Current Search: Developmental biology (x)
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(1 - 4 of 4)
- Title
- Investigation of talin head-tail interactions.
- Creator
- Butyn, Amber., Harriet L. Wilkes Honors College
- Abstract/Description
-
Talin is a ubiquitous, high-molecular-weight, flexible protein that plays a critical role in focal adhesions by activating, as well as connecting, integrins to the actin cytoskeleton. Talin's inactive auto-inhibitory state is speculated to be one of its modes of regulation inside the cell and is achieved through its head-tail interactions. In order to decipher the stability of this interaction, the head domain (residues 206-405) was cloned into a modified pET28m vector while the tail domains ...
Show moreTalin is a ubiquitous, high-molecular-weight, flexible protein that plays a critical role in focal adhesions by activating, as well as connecting, integrins to the actin cytoskeleton. Talin's inactive auto-inhibitory state is speculated to be one of its modes of regulation inside the cell and is achieved through its head-tail interactions. In order to decipher the stability of this interaction, the head domain (residues 206-405) was cloned into a modified pET28m vector while the tail domains (residues 1654-2344 and 2225-2344) were cloned into the pET32a vector to obtain octa-histidine tagged and un-tagged peptide, respectively. Neither co-expression nor pull-down using the His-tagged head domain was successful in purifying a stable head-tail complex. Our results indicate rather weak interactions between the talin head and rod domains and hence, under our experimental conditions, do not lead to a stable auto-inhibitory complex that can be purified for further studies.
Show less - Date Issued
- 2009
- PURL
- http://purl.flvc.org/FAU/209984
- Subject Headings
- Membrane proteins, Structure-activity relationships, Cell membranes, Physiology, Molecular biology, Cell interaction, Developmental cytology
- Format
- Document (PDF)
- Title
- Ecosystem health and environmental influences on innate immune function in the loggerhead (Caretta caretta) and green (Chelonia mydas) sea turtle.
- Creator
- Sposato, Patricia L., Milton, Sarah L., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Loggerhead (Caretta caretta) and green (Chelonia mydas) turtles recruit to nearshore environments as juveniles. These often degraded habitats are associated with emerging diseases such as green turtle fibropapillomatosis (GTFP), however there are few studies on immune function in sea turtles. The objective of this research was to quantify phagocytosis of the innate immune system by flow cytometry and compare levels between animals from a degraded habitat (the Indian River Lagoon, FL) to a...
Show moreLoggerhead (Caretta caretta) and green (Chelonia mydas) turtles recruit to nearshore environments as juveniles. These often degraded habitats are associated with emerging diseases such as green turtle fibropapillomatosis (GTFP), however there are few studies on immune function in sea turtles. The objective of this research was to quantify phagocytosis of the innate immune system by flow cytometry and compare levels between animals from a degraded habitat (the Indian River Lagoon, FL) to a more pristine environment (the Trident Basin, Port Canaveral, FL), and across a range of temperatures. While in vitro temperatures did not alter rates of phagocytosis, it was higher in samples obtained in the summer than winter. Rates of phagocytosis in sea turtles with GTFP and from degraded environments with increased prevalence of GTFP were low compared to animals from the more pristine environment, suggesting that the environment can alter innate immunological function and thus contribute to the development of disease.
Show less - Date Issued
- 2014
- PURL
- http://purl.flvc.org/fau/fd/FA00004333, http://purl.flvc.org/fau/fd/FA00004333
- Subject Headings
- Developmental biology, Ecosystem management -- Florida, Loggerhead turtle -- Habitat -- Environmental aspects, Marine biodiversity conservation, Sea turtles -- Immunology
- Format
- Document (PDF)
- Title
- Genetic differentiation among populations of bald eagles, Haliaeetus leucocephalus.
- Creator
- Helmick, Ericka Elizabeth., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
The bald eagle, Haliaeetus leucocephalus, population declined dramatically in the early 20th century reducing the population from tens of thousands of birds within the lower 48 states, to
Show moreThe bald eagle, Haliaeetus leucocephalus, population declined dramatically in the early 20th century reducing the population from tens of thousands of birds within the lower 48 states, to <450 pairs of birds, effectively inducing a population bottleneck. The overall population has recovered and was removed from the endangered species list in 2007. This study investigates whether such overall population statistics are appropriate descriptors for this widespread species. I investigated the genetic differentiation between three populations of bald eagles from Alaska, North Florida and Florida Bay using both mitochondrial and nuclear DNA loci to determine whether discrete subpopulations comprise the broad range. Significant FST values, for both mtDNA and microsatellites, were found between both Florida populations and Alaska, but not within Florida populations. Results indicate that there is strong population structure, rejecting the null hypothesis of a panmictic population. Future conservation efforts should focus on subpopulations rather than the overall population.
Show less - Date Issued
- 2011
- PURL
- http://purl.flvc.org/FAU/3171395
- Subject Headings
- Wildlife conservation, Birds, Molecular genetics, Gene targeting, Developmental biology, Biochemical markers
- Format
- Document (PDF)
- Title
- A comparison of the ontogeny of energy consumption in leatherback, Dermochelys coriacea and olive ridley, Lepidochelys olivacea sea turtle hatchlings.
- Creator
- Jones, T. Todd, Florida Atlantic University, Lutz, Peter L., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Changes in activity related oxygen consumption and energy partitioning were measured in leatherback and olive ridley sea turtle hatchlings over their first month after nest emergence. Leatherbacks emerge with about 75--90 KJ of energy in the residual yolk at their disposal for growth and movement. In comparison, the residual yolk energy reserves for the olive ridley are estimated to be much less (45 KJ). In leatherbacks resting specific oxygen consumption rates decreased by 53% over the first...
Show moreChanges in activity related oxygen consumption and energy partitioning were measured in leatherback and olive ridley sea turtle hatchlings over their first month after nest emergence. Leatherbacks emerge with about 75--90 KJ of energy in the residual yolk at their disposal for growth and movement. In comparison, the residual yolk energy reserves for the olive ridley are estimated to be much less (45 KJ). In leatherbacks resting specific oxygen consumption rates decreased by 53% over the first post-hatching month (0.0065 ml O2 min-1 g-1--0.0031 ml O2 min-1 g-1), while for ridleys the fall was 32% (0.0038 ml O2 min-1 g-1--0.0026 ml O2 min-1 g-1). Greater differences were seen in aerobic scope. For olive ridleys the factorial aerobic scope doubled over the first month but there was no significant increase in the leatherback's factorial aerobic scope. Leatherback hatchlings gained on average 33% body mass (10 g) over the first week however 70 to 80% of this increase was due to water accumulation. The differences in aerobic scope and energy reserves are related to differences in early life ecological stratagems of these species.
Show less - Date Issued
- 2004
- PURL
- http://purl.flvc.org/fcla/dt/13139
- Subject Headings
- Sea turtles--Ecology, Animal behavior, Ontogeny, Developmental biology, Adaptation (Physiology), Ecophysiology
- Format
- Document (PDF)
