Current Search: Calcium-binding proteins (x)
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Title
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Characterization and distribution of parvalbumin from selected fish species.
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Creator
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Ross, Cliff Ian., Florida Atlantic University, Hartmann, James X., Mari, Frank
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Abstract/Description
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Parvalbumins are acidic calcium-binding proteins found in large quantities in the white muscle fibers of cold-blooded vertebrates. Fish display two to seven parvalbumin isotypes that are species specific and thus constitute a valuable tool in the study of phylogenetic relationships, and as a specific biomarker for fish identification. Parvalbumins were isolated from a selected sample of marine teleosts and elasmobranchs. Purified isotypes were characterized via HPLC (gel filtration, and ion...
Show moreParvalbumins are acidic calcium-binding proteins found in large quantities in the white muscle fibers of cold-blooded vertebrates. Fish display two to seven parvalbumin isotypes that are species specific and thus constitute a valuable tool in the study of phylogenetic relationships, and as a specific biomarker for fish identification. Parvalbumins were isolated from a selected sample of marine teleosts and elasmobranchs. Purified isotypes were characterized via HPLC (gel filtration, and ion exchange), and electrophoresis (IEF, and SDS-PAGE). An indirect immunoassay was developed for the parvalbumin isotypes using monoclonal antibodies directed against the highly conserved calcium-binding site. Parvalbumins from selected fish species are compared and contrasted by standard biochemical and immunological methods.
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Date Issued
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1998
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PURL
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http://purl.flvc.org/fcla/dt/15536
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Subject Headings
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Fishes, Calcium-binding proteins, Globular proteins
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Format
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Document (PDF)
