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Title: The establishment and study of two elasmobranch cell lines.
Creator: Poyer, James Christopher., Florida Atlantic University, Hartmann, James X.
Abstract/Description: The present study describes the first cell lines produced from members of class Chondrichthyes. Explants of brain tissue from Carcharhinus falciformis (silky shark) and Ginglymostoma cirratum (nurse shark) were incubated in a mammalian medium modified with the addition of urea, trimethylamine N-oxide, NaCl, and bovine serum. Primary monolayers were passaged with 0.025% trypsin in a modified saline solution. Silky shark cells grew optimally at 29C. The population doubling time for C....
Show moreThe present study describes the first cell lines produced from members of class Chondrichthyes. Explants of brain tissue from Carcharhinus falciformis (silky shark) and Ginglymostoma cirratum (nurse shark) were incubated in a mammalian medium modified with the addition of urea, trimethylamine N-oxide, NaCl, and bovine serum. Primary monolayers were passaged with 0.025% trypsin in a modified saline solution. Silky shark cells grew optimally at 29C. The population doubling time for C. falciformis cells at passage 29 was 67 hours. For G. cirratum cells at passage 6 the population doubling was 84 hours. Silky shark cells grew over a broad range of osmolalities from 315 mOsm to a 1664 mOsm with optimal growth at 650 mOsm. A medium containing 10% dimethylsulfoxide allowed for cryopreservation with greater than 65% viability upon recovery. Current theories of elasmobranch osmoregulation are discussed in light of experimental data collected from studies conducted on the silky shark cell line.
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Date Issued: 1992
PURL: http://purl.flvc.org/fcla/dt/14795
Subject Headings: Sharks, Cell culture, Chondrichthyes
Format: Document (PDF)

Title: Biology of the Porifera: cell culture.
Creator: Pomponi, Shirley A.
Date Issued: 2006
PURL: http://purl.flvc.org/FCLA/DT/3342232
Subject Headings: Porifera, Sponges, Cell culture, Biology
Format: Document (PDF)

Title: EVALUATION OF 3-D CULTURE METHODS FOR MARINE SPONGES.
Creator: Urban-Gedamke, Elizabeth, Pomponi, Shirley A., Florida Atlantic University, Department of Marine Science and Oceanography, Charles E. Schmidt College of Science
Abstract/Description: Marine sponges are economically and environmentally valuable, but restoration, commercial, and biomedical demands exceed what wild populations and aquaculture can provide. In vitro culture of sponge cells is a promising alternative, but has remained elusive until recent breakthroughs involving improved nutrient medium M1 in two-dimensional culture. The advantages of three-dimensional over two-dimensional cell culture have been increasingly recognized. Here we report the successful 21-day...
Show moreMarine sponges are economically and environmentally valuable, but restoration, commercial, and biomedical demands exceed what wild populations and aquaculture can provide. In vitro culture of sponge cells is a promising alternative, but has remained elusive until recent breakthroughs involving improved nutrient medium M1 in two-dimensional culture. The advantages of three-dimensional over two-dimensional cell culture have been increasingly recognized. Here we report the successful 21-day culture of cells from the marine sponge Geodia neptuni using multiple three-dimensional cell culture methods: FibraCel© disks, thin hydrogel layers, gel micro droplets, and spheroid cell culture. These methods performed comparably to two-dimensional control cultures, and each method offers advantages for restoration or in vitro applications using sponge cells. Further optimization of these methods may lead to the ability to culture fully functioning sponges from dissociated, cryopreserved cells, which will reduce the need for wild harvest of sponge tissues for commercial, restoration, and biomedical purposes.
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Date Issued: 2021
PURL: http://purl.flvc.org/fau/fd/FA00013726
Subject Headings: Sponges, Cell Culture--methods
Format: Document (PDF)

Title: Quantitative assessment of marine sponge cells in vitro: Development of improved growth medium.
Creator: Willoughby, Robin, Pomponi, Shirley A.
Date Issued: 2000
PURL: http://purl.flvc.org/FCLA/DT/2795606
Subject Headings: Animal cell biotechnology, Marine biology, Cell culture --Technique
Format: Document (PDF)

Title: Sponge cell culture.
Creator: Pomponi, Shirley A., Willoughby, Robin, Kelly-Borges, M.
Date Issued: 1998
PURL: http://purl.flvc.org/fau/fd/FA00007453
Subject Headings: Sponges, Sponges--Cytology, Cell culture
Format: Document (PDF)

Title: Sponge cell culture for production of bioactive metabolites.
Creator: Pomponi, Shirley A., Willoughby, Robin
Date Issued: 1994
PURL: http://purl.flvc.org/fau/fd/FA00007381
Subject Headings: Sponges, Cell culture, Metabolites, Bioactive compounds
Format: Document (PDF)

