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Characterization of normal mitochondrial inheritance in C. elegans and a reverse genetic approach to identify possible genes involved
Title
Characterization of normal mitochondrial inheritance in C. elegans and a reverse genetic approach to identify possible genes involved.
Creator
Wilson, Amber Diane, Florida Atlantic University, LaMunyon, Craig W.
Abstract/Description
Mitochondria are inherited uniparentally in almost all eukaryotic models studied to date. The fathers mitochondria are eliminated and there have been several hypothesis as to how this occurs. One hypothesis is that the sperm mitochondria are actively targeted and destroyed. Ubiquitin has been proposed a possible candidate involved in this process. My research investigated the normal mitochondrial inheritance pattern in C. elegans. I also examined the possible role of the C34F11.1 gene in...
Show moreMitochondria are inherited uniparentally in almost all eukaryotic models studied to date. The fathers mitochondria are eliminated and there have been several hypothesis as to how this occurs. One hypothesis is that the sperm mitochondria are actively targeted and destroyed. Ubiquitin has been proposed a possible candidate involved in this process. My research investigated the normal mitochondrial inheritance pattern in C. elegans. I also examined the possible role of the C34F11.1 gene in mitochondrial inheritance. This gene is sperm-specific and has ubiquitin-ligase properties. It was determined that the normal mitochondrial inheritance pattern in C. elegans is maternal and that the sperm mitochondria are eliminated. It was also concluded that the C34F11.1 gene does not have a role in normal mitochondrial inheritance.
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Date Issued
2003
PURL
http://purl.flvc.org/fcla/dt/13049
Subject Headings
Caenorhabditis elegans, Mitochondrial DNA
Format
Document (PDF)
Female multiple mating and sperm storage in an arctiid moth: Utetheisa ornatrix
Title
Female multiple mating and sperm storage in an arctiid moth: Utetheisa ornatrix.
Creator
Curril, Ingrid M., Florida Atlantic University, LaMunyon, Craig W.
Abstract/Description
Utetheisa ornatrix females mate with many different males, but only one of their mates generally sires the progeny. To investigate the mechanism of this sperm precedence, the sperm contained within the spermatheca of field-caught females was counted. Captured females contained the remains of between 1 and 23 spermatophores, each received at a separate mating. The sperm number did not depend on the number of matings. These data suggests that sperm may be lost from the spermatheca, and that it...
Show moreUtetheisa ornatrix females mate with many different males, but only one of their mates generally sires the progeny. To investigate the mechanism of this sperm precedence, the sperm contained within the spermatheca of field-caught females was counted. Captured females contained the remains of between 1 and 23 spermatophores, each received at a separate mating. The sperm number did not depend on the number of matings. These data suggests that sperm may be lost from the spermatheca, and that it may never arrive there after some matings. In addition to this investigation, a study on the pattern of arrangement within the spermatheca of Utetheisa ornatrix was conducted. Sperm in the spermatheca from multiple and single mated females was fixed and DNA-labeled, and the distribution of eupyrene sperm assessed. The eupyrene sperm distribution did not vary across the sperm mass or with the mating history of the female. Further investigation is required to analyze the distribution of the apyrene sperm tangles within the spermatheca whose function could signify a blocking mechanism against rival sperm.
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Date Issued
2003
PURL
http://purl.flvc.org/fcla/dt/13100
Subject Headings
Arctiidae, Moths--Spermatozoa
Format
Document (PDF)
Mitochondrial DNA damage in C. elegans sperm as a function sperm activity
Title
Mitochondrial DNA damage in C. elegans sperm as a function sperm activity.
Creator
Willis, Joel P., Florida Atlantic University, Volin, John C., LaMunyon, Craig W.
Abstract/Description
Several models for the evolution of maternal inheritance of mitochondria predict that the sperm mitochondria undergo oxidative damage and pose a threat to the developing embryo. Here, I test the hypothesis that the sperm are damaged by reactive oxygen species generated by aerobic sperm activity. In my first experiment, I found no significant difference in fecundity between worms fertilized by old versus young sperm, suggesting that the sperm nuclear genome is not affected by the extent of...
Show moreSeveral models for the evolution of maternal inheritance of mitochondria predict that the sperm mitochondria undergo oxidative damage and pose a threat to the developing embryo. Here, I test the hypothesis that the sperm are damaged by reactive oxygen species generated by aerobic sperm activity. In my first experiment, I found no significant difference in fecundity between worms fertilized by old versus young sperm, suggesting that the sperm nuclear genome is not affected by the extent of sperm activity. In my second experiment, I found that sperm mitochondrial DNA has deletions, indicating damage, but this damage does not accumulate with sperm activity. However, problems with PCR amplification resulted in little experimental data, preventing a conclusive test of the hypothesis.
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Date Issued
2003
PURL
http://purl.flvc.org/fcla/dt/13083
Subject Headings
Caenorhabditis elegans, Worms--Spermatozoa, Worms--Genetics
Format
Document (PDF)