Current Search: Stress (x)
Pages
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Title
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Effects of stressors on differential gene expression and secondary metabolites by Axinella corrugata.
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Creator
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Grima, Jennifer., Charles E. Schmidt College of Science, Department of Biological Sciences
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Abstract/Description
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Sponges are an important source of bioactive marine natural products, or secondary metabolites. The common Caribbean reef sponge, Axinella corrugata, produces an antitumor and antibacterial chemical, stevensine. This study determined whether environmental stressors, such as elevated temperature and exposure to Amphibalanus amphitrite larvae, affect the production of stevensine by A.corrugata and if the stressors caused A.corrugata to exhibit differential gene expression. Temperature stress...
Show moreSponges are an important source of bioactive marine natural products, or secondary metabolites. The common Caribbean reef sponge, Axinella corrugata, produces an antitumor and antibacterial chemical, stevensine. This study determined whether environmental stressors, such as elevated temperature and exposure to Amphibalanus amphitrite larvae, affect the production of stevensine by A.corrugata and if the stressors caused A.corrugata to exhibit differential gene expression. Temperature stress resulted in no significant change in the production of stevensine; only two genes were significantly differentially expressed, including hsp70. Larval stressed resulted in increased production of stevensine and significant differential gene expression (more than seventy genes). This study suggests that A.corrugata may be resilient to elevations in temperature and that one of stevensine's roles in nature is as an antifoulant.
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Date Issued
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2013
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PURL
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http://purl.flvc.org/fcla/dt/3360781
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Subject Headings
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Axinellida, Sponges, Marine pharmacology, Adaptation (Biology), Gene expression, Genetic regulation, Stress (Physiology), Ecophysiology
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Format
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Document (PDF)
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Title
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Fatigue and fracture of foam cores used in sandwich composites.
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Creator
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Saenz, Elio., College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
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Abstract/Description
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This study focused on the fracture and fatigue crack growth behavior in polyvinylchloride (PVC) and polyethersulfone (PES) foams. A new sandwich double cantilever beam (DCB) test specimen was implemented. Elastic foundation and finite element analysis and experimental testing confirmed that the DCB specimen is appropriate for static and cyclic crack propagation testing of soft polymer foams. A comprehensive experimental mechanical analysis was conducted on PVC foams of densities ranging from...
Show moreThis study focused on the fracture and fatigue crack growth behavior in polyvinylchloride (PVC) and polyethersulfone (PES) foams. A new sandwich double cantilever beam (DCB) test specimen was implemented. Elastic foundation and finite element analysis and experimental testing confirmed that the DCB specimen is appropriate for static and cyclic crack propagation testing of soft polymer foams. A comprehensive experimental mechanical analysis was conducted on PVC foams of densities ranging from 45 to 100 kg/m3 and PES foams of densities ranging from 60 to 130 kg/m3. An in-situ scanning electron microscope study on miniature foam fracture specimens showed that crack propagation in the PVC foam was inter-cellular and in the PES foam, failure occurred predominately by extensional failure of vertical cell edges. Sandwich DCB specimens were loaded cyclically as well. For the PVC foams, the crack growth rates were substantially influenced by the density. For the PES foams, there was no clear indication about the influence of foam density on the crack growth rate.
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Date Issued
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2012
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PURL
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http://purl.flvc.org/FAU/3352829
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Subject Headings
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Sandwich construction, Composite materials, Fibrous composites, Strains and stresses, Management, Laminated materials, Plastics, Fatigue
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Format
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Document (PDF)
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Title
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Identifying meaningful types in daily life.
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Creator
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Thompson, Brittany M., Charles E. Schmidt College of Science, Department of Psychology
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Abstract/Description
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Despite long-held recognition of the importance of situations in psychological understanding and analysis, current research is lacking in discernment of structurally important elements of situations as they relate to behavior (Funder et al., 2012). Using the Riverside Situational Q-sort (RSQ: Wagerman & Funder, 2009), an 89-item measure used to assess the psychological properties of situations, the major aim of this study was to identify a reliable set of categories or types of situations...
Show moreDespite long-held recognition of the importance of situations in psychological understanding and analysis, current research is lacking in discernment of structurally important elements of situations as they relate to behavior (Funder et al., 2012). Using the Riverside Situational Q-sort (RSQ: Wagerman & Funder, 2009), an 89-item measure used to assess the psychological properties of situations, the major aim of this study was to identify a reliable set of categories or types of situations that people experience every day. Data was collected online from a U.S. sample (N = 186). Participants were asked to recall details about a situation he or she experienced during the previous day (i.e. "What were you doing yesterday at this time?"). Participants were then asked to rate that situation using the RSQ. Inverse factor analyses revealed the following everyday situation types: 1) Social Closeness, 2) Obligatory, 3) Cognitive, 4) Enjoyable/Aesthetic, and 5) Anxiety Inducing.
