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Title: Anticancer activities of genistein-topotecan combination in LNCaP prostate cancer cells.
Creator: Hörmann, Vanessa P., Kumi-Diaka, James, Graduate College
Date Issued: 2011-04-08
PURL: http://purl.flvc.org/fcla/dt/3164543
Subject Headings: Prostate --Cancer --Alternative treatment, Cancer --Chemotherapy, Isoflavones --therapeutic use
Format: Document (PDF)

Title: Impact of Vitamin C on Genistein induced apoptosis in treatment of prostate cancer cells.
Creator: Famuyiwa, Toluleke, Boe, Andrew, Esiobu, Nwadiuto, Graduate College, Kumi-Diaka, James
Abstract/Description: Background: Prostate Cancer, in the absence of skin cancer, is the most prevalent type of cancer found in the male population. Reactive Oxygen Species (ROS) can promote cancer cell proliferation when they are at elevated levels. Vitamin C is a water-soluble antioxidant capable of inhibiting the formation of ROS. Genistein, an isoflavone found in plants, also possesses the ability to inhibit ROS formation. Objective To determine the potential therapeutic synergy between genistein and vitamin C...
Show moreBackground: Prostate Cancer, in the absence of skin cancer, is the most prevalent type of cancer found in the male population. Reactive Oxygen Species (ROS) can promote cancer cell proliferation when they are at elevated levels. Vitamin C is a water-soluble antioxidant capable of inhibiting the formation of ROS. Genistein, an isoflavone found in plants, also possesses the ability to inhibit ROS formation. Objective To determine the potential therapeutic synergy between genistein and vitamin C and investigate mechanism of action of genistein and/or vitamin C. Methods: Trypan blue assay was carried out to know the % of viable cells. Varying concentrations of genistein with a constant concentration of Vitamin C was used to treat LNCaP cells. After treatment of the cells with genistein and Vitamin C, MTT assay of the cancer cells was performed and absorbance read through an ELISA reader. This gives the values needed for interpreting cell viability after treatment. A statistical analysis performed to determine whether the obtained results are statistically significant. Results: The results obtained from our experiments are inconclusive with regards to the impact of Vitamin C on apoptotic cancer cell death following genistein treatment. However the combination of genistein and vitamin C was more efficient in tumor suppression than when the drugs were given separately. Conclusion: This study suggests that treatment of prostate cancer using genistein can be enhanced by adjuvant treatment with vitamin C. This study is of potential clinical success in reducing the cell death by necrosis.
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Date Issued: 2015
PURL: http://purl.flvc.org/fau/fd/FA00005876
Format: Document (PDF)

Title: Unlocking the Phytotherapeutic Synergism between Beta-lapachone and Soybean-derived Genistein in Human Prostate cancer cells.
Creator: Oseni, Saheed Oluwasina, Sandoval-Bernal, Bibiana, Kumi-Diaka, James, Graduate College
Abstract/Description: Prostate cancer after many years is still the second most common cancer in American men with about 233,000 new cases and 29,480 deaths estimated to be occurring in 2014. Despite the wide spectra of reports demonstrating the anti-cancer phytotherapeutic potentials of beta-lapachone and soybean-derived genistein in various tumors, little emphasis had been placed on their synergistic effects in androgen-independent PC3 and androgen-dependent LNCaP prostate cancer cell lines. In this study, we...
Show moreProstate cancer after many years is still the second most common cancer in American men with about 233,000 new cases and 29,480 deaths estimated to be occurring in 2014. Despite the wide spectra of reports demonstrating the anti-cancer phytotherapeutic potentials of beta-lapachone and soybean-derived genistein in various tumors, little emphasis had been placed on their synergistic effects in androgen-independent PC3 and androgen-dependent LNCaP prostate cancer cell lines. In this study, we aim to characterize the combined effects of genistein and b-lapachone on the phyto/chemosensitivity of LNCaP and PC3 human prostate cancer cells in-vitro, using MTT assay and LDH assay to study treatment-induced growth inhibition and cytotoxicity. Annexin-V-FITC and PI-TUNEL assays were also used to determine the potential treatment-induced apoptosis and/or necrosis. Our results revealed that both PC3 and LNCaP are phytosensitive to both single and combined treatments, though time-and dose-dependent. We observed that our treatments induced dual death pathways-apoptosis and necrosis-in both cell types and also observed that growth inhibition in both correlated positively with cell death in which, b-lapachone and genistein induced cell cycle arrest at the G1 and/or S phase and G2–M checkpoints respectively. Invariably, our results indicate that combination treatments with b-lapachone and genistein are more potent in killing both PC3 and LNCaP cancer cells than treatment with either genistein or b-lapachone alone. Our current results are therefore in agreement with the hypothesis that drugcombinations that target cell cycles at different critical checkpoints are more effective in causing cell death.
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Date Issued: 2014
PURL: http://purl.flvc.org/fau/fd/FA00005845
Format: Document (PDF)

