Current Search:  Biosynthesis (x)

View All Items

  • CSV Spreadsheet
(1 - 6 of 6)
Sterols of the marine sponge Petrosia weinbergi: implications for the absolute configurations of the antiviral orthoesterols and weinbersterols
Title
Sterols of the marine sponge Petrosia weinbergi: implications for the absolute configurations of the antiviral orthoesterols and weinbersterols.
Creator
Giner, José-Luis, Gunasekera, Sarath P., Pomponi, Shirley A.
Date Issued
1999
PURL
http://purl.flvc.org/FCLA/DT/3158771
Subject Headings
Sponges, Sterols --Analysis, Steroids, Biosynthesis, Marine metabolites
Format
Document (PDF)
Tubercidin, A Cytotoxic Agent from the Marine Sponge Caulospongia biflabellata
Title
Tubercidin, A Cytotoxic Agent from the Marine Sponge Caulospongia biflabellata.
Creator
Biabani, Misbah F., Gunasekera, Sarath P., Longley, Ross E., Wright, Amy E., Pomponi, Shirley A.
Date Issued
2002
PURL
http://purl.flvc.org/FCLA/DT/2795916
Subject Headings
Demospongiae, Nucleosides --chemistry, Cytotoxins --biosynthesis --congresses, Streptomyces, Antitumor antibiotics
Format
Document (PDF)
Exploration of Methods to Identify Biosynthetic Genes and the Origin of Biosynthesis in Marine Octocorals
Title
Exploration of Methods to Identify Biosynthetic Genes and the Origin of Biosynthesis in Marine Octocorals.
Creator
Ranzer, Llanie Karen, Florida Atlantic University, Kerr, Russell G., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
Abstract/Description
The overall goal of this research was to isolate key genes involved in the diterpene biosynthesis from Euniceafusca and Erythropodium caribaeorum using molecular biology techniques. The initial goal was to use fuscol induced cell cultures of Symbiodinium sp. isolated from E. fusca and to develop an approach based on differential display of mRNA-reverse transcription-PeR. Together with inverse PCR, these techniques ultimately provided a full-length farnesyl diphosphate synthase sequence....
Show moreThe overall goal of this research was to isolate key genes involved in the diterpene biosynthesis from Euniceafusca and Erythropodium caribaeorum using molecular biology techniques. The initial goal was to use fuscol induced cell cultures of Symbiodinium sp. isolated from E. fusca and to develop an approach based on differential display of mRNA-reverse transcription-PeR. Together with inverse PCR, these techniques ultimately provided a full-length farnesyl diphosphate synthase sequence. Functional expression of this enzyme was demonstrated with the addition of appropriate substrates and confirmed by chromatography. From this data, degenerate primer based PCR was used to isolate putative geranylgeranyl diphosphate biosynthetic genes from E. caribaeorum. Both chemical and genetic examinations of Pseudopterogorgia elisabethae eggs and their associated Symbiodinium sp. were employed to identify the biosynthetic origin of their diterpenes. Terpene content and biosynthetic capabilities of azooxanthellae eggs demonstrated the presence of pseudopterosins but also indicated that the eggs were not capable of producing these compounds. Likewise, no correlation could be observed for the phylogenetic relationships inferred for the Symbiodinium sp., with that of the terpene chemistry present in P. elisabethae. This finding leads us to speculate about an additional source of terpene production within this coral. Based on these and other recent findings suggesting symbiotic bacteria as the source of secondary metabolites from marine invertebrates, bacterial assemblages from E. caribaeorum were examined. This study revealed considerable phylogenetic bacterial diversity within this coral and the identification of several bacteria known to produce terpenes in other organisms.
Show less
Date Issued
2006
PURL
http://purl.flvc.org/fau/fd/FA00000874
Subject Headings
Coral reef ecology, Diterpenes, Biosynthesis, Terpenes--Synthesis
Format
Document (PDF)
Isolation, Characterization and Synthesis of New Diterpenes from Pseudopterogorgia elisabethae
Title
Isolation, Characterization and Synthesis of New Diterpenes from Pseudopterogorgia elisabethae.