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Date Issued
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2013
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PURL
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http://purl.flvc.org/fcla/dt/3361252
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Subject Headings
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Conduct of life, Stress (Psychology), Social psychology, Interbehavioral psychology, Motivation (Psychology)
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Format
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Document (PDF)
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Title
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Inflammatory response in stress and the role of autophagy in breast cancer.
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Creator
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Onwuha-Ekpete, Lillian C., Charles E. Schmidt College of Medicine, Department of Biomedical Science
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Abstract/Description
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We attempted to understand the molecular regulators that impact inflammation using a rat model of human sensation-seeking/risk-taking trait for drug and stress vulnerability, based on their exploratory behavior displaying high rates (HRs) or low rates of locomotor reactivity (LRs) to environmental stress. We found that HRs have a pro-inflammatory phenotype as indicated by increased protein expression of the inflammatory cytokine TNF-(Sa(B. Furthermore, we found that HRs have a lower gene...
Show moreWe attempted to understand the molecular regulators that impact inflammation using a rat model of human sensation-seeking/risk-taking trait for drug and stress vulnerability, based on their exploratory behavior displaying high rates (HRs) or low rates of locomotor reactivity (LRs) to environmental stress. We found that HRs have a pro-inflammatory phenotype as indicated by increased protein expression of the inflammatory cytokine TNF-(Sa(B. Furthermore, we found that HRs have a lower gene expression of the glucocorticoid receptor and histone deacetylase 2 which are known to play an immunosuppressive role. Autophagy (macroautophagy) is a homeostatic process needed for cell maintenance, growth and proliferation and known to assist in tumor survival. FYVE and coiled-coil domain containing 1 (FYCO1) is a novel protein implicated to assist in the plus-end directed trafficking and fusion of autophagosomes. In these studies, we show that FYCO1 gene expression among human breast cell lines of varying degrees of malignancy.
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Date Issued
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2012
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PURL
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http://purl.flvc.org/fcla/dt/3362042
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Subject Headings
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Breast, Cancer, Genetic aspects, Cancer, Molecular aspects, Carcinogenesis, Cellular signal transduction, Stress (Physiology)
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Format
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Document (PDF)
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Title
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The effect of engagement interventions on uncertainty and coping of Thai family members of patients in a coma.
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Creator
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Piyakong, Duangporn, Liehr, Patricia, Florida Atlantic University, Christine E. Lynn College of Nursing
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Abstract/Description
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The purpose of this study was to test the effect of the expressive writing and the expressive talking interventions on uncertainty and coping processes in Thai family members of patients in a coma, and to examine changes in positive emotion, negative emotion, and cognitive process word-use over three days. A quasi-experimental pretest-posttest, three group design was conducted with 45 Thai family members of patients in a coma who were in the intensive care units (ICU) at a hospital in...
Show moreThe purpose of this study was to test the effect of the expressive writing and the expressive talking interventions on uncertainty and coping processes in Thai family members of patients in a coma, and to examine changes in positive emotion, negative emotion, and cognitive process word-use over three days. A quasi-experimental pretest-posttest, three group design was conducted with 45 Thai family members of patients in a coma who were in the intensive care units (ICU) at a hospital in Thailand. Three ICUs were randomly assigned into groups: the expressive writing, the expressive talking, and the control group. Participants of the expressive writing group (n = 15) were asked to write about their thoughts and feelings following standard guidance for expressive writing, those of the expressive talking group (n = 15) were interviewed with questions guided by the story path approach, whereas those of the control group (n = 15) did not receive any specific interventions. Participants completed the Thai version of the Mishel Uncertainty in Illness Scale-Family Member Form and the Thai version of the revised version of the Ways of Coping Scale before and after the interventions.
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Date Issued
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2014
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PURL
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http://purl.flvc.org/fau/fd/FA00004318
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Subject Headings
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Adjustment (Psychology), Critically ill -- Family relationships, Intensive care units, Stress (Psychology)
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Format
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Document (PDF)
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Title
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The lived experience of the spouses of transplant recipients.
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Creator
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Stecher, Jo Anne., Christine E. Lynn College of Nursing
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Abstract/Description
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The numbers of transplant surgeries continue to rise in the U.S. This results in a greater number of caregivers who are present in the recipients' lives. Most often these caregivers are spouses. Transplant spouses present with emotional needs, which have been expressed in this study. Nurses, especially those who work with the spouses of transplant candidates and recipients, could be appreciative of the requirements that have been told by these spouses. Six women and two men were interviewed...
Show moreThe numbers of transplant surgeries continue to rise in the U.S. This results in a greater number of caregivers who are present in the recipients' lives. Most often these caregivers are spouses. Transplant spouses present with emotional needs, which have been expressed in this study. Nurses, especially those who work with the spouses of transplant candidates and recipients, could be appreciative of the requirements that have been told by these spouses. Six women and two men were interviewed for this study. Their stories were analyzed using a phenomenological approach and five themes emerged from the data. The themes that surfaced included uncertainty, support, thankfulness and a positive approach, intimacy and the relationship as a couple, and guilt. Two general structures then evolved from these themes to include adaptation and belief in self and others. The general structures revealed the ways the spouses learned to live during the transplant journey.