Title: Pyroelectric Crystal Generated Very Low Dose X-rays Enhanced the Phytotherapeutic Effects of Beta-lapachone in Hormone Dependent Prostate Cancer Cells In Vitro.
Creator: Oseni, Saheed Oluwasina, Kumi-Diaka, James, Jebelli, Joubin, Graduate College, Goldsmith, Harris, Kaldas, George, Branly, Rolando
Abstract/Description: In 2015, an estimated 220, 800 new cases and 27, 540 deaths are expected to occur due to prostate cancer in US men, thus adding to the economic burden of the over 2.6 million men currently battling the disease. Plethora of studies have demonstrated the phytotherapeutic potentials of beta-lapachone, a phytochemical compound derived from the bark of the lapacho tree, native to South America. Betalapachone (β-lap) has been shown to exhibit its anti-cancer effects majorly by the futile cycling...
Show moreIn 2015, an estimated 220, 800 new cases and 27, 540 deaths are expected to occur due to prostate cancer in US men, thus adding to the economic burden of the over 2.6 million men currently battling the disease. Plethora of studies have demonstrated the phytotherapeutic potentials of beta-lapachone, a phytochemical compound derived from the bark of the lapacho tree, native to South America. Betalapachone (β-lap) has been shown to exhibit its anti-cancer effects majorly by the futile cycling between the oxidized and the two electron reduction of β-lap mediated by NAD(P)H:quinone oxidoreductase (NQO1) resulting in the generation of reactive oxygen species (ROS) using NADH or NAD(P) as electron sources. β-lap is known to selectively kill human cancer cells, since NQO1 is expressed more abundantly in numerous human solid tumors than in the adjacent normal tissues; NQO1 has been shown to be exceptionally under expressed in hormone dependent prostate cancer cells (LNCaP) compared to the hormone independent prostate cancer cells (PC3). This study was aimed to investigate the enhancing effects of very low dose radiation (VLDR (20mGy)) derived from a pyroelectric crystal generator on the phytotherapeutic activity of beta-lapachone in LNCaP cell line in vitro accessed by MTT and Trypan blue assay. Treatment-induced intracellular levels of ROS were also assessed using Nitro blue tetrazolium assay. NQO1 activities in LNCaP cells were also investigated following treatment with VLDR and/or β-lap using Dicoumarol (NQO1 inhibitor). Results indicate that LNCaP cells respond significantly to combined treatments compared to single treatments.
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Date Issued: 2015
PURL: http://purl.flvc.org/fau/fd/FA00005905
Format: Document (PDF)

Title: Potential mechanism of phytochemical-induced apoptosis in human prostate adenocarcinoma cells: Therapeutic synergy in genistein and β-lapachone combination treatment.
Creator: Kumi-Diaka, James, Saddler, Shawnette Simone, Aller, Alex, Brown, Jayann Marie
Date Issued: 2004-08-17
PURL: http://purl.flvc.org/fcla/dt/3327156
Format: Document (PDF)

Title: Anticancer activity of two dietary phytochemicals: Genistein and beta-lapachone.
Creator: Merchant, Kendra T., Florida Atlantic University, Kumi-Diaka, James
Abstract/Description: Phytochemicals are biologically active secondary plant metabolites that have been shown to exhibit anti-cancer activity. The dietary phytochemicals genistein isoflavone and beta-lapachone, were investigated to determine their effect on the growth of human prostate adenocarcinoma cells in vitro. The cells were exposed to varying concentrations of both phytochemicals in single and combination treatments for specified time periods and their effect was determined using post-treatment cell...
Show morePhytochemicals are biologically active secondary plant metabolites that have been shown to exhibit anti-cancer activity. The dietary phytochemicals genistein isoflavone and beta-lapachone, were investigated to determine their effect on the growth of human prostate adenocarcinoma cells in vitro. The cells were exposed to varying concentrations of both phytochemicals in single and combination treatments for specified time periods and their effect was determined using post-treatment cell viability, treatment-induced apoptosis and cell signaling assays. The overall results revealed that both phytochemicals inhibited cell growth and proliferation and induced apoptosis in a dose-dependent manner for both single and combination treatments. However, combination treatments were not significantly more effective than single treatment with either drug. Both phytochemicals could therefore offer therapeutic efficacy in human prostate adenocarcinoma.
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Date Issued: 2005
PURL: http://purl.flvc.org/fcla/dt/13250
Subject Headings: Phytochemicals--Physiological effect, Prostate--Cancer--Molecular aspects, Apoptosis--Molecular aspects, Prostate--Cancer--Treatment
Format: Document (PDF)