Creator
Wan, Zhongliang, Florida Atlantic University, Kerr, Russell G., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
Abstract/Description
Pseudopterogorgia elisabethae is a known source of structurally interesting bioactive metabolites. A detailed search for new, related compounds was undertaken in this study which resulted in the isolation and characterization of more than ten new diterpenes with serrulatane and ileabethane skeletons. Some of the new compounds isolated are closely related terpenes with significant biological activity and others are likely to be key biosynthetic intermediates. As a component of the development...
Show morePseudopterogorgia elisabethae is a known source of structurally interesting bioactive metabolites. A detailed search for new, related compounds was undertaken in this study which resulted in the isolation and characterization of more than ten new diterpenes with serrulatane and ileabethane skeletons. Some of the new compounds isolated are closely related terpenes with significant biological activity and others are likely to be key biosynthetic intermediates. As a component of the development of a production method of anti-inflammatory compounds such as seco-pseudopterosin and elisabethadione, a synthesis of a seco-pseudoperosin aglycone from elisabethatriene was developed.
Show less
Date Issued
2006
PURL
http://purl.flvc.org/fau/fd/FA00000883
Subject Headings
Biosynthesis, Diterpenes--Synthesis, Marine invertebrates, Anti-inflammatory agents
Format
Document (PDF)
Origin and mechanism of terpene biosynthesis in Pseudopterogorgia spp
Title
Origin and mechanism of terpene biosynthesis in Pseudopterogorgia spp.
Creator
Boehnlein, Jennifer M., Florida Atlantic University, Kerr, Russell G.
Abstract/Description
The goal of this work was to investigate the biosynthetic origins of diterpene natural products (pseudopterosins, kallolides, bipinnatins, and cembrenes) from corals of the genus Pseudopterogorgia as well as the biosynthetic pathways by which they are produced. These studies have shown that the pseudopterosins from Pseudopterogorgia elisabethae are biosynthesized within the algal symbiont (or possibly a bacterium or fungus associated with the symbiont), are not inducible by manipulation of...
Show moreThe goal of this work was to investigate the biosynthetic origins of diterpene natural products (pseudopterosins, kallolides, bipinnatins, and cembrenes) from corals of the genus Pseudopterogorgia as well as the biosynthetic pathways by which they are produced. These studies have shown that the pseudopterosins from Pseudopterogorgia elisabethae are biosynthesized within the algal symbiont (or possibly a bacterium or fungus associated with the symbiont), are not inducible by manipulation of light levels, and do not change as a result of transplantation to new locations. Studies on Pseudopterogorgia bipinnata revealed that only one chemotype is capable of biosynthesizing the kallolide family of diterpenes. The biosynthetic pathway which gives rise to the kallolides has been shown to involve members of another family of diterpenes, the bipinnatins, which coexist within the coral holobiont. Two diterpene cyclase products have been discovered within P. bipinnata chemotype A, cembrene and neocembrene, and it has been shown that neocembrene gives rise to the kallolides. Finally, the enzymatic conversion of bipinnatin J to kallolide A has shown for the first time that these compounds are in fact biogenetically related.
Show less
Date Issued
2006
PURL
http://purl.flvc.org/fcla/dt/12205
Subject Headings
Terpenes--Synthesis, Marine pharmacology, Alcyonacea, Biosynthesis, Anti-inflammatory agents
Format
Document (PDF)
Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts
Title
Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts.
Creator
Zhang, Chi, Jia, Pingping, Huang, Xupei, Sferrazza, Gian Franco, Athauda, Gagani, Achary, Mohan P., Wang, Jikui, Lemanski, Sharon L., Lemanski, Larry F.
Date Issued
2009-09-03
PURL
http://purl.flvc.org/fcla/dt/3327272
Subject Headings
Ambystoma mexicanum --embryology, Ambystoma mexicanum --genetics, Base Sequence, DNA, Complementary --genetics, Endoderm --physiology, Exons --genetics, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, Genes, Recessive, Heart --embryology, Models, Genetic, Molecular Sequence Data, Muscle Proteins --biosynthesis, Muscle Proteins --genetics, Myocardial Contraction --physiology, Protein Isoforms, Reverse Transcriptase Polymerase Chain Reaction, Tropomyosin
Format
Document (PDF)