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Naturally mosaic operons for secondary metabolite biosynthesis: variability and putative horizontal transfer of discrete catalytic domains of the epothilone polyketide synthase locus
Title
Naturally mosaic operons for secondary metabolite biosynthesis: variability and putative horizontal transfer of discrete catalytic domains of the epothilone polyketide synthase locus.
Creator
Lopez, Jose V., Harbor Branch Oceanographic Institute
Date Issued
2003
PURL
http://purl.flvc.org/FCLA/DT/3166884
Subject Headings
Polyketides, Operons, Peptides --Physiological effect, Genetic transformation, Phylogeny
Format
Document (PDF)
The many faces of gene expression profiling: transcriptome analyses applied towardselucidating marine organismal interactions and metabolism
Title
The many faces of gene expression profiling: transcriptome analyses applied towardselucidating marine organismal interactions and metabolism.
Creator
Lopez, Jose V., Mouzon, B., McCarthy, Peter J., Kerr, Russell G.
Date Issued
2009
PURL
http://purl.flvc.org/fau/fd/FA00007286
Subject Headings
Gene expression, Transcriptome, Molecular biotechnology, Marine ecology, Marine organisms
Format
Document (PDF)
Genetic variation of the scleractinian coral Stylophora pistillata, from western Pacific reefs
Title
Genetic variation of the scleractinian coral Stylophora pistillata, from western Pacific reefs.
Creator
Takabayashi, Misaki, Carter, D. A., Lopez, Jose V., Hoegh-Guldberg, O., Harbor Branch Oceanographic Institute
Date Issued
2002
PURL
http://purl.flvc.org/FCLA/DT/2795917
Subject Headings
Coral reefs and islands --Pacific Ocean, Phylogenetics, Scleractinia, Nucleotide sequence, Nucleotides --Analysis
Format
Document (PDF)
Molecular studies of Caribbean biodiversity in the field: Indian River Lagoon, Florida and AndrosIsland, Bahamas
Title
Molecular studies of Caribbean biodiversity in the field: Indian River Lagoon, Florida and AndrosIsland, Bahamas.
Creator
Lopez, Jose V., Peterson, C. L., Morales, F., Brown, L., Harbor Branch Oceanographic Institute
Date Issued
2000
PURL
http://purl.flvc.org/fau/fd/FA00007395
Subject Headings
Indian River (Fla. : Lagoon), Andros Island (Bahamas), Biodiversity, Biodiversity--Research
Format
Document (PDF)
AndrosIsland flora and fauna in the new millennium
Title
AndrosIsland flora and fauna in the new millennium.
Creator
Lopez, Jose V., Peterson, C. L., Morales, F., Brown, L., Harbor Branch Oceanographic Institute
Date Issued
2000
PURL
http://purl.flvc.org/fau/fd/FA00007353
Subject Headings
Andros Island (Bahamas), Flora and fauna, Biodiversity
Format
Document (PDF)
Molecular techniques reveal wide phyletic diversity of heterotrophic microbes associated with Discodermia spp. (Porifera: Demospongiae)
Title
Molecular techniques reveal wide phyletic diversity of heterotrophic microbes associated with Discodermia spp. (Porifera: Demospongiae).
Creator
Lopez, Jose V., McCarthy, Peter J., Janda, Kathleen E., Willoughby, Robin, Pomponi, Shirley A.
Date Issued
1999
PURL
http://purl.flvc.org/fau/fd/FA00007312
Subject Headings
Porifera, Demospongiae, Microbial diversity, Genes, rRNA, Symbiosis
Format
Document (PDF)
Comparison of bacterial diversity within the coral reef sponge, Axinella corrugata, the encrusting coral Erythropodium caribaeorum
Title
Comparison of bacterial diversity within the coral reef sponge, Axinella corrugata, the encrusting coral Erythropodium caribaeorum.
Creator
Lopez, Jose V., Ranzer, L. K., Ledger, Angela, Schoch, B., Duckworth, Alan R., McCarthy, Peter J., Kerr, Russell G.
Date Issued
2008
PURL
http://purl.flvc.org/fau/fd/FA00007036
Subject Headings
Sponges, Corals, Heterotrophic bacteria, Symbiosis, RNA, Ribosomal, 16S
Format
Document (PDF)
A molecular systematic survey of sponge derived microbes
Title
A molecular systematic survey of sponge derived microbes.
Creator
Sandell, K., Peterson, C. L., Harmody, Dedra K., McCarthy, Peter J., Pomponi, Shirley A., Lopez, Jose V.
