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A Kinetic Aggregation Assay Enabling Selective and Sensitive Aβ Amyloid Quantification in Cells and Tissues
Title
A Kinetic Aggregation Assay Enabling Selective and Sensitive Aβ Amyloid Quantification in Cells and Tissues.
Creator
Deguo Du, Amber N. Murray, Ehud Cohen, Hyun-Eui Kim, Ryan Simkovsky, Andrew Dillin, Jeffery W. Kelly
Abstract/Description
The process of amyloid-β (Aβ) fibril formation is genetically and pathologically linked to Alzheimer's disease (AD). Thus, a selective and sensitive method for the quantification of Aβ amyloid fibrils in complex biological samples enables a variety of hypotheses to be tested. Herein we report the basis for a quantitative in vitro kinetic aggregation assay that detects seeding competent Aβ aggregates in mammalian cell culture media, in Caenorhabditis elegans lysate and in mouse brain...
Show moreThe process of amyloid-β (Aβ) fibril formation is genetically and pathologically linked to Alzheimer's disease (AD). Thus, a selective and sensitive method for the quantification of Aβ amyloid fibrils in complex biological samples enables a variety of hypotheses to be tested. Herein we report the basis for a quantitative in vitro kinetic aggregation assay that detects seeding competent Aβ aggregates in mammalian cell culture media, in Caenorhabditis elegans lysate and in mouse brain homogenate. Sonicated, proteinase K treated Aβ-fibril-containing tissue homogenates or cell culture media were added to an initially monomeric Aβ1–40 reporter peptide to seed an in vitro nucleated aggregation reaction. The reduction in the half time (t50) of the amyloid growth phase is proportional to the quantity of seeding-competent Aβ aggregates present in the biological sample. An ion exchange resin amyloid isolation strategy from complex biological samples is demonstrated as an alternative to improve the sensitivity and linearity of the kinetic aggregation assay.
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Date Issued
2011
PURL
http://purl.flvc.org/fau/fd/FAUIR000498
Format
Document (PDF)
Biomass offsets little or none of permafrost carbon release from soils, streams, and wildfire: an expert assessment
Title
Biomass offsets little or none of permafrost carbon release from soils, streams, and wildfire: an expert assessment.
Creator
Abbott, Benjamin W., Jones, Jeremy B., Schuur, Edward A. G., Chapin III, F. Stuart, Bowden, William B., Bret-Harte, M. Syndonia, Epstein, Howard E., Flannigan, Michael D., Harms, Tamara K., Hollingsworth, Teresa N., Mack, Michelle C., McGuire, A. David, Natali, Susan M., Rocha, Adrian V., Tank, Suzanne E., Turetsky, Merritt R., Vonk, Jorien E., Wickland, Kimberly P., Aiken, George R., Alexander, Heather D., Amon, Rainer M. W., Benscoter, Brian, Bergeron, Yves, Bishop, Kevin, Blarquez, Olivier, Bond-Lamberty, Ben, Breen, Amy L., Buffam, Ishi, Cai, Yihua, Carcaillet, Christopher, Carey, Sean K., Chen, Jing M., Chen, Han Y. H., Christensen, Torben R., Cooper, Lee W., Cornelissen, J. Hans C., de Groot, William J., DeLuca, Thomas H., Dorrepaal, Ellen, Fetcher, Ned, Finlay, Jacques C., Forbes, Bruce C., French, Nancy H. F., Gauthier, Sylvie, Girardin, Martin P., Goetz, Scott J., Goldammer, Johann G., Gough, Laura, Grogan, Paul, Guo, Laodong, Higuera, Philip E., Hinzman, Larry, Hu, Feng Sheng, Hugelius, Gustaf, Jafarov, Elchin E., Jandt, Randi, Johnstone, Jill F., Jan Karlsson, Kasischke, Eric S., Kattner, Gerhard, Kelly, Ryan, Keuper, Frida, Kling, George W., Kortelainen, Pirkko, Kouki, Jari, Kuhry, Peter, Laudon, Hjalmar, Laurion, Isabelle, Macdonald, Robie W., Mann, Paul J., Martikainen, Pertti J., McClelland, James W., Molau, Ulf, Oberbauer, Steven F., Olefeldt, David, Paré, David, Parisien, Marc-André, Payette, Serge, Peng, Changhui, Pokrovsky, Oleg S., Rastetter, Edward B., Raymond, Peter A., Raynolds, Martha K., Rein, Guillermo, Reynolds, James F., Robards, Martin, Rogers, Brendan M., Schädel, Christina, Schaefer, Kevin, Schmidt, Inger K., Shvidenko, Anatoly, Sky, Jasper, Spencer, Robert G. M., Starr, Gregory, Striegl, Robert G., Teisserenc, Roman, Tranvik, Lars J., Virtanen, Tarmo, Welker, Jeffrey M., Zimov, Sergei
Date Issued
2016-03-07
PURL
http://purl.flvc.org/fau/fd/FAUIR000121
Format
Citation
Biomass offsets little or none of permafrost carbon release from soils, streams, and wildfire: an expert assessment
Title
Biomass offsets little or none of permafrost carbon release from soils, streams, and wildfire: an expert assessment.
