Two cytotoxic 3, 6-epidoxy fatty acids from an Indonesian sponge, Plakortis sp.
Creator
Ichiba, T., Scheuer, P. J., Kelly-Borges, M., Harbor Branch Oceanographic Institute
Date Issued
1995
PURL
http://purl.flvc.org/FCLA/DT/3331902
Subject Headings
Sponges, Fatty acids, Cytotoxin, Marine natural products
Format
Document (PDF)
investigation of the fatty acid synthase of Gymnodinium breve, a marine dinoflagellate
Title
An investigation of the fatty acid synthase of Gymnodinium breve, a marine dinoflagellate.
Creator
Borrone, James William., Florida Atlantic University, Kerr, Russell G.
Abstract/Description
Gymnodinium breve is responsible for toxic blooms causing epizootics and, in humans, neurotoxic shellfish poisoning. Toxicity is from a family of polyketides known as brevetoxins. Proposed biosynthetic mechanisms of brevetoxin production are analogous to fatty acid biosynthesis. As part of an investigation of brevetoxin production, the fatty acid synthase from Gymnodinium breve was characterized. Cell free extracts of G. breve incorporated [3H]-acetate and [3H]-acetyl-CoA into palmitate with...
Show moreGymnodinium breve is responsible for toxic blooms causing epizootics and, in humans, neurotoxic shellfish poisoning. Toxicity is from a family of polyketides known as brevetoxins. Proposed biosynthetic mechanisms of brevetoxin production are analogous to fatty acid biosynthesis. As part of an investigation of brevetoxin production, the fatty acid synthase from Gymnodinium breve was characterized. Cell free extracts of G. breve incorporated [3H]-acetate and [3H]-acetyl-CoA into palmitate with lesser incorporation into stearate, myristate, and laurate. Fatty acid synthase activity was associated with the chloroplast, appeared to be composed of membrane-associated and soluble components, and was not completely inhibited by cerulenin. These results suggests that G. breve possesses a Type II fatty acid synthase similar in organization to those found in other photosynthetic organisms. Isolation of the enzymes, on both an enzymatic and genomic level, were hindered by low amounts G. breve cells and bacteria present within the cultures.
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Date Issued
2000
PURL
http://purl.flvc.org/fcla/dt/12711
Subject Headings
Ptychodiscus brevis, Fatty acids--Synthesis
Format
Document (PDF)
Isolation of the fatty acid synthetase from the marine invertebrate Bugula neritina
Title
Isolation of the fatty acid synthetase from the marine invertebrate Bugula neritina.
Creator
Koh, Francis H., Florida Atlantic University, Kerr, Russell G., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
Abstract/Description
The fatty acid synthetase (FAS) from the marine invertebrate Bugula neritina was isolated using cold ethanol precipitation followed by the sequence of G-100 and G-200 size exclusion columns. Native gel analysis indicates the isolation of the FAS and the elution volume from the G-100 column suggests the FAS to be ~282kd. One band with a molecular weight of 66 kDa appeared on the SDS gel of the G-200 sample (F17-30) that eluted at 52.5 mL. The G-200 sample that eluted at 19.2 mL (F1-6)...
Show moreThe fatty acid synthetase (FAS) from the marine invertebrate Bugula neritina was isolated using cold ethanol precipitation followed by the sequence of G-100 and G-200 size exclusion columns. Native gel analysis indicates the isolation of the FAS and the elution volume from the G-100 column suggests the FAS to be ~282kd. One band with a molecular weight of 66 kDa appeared on the SDS gel of the G-200 sample (F17-30) that eluted at 52.5 mL. The G-200 sample that eluted at 19.2 mL (F1-6) displayed two predominant bands on the SDS gel corresponding to molecular weights 66 kDa and 97 kDa. Both F1-6 and F17-30 showed FAS activity displaying de novo production of myristic and palmitic acids. From the sequence of purification starting from the cell-free extract (CFE) to the F17-30 sample, a 240 fold increase in specific activity was observed. The Type II FAS experiments showed no substantial evidence of activity, namely of the beta-Hydroxybutyryl acyl-dehydrase and the enoyl reductase enzymes.
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Date Issued
1998
PURL
http://purl.flvc.org/fcla/dt/15546
Subject Headings
Fatty acids--Synthesis, Marine pharmacology, Bryozoa
Format
Document (PDF)
Fatty acid trophic markers and trophic links among seston, crustaceanzooplankton and the siphonophore Nanomia cara in Georges Basin and Oceanographer Canyon (NWAtlantic)
Title
Fatty acid trophic markers and trophic links among seston, crustaceanzooplankton and the siphonophore Nanomia cara in Georges Basin and Oceanographer Canyon (NWAtlantic).
Creator
Rossi, S., Youngbluth, Marsh J., Jacoby, C. A., Pages, F., Garrofe, X.
Date Issued
2008
PURL
http://purl.flvc.org/fau/fd/FA00007246
Subject Headings
Maine, Gulf of, Siphonophores, Fatty acids, Food chains (Ecology)
Format
Document (PDF)
Essential fatty acid requirement of the Chinese prawn, Penaeus chinensis
Title
Essential fatty acid requirement of the Chinese prawn, Penaeus chinensis.
Creator
Xu, X. L., Ji, W. J., Castell, J. D., O'Dor, R. K., Harbor Branch Oceanographic Institute
Date Issued
1994
PURL
http://purl.flvc.org/FCLA/DT/3331878
Subject Headings
Penaeus chinensis, Essential fatty acids, Crustacea--Feeding and feeds
Format
Document (PDF)