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Title: Reciprocal regulation between taurine and glutamate response via Ca2+ - dependent pathways in retinal third-order neurons.
Creator: Bulley, Simon, Shen, Wen
Date Issued: 2010-08-24
PURL: http://purl.flvc.org/fcla/dt/3327274
Subject Headings: Amacrine Cells*/cytology, Amacrine Cells*/drug effects, Amacrine Cells*/metabolism, Ambystoma, Calcium/metabolism, Calcium Channels/metabolism, Cells, Cultured, Enzyme Inhibitors/metabolism, Excitatory Amino Acid Agonists/pharmacology, GABA Antagonists/pharmacology, Glutamic Acid/metabolism, Glycine Agents/pharmacology, Kainic Acid/pharmacology, Membrane Glycoproteins, Membrane Potentials, Neurotransmitter Agents, Retinal Ganglion Cells, Signal Transduction, Synaptic Transmission
Format: Document (PDF)

Title: Effects of stressors on differential gene expression and secondary metabolites by Axinella corrugata.
Creator: Grima, Jennifer., Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Sponges are an important source of bioactive marine natural products, or secondary metabolites. The common Caribbean reef sponge, Axinella corrugata, produces an antitumor and antibacterial chemical, stevensine. This study determined whether environmental stressors, such as elevated temperature and exposure to Amphibalanus amphitrite larvae, affect the production of stevensine by A.corrugata and if the stressors caused A.corrugata to exhibit differential gene expression. Temperature stress...
Show moreSponges are an important source of bioactive marine natural products, or secondary metabolites. The common Caribbean reef sponge, Axinella corrugata, produces an antitumor and antibacterial chemical, stevensine. This study determined whether environmental stressors, such as elevated temperature and exposure to Amphibalanus amphitrite larvae, affect the production of stevensine by A.corrugata and if the stressors caused A.corrugata to exhibit differential gene expression. Temperature stress resulted in no significant change in the production of stevensine; only two genes were significantly differentially expressed, including hsp70. Larval stressed resulted in increased production of stevensine and significant differential gene expression (more than seventy genes). This study suggests that A.corrugata may be resilient to elevations in temperature and that one of stevensine's roles in nature is as an antifoulant.
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Date Issued: 2013
PURL: http://purl.flvc.org/fcla/dt/3360781
Subject Headings: Axinellida, Sponges, Marine pharmacology, Adaptation (Biology), Gene expression, Genetic regulation, Stress (Physiology), Ecophysiology
Format: Document (PDF)

Title: Isolation and Structural Elucidation of Novel Bioactive Natural Products from Marine Organisms of the Western Atlantic Ocean.
Creator: Zhang, Long, West, Lyndon, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
Abstract/Description: The aim of this dissertation was to elaborate the exploration of biologically active secondary metabolites from the marine sponge Cacospongia cf. linteiformis collected from the Bahamas and the soft coral Briareum asbestinum collected from two different sites in Florida State, Boca Raton and Dry Tortugas. In chapter one, a review on previous chemical and biological studies of the marine sponge C. cf. linteiformis and soft coral B. asbestinum is provided. Particular attention is given to...
Show moreThe aim of this dissertation was to elaborate the exploration of biologically active secondary metabolites from the marine sponge Cacospongia cf. linteiformis collected from the Bahamas and the soft coral Briareum asbestinum collected from two different sites in Florida State, Boca Raton and Dry Tortugas. In chapter one, a review on previous chemical and biological studies of the marine sponge C. cf. linteiformis and soft coral B. asbestinum is provided. Particular attention is given to spongianolides and briarellins, two important classes of compounds isolated from C. cf. linteiformis and B. asbestinum, respectively, and their structural features and diverse bioactivities. In chapter two, the isolation and relative configuration determination of four epimeric sesterterpenoids, spongianolides E & F (18c, 18d, 19c, 19d) from C. cf. linteiformis collected from the Bahamas are discussed. Thanks to chemical modification (acetylation), diastereomeric 18c&18d and 19c&19d, respectively, were able to be isolated using chromatographic techniques for the first time, and then the relative configurations of 18c, 18d, 19c, 19d were determined based on NOESY NMR experiments. The bioactivity of mixture of compounds 18c, 18d, 19c, 19d were tested and it exhibited inhibition against Schnurri-3 (a regulator of postnatal bone mass). In chapter three, the isolation and structural elucidation of four new compounds, florellins A-D (49-52), from B. asbestinum collected off the coast of Boca Raton, FL are discussed. The molecular structures of these compounds were established by spectroscopic analysis. Compounds 49-52 are the first briarellins containing an acyl group at C-13, while 49 and 50 are the first briarellins possessing acylation at C-15. Florellins A–C (49-51) were screened and found cytotoxic against three human cell lines, BT474, WM266−4 and HEK293. In chapter four, the isolation and structural elucidation of four new compounds, florellins E-H (57-60), from B. asbestinum collected in Dry Tortugas, FL are discussed. The molecular structures of these compounds were established by spectroscopic analysis. Florellins F (58) and H (60) were screened against three human cell lines, BT474, WM266−4 and HEK293, but no cytotoxicity was exhibited. In chapter five, all the experimental procedures are described, including analytical instruments, animal materials, extraction and isolation processes, spectroscopic data and protocols of bioassays.
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Date Issued: 2017
PURL: http://purl.flvc.org/fau/fd/FA00004850, http://purl.flvc.org/fau/fd/FA00004850
Subject Headings: Pharmacognosy., Natural products--Analysis., Marine pharmacology., Marine biotechnology., Marine algae--Biotechnology., Bioactive compounds.
Format: Document (PDF)