Creator
Abbott, Benjamin W, Jones, Jeremy B, Schuur, Edward A G, Chapin III, F Stuart, Bowden, William B, Bret-Harte, M Syndonia, Epstein, Howard E, Flannigan, Michael D, Harms, Tamara K, Hollingsworth, Teresa N, Mack, Michelle C, McGuire, A David, Natali, Susan M, Rocha, Adrian V, Tank, Suzanne E, Turetsky, Merritt R, Vonk, Jorien E, Wickland, Kimberly P, Aiken, George R, Alexander, Heather D, Amon, Rainer M W, Benscoter, Brian W, Bergeron, Yves, Bishop, Kevin, Blarquez, Olivier, Ben Bond-Lamberty, Breen, Amy L, Buffam, Ishi, Cai, Yihua, Carcaillet, Christopher, Carey, Sean K, Chen, Jing M, Chen, Han Y H, Christensen, Torben R, Cooper, Lee W, Cornelissen, J Hans C, de Groot, William J, DeLuca, Thomas H, Dorrepaal, Ellen, Fetcher, Ned, Finlay, Jacques C, Forbes, Bruce C, French, Nancy H F, Gauthier, Sylvie, Girardin, Martin P, Goetz, Scott J, Goldammer, Johann G, Gough, Laura, Grogan, Paul, Guo, Laodong, Higuera, Philip E, Hinzman, Larry, Hu, Feng Sheng, Hugelius, Gustaf, Jafarov, Elchin E, Jandt, Randi, Johnstone, Jill F, Jan Karlsson, Kasischke, Eric S, Kattner, Gerhard, Kelly, Ryan, Keuper, Frida, Kling, George W, Kortelainen, Pirkko, Kouki, Jari, Kuhry, Peter, Laudon, Hjalmar, Laurion, Isabelle, Macdonald, Robie W, Mann, Paul J, Martikainen, Pertti J, McClelland, James W, Molau, Ulf, Oberbauer, Steven F, Olefeldt, David, Paré, David, Parisien, Marc-André, Payette, Serge, Peng, Changhui, Pokrovsky, Oleg S, Rastetter, Edward B, Raymond, Peter A, Raynolds, Martha K, Rein, Guillermo, Reynolds, James F, Robards, Martin, Rogers, Brendan M, Schädel, Christina, Schaefer, Kevin, Schmidt, Inger K, Shvidenko, Anatoly, Sky, Jasper, Spencer, Robert G M, Starr, Gregory, Striegl, Robert G, Teisserenc, Roman, Tranvik, Lars J, Virtanen, Tarmo, Welker, Jeffrey M, Zimov, Sergei
Date Issued
2016-03-07
PURL
http://purl.flvc.org/fau/flvc_fau_islandoraimporter_10.1088_1748-9326_11_3_034014_1632246943
Format
Citation