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DEVELOPMENT OF A BIOSENSOR FOR OBJECTIVELY QUANTIFYING NUISANCE ODORS NEAR LANDFILLS
Title
DEVELOPMENT OF A BIOSENSOR FOR OBJECTIVELY QUANTIFYING NUISANCE ODORS NEAR LANDFILLS.
Creator
Rahman, Sharmily, Meeroff, Daniel E., Florida Atlantic University, Department of Civil, Environmental and Geomatics Engineering, College of Engineering and Computer Science
Abstract/Description
Nuisance odors from landfills have more impact than just being an annoyance to nearby residents. With an ever-increasing population, a larger number of communities are located in closer proximity to landfills than ever before. This has brought along with it, more regular conflicts with landfill authorities surrounding the issue of odors, resulting in complaints, lawsuits, fines, and even re-siting operations. The absence of an objective method of quantifying nuisance odors makes the task of...
Show moreNuisance odors from landfills have more impact than just being an annoyance to nearby residents. With an ever-increasing population, a larger number of communities are located in closer proximity to landfills than ever before. This has brought along with it, more regular conflicts with landfill authorities surrounding the issue of odors, resulting in complaints, lawsuits, fines, and even re-siting operations. The absence of an objective method of quantifying nuisance odors makes the task of creating regulations and setting standards even more complicated. The current research focuses on a method to objectively quantify landfill odors. The human odorant binding protein 2A (hOBPIIa) can be produced using published recombinant gene technology and can be used as a biosensor to quantify odorants through spectrofluorometric measurements. The current work is a continuation of the previous work by Rahman (2020). In this work, the spent biosensor after it reacts with an odorant is shown to be regenerated by applying additional fluorophore following La Chateliers’ principle, so that the same batch of protein can be used to run multiple experiments with odorants. An important part of the work miniaturized the earlier version of the experimental setup and incorporates a much more efficient flow-through system. This setup is capable of collecting real-time readings, increasing the overall accuracy and shortening the duration of each set of the experiment. The current work also explores the response of the biosensor with an expanded group of pure odorants, including hydrogen sulfide, ammonia, toluene, formaldehyde, tert-butyl mercaptan, and methyl mercaptan as well as their mixtures, thus expanding the list of odorants tested under this principle. The results show that the protein shows a concentration-dependent response differing on the hydrophobicity of the target compound.
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Date Issued
2022
PURL
http://purl.flvc.org/fau/fd/FA00014101
Subject Headings
Landfills, Fills (Earthwork), Odors, Biosensors
Format
Document (PDF)
Immunoassay test strip for Microcystin-LR detection
Title
Immunoassay test strip for Microcystin-LR detection.
Creator
Xu, Jiesi., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
Abstract/Description
Microcystin-LR (MCLR) is hepatotoxic to animals and humans with disruption of liver structure causing cytoskeletal damage, necrosis and pooling of blood in the liver, leading to large increase in liver weight. It is also a strong liver tumor promoter and protein phosphatase inhibitor. Microcysin-LR binds protein phosphatases 1 and 2A, and influences regulation of cellular protein phosphorylation. In the present study, a colloidal gold based immunoassay test strip was developed for Microcystin...
Show moreMicrocystin-LR (MCLR) is hepatotoxic to animals and humans with disruption of liver structure causing cytoskeletal damage, necrosis and pooling of blood in the liver, leading to large increase in liver weight. It is also a strong liver tumor promoter and protein phosphatase inhibitor. Microcysin-LR binds protein phosphatases 1 and 2A, and influences regulation of cellular protein phosphorylation. In the present study, a colloidal gold based immunoassay test strip was developed for Microcystin-LR detection. The detection limit was found to be 1 ng/mL. 5 nm colloidal gold test strips exhibits more efficient for detection, compared with 20 nm colloidal gold test strips. The interaction between Microcystin-LR antibody (immunoglobulin G) and colloidal gold nanoparticles was investigated by various analytical methods, including Ultraviolet/Visible (UV/VIS), Fourier Transform Infrared (FTIR) and Fluorescence spectroscopy as well as transmission electron microscopy (TEM).
