Current Search: Cisplatin (x)
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Title
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Cytokinetic effects of platinum (II) polyamines on three different cell lines using flow cytometry.
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Creator
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Fitch, Claire E., Florida Atlantic University, Hartmann, James X., Louda, Deborah W.
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Abstract/Description
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The association between cytotoxicity and cell cycle perturbation caused by cisplatin and two platinum (II) polyamines, containing a methotrexate or a 2-chloro-p-phenylenediamine component, was investigated in human ovarian carcinoma cells (CAOV3), murine Balb 3T3 fibroblasts and Chinese ovarian hamster cells by flow cytometric DNA/protein, DNA/RNA and DNA degradation assays. Continuous in vitro drug exposure caused a time- and dose-dependent suppression of growth. Flow cytometric analysis...
Show moreThe association between cytotoxicity and cell cycle perturbation caused by cisplatin and two platinum (II) polyamines, containing a methotrexate or a 2-chloro-p-phenylenediamine component, was investigated in human ovarian carcinoma cells (CAOV3), murine Balb 3T3 fibroblasts and Chinese ovarian hamster cells by flow cytometric DNA/protein, DNA/RNA and DNA degradation assays. Continuous in vitro drug exposure caused a time- and dose-dependent suppression of growth. Flow cytometric analysis revealed a multiplicity of cytokinetic perturbations, the most important of which was Go/G1 depletion and S phase arrest. The latter correlated with inhibition of DNA synthesis and subsequent inhibition of RNA and protein synthesis. Prolonged S phase arrest concomitant with DNA degradation, followed by RNA and protein degradation, resulted in cell death via apoptosis. The platinum polymers appear to be effective in vitro chemotherapeutic agents. The study of cytokinetic perturbations is useful in determining the relative potency of antitumor drugs and in monitoring drug effectiveness during treatment.
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Date Issued
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1999
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PURL
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http://purl.flvc.org/fcla/dt/15626
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Subject Headings
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Cisplatin, Cell lines, Flow cytometry, Polymers
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Format
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Document (PDF)
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Title
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Inhibition of DNA synthesis by platinum (II) polyamines.
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Creator
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Colvin, Alex Charles., Florida Atlantic University, Siegmann, Deborah
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Abstract/Description
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Cis-DDP is a chemotherapeutic drug which has been very useful in the treatment of testicular and ovarian cancers. Recently, polymeric drugs modeled after cis-DDP have been developed by reacting potassium tetrachloroplatinate with various diamines. Previous testing of these analogs on cells indicated that many were cytotoxic. To see if the cytotoxic effects were the result of inhibition of DNA synthesis as is thought to be the case for cis-DDP, 3H-thymidine incorporation studies were carried...
Show moreCis-DDP is a chemotherapeutic drug which has been very useful in the treatment of testicular and ovarian cancers. Recently, polymeric drugs modeled after cis-DDP have been developed by reacting potassium tetrachloroplatinate with various diamines. Previous testing of these analogs on cells indicated that many were cytotoxic. To see if the cytotoxic effects were the result of inhibition of DNA synthesis as is thought to be the case for cis-DDP, 3H-thymidine incorporation studies were carried out on polymer-treated cells. The results were compared to incorporation studies from cis-DDP-treated cells and found to be similar. The results from the DNA synthesis studies were compared to cytotoxic results, and they indicated that inhibition of DNA synthesis may be responsible for the cytotoxic effects of the polymers. The size of a polymer was found to be unimportant in determining its effectiveness.
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Date Issued
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1989
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PURL
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http://purl.flvc.org/fcla/dt/14547
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Subject Headings
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DNA--Synthesis, Cisplatin--Physiological effect, Platinum compounds--Physiological effect
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Format
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Document (PDF)