Title: Origin and mechanism of terpene biosynthesis in Pseudopterogorgia spp.
Creator: Boehnlein, Jennifer M., Florida Atlantic University, Kerr, Russell G.
Abstract/Description: The goal of this work was to investigate the biosynthetic origins of diterpene natural products (pseudopterosins, kallolides, bipinnatins, and cembrenes) from corals of the genus Pseudopterogorgia as well as the biosynthetic pathways by which they are produced. These studies have shown that the pseudopterosins from Pseudopterogorgia elisabethae are biosynthesized within the algal symbiont (or possibly a bacterium or fungus associated with the symbiont), are not inducible by manipulation of...
Show moreThe goal of this work was to investigate the biosynthetic origins of diterpene natural products (pseudopterosins, kallolides, bipinnatins, and cembrenes) from corals of the genus Pseudopterogorgia as well as the biosynthetic pathways by which they are produced. These studies have shown that the pseudopterosins from Pseudopterogorgia elisabethae are biosynthesized within the algal symbiont (or possibly a bacterium or fungus associated with the symbiont), are not inducible by manipulation of light levels, and do not change as a result of transplantation to new locations. Studies on Pseudopterogorgia bipinnata revealed that only one chemotype is capable of biosynthesizing the kallolide family of diterpenes. The biosynthetic pathway which gives rise to the kallolides has been shown to involve members of another family of diterpenes, the bipinnatins, which coexist within the coral holobiont. Two diterpene cyclase products have been discovered within P. bipinnata chemotype A, cembrene and neocembrene, and it has been shown that neocembrene gives rise to the kallolides. Finally, the enzymatic conversion of bipinnatin J to kallolide A has shown for the first time that these compounds are in fact biogenetically related.
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Date Issued: 2006
PURL: http://purl.flvc.org/fcla/dt/12205
Subject Headings: Terpenes--Synthesis, Marine pharmacology, Alcyonacea, Biosynthesis, Anti-inflammatory agents
Format: Document (PDF)