Title: Anti-cancer activity of pomegranate (Punica granatum ) extracts in testicular cancer.
Creator: Brown, Jayann Marie, Florida Atlantic University, Kumi-Diaka, James
Abstract/Description: Recent advancement in chemotherapy has resulted in higher and longer survival rates of testicular cancer patients. However the use of chemotherapeutic agents are not without serious, sometimes fatal side effects. This study investigated the potential therapeutic efficacy of pomegranate extracts in testis cancer cells, GC1-spg, in vitro. A battery of assays was used to determine the chemosensitivity of GC1-spg cells to two pomegranate extracts, S (seed) and P (pericarp), in single and...
Show moreRecent advancement in chemotherapy has resulted in higher and longer survival rates of testicular cancer patients. However the use of chemotherapeutic agents are not without serious, sometimes fatal side effects. This study investigated the potential therapeutic efficacy of pomegranate extracts in testis cancer cells, GC1-spg, in vitro. A battery of assays was used to determine the chemosensitivity of GC1-spg cells to two pomegranate extracts, S (seed) and P (pericarp), in single and combination treatments: MTS and LDH to determine post-treatment survival rate (growth inhibition) and cytotoxicity respectively; Acridine Orange/Ethidium Bromide fluorescent dye to assess treatment-induced apoptosis/necrosis; Annexin V-FITC and TUNEL assays for early and late apoptosis respectively. Results from the obtained data indicated that both extracts have significant cytotoxic effect on testicular cancer cells (GC1-spg) in single and combination treatments. The data revealed a dose and time dependency of chemosensitivity to both extracts; and that apoptosis was the major mechanism treatment-induced cell death. Synergism was also indicated in growth inhibition by combination treatment. These findings offer strong justification for further studies with pomegranate as potential phytotherapy.
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Date Issued: 2004
PURL: http://purl.flvc.org/fcla/dt/13154
Subject Headings: Testis--Cancer--Treatment, Generative organs, Male--Diseases--Treatment, Phytochemicals--Physiological effect, Cancer--Adjuvant treatment, Apoptosis--Molecular aspects
Format: Document (PDF)

Title: Potential mechanism of phytochemical-induced apoptosis in human prostate cancer cells: Genistein and beta-lapachone.
Creator: Saddler, Shawnette Simone, Florida Atlantic University, Kumi-Diaka, James
Abstract/Description: The present study was undertaken to determine the chemotherapeutic potential of genistein and beta-lapachone and possible mechanisms of action in prostate cancer in vitro. The bioassays used included: MTT and LDH chemosensitivity-cytotoxicity assays, NQO1 detection, annexin V-FITC, TUNEL and the caspase protease (CPP32) apoptotic detection assays. The results showed that: (i) PC3 cells are sensitive to single and combination treatments in a dose and time dependent manner; (ii) there was...
Show moreThe present study was undertaken to determine the chemotherapeutic potential of genistein and beta-lapachone and possible mechanisms of action in prostate cancer in vitro. The bioassays used included: MTT and LDH chemosensitivity-cytotoxicity assays, NQO1 detection, annexin V-FITC, TUNEL and the caspase protease (CPP32) apoptotic detection assays. The results showed that: (i) PC3 cells are sensitive to single and combination treatments in a dose and time dependent manner; (ii) there was treatment-induced dual death pathways (apoptosis and necrosis) with increasing toxicity (necrosis) at higher concentrations in single and combination treatments; (iii) combination treatment was more growth inhibitory than single treatments; (iv) the NQO1 enzyme substantially enhances the toxicity of beta-lapachone but not genistein, while genistein exerted its apoptotic inducing effects via the caspase 3 pathway. The overall results indicate that combination treatments with beta-lapachone and genistein are more efficacious in killing PC3 human prostate cancer cells than treatment with either agent alone.
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Date Issued: 2003
PURL: http://purl.flvc.org/fcla/dt/13042
Subject Headings: Prostate--Cancer--Cytopathology, Apoptosis, Prostate--Cancer--Molecular aspects
Format: Document (PDF)