Title: Molecular Characterization of a Nicotiana tabacum Chloroplast Mutant and in vitro Regeneration of a Cattail, Typha domingensis, from Embryonic Callus.
Creator: Tapia, Claudius E., Zhang, Xing-Hai, Florida Atlantic University
Abstract/Description: The thesis consists of two chapters, and within each chapter is a different set of methods and techniques that will be useful to me in future research endeavors. The first is the transformation of tobacco chloroplast and the analysis of a resultant chloroplast mutant, and the second is the establishment of a regeneration system for the aquatic plant Typha domingensis, commonly known as cattail. The unifying theme is plant transformation. The establishment of a regeneration system for a...
Show moreThe thesis consists of two chapters, and within each chapter is a different set of methods and techniques that will be useful to me in future research endeavors. The first is the transformation of tobacco chloroplast and the analysis of a resultant chloroplast mutant, and the second is the establishment of a regeneration system for the aquatic plant Typha domingensis, commonly known as cattail. The unifying theme is plant transformation. The establishment of a regeneration system for a potentially beneficial plant is useful for future transformations and the actual transformation and analysis of mutants is useful for the characterization of transformants. The chloroplast transformation was unsuccessful and analysis of the mutation demonstrated it as a null mutation under normal growth conditions. Cattail seeds were induced to form calli, then induced to regenerate into normal plants.
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Date Issued: 2007
PURL: http://purl.flvc.org/fau/fd/FA00000839
Subject Headings: Plant cell culture, Plant biotechnology, Typha
Format: Document (PDF)

Title: CELL CULTURE RESPONSES TO EQUINE INFECTIOUS ANEMIA VIRUS.
Creator: MANKAMYER, MARY PAT, Florida Atlantic University
Abstract/Description: Liver, kidney and spleen cells from healthy horses and horses with equine infectious anemia were cultured in vitro. Primary culture of organs from healthy horses yielded typical elongate fibroblast cells. Primary culture of organs from diseased horses yielded a variety of cell types; pleomorphic giant cells being most common. The giant cells did not divide, but either underwent degeneration and death or became transformed into squamous-like cells lacking contact inhibition resulting in the...
Show moreLiver, kidney and spleen cells from healthy horses and horses with equine infectious anemia were cultured in vitro. Primary culture of organs from healthy horses yielded typical elongate fibroblast cells. Primary culture of organs from diseased horses yielded a variety of cell types; pleomorphic giant cells being most common. The giant cells did not divide, but either underwent degeneration and death or became transformed into squamous-like cells lacking contact inhibition resulting in the production of foci. Inoculation of normal equine fibroblasts with either serum, plasma, or liver, kidney or spleen extracts from diseased horses resulted in a proliferation of the fibroblasts. Normal liver or kidney fibroblasts cocultivated with equine leucocytes and inoculated with serum or plasma from a diseased horse became transformed. This research supports the proposal that equine infectious anemia virus is an oncornavirus.
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Date Issued: 1974
PURL: http://purl.flvc.org/fcla/dt/13651
Subject Headings: Equine infectious anemia, Horses--Diseases, Cell culture
Format: Document (PDF)

Title: Understanding the role of transgenic catalase in T-cell development in murine-based studies.
Creator: Smith, Richard M., Harriet L. Wilkes Honors College
Abstract/Description: The thymus provides a unique microenvironment that facilitates T lymphocytes differentiation and maturation. However, the thymus atrophies after puberty which leads to an overall expression of metabolism gene pathways and low gene expression of certain peroxide scavenger enzymes such as catalase in thymic stromal compartments. From this data, we postulate that thymic stromal cells are highly susceptible to oxidative damage. We utilized a transgenic mice model overexpressing human catalase...
Show moreThe thymus provides a unique microenvironment that facilitates T lymphocytes differentiation and maturation. However, the thymus atrophies after puberty which leads to an overall expression of metabolism gene pathways and low gene expression of certain peroxide scavenger enzymes such as catalase in thymic stromal compartments. From this data, we postulate that thymic stromal cells are highly susceptible to oxidative damage. We utilized a transgenic mice model overexpressing human catalase targeted to the mitochondria (mCat) to test our hypothesis that gerater oxidative protection should lower the degree of thymus atrophy. Our experiment focused on a direct comparison of organ weights (thymus, kidney, lymph nodes, spleen and heart), cellularity and histology between transgenic and wildtype mice. We found that mCat had selective increases in thymus size.
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Date Issued: 2012
PURL: http://purl.flvc.org/FAU/3359325
Subject Headings: T cells, Differentiation, Developmental genetics, Gene expression, Human cell culture, Thymus, Physiology
Format: Document (PDF)