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Date Issued
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2009
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PURL
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http://purl.flvc.org/FAU/367765
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Subject Headings
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Transplantation of organs, tissues, etc, Family relationships, Caregivers, Conduct of life, Stress management
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Format
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Document (PDF)
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Title
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The role of identity in posttraumatic growth and psychological adjustment for adults with cancer.
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Creator
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Abernathy, Barbara E., College of Education, Department of Counselor Education
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Abstract/Description
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This mixed methods sequential research study was performed to explore the role of identity in posttraumatic growth and psychological adjustment for adults with cancer. One hundred nineteen individuals participated in an online survey which included items from Brief COPE, Mini-Mental Adjustment to Cancer (Mini-MAC), Brief Illness Perception Questionnaire (BIPQ), Sense of Coherence Scale - 3 items (SOC-3), Posttraumatic Growth Inventory (PTGI), Illness Intrusiveness Ratings Scale (IIRS), and...
Show moreThis mixed methods sequential research study was performed to explore the role of identity in posttraumatic growth and psychological adjustment for adults with cancer. One hundred nineteen individuals participated in an online survey which included items from Brief COPE, Mini-Mental Adjustment to Cancer (Mini-MAC), Brief Illness Perception Questionnaire (BIPQ), Sense of Coherence Scale - 3 items (SOC-3), Posttraumatic Growth Inventory (PTGI), Illness Intrusiveness Ratings Scale (IIRS), and Centrality of Event Scale (CES). A two-step cluster analysis divided the sample into two clusters based on the integration of cancer into identity: High Cancer Identity Cluster (cancer identity scores above M) with strong cancer identity and Low Cancer Identity Cluster (scores below the M) with a weak or absent cancer identity. HCIC yielded positive and negative subgroups. A discriminant analysis revealed which variables are significant predictors of group membership: PTG factor New Possibilities (Wilks'l = .781, F (1, 119) = 32.834, p = .000), Psychological Adjustment factor Anxious Preoccupation (Wilks' l= .863, F (1, 119) = 18.612, p = .000), Illness Intrusiveness factor Intimate Relationships (Wilks' l= .794, F (1, 119) = 30.348, p = .000), and Illness Perception factor Perceived Life Impact of Cancer (Wilks' l= .783, F (1, 119) = 32.412, p = .000). From the sample, 17 individuals and spouses/partners were interviewed to obtain a deeper understanding of the lived experience of cancer. Qualitative themes of suffering, woundedness, and uncertainty were found. Narrative data corroborated the quantitative data and contributed depth to the analysis. A new Cancer Identity Process Model was offered in which assimilative and accommodative efforts are informed by identity structures., Performing Normalcy is an assimilative process in which stressful life events such as cancer activate automatic behaviors guided by existing identity structures with the goal of reg As dissonance grows over the inability to re-establish valued former identities, negative affect and intrusive rumination prevails. Individuals then utilize accommodative strategies in a process of Constructing Survivorship to either regain valuable aspect of former identities or to create equally valued new ones.
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Date Issued
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2009
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PURL
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http://purl.flvc.org/FAU/187204
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Subject Headings
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Adjustment (Psychology), Cancer, Psychological aspects, Stress management, Identity (Psychology), Mind and body
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Format
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Document (PDF)
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Title
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The relationship bertween wellness and burnout among novice counselors.
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Creator
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Bilot, Jennifer., College of Education, Department of Counselor Education
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Abstract/Description
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The purpose of this research was to explore the relationship between wellness and burnout among novice counselors. This research was carried out in order to gain a better understanding of the experience of the novice counselor as well as to gain insight into the effects of wellness and burnout on these experiences. The significance of this research lies in the need to understand the novice counselor's journey, the factors that can contribute to or prevent burnout, and the potential prevention...
Show moreThe purpose of this research was to explore the relationship between wellness and burnout among novice counselors. This research was carried out in order to gain a better understanding of the experience of the novice counselor as well as to gain insight into the effects of wellness and burnout on these experiences. The significance of this research lies in the need to understand the novice counselor's journey, the factors that can contribute to or prevent burnout, and the potential prevention and intervention strategies that can be employed in response to burnout. The data of a final sample of 144 participants' scores on the Maslach Burnout Inventory (MBI), The Five Factor Wellness Evaluation of Lifestyle (5F-Wel), and a demographic questionnaire were analyzed using the Statistical Package for the Social Sciences in order to explore the relationships between wellness and burnout. The researcher found significant relationships between demographic factors of the novice counselor, including age, years of experience, and number of hours delivering counseling services, and both the MBI and the 5F-Wel. The researcher also found a relationship between all factors of wellness and the three subscales of burnout. A particularly important finding was the significance of the Coping Self, Physical Self, and Creative Self subscales of wellness to the subscales of burnout. The implications of the findings include the development of specific intervention and prevention strategies for novice counselors. Limitations of this research include the relatively small sample size, potential bias among the participants who chose to complete the survey, and the limited generalizability.