Title: Therapeutic potential of pomegranate and genistein for human breast cancer.
Creator: Louis Jeune, Marie Adeline, Florida Atlantic University, Kumi-Diaka, James
Abstract/Description: The therapeutic potential of pomegranate and genistein on growth inhibition of human breast cancer cells was investigated. Methods. Cells (MCF-7) were initially cultured for 48 hr to achieve 80% confluence; and then exposed to the agents in single and combination treatments. Post-treatment analysis was done by using a series of bioassays, including LDH, MTS, AcrO-EthBr, Annexin-FITC and TUNEL assays for growth inhibition and apoptosis detection; and Caspase-3 and NQO1 for molecular pathways...
Show moreThe therapeutic potential of pomegranate and genistein on growth inhibition of human breast cancer cells was investigated. Methods. Cells (MCF-7) were initially cultured for 48 hr to achieve 80% confluence; and then exposed to the agents in single and combination treatments. Post-treatment analysis was done by using a series of bioassays, including LDH, MTS, AcrO-EthBr, Annexin-FITC and TUNEL assays for growth inhibition and apoptosis detection; and Caspase-3 and NQO1 for molecular pathways of apoptosis. Results. Pomegranate and genistein showed significant dose- and time-dependent cytotoxic and growth inhibition effects as well as apoptosis induction in MCF-7 cancer cells, with significantly higher ( P < 0.01) effects in the combination treatments than in the single treatments. Both drugs induced apoptosis through a caspase-mediated mechanism and independent of NQO1. Discussion and conclusions. Pomegranate and genistein inhibit the growth of MCF-7 breast cancer cells through induction of apoptosis with combination treatment being more efficacious than single treatments.
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Date Issued: 2004
PURL: http://purl.flvc.org/fcla/dt/13130
Subject Headings: Phytochemicals--Physiological effect, Breast--Cancer--Molecular aspects, Women--Diseases--Alternative treatment, Apoptosis--Molecular aspects, Breast--Cancer--Treatment
Format: Document (PDF)

Title: Genistein targets only proliferating but not quiescent cells: Potential therapeutic significance in breast cancer.
Creator: Bodepudi, Sreedevi., Florida Atlantic University, Kumi-Diaka, James
Abstract/Description: Phytochemicals are biologically active secondary plant metabolites that could mimic biological activities. In this study genistein isoflavone, a phytochemical present in soy was investigated to determine its effect on the growth of human breast cancer cell line GI-101 and normal breast epithelial cells in vitro. The cells were exposed to varying concentrations of genistein isoflavone for 24 and 48 hour time periods and the effect was determined using post-treatment assays: MTT and Trypan Blue...
Show morePhytochemicals are biologically active secondary plant metabolites that could mimic biological activities. In this study genistein isoflavone, a phytochemical present in soy was investigated to determine its effect on the growth of human breast cancer cell line GI-101 and normal breast epithelial cells in vitro. The cells were exposed to varying concentrations of genistein isoflavone for 24 and 48 hour time periods and the effect was determined using post-treatment assays: MTT and Trypan Blue for cell viability; LDH assay for cytotoxicity; Rhodamine 123/Propidium Iodide and Ethidium Bromide/Acridine Orange assays for treatment-induced apoptosis and FAM Poly caspase assay for mechanism of action. The overall results revealed that genistein inhibited cell growth and proliferation through apoptosis in the cells in both time and dose-dependent manner. Normal breast epithelial cells were not significantly affected by genistein at the corresponding dosages. Based on the results obtained, it was concluded that genistein isoflavone could offer therapeutic efficacy in human breast carcinoma without significantly affecting the normal breast epithelial cells.
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Date Issued: 2006
PURL: http://purl.flvc.org/fcla/dt/13315
Subject Headings: Phytochemicals--Physiological effect, Breast--Cancer--Molecular aspects, Women--Diseases--Alternative treatment, Breast--Cancer--Treatment, Apoptosis--Molecular aspects
Format: Document (PDF)