Title: Cellular Basis of Sponge-Sponge Associations.
Creator: Conkling, Megan, Pomponi, Shirley A., Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Marine sponges interact and coexist with many different organisms. A two-sponge association between Amphimedon erina and Geodia gibberosa commonly occurs in the Florida Keys. Previous studies have only focused on the ecological influence of the association; they did not examine the cellular basis of the association. This association between A. erina and G. gibberosa was used in the development of an in vitro model to further the understanding of the cellular basis of natural sponge-sponge...
Show moreMarine sponges interact and coexist with many different organisms. A two-sponge association between Amphimedon erina and Geodia gibberosa commonly occurs in the Florida Keys. Previous studies have only focused on the ecological influence of the association; they did not examine the cellular basis of the association. This association between A. erina and G. gibberosa was used in the development of an in vitro model to further the understanding of the cellular basis of natural sponge-sponge associations. In this study, sponge cells were cultured individually and in co-cultures and their responses related to apoptosis, cell death, and proliferation were monitored using high content imaging. Co-cultured cells of species that form sponge-sponge associations did not have the same cellular responses compared to co-cultured cells of species that do not form sponge-sponge associations. Protein expression analyses demonstrated that the model that was established does not mimic the cellular response of the association in nature, but this model can be used to test in vitro cellular interactions of sponge species that do not form associations in nature. In addition, the protein expression data that were obtained revealed that sponges use similar apoptotic pathways as humans and suggest that sponge cells may shut down cell cycling in order to repair damaged DNA. This research is a small piece to the puzzle that is sponge cell culture research.
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Date Issued: 2017
PURL: http://purl.flvc.org/fau/fd/FA00004848, http://purl.flvc.org/fau/fd/FA00004848
Subject Headings: Sponges--Habitat--Florida., Marine invertebrates--Florida., Aquatic invertebrates--Cultures and culture media., Apoptosis., Cell culture., Symbiosis.
Format: Document (PDF)

Title: Spatial analysis and functional gene clustering between lens epithelium and fiber cells.
Creator: Cowell, Tracy L., Florida Atlantic University, Kantorow, Marc
Abstract/Description: Purpose. To identify genes important for maintaining the specialized functions of lens epithelial and fiber cells. Methods. The expression profiles of 22,215 genes between human lens epithelial and fiber cells were analyzed using oligonucleotide microarray hybridization and RT-PCR. Selected genes were functionally clustered using the EASE bioinformatics software package. Results. Analysis of microarray hybridizations revealed 1430 transcripts that were significantly increased and 901...
Show morePurpose. To identify genes important for maintaining the specialized functions of lens epithelial and fiber cells. Methods. The expression profiles of 22,215 genes between human lens epithelial and fiber cells were analyzed using oligonucleotide microarray hybridization and RT-PCR. Selected genes were functionally clustered using the EASE bioinformatics software package. Results. Analysis of microarray hybridizations revealed 1430 transcripts that were significantly increased and 901 transcripts that were significantly decreased. Microarray data was confirmed using RT-PCR on 11 randomly selected genes. Functional clustering of the identified gene expression patterns revealed altered gene expression in cellular pathways including oxidative stress, cell proliferation, and apoptosis. The methionine sulfoxide reductase class of enzymes were further analyzed and demonstrated to be expressed throughout the human body, indicating a significant protective role. Conclusions. These data reveal novel and previously identified gene expression differences that provide insight into those mechanisms that may be important for lens cell differentiation.
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Date Issued: 2004
PURL: http://purl.flvc.org/fcla/dt/13193
Subject Headings: Gene mapping--Statistical methods, Eye--Physiology, Epithelium--Culture and culture media, Cell culture--Analysis
Format: Document (PDF)

Title: Development of techniques for in vitro production of bioactive natural products from marine sponges.
Creator: Pomponi, Shirley A., Willoughby, Robin, Kaighn, M. E., Wright, Amy E.
Date Issued: 1997
PURL: http://purl.flvc.org/fau/fd/FA00007344
Subject Headings: Sponges, Bioactive compounds, Marine natural products, Cell culture, Growth factors, Phytohemagglutinins, Cryopreservation
Format: Document (PDF)

Title: Reciprocal regulation between taurine and glutamate response via Ca2+ - dependent pathways in retinal third-order neurons.
Creator: Bulley, Simon, Shen, Wen
Date Issued: 2010-08-24
PURL: http://purl.flvc.org/fcla/dt/3327274
Subject Headings: Amacrine Cells*/cytology, Amacrine Cells*/drug effects, Amacrine Cells*/metabolism, Ambystoma, Calcium/metabolism, Calcium Channels/metabolism, Cells, Cultured, Enzyme Inhibitors/metabolism, Excitatory Amino Acid Agonists/pharmacology, GABA Antagonists/pharmacology, Glutamic Acid/metabolism, Glycine Agents/pharmacology, Kainic Acid/pharmacology, Membrane Glycoproteins, Membrane Potentials, Neurotransmitter Agents, Retinal Ganglion Cells, Signal Transduction, Synaptic Transmission
Format: Document (PDF)

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