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Date Issued
2010
PURL
http://purl.flvc.org/FAU/2683532
Subject Headings
Immunoassay, Biosensors, Environmental chemistry, Cyanobacterial toxins, Drinking water, Microbiology
Format
Document (PDF)
DEVELOPMENT OF A BIOSENSOR FOR OBJECTIVELY QUANTIFYING ODORANTS
Title
DEVELOPMENT OF A BIOSENSOR FOR OBJECTIVELY QUANTIFYING ODORANTS.
Creator
Rahman, Sharmily, Meeroff, Daniel E., Florida Atlantic University, Department of Civil, Environmental and Geomatics Engineering, College of Engineering and Computer Science
Abstract/Description
Nuisance odor levels produced by solid waste management operations are subject to regulatory standards due to their impacts on the quality of life of the residents living nearby the facility. Failure to meet regulatory standards may result in fines, litigation, inability to acquire permits, mitigation, and re-siting operations. Since measurement of environmental nuisance odors is currently limited to subjective techniques, monitoring odor levels to meet such standards is often problematic....
Show moreNuisance odor levels produced by solid waste management operations are subject to regulatory standards due to their impacts on the quality of life of the residents living nearby the facility. Failure to meet regulatory standards may result in fines, litigation, inability to acquire permits, mitigation, and re-siting operations. Since measurement of environmental nuisance odors is currently limited to subjective techniques, monitoring odor levels to meet such standards is often problematic. This is becoming more acute as increasing residential populations begin to encroach on properties adjacent to landfills. In order to ensure that nuisance odor issues are minimized, it is necessary to provide an objective measurement. The objective of the current research is to develop a biosensor for providing an objective, standard measurement of odors. The approach is to modify the human odorant binding protein (hOBPIIa), isolated using published biomolecular techniques, by fluorescently tagging it with a chromophore functional group. When this protein is tagged with a fluorophore marker and excited in a spectrofluorometer, it emits light of a certain wavelength that can be detected and quantified. Once odorant molecules are exposed to this complex, they start replacing the fluorophore, and as a result, the emitted light intensity decreases in proportion to the number of odorant molecules. Since the protein response depends on odorant concentration, following an inverse Beer’s Law relationship, the odorants can be quantified accurately and rapidly using fluorometric measurements. The results establish quantitation ranges for different pure and mixture of odorant gases as well as the amount of gas that can be quantified across various flow rates.
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Date Issued
2020
PURL
http://purl.flvc.org/fau/fd/FA00013491
Subject Headings
Biosensors, Odors--Measurement, Landfills, Odorant-binding protein, Fluorescence--Measurement
Format
Document (PDF)
Development of a Biosensor to Detect Landfill Odors Using Human Odorant Binding Protein
Title
Development of a Biosensor to Detect Landfill Odors Using Human Odorant Binding Protein.
Creator
Roblyer, Julia Gia, Meeroff, Daniel E., Florida Atlantic University, College of Engineering and Computer Science, Department of Civil, Environmental and Geomatics Engineering
Abstract/Description
Landfills receive odor complaints from local residents potentially resulting in legal ramifications and decreasing quality of life of the neighboring communities. However, analytical technologies to objectively measure odors in the field that will reflect human odor perceptions at a reasonable price do not currently exist. Hydrogen sulfide (H2S) is detectable by humans at low odor thresholds and may be one of the main nuisance odors emanating from landfills. For the first time, hOBPIIa...