Title: Chemopreventive Effects of Magnesium Chloride Supplementation on Hormone Independent Prostate Cancer Cells.
Creator: Saheed Oluwasina Oseni, Elsa Quiroz, James Kumi-Diaka
Abstract/Description: Lifestyle significantly impacts the risk factors associated with prostate cancer, out of which diet appears to be the most influential. An emerging chemopreventive approach, which involves the adequate intake of dietary constituents, has shown great potential in preventing the occurrence or progression of cancer. Magnesium is known to be an essential cofactor for more than 300 enzymatic processes, and is responsible for the regulation of various cellular reactions in the body. A plethora of...
Show moreLifestyle significantly impacts the risk factors associated with prostate cancer, out of which diet appears to be the most influential. An emerging chemopreventive approach, which involves the adequate intake of dietary constituents, has shown great potential in preventing the occurrence or progression of cancer. Magnesium is known to be an essential cofactor for more than 300 enzymatic processes, and is responsible for the regulation of various cellular reactions in the body. A plethora of studies have shown evidence that changes in the intracellular levels of magnesium could contribute to cell proliferation and apoptosis in some normal and malignant cells. The aim of the study was to investigate the effects of magnesium chloride (MgCl2) in DU-145 prostate cancer cells.
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Date Issued: 2016
PURL: http://purl.flvc.org/fau/fd/FAUIR000486
Format: Document (PDF)

Title: CytoregR inhibits growth and proliferation of human adenocarcinoma cells via induction of apoptosis.
Creator: Kumi-Diaka, James, Hassanhi, M., Brown, Jayann Marie, Merchant, Kendra T., Garcia, C., Jimenez, W.
Date Issued: 2006-01-09
PURL: http://purl.flvc.org/fcla/dt/3327155
Format: Document (PDF)

Title: Anticancer ativities of topotecan-genistein combination in prostate cancer cells.
Creator: Hörmann, Vanessa P., Kumi-Diaka, James, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: Prostate cancer is one of the leading causes of death in men aged 40-55. Genistein isoflavone (4', 5', 7-trihydroxyisoflavone) is a dietary phytochemical with demonstrated anti-tumor activities in a variety of cancers. Topotecan Hydrochloride (Hycamtin) is an FDA-approved chemotherapy drug, primarily used for secondary treatment of ovarian,cervical and small cell lung cancers. This study was to demonstrate the potential anticancer activities and synergy of topotecan-genistein combination in...
Show moreProstate cancer is one of the leading causes of death in men aged 40-55. Genistein isoflavone (4', 5', 7-trihydroxyisoflavone) is a dietary phytochemical with demonstrated anti-tumor activities in a variety of cancers. Topotecan Hydrochloride (Hycamtin) is an FDA-approved chemotherapy drug, primarily used for secondary treatment of ovarian,cervical and small cell lung cancers. This study was to demonstrate the potential anticancer activities and synergy of topotecan-genistein combination in LNCaP prostate cancer cells. The potential efficacy and mechanism of topotecan/genistein-induced cell death was investigated... Results: The overall data indicated that i) both genistein and topotecan induce cellular death in LNCaP cells, ii) topotecan-genistein combination was significantly more efficacious in reducing LNCaP cell viabiligy compared to either genistein or topotecan alone, iii) in all cases, cell death was primarily through apoptosis, via the activation of the intrinsic pathway, iv) ROS levels were increased and VEGF expression was diminished significantly with the topotecan-genistein combination treatment, v) genetic analysis of topotecan-genistein treatment groups showed changes in genetic expression levels in pathway specific apoptotic genes.... Conclusion: Treatments involving topotecan-genistein combination may prove to be an attractive alternative phytotherapy of adjuvant therapy for prostate cancer.
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Date Issued: 2012
PURL: http://purl.flvc.org/FAU/3358553
Subject Headings: Apoptosis, Molecular aspects, Prostate, Cancer, Adjuvant treatment, Prostate, Cancer, Molecular aspects, Phytochemicals, Physiological effect, Antioxidants, Therapeutic use, Topotecan, Therapeutic use, Genistein, Therapeutic use, Cancer, Chemotherapy
Format: Document (PDF)