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Date Issued
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2012
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PURL
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http://purl.flvc.org/FAU/3355624
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Subject Headings
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Work, Psychological aspects, Burn out (Psychology), Prevention, Job stress, Clinical health psychology
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Format
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Document (PDF)
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Title
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The impacts of nest microenvironment on sea turtle hatchling performance and their responses to thermal stress.
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Creator
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Seaman, Heather A., Milton, Sarah, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
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Abstract/Description
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As climate change threatens with sea-level rise and more storms, increased erosion could increase the need for beach nourishment. Alterations to sand characteristics may result in changes to the sea turtle nest microenvironment, impacting the temperature and oxygen levels which may affect hatchling performance. In this study, leatherback, loggerhead, and green nests were sampled from two sites with different sand characteristics in Juno Beach, Florida, USA. Gas exchange was higher in green...
Show moreAs climate change threatens with sea-level rise and more storms, increased erosion could increase the need for beach nourishment. Alterations to sand characteristics may result in changes to the sea turtle nest microenvironment, impacting the temperature and oxygen levels which may affect hatchling performance. In this study, leatherback, loggerhead, and green nests were sampled from two sites with different sand characteristics in Juno Beach, Florida, USA. Gas exchange was higher in green turtle nests with a greater mixture of sediment. Darker sediment elevated nest temperatures. Finer sediment and a greater mixture of sediment in leatherback nests elevated the nest temperatures; conversely finer sediment, and a greater mixture of sediment decreased loggerhead and green nest temperatures. Elevated nest temperatures reduced leatherback, loggerhead, and green turtle hatchling performance. Understanding the relationships between beach composition, nest environment, and hatchling performance will aid management decisions essential to sea turtle conservation.
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Date Issued
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2020
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PURL
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http://purl.flvc.org/fau/fd/FA00013542
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Subject Headings
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Sea turtles--Nests, Thermal stress (Biology), Sea turtles--Conservation, Beach nourishment
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Format
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Document (PDF)
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Title
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The effects of the proactive personality on the levels of job satisfaction and burnout for licensed mental health counselors.
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Creator
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Spina, Pamela F., College of Education, Department of Counselor Education
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Abstract/Description
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Personal wellness and burnout have been common themes in research studies regarding professional satisfaction and career success. Personality characteristics in relation to job and career success among professionals have also been included in countless studies. However, Bateman and Crant (1993) defined and began research on the specific construct of the proactive personality and how it related to personal achievement, satisfaction, and success among executives. This dissertation study is an...
Show morePersonal wellness and burnout have been common themes in research studies regarding professional satisfaction and career success. Personality characteristics in relation to job and career success among professionals have also been included in countless studies. However, Bateman and Crant (1993) defined and began research on the specific construct of the proactive personality and how it related to personal achievement, satisfaction, and success among executives. This dissertation study is an extension of their research in that the relationship between proactive personality, job satisfaction and levels of burnout among Licensed Mental Health Counselors in Florida specifically, is being examined. While proactive personality positively and significantly related to job satisfaction and satisfaction with being a counselor among the participants in this study, it did not significantly relate to feelings of emotional exhaustion or feelings of depersonalization toward clients. When age, salary, years licensed, and proactive personality in relation to job satisfaction and levels of burnout were included in the statistical analysis, proactive personality and salary positively related to job satisfaction, personal accomplishment, and satisfaction with being a counselor, but the relationships were not significant. Hence, the results of this study provides useful information regarding proactive personality and how it relates to the overall job satisfaction, levels of burnout among clinicians, and to assist in the development of wellness programs, burnout prevention, and in the empowerment of mental health professionals in this demanding field.
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Date Issued
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2013
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PURL
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http://purl.flvc.org/fcla/dt/3361058
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Subject Headings
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Burnout (Psychology), Work, Psychological aspects, Job satisfaction, Medical personnel, Job stress, Counselors, Mental health
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Format
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Document (PDF)
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Title
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aB- crystallin/sHSP is required for mitochondrial function in human ocular tissue.
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Creator
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McGreal, Rebecca., Charles E. Schmidt College of Science, Department of Biological Sciences
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Abstract/Description
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The central premise of this dissertation is that the small heat shock protein (sHSP), (Sa(BB-crystallin is essential for lens and retinal pigmented epithelial (RPE) cell function and oxidative stress defense. To date, the mechanism by which it confers protection is not known. We hypothesize that these functions could occur through its ability to protect mitochondrial function in lens and RPE cells. To test this hypothesis, we examined the expression of (Sa(BB-crystallin/sHSP in lens and RPE...
