Current Search: Shibata, Yoshimi (x)
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Title
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The effects of leptin on glucocorticoid sensitivity of peripheral blood mononuclear cells in non-obese and obese subjects.
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Creator
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Randazzo, Christopher, Huang, Chun-Jung, Shibata, Yoshimi
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Date Issued
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2012-04-06
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PURL
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http://purl.flvc.org/fcla/dt/3349045
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Subject Headings
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Leptin, Glucocorticoid sensitivity, Obesity --epidemiology, Leptin-induced cytokine production, Peripheral blood mononuclear cells (PBMCs)
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Format
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Document (PDF)
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Title
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LPS-activated obese human PBMCs produce a BDNF and IL-6 associative response.
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Creator
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Wilson, Alan, Mari, David, Shibata, Yoshimi, Huang, Chun-Jung
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Date Issued
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2013-04-05
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PURL
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http://purl.flvc.org/fcla/dt/3361231
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Subject Headings
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Lipopolysaccharides, Obesity, Neurodegenerative diseases, IL-6 (Biomolecule)
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Format
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Document (PDF)
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Title
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Chitin Microparticles (CMPs) Induce M1 Macrophage Activation via Intracellular TLR2 Signaling Mechanism.
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Creator
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Davis, Spring, Shibata, Yoshimi, Florida Atlantic University, Charles E. Schmidt College of Medicine, Department of Biological Sciences
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Abstract/Description
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Chitin Microparticles (CMPs, 1-10um), a special form of the ubiquitous and nontoxic polysaccharide Chitin (GlcNAc), is capable of inducing a switch in macrophages from the wound-healing M2 phenotype to the classically activated pro-inflammatory M1 phenotype; which has therapeutic implications in allergy and cancer. We hypothesized that TLR2 forms a complex with CMPs and Chitin-Binding Proteins (CBPs) at the surface of peritoneal macrophages and remains with that complex after internalization...
Show moreChitin Microparticles (CMPs, 1-10um), a special form of the ubiquitous and nontoxic polysaccharide Chitin (GlcNAc), is capable of inducing a switch in macrophages from the wound-healing M2 phenotype to the classically activated pro-inflammatory M1 phenotype; which has therapeutic implications in allergy and cancer. We hypothesized that TLR2 forms a complex with CMPs and Chitin-Binding Proteins (CBPs) at the surface of peritoneal macrophages and remains with that complex after internalization to initiate downstream signaling events, leading to the production of the M1 cytokine, TNFalpha. Our results from experiments performed in RAW 264.7 cells show that TLR2 and TLR1, but not TLR6, are associated with the CMP binding fraction, and that both TLR1 and TLR2 might be important for M1 activation as a result of CMP phagocytosis. This project sheds light on CMP as a potential therapeutic agent and provides more evidence for a phagocytosis-dependent TLR2 signaling pathway.
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Date Issued
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2016
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PURL
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http://purl.flvc.org/fau/fd/FA00004762, http://purl.flvc.org/fau/fd/FA00004762
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Subject Headings
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Biopharmaceutics., Macrophages., Cell receptors., Ligands (Biochemistry), High performance processors.
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Format
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Document (PDF)