Title: GENOTYPIC SPERM SORTING: A less invasive “ART” to prevent Genetic Disorders in Newborns.
Creator: Adenmosun, Olumide O., Kumi-Diaka, James, Asghar, Waseem, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Genetic disorders like Cystic Fibrosis (CF) and X-linked Diseases (XLD) are inherited by offspring from parents who are healthy carriers of the autosomal recessive or allosomal genes. About 10-million Americans are healthy carriers of a mutant cysticfibrosis gene (predominantly F508del) and about 4% of newborns are at risk of being born with an X-linked disease. The current clinically approved mitigation plan for preventing genetic disorders in newborns from “at-risk couples” is to consider...
Show moreGenetic disorders like Cystic Fibrosis (CF) and X-linked Diseases (XLD) are inherited by offspring from parents who are healthy carriers of the autosomal recessive or allosomal genes. About 10-million Americans are healthy carriers of a mutant cysticfibrosis gene (predominantly F508del) and about 4% of newborns are at risk of being born with an X-linked disease. The current clinically approved mitigation plan for preventing genetic disorders in newborns from “at-risk couples” is to consider Preimplantation Genetic Testing for Monogenetic diseases (PGT-M). PGT-M involves an invasive microsurgical procedure that requires the removal of cells from 3-5day old embryos. To minimize this invasiveness, we proposed a less invasive approach to prevent genetic disorders in newborns by genotypically sorting sperm cells which may be used for fertilization events (IUI/IVF/ICSI) with specially characterized antigens on the sperm surface membrane. For the disease models being adopted in our study – CF and XLD; we utilized certain monoclonal antibodies (mab) to target the H-Y male antigen and the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) protein which are both selectively expressed on the sperm surface.
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Date Issued: 2021
PURL: http://purl.flvc.org/fau/fd/FA00013805
Subject Headings: Genetic disorders--Prevention, Genetic Testing, Reproductive technology, Cystic fibrosis
Format: Document (PDF)

Title: Enhancement of the Chemopreventive and Chemotherapeutic Effects of Genistein and Beta-lapachone in Human Prostate Cancer Cells by Pyroelectrically Generated Very Low Dose Ionizing Radiation.
Creator: Oseni, Saheed Oluwasina, Kumi-Diaka, James, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: An estimated 220,800 new prostate cancer cases and 27,540 deaths are expected to occur in US men by the end of 2015. Despite the increased treatment modes for prostate cancer, there is still no definite cure, and prognosis remains, at best, cautiously optimistic. The explicit amalgamation of two or more cancer therapeutic modalities such as surgery, radiation, and chemotherapy, has been one of the main interests of clinical investigation for several decades. Genistein (GN) and Beta-lapachone ...
Show moreAn estimated 220,800 new prostate cancer cases and 27,540 deaths are expected to occur in US men by the end of 2015. Despite the increased treatment modes for prostate cancer, there is still no definite cure, and prognosis remains, at best, cautiously optimistic. The explicit amalgamation of two or more cancer therapeutic modalities such as surgery, radiation, and chemotherapy, has been one of the main interests of clinical investigation for several decades. Genistein (GN) and Beta-lapachone (BL) are two of the most promising anticancer phytochemical compounds. However, the anticancer activities of BL have been correlated with the enzyme activity of NQO1. The aim of this study was to investigate the enhancing effects of VLDR derived from a portable pyroelectric crystal generator on the chemopreventive and/or chemotherapeutic effects of GN and BL in NQO1+ PC3 and NQO1± (deficient) LNCaP prostate cancer cells (PCa) in vitro. The combination treat ment-induced cytotoxicity was investigated via MTT and Trypan blue exclusion assays. Dicoumarol (an NQO1 inhibitor) was co-administered to assess the effect of VLDR on NQO1 modulation. Nitro-blue tetrazolium assay was used to assess the intracellular ROS levels. Fluorescence microscopy was also used to assess the mode of cell death. In this study, a novel quantitative modeling approach was employed to comparably assess the cytotoxic effects of specific drugs used alone or in combinations with VLDR and to predict the potential synergistic therapeutic combinations. The data suggests that VLDR induced a rise in ROS levels, followed by upregulation in NQO1 levels. Pharmacodynamic indices were developed to quantify and characterize the combination treatment as synergistic, additive or antagonistic per dose or time-interval. Synergism was found to be dose and time-interval dependent. The major mode of cell death by this combination therapeutic regimen was found to be via apoptosis . In conclusion, our results confirm that VLDR enhanced cytotoxicity effects of both drugs dose- and time-dependently.
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Date Issued: 2015
PURL: http://purl.flvc.org/fau/fd/FA00004530, http://purl.flvc.org/fau/fd/FA00004530
Subject Headings: Apoptosis -- Molecular aspects, Genistein -- Therapeutic use, Phytochemicals -- Physiological effect, Phytochemicals -- Therapeutic use, Prostate -- Cancer -- Adjuvant treatment, Prostate -- Cancer -- Cryptopathology, Prostate -- Cancer -- Molecular aspects
Format: Document (PDF)

