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Immunochemical Behavior and Isolation of Equine Hemagglutinins Found in Normal and Hyperimmune Serum
Title
Immunochemical Behavior and Isolation of Equine Hemagglutinins Found in Normal and Hyperimmune Serum.
Creator
Monkus, Thomas P., Saurino, Vincent R., Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description
In studying Equine Infectious Anemia (EIA), a specific viral disease of Equidae, characterized by intermittent fever, anemia and progressive weakness, questions have arisen as to the existence of a viral hemagglutinin. Raised hemagglutinin levels are present in the infected sera, but this is further complicated by the fact that some horse serums contain relatively high levels of hemagglutinins which are believed to be non-specific natural agglutinins. This investigation, which parallels...
Show moreIn studying Equine Infectious Anemia (EIA), a specific viral disease of Equidae, characterized by intermittent fever, anemia and progressive weakness, questions have arisen as to the existence of a viral hemagglutinin. Raised hemagglutinin levels are present in the infected sera, but this is further complicated by the fact that some horse serums contain relatively high levels of hemagglutinins which are believed to be non-specific natural agglutinins. This investigation, which parallels research currently in progress on the EIA virus proper, is designed to characterize those agglutinins found in non-infected sera. Two principal types of equine hemagglutinins were studied; (1) the natural occurring non-specific blood agglutinin, and (2) hyperimmune isoagglutinins produced in a primary immune response following injection of heterogenic equine erythrocytes. Both species of agglutinins were obtained from whole serum. Standard immunological techniques such as reduction and alkylation, heat inactivation, ion-exchange chromotography, density gradient ultracentrifugation, and electrophoresis were employed in the investigation of structural and biological characteristics of the hemagglutinins. The results have shown that natural equine hemagglutinins are heterospecific. They are sensitive to heat (56°C) and reducing agents such as mercaptoethanol. Natural agglutinins have a sedimentation coefficient of 18.65 and electrophoretically migrate as alpha2 globulins. The hyperimmune isohemagglutinin is a specific antibody reactive with a well defined antigen. It is relatively heat stabile and hemagglutinating activity is reduced with mercaptoethanol. Similarly, the isoagglutinin has the some sedimentation coefficient and electrophoretic mobility as the natural antibody. The isoagglutinin is unique in that it represents a new class of antibody not previously reported.
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Date Issued
1969
PURL
http://purl.flvc.org/fau/fd/FA00000800
Subject Headings
Equine infectious anemia, Agglutinins
Format
Document (PDF)
Agglutinins from marine macroalgae of the southeastern United States
Title
Agglutinins from marine macroalgae of the southeastern United States.
Creator
Bird, Kimon T., Chiles, Thomas C., Longley, Ross E., Kendrick, A. F., Kinkema, M. D., Harbor Branch Oceanographic Institute
Date Issued
1993
PURL
http://purl.flvc.org/FCLA/DT/3333208
Subject Headings
Agglutinins, Marine algae, Lectins, Mitogens, Lymphocytes
Format
Document (PDF)
A comparative study of animal erythrocyte agglutinins from marine algae
Title
A comparative study of animal erythrocyte agglutinins from marine algae.
Creator
Chiles, Thomas C., Bird, Kimon T., Harbor Branch Oceanographic Institute
Date Issued
1989
PURL
http://purl.flvc.org/FCLA/DT/3353838
Subject Headings
Marine algae, Agglutinins, Erythrocytes, Glycoproteins, Polysaccharides, Monosaccharides, Comparative Study
Format
Document (PDF)