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- Title
- The Comparison of High-Intensity Interval Exercise vs. Continuous Moderate Exercise on C1q/TNF-Related Protein-9 Expression and Flow-Mediated Vasodilation.
- Creator
- Fico, Brandon G., Huang, Chun-Jung, Florida Atlantic University, College of Education, Department of Exercise Science and Health Promotion
- Abstract/Description
-
The primary purpose of this study was to investigate the effect of acute high-intensity interval exercise (HIIE) vs. continuous moderate-intensity exercise (CME) on serum CTRP9 and brachial FMD responses in obese and normal-weight subjects. Sixteen participants (9 obese and 7 normal-weight) completed HIIE and CME in a randomized fashion. Our results showed a significant time effect for CTRP9 immediately following acute HIIE and CME in both groups. Furthermore, both significant treatment by...
Show moreThe primary purpose of this study was to investigate the effect of acute high-intensity interval exercise (HIIE) vs. continuous moderate-intensity exercise (CME) on serum CTRP9 and brachial FMD responses in obese and normal-weight subjects. Sixteen participants (9 obese and 7 normal-weight) completed HIIE and CME in a randomized fashion. Our results showed a significant time effect for CTRP9 immediately following acute HIIE and CME in both groups. Furthermore, both significant treatment by time and group by time interactions for FMD were observed following both exercise protocols, with greater CME-induced FMD response in obese subjects than normal-weight subjects. Additionally, a positive correlation in percent change (baseline to peak) between CTRP9 and FMD was observed following acute CME. These findings support acute CME for improvement of endothelial function in obesity. Furthermore, the novel results from this study provide a foundation for additional examination of the mechanisms of exercise-mediated CTRP9 on endothelial function.
Show less - Date Issued
- 2017
- PURL
- http://purl.flvc.org/fau/fd/FA00004915, http://purl.flvc.org/fau/fd/FA00004915
- Subject Headings
- Cardiovascular system--Physiology., Biological transport., Exercise--Physiological aspects., Interval training--Physiological aspects., Bioinformatics., Exercise--Health aspects., Lifestyles--Health aspects., Gene expression.
- Format
- Document (PDF)
- Title
- The expression and analysis of a lysine-rich wound-response protein in tomato plants.
- Creator
- Kaplan, Noah, Zhang, Xing-Hai, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
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Understanding the genetic regulation of the response to wounding and wound healing in fruiting plants is imperative to maintaining agricultural sustainability, preserving the quality of food supplies, and ensuring the economic viability of agriculture. Many genes are known to be induced by wounding, providing both structural repair and defense. The KED gene in tobacco (Nicotiana tabacum) has been shown to be induced by wounding. We have identified its homologue gene in tomato (Solanum...
Show moreUnderstanding the genetic regulation of the response to wounding and wound healing in fruiting plants is imperative to maintaining agricultural sustainability, preserving the quality of food supplies, and ensuring the economic viability of agriculture. Many genes are known to be induced by wounding, providing both structural repair and defense. The KED gene in tobacco (Nicotiana tabacum) has been shown to be induced by wounding. We have identified its homologue gene in tomato (Solanum lycopersicum) that we named SlKED. We have analyzed gene expression pattern of SlKED through tomato growth and development and in response to wounding as well as hormonal and inhibitor treatments. We found that the plant hormone ethylene played a major role in the expression of SlKED. To further identify evidence for physiological and transductional functions of KED and SlKED, the tobacco KED gene was introduced to tomato and overexpressed by the fruit tissue-active PUN1 promoter from pepper (Capsicum annuum,). The expression of this gene was compared to the expression of the native SlKED gene and other known wound response genes in both the wild-type and transgenic tomato plants. The upregulation of the native SlKED gene by wounding was significantly muted in the tobacco KED-expressing transgenic plants. The expression of other genes known to be associated with wound response transduction pathways was also altered. Our studies implicate the KED gene in defense mechanisms for mechanical stress in tomato plants.
Show less - Date Issued
- 2016
- PURL
- http://purl.flvc.org/fau/fd/FA00004773, http://purl.flvc.org/fau/fd/FA00004773
- Subject Headings
- Wound healing., Wounds and injuries--Genetic aspects., Plant gene expression., Plant genetic regulation., Nanostructured materials--Physiological effect., Biomedical engineering.
- Format
- Document (PDF)
- Title
- Population Structure and Gene Expression of the Coral Montastraea cavernosa in the Northern Florida Reef Tract.
- Creator
- Dodge, Danielle, Voss, Joshua, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
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Coral reefs on Florida’s Reef Tract (FRT) are susceptible to many anthropogenic influences including controlled freshwater discharges and agricultural runoff as well as high natural environmental variability from seasonal rainfall, runoff and upwelling. To better understand coral population structure and responses to sublethal stressors, populations of the scleractinian coral Montastraea cavernosa in the northern FRT were examined using a combination of genomic and transcriptomic techniques....
Show moreCoral reefs on Florida’s Reef Tract (FRT) are susceptible to many anthropogenic influences including controlled freshwater discharges and agricultural runoff as well as high natural environmental variability from seasonal rainfall, runoff and upwelling. To better understand coral population structure and responses to sublethal stressors, populations of the scleractinian coral Montastraea cavernosa in the northern FRT were examined using a combination of genomic and transcriptomic techniques. Microsatellite genetic markers identified high local retention among sites and a slight southward gene flow. An in-situ temporal gene expression analysis utilizing a tag-based sequencing transcriptomic approach was used to analyze baseline coral health at St. Lucie Reef (SLR), off Stuart, FL. Temporal variation had the greatest influence of differential gene expression among M. cavernosa at SLR. Results will be shared with local resource managers and coupled with a complementary ex-situ experimental trial.
