Current Search: Raghani, Anil Ratilal. (x)
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Title
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Evaluation of alumina-based stationary phases for the separations of proteins and peptides by high performance liquid chromatography.
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Creator
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Raghani, Anil Ratilal., Florida Atlantic University, Haky, Jerome E.
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Abstract/Description
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Alumina-based stationary phases are evaluated for the separations of proteins and peptides by reversed phase high performance liquid chromatography. Separations are compared to those obtained on a widely-used octadecylsilane (ODS) phase. The separations of peptides on alumina-based stationary phases are found to be superior while separations of proteins are inferior as compared to those found on ODS phase. The superior performance of peptide separations on alumina-based columns is attributed...
Show moreAlumina-based stationary phases are evaluated for the separations of proteins and peptides by reversed phase high performance liquid chromatography. Separations are compared to those obtained on a widely-used octadecylsilane (ODS) phase. The separations of peptides on alumina-based stationary phases are found to be superior while separations of proteins are inferior as compared to those found on ODS phase. The superior performance of peptide separations on alumina-based columns is attributed to lower pore size and uniquely-shaped particles of the alumina. The retentions of peptides and proteins on both alumina and silica-based stationary phases are shown to be governed by hydrophobic interaction mechanisms.
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Date Issued
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1991
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PURL
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http://purl.flvc.org/fcla/dt/14719
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Subject Headings
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Proteins--Separation, High performance liquid chromatography
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Format
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Document (PDF)