Show moreThe central premise of this dissertation is that the small heat shock protein (sHSP), (Sa(BB-crystallin is essential for lens and retinal pigmented epithelial (RPE) cell function and oxidative stress defense. To date, the mechanism by which it confers protection is not known. We hypothesize that these functions could occur through its ability to protect mitochondrial function in lens and RPE cells. To test this hypothesis, we examined the expression of (Sa(BB-crystallin/sHSP in lens and RPE cells, we observed its localization in the cells, we examined translocation to the mitochondria in these cells upon oxidative stress treatment, we determined its ability to form complexes with and protect cytochrome c (cyt c) against damage, and we observed its ability to preserve mitochondrial function under oxidative stress conditions in lens and RPE cells. In addition to these studies, we examined the effect of mutations of (Sa(BB-crystallin/sHSP on its cellular localization and translocation patterns under oxidative stress, its in vivo and in vitro chaperone activity, and its ability to protect cyt c against oxidation. Our data demonstrated that (Sa(BB-crystallin/sHSP is expressed at high levels in the mitochondria of lens and RPE cells and specifically translocates to the mitochondria under oxidative stress conditions. We demonstrate that (Sa(BB-crystallin/sHSP complexes with cyt c and protects it against oxidative inactivation. Finally, we demonstrate that (Sa(BB-crystallin/sHSP directly protects mitochondria against oxidative inactivation in lens and RPE cells. Since oxidative stress is a key component of lens cataract formation and age-related macular degeneration (AMD), these data provide a new paradigm for understanding the etiology of these diseases.
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Date Issued
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2012
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PURL
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http://purl.flvc.org/FAU/3342242
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Subject Headings
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Mitochondrial pathology, Chemical mutagenesis, Oxidative stress, Prevention, Cellular signal transduction, Eye, Diseases, Etiology, Molecular chaperones
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Format
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Document (PDF)
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Title
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Protecting Synaptic Function From Acute Oxidative Stress: A Novel Role For Big K+ (BK) Channels And Resveratrol-Like Compounds.
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Creator
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Bollinger, Wesley L., Dawson-Scully, Ken, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
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Abstract/Description
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Oxidative stress causes neural damage and inhibits essential cellular processes, such as synaptic transmission. Despite this knowledge, currently available pharmaceutical agents cannot effectively protect neural cells from acute oxidative stress elicited by strokes, heart attacks, and traumatic brain injuries in a real life clinical setting. Our lab has developed an electrophysiology protocol to identify novel drugs that protect an essential cellular process (neurotransmission) from acute...
Show moreOxidative stress causes neural damage and inhibits essential cellular processes, such as synaptic transmission. Despite this knowledge, currently available pharmaceutical agents cannot effectively protect neural cells from acute oxidative stress elicited by strokes, heart attacks, and traumatic brain injuries in a real life clinical setting. Our lab has developed an electrophysiology protocol to identify novel drugs that protect an essential cellular process (neurotransmission) from acute oxidative stress-induced damage. Through this doctoral dissertation, we have identified three new drugs, including a Big K+ (BK) K+ channel blocker (iberiotoxin), resveratrol, and a custom made resveratrol-like compound (fly2) that protect synaptic function from oxidative stress-induced insults. Further developing these drugs as neuroprotective agents may prove transformative in protecting the human brain from acute oxidative stress elicited by strokes, heart attacks, and traumatic brain injuries. Inhibiting the protein kinase G (PKG) pathway protects neurotransmission from acute oxidative stress. This dissertation has expanded upon these findings by determining that the PKG pathway and BK K+ channels function through independent biochemical pathways to protect neurotransmission from acute oxidative stress. Taken together, this dissertation has identified two classes of compounds that protect neurotransmission from acute oxidative stress, including resveratrol-like compounds (resveratrol, fly2) and a BK K+ channel inhibitor (iberiotoxin). Further developing these drugs in clinical trials may finally lead to the development of an effective neuroprotective agent.
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Date Issued
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2018
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PURL
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http://purl.flvc.org/fau/fd/FA00013054
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Subject Headings
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Neural transmission., Oxidative stress., Large-Conductance Calcium-Activated Potassium Channels., Neuroprotective Agents.
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Format
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Document (PDF)
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Title
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THE EFFECTS OF MINDFULNESS TRAINING ON BDNF LEVELS, DEPRESSION, ANXIETY, AND STRESS LEVELS OF COLLEGE STUDENTS.
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Creator
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Bosso, Kathryn B., Brigman, Greg, Florida Atlantic University, Department of Counselor Education, College of Education
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Abstract/Description
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The purpose of this randomized control study was to examine the effects of the use of a mindfulness smartphone app on student self-reported levels of depression, anxiety, and stress, and serum levels of brain-derived neurotrophic factor (BDNF). The sample included college students enrolled in courses at a university in South Florida. Forty-four students were randomly allocated to either the mindfulness app group (n = 22) or the control group (n = 22). Participants in the mindfulness app group...
