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(1 - 5 of 5)
- Title
- Effects of anoxia on methionine sulfoxide reductase (Msr) deficient drosophila.
- Creator
- Howard, Danielle, Binninger, David
- Date Issued
- 2013-04-05
- PURL
- http://purl.flvc.org/fcla/dt/3361093
- Subject Headings
- Anoxia, Drosophila, Reactive Oxygen Species
- Format
- Document (PDF)
- Title
- Origin of glutamate release in the normoxic, anoxic and ischemic isolated turtle cerebellum (Trachemys scripta).
- Creator
- Thompson, John William., Florida Atlantic University, Lutz, Peter L.
- Abstract/Description
-
The uncontrolled release of glutamate is thought to be a key event in the death of the anoxic/ischemic mammalian brain. However the origin of glutamate, vesicular and/or cytoplasmic, is unknown. Likewise, the anoxic turtle releases a surge of glutamate upon anoxic depolarization. Therefore, this study investigated the origin of glutamate release in the isolated cerebellum of the freshwater turtle (Trachemys scripta). The results showed that: during anoxia, low extracellular glutamate levels...
Show moreThe uncontrolled release of glutamate is thought to be a key event in the death of the anoxic/ischemic mammalian brain. However the origin of glutamate, vesicular and/or cytoplasmic, is unknown. Likewise, the anoxic turtle releases a surge of glutamate upon anoxic depolarization. Therefore, this study investigated the origin of glutamate release in the isolated cerebellum of the freshwater turtle (Trachemys scripta). The results showed that: during anoxia, low extracellular glutamate levels are maintained, an ability lost during in vitro ischemia, by a reduction in glutamate release. Upon anoxic depolarization glutamate release originates from the cytoplasm. The lack of vesicular glutamate release is likely the result of an inhibition of vesicular exocytosis. However during in vitro ischemia, the inhibitory signal which blocks vesicular glutamate release during anoxia is lost. This allows a biphasic pattern of glutamate release during ischemia originating initially from vesicular stores which then causes cytoplasmic glutamate release.
Show less - Date Issued
- 2001
- PURL
- http://purl.flvc.org/fcla/dt/12803
- Subject Headings
- Trachemys scripta, Turtles, Cerebral anoxia
- Format
- Document (PDF)
- Title
- Ion homeostasis and energy loss in the anoxic frog brain (Rana pipiens).
- Creator
- Knickerbocker, Debra L., Florida Atlantic University, Lutz, Peter L., Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
This study investigated the homeostasis of extracellular potassium ion concentration ([K+]e) and correlated energy levels in the anoxic frog brain (Rana pipiens). The results showed K+ ion homeostasis was maintained for 3hrs in anoxia. It confirmed a constant decline in brain ATP in anoxia. It was concluded that ion homeostasis in the frog does not rely on the maintenance of normoxic levels of ATP. When Na+/K+ATPase was inhibited, it was found that initial K+ efflux was decreased in the...
Show moreThis study investigated the homeostasis of extracellular potassium ion concentration ([K+]e) and correlated energy levels in the anoxic frog brain (Rana pipiens). The results showed K+ ion homeostasis was maintained for 3hrs in anoxia. It confirmed a constant decline in brain ATP in anoxia. It was concluded that ion homeostasis in the frog does not rely on the maintenance of normoxic levels of ATP. When Na+/K+ATPase was inhibited, it was found that initial K+ efflux was decreased in the anoxic frog. This could be due to decreased ion permeability in anoxia, an adaptive strategy seen in other anoxic tolerant organisms. However, the study showed once a threshold [K+]e was reached, the final K + efflux pattern was unchanged. This [K+]e appears to represent a membrane potential threshold. Critical energy levels were determined representing the energy minimum to maintain ion homeostasis in the anoxic frog brain.
Show less - Date Issued
- 2000
- PURL
- http://purl.flvc.org/fcla/dt/12717
- Subject Headings
- Northern leopard frog, Cerebral anoxia
- Format
- Document (PDF)
- Title
- Eco-physiology of juvenile snook Centropomus undecimalis (Bloch): life-history implications.
- Creator
- Peterson, Mark S., Gilmore, R. G., Harbor Branch Oceanographic Institute
- Date Issued
- 1991
- PURL
- http://purl.flvc.org/FCLA/DT/3171646
- Subject Headings
- Snook, Fishes --Habitat, Hematocrit, Hypoxia (Water), Anoxia
- Format
- Document (PDF)
- Title
- The Effects of MsrA and MsrB in Anoxia Tolerance in Aging Drosophila melanogaster.
- Creator
- Suthakaran, Nirthieca, Binninger, David, Florida Atlantic University, Charles E. Schmidt College of Medicine, Department of Biomedical Sciences
- Abstract/Description
-
Drosophila melanogaster tolerates several hours of anoxia (the absence of oxygen) by entering a protective coma. A burst of reactive oxygen species (ROS) is produced when oxygen is reintroduced to the cells. ROS causes oxidative damage to critical cellular molecules, which contribute to aging and development of certain agerelated conditions. The amino acid, methionine, is susceptible to oxidation, although this damage can be reversed by methionine sulfoxide reductases (Msr). This project...
Show moreDrosophila melanogaster tolerates several hours of anoxia (the absence of oxygen) by entering a protective coma. A burst of reactive oxygen species (ROS) is produced when oxygen is reintroduced to the cells. ROS causes oxidative damage to critical cellular molecules, which contribute to aging and development of certain agerelated conditions. The amino acid, methionine, is susceptible to oxidation, although this damage can be reversed by methionine sulfoxide reductases (Msr). This project investigates the effect of Msr-deficiency on anoxia tolerance in Drosophila throughout the lifespan of the animal. The data show that the time for recovery from the protective comma as well as the survival of the animals lacking any Msr activity depends on how quickly the coma is induced by the anoxic conditions. Insight into the roles(s) of Msr genes under anoxic stress can lead us to a path of designing therapeutic drugs around these genes in relation to stroke.
Show less - Date Issued
- 2018
- PURL
- http://purl.flvc.org/fau/fd/FA00013046
- Subject Headings
- Drosophila melanogaster, Methionine Sulfoxide Reductases, Anoxia, Aging, Oxidative stress
- Format
- Document (PDF)