Show moreLandfills receive odor complaints from local residents potentially resulting in legal ramifications and decreasing quality of life of the neighboring communities. However, analytical technologies to objectively measure odors in the field that will reflect human odor perceptions at a reasonable price do not currently exist. Hydrogen sulfide (H2S) is detectable by humans at low odor thresholds and may be one of the main nuisance odors emanating from landfills. For the first time, hOBPIIa complexed with 1- AMA has been used to detect H2S concentrations by spectrofluorometry. The optimal ratio of hOPBIIa-to-1-AMA was determined to be approximately 1:1. A strong linear regression model was generated to predict H2S concentrations from peak fluorescent emission intensity measurements within a range of 0-8.3 g of H2S. A novel experimental exposure chamber prototype was developed that has the potential to be incorporated into a portable sensor because it is compact.
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Date Issued
2017
PURL
http://purl.flvc.org/fau/fd/FA00005939
Subject Headings
Dissertations, Academic -- Florida Atlantic University, Landfills., Biosensors., Odor sensors., Odor control.
Format
Document (PDF)
Rapid Analysis of Fecal Glucocorticoid Metabolites: Testing an Alternative Method for Analyzing Stress Markers in Chimpanzees
Title
Rapid Analysis of Fecal Glucocorticoid Metabolites: Testing an Alternative Method for Analyzing Stress Markers in Chimpanzees.
Creator
Litman, Benni, Broadfield, Douglas C., Harris, Michael S., Florida Atlantic University, Dorothy F. Schmidt College of Arts and Letters, Department of Anthropology
Abstract/Description
This study explores the application of two methods of spectroscopy; Near Infrared spectroscopy (NIR) and Fourier transform spectroscopy (FTIR) as alternative approaches for measuring glucocorticoid metabolites in chimpanzee feces. The goals of this study were twofold: The first was to determine if cortisol can be identified within the NIR and/or FTIR spectra of chimpanzee fecal hormone extract in ethanol solution. The second objective was to determine the capability of NIR and FTIR to predict...
Show moreThis study explores the application of two methods of spectroscopy; Near Infrared spectroscopy (NIR) and Fourier transform spectroscopy (FTIR) as alternative approaches for measuring glucocorticoid metabolites in chimpanzee feces. The goals of this study were twofold: The first was to determine if cortisol can be identified within the NIR and/or FTIR spectra of chimpanzee fecal hormone extract in ethanol solution. The second objective was to determine the capability of NIR and FTIR to predict FGM concentrations obtained using standard laboratory methods. Fecal glucocorticoid concentrations measured by Enzyme Immunoassay were used as the reference data of partial least square (PLS) regression of fecal extract NIR spectra and FTIR spectra. Low accuracies (NIR: R2 = 0.152; FTIR: R2 = 0.199) were obtained from regression models using data from both methods. Though this study did not successfully demonstrate the feasibility of using NIR and FTIR to qualify and quantify FGMs, it is likely not a reflection of the capabilities of the technology, but rather of appropriate sample types and preparation methods.
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Date Issued
2016
PURL
http://purl.flvc.org/fau/fd/FA00004723, http://purl.flvc.org/fau/fd/FA00004723
Subject Headings
Biosensors, Infrared spectroscopy, Metabolites -- Spectra, Primates as laboratory animals, Spectrum analysis -- Diagnostic use
Format
Document (PDF)
SUPPORTED ERYTHROCYTE MEMBRANES ON PIEZOELECTRIC SENSORS FOR STUDYING THE INTERACTIONS BETWEEN NANOPARTICLES AND SURFACES OF RED BLOOD CELLS
Title
SUPPORTED ERYTHROCYTE MEMBRANES ON PIEZOELECTRIC SENSORS FOR STUDYING THE INTERACTIONS BETWEEN NANOPARTICLES AND SURFACES OF RED BLOOD CELLS.