Show moreThe purpose of this randomized control study was to examine the effects of the use of a mindfulness smartphone app on student self-reported levels of depression, anxiety, and stress, and serum levels of brain-derived neurotrophic factor (BDNF). The sample included college students enrolled in courses at a university in South Florida. Forty-four students were randomly allocated to either the mindfulness app group (n = 22) or the control group (n = 22). Participants in the mindfulness app group were instructed to complete a guided meditation on the app for 10 minutes per day for 5 weeks. Participants in the control group were offered the intervention after the 5-week protocol ended. A pretest-posttest design was used to investigate the effects of the mindfulness app intervention on self-reported levels of depression, anxiety, and stress, in addition to serum level BDNF.
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Date Issued
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2020
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PURL
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http://purl.flvc.org/fau/fd/FA00013452
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Subject Headings
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College students, Mindfulness (Psychology), Brain-Derived Neurotrophic Factor, Depression, Anxiety, Stress
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Format
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Document (PDF)
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Title
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The finite element method as a parametric tool in the design and analysis of a pressure vessel having a threaded closure.
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Creator
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Merkl, Garrett Andrew., Florida Atlantic University, Case, Robert O., Tsai, Chi-Tay, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
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Abstract/Description
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The finite element method is a very powerful tool used to analyze a variety of problems in engineering. This thesis looks at the finite element method as a tool and several important modeling features of concern. A well known finite element software package, ANSYS, will be used to demonstrate a diverse number of its capabilities, and several procedures followed in solving a specific engineering problem. The subject matter involves a nonlinear contact analysis of a pressure vessel having a...
Show moreThe finite element method is a very powerful tool used to analyze a variety of problems in engineering. This thesis looks at the finite element method as a tool and several important modeling features of concern. A well known finite element software package, ANSYS, will be used to demonstrate a diverse number of its capabilities, and several procedures followed in solving a specific engineering problem. The subject matter involves a nonlinear contact analysis of a pressure vessel having a threaded closure. The choice of this application is prompted by an interest in better understanding how the finite element method is implemented in the design and analysis of different pressure vessel parameters. A parametric finite element analysis was performed. Load and stress distributions along the threaded region of the vessel were examined for parameters including number of threads, thread pitch, diameter ratio, closure plug length, and thread profile.
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Date Issued
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1996
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PURL
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http://purl.flvc.org/fcla/dt/15243
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Subject Headings
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Finite element method, Pressure vessels--Design and construction, Strains and stresses--Mathematical models
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Format
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Document (PDF)
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Title
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Stress intensity factors and weight functions for surface cracks using boundary/finite element alternating methods.
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Creator
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Mu, Ruijia., Florida Atlantic University, Reddy, Dronnadula V., College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
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Abstract/Description
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In the present investigation, the boundary/finite element alternating methods are used to evaluate the stress intensity factors and weight functions for surface crack problems. For two dimensional problems, Westergaard stress functions are used to find the analytical solutions for an infinite plate with an embedded crack, subjected to crack face tractions, and the boundary element method for the numerical solution. The stress intensity factors and weight functions for an arbitrary plate with...
Show moreIn the present investigation, the boundary/finite element alternating methods are used to evaluate the stress intensity factors and weight functions for surface crack problems. For two dimensional problems, Westergaard stress functions are used to find the analytical solutions for an infinite plate with an embedded crack, subjected to crack face tractions, and the boundary element method for the numerical solution. The stress intensity factors and weight functions for an arbitrary plate with an edge crack subjected to mixed mode loads are obtained by the alternating technique. For three dimensional problems, an elliptical coordinate system and the gravity potential functions are used to derive the three dimensional analytical solutions for an infinite solid with an embedded crack. The analytical solutions are derived for the cases of shear tractions and normal tractions, separately, by assuming that the tractions are symmetric about both the major and minor axes. Superposition gives the general solutions. The analytical solutions and the finite element method, in conjunction with alternating technique, are used to evaluate the stress intensity factors for a solid with a semi-elliptical surface crack, subjected to arbitrary loads. A general approach to evaluate the weight functions for a two dimensional plate with a three dimensional semi-elliptical surface crack is formulated. Numerical examples are evaluated using the formulation developed in the present investigation. The results show good agreement with those from classical solutions. The convergence characteristics of the alternating methods are also discussed. Finally, the formulation is applied to welded plate T-joints with edge/semi-elliptical surface cracks, subjected to three point bending, to obtain stress intensity factors.
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Date Issued
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1992
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PURL
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http://purl.flvc.org/fcla/dt/12319
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Subject Headings
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Fracture mechanics, Finite element method--Data processing, Materials--Fatigue, Strains and stresses
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Format
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Document (PDF)
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Title
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Stress protein expression and green turtle fibropapillomatosis.