Show less - Date Issued
- 2017
- PURL
- http://purl.flvc.org/fau/fd/FA00005920
- Subject Headings
- Dissertations, Academic -- Florida Atlantic University, Montastraea, Coral reef ecology--Florida., Corals--Effect of stress on, Gene expression--Analysis.
- Format
- Document (PDF)
- Title
- Molecular pathway identification using microarray technology.
- Creator
- Tress, Matthew David., Florida Atlantic University, Narayanan, Ramaswamy
- Abstract/Description
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Harnessing the human genome using bioinformatics lead to the discovery of a highly cancer-selective gene, Single Minded 2 gene (SIM2). An isoform of the SIM2 gene, the short-form (SIM2-s), was shown to be specific to colon, pancreas, and prostate tumors. Antisense inhibition of SIM2-s in a colon carcinoma derived cell line (RKO) caused inhibition of gene expression, growth inhibition and apoptosis in vitro and in nude mice tumorigenicity models. To understand the mechanism of Sim2-s antisense...
Show moreHarnessing the human genome using bioinformatics lead to the discovery of a highly cancer-selective gene, Single Minded 2 gene (SIM2). An isoform of the SIM2 gene, the short-form (SIM2-s), was shown to be specific to colon, pancreas, and prostate tumors. Antisense inhibition of SIM2-s in a colon carcinoma derived cell line (RKO) caused inhibition of gene expression, growth inhibition and apoptosis in vitro and in nude mice tumorigenicity models. To understand the mechanism of Sim2-s antisense, the antisense treated RKO colon cancer cells were monitored for genome wide expression using Affymetrix GeneChipRTM technology. A list of apoptosis related genes was generated using GeneSpringRTM software. Select GeneChip RTM output was validated by Quantitative RT-PCR. Relevance of a key gene, Growth arrest and DNA damage inducible (GADD45a), in the SIM2-s pathway was established. These results will provide a basis for the future experiments to understand the mechanism underlying Sim2-s activation in specific tumors.
Show less - Date Issued
- 2004
- PURL
- http://purl.flvc.org/fcla/dt/13146
- Subject Headings
- Medical informatics, DNA microarrays--Diagnostic use, Cancer--Genetic aspects, Apoptosis--Molecular aspects, Human genetics--Variation, Gene expression--Research--Methodology
- Format
- Document (PDF)
- Title
- Myofibril-Inducing RNA (MIR) is essential for tropomyosin expression and myofibrillogenesis in axolotl hearts.
- Creator
- Zhang, Chi, Jia, Pingping, Huang, Xupei, Sferrazza, Gian Franco, Athauda, Gagani, Achary, Mohan P., Wang, Jikui, Lemanski, Sharon L., Lemanski, Larry F.
- Date Issued
- 2009-09-03
- PURL
- http://purl.flvc.org/fcla/dt/3327272
- Subject Headings
- Ambystoma mexicanum --embryology, Ambystoma mexicanum --genetics, Base Sequence, DNA, Complementary --genetics, Endoderm --physiology, Exons --genetics, Gene Expression Regulation, Developmental, Gene Knockdown Techniques, Genes, Recessive, Heart --embryology, Models, Genetic, Molecular Sequence Data, Muscle Proteins --biosynthesis, Muscle Proteins --genetics, Myocardial Contraction --physiology, Protein Isoforms, Reverse Transcriptase Polymerase Chain Reaction, Tropomyosin
- Format
- Document (PDF)
- Title
- Analysis of the Role of Autophagy in Dauer Formation and Dauer Recovery Regulated by TGF-β Signaling Pathway in Caenorhabditis elegans.
- Creator
- Ritter, Portia, Jia, Kailiang, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
-
Caenorhabditis elegans optionally enter into a dauer diapause phase that results in a prolonged life in a semi-dormant state. Entry into and recovery from dauer diapause includes many physical changes in body structure, physiology, and gene expression. Entry into dauer diapause is regulated by several signaling pathways including transforming growth factor (TGF-β). Autophagy plays an important role in dauer formation and recover. During dauer transformation autophagy is up-regulated and may...
Show moreCaenorhabditis elegans optionally enter into a dauer diapause phase that results in a prolonged life in a semi-dormant state. Entry into and recovery from dauer diapause includes many physical changes in body structure, physiology, and gene expression. Entry into dauer diapause is regulated by several signaling pathways including transforming growth factor (TGF-β). Autophagy plays an important role in dauer formation and recover. During dauer transformation autophagy is up-regulated and may play a role in remodeling the molecular structure for long term survival during dauer diapause. This research helps determine the role of autophagy in dauer development and recovery mediated through the TGF-β signaling pathway. This research also determines in which tissue autophagy is necessary for dauer formation and recovery through TGF-β signaling. This research is shedding light on the function of autophagy in the TGF-β signaling pathway, both processes of which have been linked to tumorigenesis, heart disease and cancer.
Show less - Date Issued
- 2017
- PURL
- http://purl.flvc.org/fau/fd/FA00004921, http://purl.flvc.org/fau/fd/FA00004921
- Subject Headings
- Aging--Molecular aspects., Aging--Physiological aspects., Caenorhabditis elegans--Molecular genetics., Autophagic vacuoles., Gene expression., Apoptosis., Cellular signal transduction., DNA-binding proteins., Transforming growth factors-beta--Receptors.
- Format
- Document (PDF)