Title: Impact of Vitamin C on Genistein-Induced Apoptosis in Prostate Cancer.
Creator: Famuyiwa, Toluleke, Kumi-Diaka, James, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: This study determined the impact of vitamin C dose on genistein-induced apoptosis in LNCaP cancer cells at various treatment regimens in vitro. Although the linear regression of viability assay (MTT) indicated a p-value = 0.11; NBT assay reveal a declining SOD activity during cell death. Apoptosis induction was the main mode of treatment induced cell death. The overall data showed the trend of treatment efficacy as;(Gen 10uM + Vit C 40uM) > (Gen 30uM + Vit C 40uM) > (Gen 70uM + Vit C 40uM) >...
Show moreThis study determined the impact of vitamin C dose on genistein-induced apoptosis in LNCaP cancer cells at various treatment regimens in vitro. Although the linear regression of viability assay (MTT) indicated a p-value = 0.11; NBT assay reveal a declining SOD activity during cell death. Apoptosis induction was the main mode of treatment induced cell death. The overall data showed the trend of treatment efficacy as;(Gen 10uM + Vit C 40uM) > (Gen 30uM + Vit C 40uM) > (Gen 70uM + Vit C 40uM) > 10uM genistein > 70uM genistein. The chi-square test for comparing necrosis, apoptosis and life cells showed that Vitamin C could impact genistein-induced apoptosis in LNCaP cells (p = 0.0003). This study forms the basis for in vivo studies of the impact of vitamin C on genistein-induced apoptosis in LNCaP prostate cancer cells.
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Date Issued: 2015
PURL: http://purl.flvc.org/fau/fd/FA00004497, http://purl.flvc.org/fau/fd/FA00004497
Subject Headings: Apoptosis -- Molecular aspects, Cellular signal transduction, Genistein -- Therapeutic use, Phytochemicals -- Physiological effect, Phytochemicals -- Therapeutic use, Prostate -- Cancer -- Adjuvant treatment, Prostate -- Cancer -- Molecular aspects, Vitamin C -- Therapeutic use
Format: Document (PDF)

Title: Therapeutic Options for the Treatment of Breast Cancer: Using Cytoreg and Genistein Isoflavone.
Creator: Johnson, Michelle M., Kumi-Diaka, James, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
Abstract/Description: In spite the heavy investments in therapeutic research breast cancer still impacts the lives of women globally. The projected incidence of new cases in USA for 2008 is 67,770, with estimated 40,480 deaths. In this study, we investigated the therapeutic efficacy of Cytoreg®-genistein combination treatment on MCF-7 human breast cancer cells. MCF-7 cells were treated with genistein and Cytoreg® single and combination treatments for 24- 48hr; and the chemosensitivity assessed using bioassays:...
Show moreIn spite the heavy investments in therapeutic research breast cancer still impacts the lives of women globally. The projected incidence of new cases in USA for 2008 is 67,770, with estimated 40,480 deaths. In this study, we investigated the therapeutic efficacy of Cytoreg®-genistein combination treatment on MCF-7 human breast cancer cells. MCF-7 cells were treated with genistein and Cytoreg® single and combination treatments for 24- 48hr; and the chemosensitivity assessed using bioassays: Trypan Blue and MTT for cell viability; Ethidium bromide/Rhodamine 123 to assess apoptosis induction; F AM PolyCaspase binding assay for mechanism of action. The overall data indicated dose- and timedependent cell death in the MCF-cells and that apoptosis was the major means of treatmentinduced growth inhibition. There was evidence of Cytoreg®-induced autophagy in the cells. The overall findings indicated that genistein-Cytoreg® combination was more efficacious than either genistein or Cytoreg® alone. Cytoreg® enhanced the phytosensitivity of MCF-7 cells to genistein isoflavone.
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Date Issued: 2008
PURL: http://purl.flvc.org/fau/fd/FA00000777
Subject Headings: Breast--Cancer--Treatment, Phytochemicals--Physiological effect, Apoptosis--Molecular aspects, Phytoestrogens--Health aspects, Outcome assessment (Medical care)
Format: Document (PDF)