Title: Shifts in copepod grazing on varying concentrations of two bioluminescent dinoflagellate species.
Creator: Breitlow, Karen A., Florida Atlantic University, Widder, Edith A.
Abstract/Description: Grazing on bioluminescent and nonluminescent prey by the copepod Acartia tonsa was examined to determine the effect dinoflagellate bioluminescence has on copepod feeding preferences and rates. The percentage of cells ml-1 remaining after 30 minutes of copepod grazing on the dinoflagellates Lingulodinium polyedrum and Pyrodinium bahamense in their bioluminescent and nonbioluminescent phases was used to compare results for concentrations of 10, 1,000, and 3,000 cells ml-1. The nonluminescent...
Show moreGrazing on bioluminescent and nonluminescent prey by the copepod Acartia tonsa was examined to determine the effect dinoflagellate bioluminescence has on copepod feeding preferences and rates. The percentage of cells ml-1 remaining after 30 minutes of copepod grazing on the dinoflagellates Lingulodinium polyedrum and Pyrodinium bahamense in their bioluminescent and nonbioluminescent phases was used to compare results for concentrations of 10, 1,000, and 3,000 cells ml-1. The nonluminescent diatom Thalassiosira eccentrica was later offered along with each of the dinoflagellates. When diatoms were offered with P. bahamense, the copepods consumed them in equal amounts regardless of bioluminescence at the lower concentration, while the higher concentration showed decreased grazing with bioluminescence. Nonbioluminescent L. polyedrum was consumed at a high rate for all concentrations, but grazing decreased once they were bioluminescent. Copepods switched to T. eccentrica once the dinoflagellates became bioluminescent, except at 3,000 cells ml-1, at which all grazing was reduced. These results indicate there may be a threshold concentration separating two functions of dinoflagellate bioluminescence.
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Date Issued: 2006
PURL: http://purl.flvc.org/fcla/dt/13357
Subject Headings: Dinoflagellates, Marine pharmacology, Bioluminescence, Marine animals--Behavior, Predation (Biology), Adaptation (Biology)
Format: Document (PDF)

Title: Isolation and biosynthesis of terpenes in Pseudopterogorgia elisabethae and Leptogorgia minimata.
Creator: von Saalfeld, Silke., Florida Atlantic University, Kerr, Russell G.
Abstract/Description: The marine environment has proven to be an extremely rich source of novel natural products with activities in a variety of biological assays. The field of marine natural products chemistry has enjoyed an explosion of growth over the past 40 years and currently there are marine-derived metabolites in clinical trials for cancer, analgesia, allergy and cognitive diseases. Even with this tremendous development, it is clear that chemists and pharmacologists are only beginning to scratch the...
Show moreThe marine environment has proven to be an extremely rich source of novel natural products with activities in a variety of biological assays. The field of marine natural products chemistry has enjoyed an explosion of growth over the past 40 years and currently there are marine-derived metabolites in clinical trials for cancer, analgesia, allergy and cognitive diseases. Even with this tremendous development, it is clear that chemists and pharmacologists are only beginning to scratch the surface of the biomedical potential of marine organisms. One problem with this source of new pharmaceuticals is that the supply of such compounds can be problematic. The thesis describes projects directed at fundamental biosynthetic questions regarding terpenes from marine corals. In a general sense, these projects are directed as addressing the supply issue identified above. One project examined the origin of terpene building blocks in the coral Pseudopterogorgia elisabethae. A second project focused on the characterization of terpenes from Leptogorgia minimata. Here, a new cembranoid diterpene was isolated. Experiments were also conducted to identify a microbial source of terpene biosynthesis in this system. Data strongly supported a bacterial origin of these compounds.
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Date Issued: 2006
PURL: http://purl.flvc.org/fcla/dt/13325
Subject Headings: Natural products--Synthesis, Marine pharmacology, Aquaculture, Terpenes, Marine chemical ecology
Format: Document (PDF)