Date Issued
2004
PURL
http://purl.flvc.org/fau/fd/FA00007425
Subject Headings
Sponges, Marine microbiology, Symbiosis, Polymorphism, Restriction Fragment Length, DNA Fingerprinting
Format
Document (PDF)
Metabolites from the Marine-Derived Fungus Chromocleista sp. Isolated from a Deep-Water Sediment Sample Collected in the Gulf of Mexico
Title
Metabolites from the Marine-Derived Fungus Chromocleista sp. Isolated from a Deep-Water Sediment Sample Collected in the Gulf of Mexico.
Creator
Park, Young Chul, Gunasekera, Sarath P., Lopez, Jose V., McCarthy, Peter J., Wright, Amy E.
Date Issued
2006
PURL
http://purl.flvc.org/FCLA/DT/3164115
Subject Headings
Metabolites, Fungal metabolites, Marine fungi, X-ray crystallography, Marine sediments
Format
Document (PDF)
HBMMD: an enhanced database of the microorganisms associated with deeper water marine invertebrates
Title
HBMMD: an enhanced database of the microorganisms associated with deeper water marine invertebrates.
Creator
Gunasekera, Aravinda S., Sfanos, Karen S., Harmody, Dedra K., Pomponi, Shirley A., McCarthy, Peter J., Lopez, Jose V.
Date Issued
2004
PURL
http://purl.flvc.org/FCLA/DT/2796029
Subject Headings
Marine microbiology, Microorganisms, Marine invertebrates, Bioactive compounds, Sponges
Format
Document (PDF)
In vitro production of marine-derived antitumor compounds
Title
In vitro production of marine-derived antitumor compounds.
Creator
Pomponi, Shirley A., Willoughby, Robin, Wright, Amy E., Pecorella, C., Sennett, Susan H., Lopez, Jose V., Samples, Gail A.
Date Issued
1998
PURL
http://purl.flvc.org/fau/fd/FA00007238
Subject Headings
Antineoplastic agents, Bioactive compounds, Marine natural products, In vitro, Antitumor agents
Format
Document (PDF)
Polyketide synthase genes from marine dinoflagellates
Title
Polyketide synthase genes from marine dinoflagellates.
Creator
Snyder, Richard V., Gibbs, P. D. L., Palacios, A., Abiy, L., Dickey, R., Lopez, Jose V., Rein, Kathleen S., Harbor Branch Oceanographic Institute
Date Issued
2003
PURL
http://purl.flvc.org/FCLA/DT/2795618
Subject Headings
Dinoflagellates --Physiology, Carbonyl compounds, Polyketides, Cell biology, Enzyme activation
Format
Document (PDF)
Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis
Title
Localization of polyketide synthase encoding genes to the toxic dinoflagellate Karenia brevis.
Creator
Snyder, Richard V., Guerrero, Maria A., Sinigalliano, Christopher D., Winshell, Jamie, Perez, Roberto, Lopez, Jose V., Rein, Kathleen S., Harbor Branch Oceanographic Institute
Date Issued
2005
PURL
http://purl.flvc.org/FCLA/DT/2827832
Subject Headings
Polyketides, Dinoflagellates, Fossil --Identification, Seafood --Contamination, Seafood --Microbiology, Microbial ecology
Format
Document (PDF)
Use of real-time qPCR to quantify members of the unculturable heterotrophic bacterial community in a deep sea marine sponge, Vetulina sp
Title
Use of real-time qPCR to quantify members of the unculturable heterotrophic bacterial community in a deep sea marine sponge, Vetulina sp.
Creator
Cassler, M., Peterson, C. L., Ledger, Angela, Pomponi, Shirley A., Wright, Amy E., Winegar, R., McCarthy, Peter J., Lopez, Jose V.
Date Issued
2008
PURL
http://purl.flvc.org/FCLA/DT/2708323
Subject Headings
Microbiology --Research, Sponges --Microbiology, Marine biology --Caribbean Area
Format
Document (PDF)
A molecular systematic survey of cultured microbial associates of deep-water marine invertebrates
Title
A molecular systematic survey of cultured microbial associates of deep-water marine invertebrates.
Creator
Sfanos, Karen S., Harmody, Dedra K., Dang, Phat, Ledger, Angela, Pomponi, Shirley A., McCarthy, Peter J., Lopez, Jose V.
Date Issued
2004
PURL
http://purl.flvc.org/FCLA/DT/2827820
Subject Headings
Molecular microbiology, Microbial ecology, Marine organisms, Microorganisms, Sponges
Format
Document (PDF)
Characterization of genetic markers for in vitro cell line identification of the marine sponge, Axinella corrugata
Title
Characterization of genetic markers for in vitro cell line identification of the marine sponge, Axinella corrugata.