Creator
Islam, Tanaz, Yi, Peng, Florida Atlantic University, Department of Civil, Environmental and Geomatics Engineering, College of Engineering and Computer Science
Abstract/Description
The Supported red blood cell membrane (SRBCm) was developed on a piezoelectric sensor to study the attachment of nanoparticles to erythrocyte surfaces. A well-dispersed colloidal suspension of fragments of RBCm was prepared from whole blood, and characterized thoroughly using cryogenic transmission electron microscopy, dynamic light scattering, and zeta potential analysis. To develop SRBCm, RBCm fragments were immobilized onthe sensor in a quartz crystal microbalance with dissipation...
Show moreThe Supported red blood cell membrane (SRBCm) was developed on a piezoelectric sensor to study the attachment of nanoparticles to erythrocyte surfaces. A well-dispersed colloidal suspension of fragments of RBCm was prepared from whole blood, and characterized thoroughly using cryogenic transmission electron microscopy, dynamic light scattering, and zeta potential analysis. To develop SRBCm, RBCm fragments were immobilized onthe sensor in a quartz crystal microbalance with dissipation monitoring system. A complete monolayer of flattened fragments of RBCm was formed on the positively charged surface of the piezoelectric sensor in 1 mM NaCl and 0.2 mM NaHCO3 at pH 7.1. The surface morphology of SRBCm was characterized via atomic force microscopy. The even distribution of surface proteins expressed on erythrocytes was found on SRBCm through indirect immunofluorescence microscopy. The attachment efficiencies of model nanoparticles, e.g. hematite nanoparticles and carboxylated polystyrene nanoparticles, on the SRBCm were quantified using a classic methodology. KEYWORDS: Supported erythrocyte membrane, piezoelectric sensor, phospholipid bilayers, nanoparticles
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Date Issued
2019
PURL
http://purl.flvc.org/fau/fd/FA00013383
Subject Headings
Erythrocyte Membrane, Piezoelectric polymer biosensors, Nanoparticles, Phospholipid bilayers, Bilayer lipid membranes
Format
Document (PDF)
Noninvasive Blood-Glucose Monitoring: A Microwave-based Biosensor Development
Title
Noninvasive Blood-Glucose Monitoring: A Microwave-based Biosensor Development.
Creator
Shankar, Poornima, Neelakanta, Perambur S., Florida Atlantic University, College of Engineering and Computer Science, Department of Computer and Electrical Engineering and Computer Science
Abstract/Description
This research refers to a proof-of-concept study concerning the development of a noninvasive blood-glucose monitoring system. The biosensor being considered is a microwave-based transducer (that can be rendered compatible for ISM band of 2450 MHz and hence Zigbee™ and/or BluetoothTM compliant). The goal of this study is tailored to develop eventually a unit for home-based healthcare and/or personalized wellness monitoring of diabetic patients. This pilot effort is expected to culminate in...
Show moreThis research refers to a proof-of-concept study concerning the development of a noninvasive blood-glucose monitoring system. The biosensor being considered is a microwave-based transducer (that can be rendered compatible for ISM band of 2450 MHz and hence Zigbee™ and/or BluetoothTM compliant). The goal of this study is tailored to develop eventually a unit for home-based healthcare and/or personalized wellness monitoring of diabetic patients. This pilot effort is expected to culminate in future in a wireless hyper/hypoglycemic risk-alert system and possible automatic insulin infusion pump activation efforts. The thesis addressed thereof provides details on the fundamentals of sensing glucose content noninvasively across a finger. The underlying principle of biosensing refers to detecting the change in the dielectric property of blood with differential changes in the glucose influx in the finger by sensing microwave (such as 2450 MHz) absorption and/or reflection so as to assay the glucose content of interest. Preliminary experimental and theoretical results are presented and discussed.
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Date Issued
2008
PURL
http://purl.flvc.org/fau/fd/FA00012552
Subject Headings
Bioelectrochemistry--Research, Blood sugar monitoring, Diabetes--Risk factors--Identification, Biosensors--Materials, Microwave transmission lines, Microwave integrated circuits
Format
Document (PDF)