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Creator
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McGarrity, Monica E., Florida Atlantic University, Milton, Sarah L.
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Abstract/Description
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Green turtle fibropapillomatosis (GTFP) is a highly debilitating disease that affects several species of marine turtles worldwide. This study evaluated stress protein expression in red blood cells (RBCs), skin and tumors of green turtles (Chelonia mydas) from a GTFP-prevalent site and a more pristine, GTFP-free site. Levels of expression of HSP72 were significantly higher in healthy turtles from the GTFP-prevalent site compared to healthy turtles from the GTFP-free site, suggesting that these...
Show moreGreen turtle fibropapillomatosis (GTFP) is a highly debilitating disease that affects several species of marine turtles worldwide. This study evaluated stress protein expression in red blood cells (RBCs), skin and tumors of green turtles (Chelonia mydas) from a GTFP-prevalent site and a more pristine, GTFP-free site. Levels of expression of HSP72 were significantly higher in healthy turtles from the GTFP-prevalent site compared to healthy turtles from the GTFP-free site, suggesting that these turtles are, indeed stressed. Levels of HSP72 expression were also significantly higher in the nucleated RBCs than in skin tissues of turtles from both sites. These results demonstrate the utility of RBC stress protein evaluation as a new, minimally invasive method of evaluating stress loads in marine turtles as a factor in the potentially multifactorial etiology of GTFP. In addition, HSP72 and GP96 were present and detectable in tumors of diseased turtles, and thus show potential for use in treatment of GTFP.
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Date Issued
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2005
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PURL
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http://purl.flvc.org/fcla/dt/13269
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Subject Headings
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Green turtle--Diseases, Green turtle--Immunology, Stress (Physiology), Environmental monitoring
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Format
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Document (PDF)
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Title
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Study of edge effects in laminated sandwich specimens.
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Creator
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Mankuzhy, Pradeep Prabhakaran., Florida Atlantic University, Merry, Stephanie L., College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
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Abstract/Description
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Different methods have been employed to calculate the interlaminar stresses and to study the edge effect in a laminated sandwich specimens under uniaxial tension. However, Finite Element Analysis and Force Balance Method produced stress values which disagreed in both magnitude and sign, a controversy which exists in the case of composite laminates also. Experimental methods, photoelastic coating method and strain gaging, were attempted to obtain the strain distribution on the top surface of a...
Show moreDifferent methods have been employed to calculate the interlaminar stresses and to study the edge effect in a laminated sandwich specimens under uniaxial tension. However, Finite Element Analysis and Force Balance Method produced stress values which disagreed in both magnitude and sign, a controversy which exists in the case of composite laminates also. Experimental methods, photoelastic coating method and strain gaging, were attempted to obtain the strain distribution on the top surface of a sandwich specimen in three point bending. However, these conventional methods failed to show the sharp strain gradient that exists near the free edge. The Force Balance Method was simplified for sandwich specimens by considering the face laminate as a homogeneous and orthotropic material with averaged properties. Simplified expressions were also obtained for calculating the boundary layer thickness. The boundary layer thickness was found to vary linearly with core thickness for the cases considered.
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Date Issued
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1989
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PURL
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http://purl.flvc.org/fcla/dt/14523
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Subject Headings
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Sandwich construction--Fatigue, Composite materials, Strains and stresses, Structural analysis (Engineering)
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Format
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Document (PDF)
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Title
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Stress Protein and Anti-Apoptotic Protein Expression in Green Turtle (Chelonia mydas) Fibropapillomatosis.
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Creator
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Deming, Alissa C., Milton, Sarah L., Florida Atlantic University
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Abstract/Description
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Fibropapillomatosis (FP) is a devastating pandemic characterized by benign cutaneous neoplasias that is afflicting marine turtles worldwide. This study evaluated the expression of HSP72, GRP96, and Bcl-2 in tumor and healthy biopsies. These proteins are found in high levels in some tumors and prevent apoptosis, allowing tumor cells to survive. HSP72 and GRP96 are also known to initiate an immune response and may contribute to the regression often observed in FP tumors. Results were found that...
Show moreFibropapillomatosis (FP) is a devastating pandemic characterized by benign cutaneous neoplasias that is afflicting marine turtles worldwide. This study evaluated the expression of HSP72, GRP96, and Bcl-2 in tumor and healthy biopsies. These proteins are found in high levels in some tumors and prevent apoptosis, allowing tumor cells to survive. HSP72 and GRP96 are also known to initiate an immune response and may contribute to the regression often observed in FP tumors. Results were found that showed all three protective proteins had significantly higher levels in actively growing tumor tissue compared to healthy tissue. Specifically, cauliflower-like tumors, thought to be actively growing, were found to have higher levels of HSP72 and GRP96 compared to healthy skin, whereas smooth tumors, thought to be regressing, did not. These results offer insight into the molecular mechanisms behind the development of FP tumors and open a number of avenues for future research.