Creator
Lopez, Jose V., Peterson, C. L., Willoughby, Robin, Wright, Amy E., Enright, E., Zoladz, S., Pomponi, Shirley A.
Date Issued
2002
PURL
http://purl.flvc.org/FCLA/DT/3355156
Subject Headings
Sponges--Research, Marine invertebrates, Genetic markers
Format
Document (PDF)
Population dynamics of Vibrio spp. associated with marine sponge microcosms
Title
Population dynamics of Vibrio spp. associated with marine sponge microcosms.
Creator
Hoffmann, M., Fischer, M., Ottesen, A., McCarthy, Peter J., Lopez, Jose V., Brown, E. W., Monday, S. R.
Date Issued
2010
PURL
http://purl.flvc.org/FCLA/DT/3351984
Subject Headings
Sponges, Vibrio, Microbial ecology, RNA, Ribosomal, 16S
Format
Document (PDF)
Two distinct microbial communities revealed in the sponge Cinachyrella
Title
Two distinct microbial communities revealed in the sponge Cinachyrella.
Creator
Cuvelier, Marie L., Blake, Emily, Mulheron, Rebecca, McCarthy, Peter J., Blackwelder, Patricia, Thurber, Rebecca L. Vega, Lopez, Jose V.
Date Issued
2014-11-04
PURL
http://purl.flvc.org/fau/fd/FAUIR000172
Format
Citation
Co-Cultures of Pseudomonas aeruginosa and Roseobacter denitrificans Reveal Shifts in Gene Expression Levels Compared to Solo Cultures
Title
Co-Cultures of Pseudomonas aeruginosa and Roseobacter denitrificans Reveal Shifts in Gene Expression Levels Compared to Solo Cultures.
Creator
Conway, Crystal A., Esiobu, Nwadiuto, Lopez, Jose V.
Abstract/Description
Consistent biosynthesis of desired secondary metabolites (SMs) from pure microbial cultures is often unreliable. In a proof-ofprinciple study to induce SM gene expression and production, we describe mixed “co-culturing” conditions and monitoring of messages via quantitative real-time PCR (qPCR). Gene expression of model bacterial strains (Pseudomonas aeruginosa PAO1 and Roseobacter denitrificans Och114) was analyzed in pure solo and mixed cocultures to infer the effects of interspecies...
Show moreConsistent biosynthesis of desired secondary metabolites (SMs) from pure microbial cultures is often unreliable. In a proof-ofprinciple study to induce SM gene expression and production, we describe mixed “co-culturing” conditions and monitoring of messages via quantitative real-time PCR (qPCR). Gene expression of model bacterial strains (Pseudomonas aeruginosa PAO1 and Roseobacter denitrificans Och114) was analyzed in pure solo and mixed cocultures to infer the effects of interspecies interactions on gene expression in vitro, Two P. aeruginosa genes (PhzH coding for portions of the phenazine antibiotic pathway leading to pyocyanin (PCN) and the RhdA gene for thiosulfate: cyanide sulfurtransferase (Rhodanese)) and two R. denitrificans genes (BetaLact formetallo-beta-lactamase and the DMSP gene for dimethylpropiothetin dethiomethylase) were assessed for differential expression. Results showed that R. denitrificans DMSP and BetaLact gene expression became elevated in a mixed culture. In contrast, P. aeruginosa co-cultures with R. denitrificans or a third species did not increase target gene expression above control levels. This paper provides insight for better control of target SM gene expression in vitro and bypass complex genetic engineering manipulations.
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Date Issued
2012
PURL
http://purl.flvc.org/fau/fd/FAUIR000016
Format
Citation
Deepsea coral collection protocols: a synthesis of field experience from deep-sea coral researchers, designed to build our national capacity to document deep-sea coral diversity
Title
Deepsea coral collection protocols: a synthesis of field experience from deep-sea coral researchers, designed to build our national capacity to document deep-sea coral diversity.
Creator
Etnoyer, P., Cairns, S. D., Sanchez, J. A., Reed, John K., Lopez, Jose V., Schroeder, W. W., Brooke, S. D., Watling, L., Baco-Taylor, A., Williams, G. C., Lindner, A., France, Scott C., Bruckner, A. W.
Date Issued
2006
PURL
http://purl.flvc.org/FCLA/DT/3172990
Subject Headings
Deep sea corals, Corals --Collection and preservation, Deep-sea ecology, Octocorallia, Coral reef conservation
Format
Document (PDF)

Pages