Current Search: Enzymes--Synthesis (x)
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- Title
- Development of methods for the immobilization of enzymes used in 9,11-secosteroid biosynthesis.
- Creator
- Chen, Ying, Florida Atlantic University, Kerr, Russell G.
- Abstract/Description
-
9,11-Secosteroids exhibit novel biological activity and interesting pharmacological properties. A biosynthetic study of 9,11-secogorgosterol and other novel 9,11-secosteroids using an enzyme extract from the gorgonian Pseudopterogorgia americana demonstrated that this system is an efficient catalyst for a wide range 9,11-secosteroid production. Two methods to recover the enzyme from 9,11-secosteroid synthesis have been developed: (1) membrane-enclosed enzymatic catalysis, which combines...
Show more9,11-Secosteroids exhibit novel biological activity and interesting pharmacological properties. A biosynthetic study of 9,11-secogorgosterol and other novel 9,11-secosteroids using an enzyme extract from the gorgonian Pseudopterogorgia americana demonstrated that this system is an efficient catalyst for a wide range 9,11-secosteroid production. Two methods to recover the enzyme from 9,11-secosteroid synthesis have been developed: (1) membrane-enclosed enzymatic catalysis, which combines certain of the advantages of soluble enzymes and immobilized enzymes, shows high activity, simple recovery and at least 5 cycles or 23 days; (2) covalently bonded enzyme-agarose gel, attached by cyanogen bromide via the amino groups of enzymes, show 80% activity of free enzymes and at least 7 cycles or 46 days. Based on stability and kinetic studies of these immobilized enzymes in 9,11-secosteroid synthesis, a continuous flow column reactor for scale-up of 9,11-secosteroid production has been developed with great efficiency, which represents the first enzymatic production of a bioactive compound from a marine invertebrate on a large scale.
Show less - Date Issued
- 1998
- PURL
- http://purl.flvc.org/fcla/dt/15521
- Subject Headings
- Octocorallia, Enzymes--Synthesis, Steroids
- Format
- Document (PDF)
- Title
- Biosynthesis and enzymology of secosteroids from Pseudopterogorgia americana.
- Creator
- Head, Kevin, Florida Atlantic University, Kerr, Russell G.
- Abstract/Description
-
9,11-Secosteroids are biologically active secondary metabolites from the marine invertebrate, Pseudopterogorgia americana. They are produced in vitro through an incubation of the steroid substrate with a cell-free extract of P. americana containing the necessary enzymes. Further optimization of this incubation was attempted through adjustment of incubation reagents and cofactors. The enzymes responsible for secosteroid production were partially purified through chromatography in an effort to...
Show more9,11-Secosteroids are biologically active secondary metabolites from the marine invertebrate, Pseudopterogorgia americana. They are produced in vitro through an incubation of the steroid substrate with a cell-free extract of P. americana containing the necessary enzymes. Further optimization of this incubation was attempted through adjustment of incubation reagents and cofactors. The enzymes responsible for secosteroid production were partially purified through chromatography in an effort to isolate and purify these enzymes. Finally, experiments with radiolabeled gorgosterol led to the elucidation and isolation of a key secosteroid precursor, 9,11-dihydroxygorgosterol.
Show less - Date Issued
- 1999
- PURL
- http://purl.flvc.org/fcla/dt/15711
- Subject Headings
- Alcyonacea, Enzymes--Synthesis, Sterols
- Format
- Document (PDF)
- Title
- Chemoenzymatic synthesis of 9,11-secosteroids using an enzyme extract from a marine coral.
- Creator
- Rodriguez, Lesbeth Caridad., Florida Atlantic University, Kerr, Russell G.
- Abstract/Description
-
9,11-Secogorgosterol, a secondary metabolite from the gorgonian Pseudopterogorgia americana, exhibits inhibitory activity against protein kinase C, and potent anti-proliferative and anti-inflammatory activity. An efficient method for the production of 9,11-secogorgosterol has been developed and optimized using an enzyme extract from the gorgonian P. americana. The gorgonian also produces two other 9,11-secosteroids which have marked differences in their side chains and nuclei, which suggested...
Show more9,11-Secogorgosterol, a secondary metabolite from the gorgonian Pseudopterogorgia americana, exhibits inhibitory activity against protein kinase C, and potent anti-proliferative and anti-inflammatory activity. An efficient method for the production of 9,11-secogorgosterol has been developed and optimized using an enzyme extract from the gorgonian P. americana. The gorgonian also produces two other 9,11-secosteroids which have marked differences in their side chains and nuclei, which suggested that the enzymes responsible for their production were likely relatively nonspecific. Novel 9,11-secosteroids have been synthesized using the enzyme extract from the gorgonian.
Show less - Date Issued
- 1997
- PURL
- http://purl.flvc.org/fcla/dt/15431
- Subject Headings
- Steroids, Enzymes--Synthesis, Alcyonacea, Corals
- Format
- Document (PDF)