Title: Isolation, Analysis and Origin of Bioactive Diterpenes in Pseudopterogorgia acerosa.
Creator: Kate, Abhijeet S., Florida Atlantic University, Kerr, Russell G., Charles E. Schmidt College of Science, Department of Chemistry and Biochemistry
Abstract/Description: Nature has served human kind m many ways, one of which is a source of medicines. Natural products from marine sources represent a relatively new area of research and have shown tremendous potential as a source of new chemical entities in drug discovery. Caribbean gorgomans corals of the genus Pseudopterogorgia have been shown to produce a variety of chemically interesting and biologically significant secondary metabolites. ln this dissertation, the Caribbean coral Pseudopterogorgia acerosa...
Show moreNature has served human kind m many ways, one of which is a source of medicines. Natural products from marine sources represent a relatively new area of research and have shown tremendous potential as a source of new chemical entities in drug discovery. Caribbean gorgomans corals of the genus Pseudopterogorgia have been shown to produce a variety of chemically interesting and biologically significant secondary metabolites. ln this dissertation, the Caribbean coral Pseudopterogorgia acerosa has been investigated for the presence of novel diterpenes and these compounds were found to belong to three different classes: pseudopteranoids, bis-pseudopteranoids and lipidyl pseudopteranes. Nine of these were new compounds. The structural elucidation of these compounds was performed using spectroscopic means such as l D and 20 NMR, and mass spectroscopy. There is growing evidence that secondary metabolites isolated from manne invertebrates may actually be produced by a bacterial symbiont. The research studies in our laboratory regarding the source of diterpenes in the selected gorgonian corals suggested a bacterial origin. The hypothesis that coral associated bacteria are the source of diterpenes in the coral P. acerosa, was evaluated using the series of experiments and evidence supported this biosynthetic origin. A study comparing the "gall" tissue and healthy coral tissue in terms of diterpene content and culturable bacterial communities showed that different groups of diterpenes were concentrated in different coral tissue types. It was also observed that the bacterial populations associated with the "gall" and healthy tissues were considerably different. Furthermore, observed specificity in antimicrobial activity of certain groups of compounds against bacteria isolated from the same coral suggested the ecological role of these compounds. This work with "gall" tissue supports the hypothesis that diseased coral tissue represents an excellent source of bioactive natural products for drug discovery. Additionaly, a simple LC-MS method was developed for the analysis of anticancer drug carmustine in plasma.
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Date Issued: 2008
PURL: http://purl.flvc.org/fau/fd/FA00000864
Subject Headings: Marine pharmacology, Coral reef ecology--Caribbean Area, Natural products--Synthesis
Format: Document (PDF)

Title: Long-term glycine propionyl-l-carnitine supplementation and paradoxical effects on repeated anaerobic sprint performance.
Creator: Jacobs, Patrick L., Goldstein, Erica R.
Date Issued: 2010-10-28
PURL: http://purl.flvc.org/fcla/dt/3327167
Subject Headings: Anaerobic Threshold --Drug Effects, Anaerobic Threshold --Physiology, Athletic Performance, Athletic Performance --Physiology, Carnitine --Pharmacology, Dietary Supplementation, Exercise Physiology, Exercise Physiology --Drug Effects, Exercise Tolerance --Drug Effects, Glycine --Administration & Dosage, Glycine --Pharmacology, Nitric Oxide --Metabolism, Nitric Oxide --Pharmacodynamics
Format: Document (PDF)

Title: Glycine propionyl-L-carnitine produces enhanced anaerobic work capacity with reduced lactate accumulation in resistance trained males.
Creator: Jacobs, Patrick L., Goldstein, Erica R., Blackburn, Will, Orem, Ihsan, Hughes, John J.
Date Issued: 2009-04-02
PURL: http://purl.flvc.org/fcla/dt/3327165
Subject Headings: Exercise Physiology, Glycine --Pharmacodynamics, Nitric Oxide --Metabolism, Carnitine --Pharmacology, Physical Endurance, Athletic Performance, Dietary Supplementation, Exercise Physiology --Drug Effects, Resistence Training
Format: Document (PDF)

Title: Taurine protection of PC12 cells against endoplasmic reticulum stress induced by oxidative stress.
Creator: Pan, Chunliu, Giraldo, Grace S., Prentice, Howard, Wu, Jang-Yen
Date Issued: 2010-08-24
PURL: http://purl.flvc.org/fcla/dt/3327276
Subject Headings: Oxidative Stress, Oxidative Stress --drug effects, Oxidative Stress --physiology, Antioxidants --pharmacology, Apoptosis Regulatory Proteins, Proto-Oncogene Proteins c-bcl-2, PC12 Cells --drug effects, Endoplasmic Reticulum --drug effects, Transcription Factor CHOP, Taurine
Format: Document (PDF)

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