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Date Issued
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2008
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PURL
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http://purl.flvc.org/fau/fd/FA00000740
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Subject Headings
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Stress (Physiology), Green turtle--Diseases, Environmental monitoring, Green turtle--Immunology
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Format
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Document (PDF)
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Title
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Thermal response in Florida bridges.
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Creator
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Sandepudi, Krishna Srinivasa., Florida Atlantic University, Arockiasamy, Madasamy, College of Engineering and Computer Science, Department of Ocean and Mechanical Engineering
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Abstract/Description
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Reinforced and prestressed concrete bridges are subjected to non-linear temperature variations leading to complex thermal stresses which vary continuously with time. Though these stresses are often comparable with those produced by live and dead loads, little guidance is given in bridge design codes on how these stresses are accurately computed. The objective of this project is to study the response of Florida bridges in the extreme thermal environment The project is divided into the...
Show moreReinforced and prestressed concrete bridges are subjected to non-linear temperature variations leading to complex thermal stresses which vary continuously with time. Though these stresses are often comparable with those produced by live and dead loads, little guidance is given in bridge design codes on how these stresses are accurately computed. The objective of this project is to study the response of Florida bridges in the extreme thermal environment The project is divided into the following four tasks i) Computer modeling of the bridge and estimation of the thermal response. ii) Field measurements of temperatures in typical bridges. iii) Comparison of observed and estimated data. iv) Suggestions and/or revisions to the existing thermal stress allowances in the code. A computer software FETAB was used to model and analyze the thermal response of several bridge cross sections. Two single cell box girder bridges, located at the 1-595 and US-441 interchange, Fort Lauderdale, were instrumented with thermocouples and vibrating wire strain gages. The predicted temperature variations were found to compare fairly well with those measured in the field. Though the analytical values vary a little from the actual field data, emphasis was given to gain insight into the problems associated with the thermal effects in concrete bridges. Suggestions were made for revision of existing design code provisions for improved design of bridges.
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Date Issued
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1991
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PURL
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http://purl.flvc.org/fcla/dt/14759
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Subject Headings
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Bridges, Concrete--Thermal properties, Bridges, Concrete--Effect of temperature on, Thermal stresses
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Format
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Document (PDF)
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Title
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Analysis of KED-mediated wound response to biotic stress and mechanical damage in tomato plants (Solanum lycopersicum).
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Creator
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Nifakos, Nicholas, Zhang, Xing-Hai, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
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Abstract/Description
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Lysine-rich KED was previously identified from wounded tobacco (Nicotiana tabacum) leaves before the alignment of protein sequences between NtKED (Nicotiana tabacum KED) and SlKED (Solanum lycopersicum KED) were discovered to display 55.1% identity. Using previously generated SlKED knockout plants by CRISPR/Cas9, we performed biological assays, to investigate the role of KED in wound response to biotic and abiotic stress. Previous studies implied that the KED gene functions as a role in the...
Show moreLysine-rich KED was previously identified from wounded tobacco (Nicotiana tabacum) leaves before the alignment of protein sequences between NtKED (Nicotiana tabacum KED) and SlKED (Solanum lycopersicum KED) were discovered to display 55.1% identity. Using previously generated SlKED knockout plants by CRISPR/Cas9, we performed biological assays, to investigate the role of KED in wound response to biotic and abiotic stress. Previous studies implied that the KED gene functions as a role in the wound-induced mechanism, as well as suggested that it may also function in the plant defense system against biotic stress and insect herbivory. The results from bioassays using tobacco hornworm (Manduca sexta) have proven inconclusive thus far. Expression of KED is induced not only by mechanical wounding but also by touching such as brushing the leaves, indicating that this gene is sensitive to subtle environmental signal and may be involved in defense response against abiotic stress. To further investigate the KED gene’s role in the plant defense system, biological assays using both specialist and generalist herbivores, transcription analysis using various phytohormone mutant plants, and Evans blue cellular damage assays were performed. Our findings imply that the KED gene does not seem to have a long-term effect on insect herbivory but may have a shortterm anti-feeding effect against insect herbivores. Results from the Evans blue membrane damage assay indicate the KED gene may provide some benefit to mechanically damaged plants in a short-term period post-wounding of leaf tissues. Using the SlKED knockout as genetic tool, we conclude that this gene does not confer resistance to insect herbivores over a long-term but seems to provide a beneficial defense response in the short-term. Our membrane damage assay results also imply that this gene may be involved in membrane stabilization and repair of cellular damage after mechanical wounding.
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Date Issued
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2022
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PURL
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http://purl.flvc.org/fau/fd/FA00014001
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Subject Headings
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Plant gene expression, Solanum lycopersicum, Tomatoes--Effect of stress on
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Format
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Document (PDF)
Pages