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STUDIES ON POLYETHYLENE GLYCOL-MEDIATED AGGREGATION AND FUSION OF AVIAN ERYTHROCYTES
Title
STUDIES ON POLYETHYLENE GLYCOL-MEDIATED AGGREGATION AND FUSION OF AVIAN ERYTHROCYTES.
Creator
BATCHELOR, ROGER ALLEN., Florida Atlantic University
Abstract/Description
Polyethylene glycol (PEG)-induced aggregation, hemolysis and fusion of chicken red blood ceils (CRBC's) was studied in relation to the following parameters: osmotic pressure, polymer concentration, ionic strength, electrolyte species, surface charge removal and glutaraldehyde fixation. Indirect evidence indicated that osmotic pressure does not play a primary role in aggregation and fusion of red blood cells. Maximum aggregation and fusion was obtained in solutions of 20 and 40% PEG,...
Show morePolyethylene glycol (PEG)-induced aggregation, hemolysis and fusion of chicken red blood ceils (CRBC's) was studied in relation to the following parameters: osmotic pressure, polymer concentration, ionic strength, electrolyte species, surface charge removal and glutaraldehyde fixation. Indirect evidence indicated that osmotic pressure does not play a primary role in aggregation and fusion of red blood cells. Maximum aggregation and fusion was obtained in solutions of 20 and 40% PEG, respectively. In contrast, hemolysis increased almost linearily with PEG concentration. The negative electrostatic charge due to the presence of sialic acid on the membrane surface was found to be the primary factor affecting aggregation, hemolysis and fusion. Removal of the charge by neuraminidase or screening with cations enhanced aggregation and fusion while inhibiting hemolysis. The inhibition of aggregation by glutaraldehyde fixation, and hemolysis and fusion results not attributed to surface charges, are discussed in terms of intramembrane interactions with PEG.
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Date Issued
1977
PURL
http://purl.flvc.org/fcla/dt/13893
Subject Headings
Cell hybridization, Erythrocytes, Hemolysis and hemolysins, Membrane fusion
Format
Document (PDF)
SUPPORTED ERYTHROCYTE MEMBRANES ON PIEZOELECTRIC SENSORS FOR STUDYING THE INTERACTIONS BETWEEN NANOPARTICLES AND SURFACES OF RED BLOOD CELLS
Title
SUPPORTED ERYTHROCYTE MEMBRANES ON PIEZOELECTRIC SENSORS FOR STUDYING THE INTERACTIONS BETWEEN NANOPARTICLES AND SURFACES OF RED BLOOD CELLS.
Creator
Islam, Tanaz, Yi, Peng, Florida Atlantic University, Department of Civil, Environmental and Geomatics Engineering, College of Engineering and Computer Science
Abstract/Description
The Supported red blood cell membrane (SRBCm) was developed on a piezoelectric sensor to study the attachment of nanoparticles to erythrocyte surfaces. A well-dispersed colloidal suspension of fragments of RBCm was prepared from whole blood, and characterized thoroughly using cryogenic transmission electron microscopy, dynamic light scattering, and zeta potential analysis. To develop SRBCm, RBCm fragments were immobilized onthe sensor in a quartz crystal microbalance with dissipation...
Show moreThe Supported red blood cell membrane (SRBCm) was developed on a piezoelectric sensor to study the attachment of nanoparticles to erythrocyte surfaces. A well-dispersed colloidal suspension of fragments of RBCm was prepared from whole blood, and characterized thoroughly using cryogenic transmission electron microscopy, dynamic light scattering, and zeta potential analysis. To develop SRBCm, RBCm fragments were immobilized onthe sensor in a quartz crystal microbalance with dissipation monitoring system. A complete monolayer of flattened fragments of RBCm was formed on the positively charged surface of the piezoelectric sensor in 1 mM NaCl and 0.2 mM NaHCO3 at pH 7.1. The surface morphology of SRBCm was characterized via atomic force microscopy. The even distribution of surface proteins expressed on erythrocytes was found on SRBCm through indirect immunofluorescence microscopy. The attachment efficiencies of model nanoparticles, e.g. hematite nanoparticles and carboxylated polystyrene nanoparticles, on the SRBCm were quantified using a classic methodology. KEYWORDS: Supported erythrocyte membrane, piezoelectric sensor, phospholipid bilayers, nanoparticles
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Date Issued
2019
PURL
http://purl.flvc.org/fau/fd/FA00013383
Subject Headings
Erythrocyte Membrane, Piezoelectric polymer biosensors, Nanoparticles, Phospholipid bilayers, Bilayer lipid membranes
Format
Document (PDF)