Title: ROLE OF INTERLEUKIN-1 RECEPTOR-ASSOCIATED KINASES IN CHRONIC INFLAMMATION AND PROSTATE TUMORIGENESIS.
Creator: Oseni, Saheed Oluwasina, Kumi-Diaka, James, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: The oncogenic role of many of inflammatory genes in prostate cancer (PCa) remains unexplored despite the increasing association of chronic inflammation with PCa initiation, progression, and therapy resistance. The overarching goal of this project was to identify dysregulated inflammatory genes that correlate with PCa progression and seek to understand their molecular mechanisms and the therapeutic potential of targeting them. To achieve this, we utilized cutting-edge integrative (epi) genomic...
Show moreThe oncogenic role of many of inflammatory genes in prostate cancer (PCa) remains unexplored despite the increasing association of chronic inflammation with PCa initiation, progression, and therapy resistance. The overarching goal of this project was to identify dysregulated inflammatory genes that correlate with PCa progression and seek to understand their molecular mechanisms and the therapeutic potential of targeting them. To achieve this, we utilized cutting-edge integrative (epi) genomic and transcriptomic techniques to identify and characterize inflammatory genes whose deregulation or (epi) genetic alterations correlate with PCa progression. Weighted Gene Co-expression Network Analysis and other multivariate analysis techniques identified IRAK1 as one of the inflammatory signatures found to be overexpressed in over 80% of prostate adenocarcinoma (PRAD) samples. We also explored the diagnostic and prognostic potential of IRAK1 as a biomarker using Kaplan Meier Survival Analysis and AUROC Analysis. DNA methylation analysis showed that IRAK1 is hypomethylated and found to negatively correlate with its overexpression in PRAD patients. We also found some missense and truncated mutations in some patients and reported a high level of IRAK1 gene amplification in castration-resistant and neuroendocrine PCa patients.
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Date Issued: 2021
PURL: http://purl.flvc.org/fau/fd/FA00013713
Subject Headings: Prostate--Cancer, Interleukin-1 Receptor-Associated Kinases, Inflammation
Format: Document (PDF)

Title: Overcoming Multidrug Resistance in Prostate Cancer Cells Using Nanoparticle Delivery of a Two-Drug Combination.
Creator: Toluleke, O. Famuyiwa, Kumi-Diaka, James, Florida Atlantic University, Department of Biological Sciences, Charles E. Schmidt College of Science
Abstract/Description: Prostate cancer (PCa) is the second most diagnosed cancer in men. The resistance of prostate cancer to chemotherapy has been linked to the ATP Binding Cassette (ABC)-Mediated Multidrug Resistance (MDR). This study investigated the combination of 3-Bromopyruvate (3-BPA) and the anti-inflammatory molecule SC-514 in reducing MDR in prostate cancer. The compounds were incorporated into a PLGA nanoparticles to increase delivery to target cells. To investigate the effectiveness of SC-514 and/3-BPA,...
Show moreProstate cancer (PCa) is the second most diagnosed cancer in men. The resistance of prostate cancer to chemotherapy has been linked to the ATP Binding Cassette (ABC)-Mediated Multidrug Resistance (MDR). This study investigated the combination of 3-Bromopyruvate (3-BPA) and the anti-inflammatory molecule SC-514 in reducing MDR in prostate cancer. The compounds were incorporated into a PLGA nanoparticles to increase delivery to target cells. To investigate the effectiveness of SC-514 and/3-BPA, cytoxicity assays including trypan blue dye exclusion, MTT tetrazolium reduction, NBT, LDH release poly caspase detection, cell titer glow assay, and ELISA were utilized. Both immunofluorescence and multidrug resistance efflux assays were utilized to estimate the number of drug resistant cells. SC-514 was encapsulated in PLGA nanoparticles via single-emulsion method. SC-514 nanoparticles were analyzed utilizing Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM). Liquid chromatography–mass spectrometry (LC–MS) was used to measure the amount of SC- 514 released from the nanoparticle. Alternative SC-514 drug release quantification methods such as colony forming assay, wound healing assay, and transwell and migration assay were explored.
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Date Issued: 2021
PURL: http://purl.flvc.org/fau/fd/FA00013677
Subject Headings: Prostate--Cancer, Nanoparticles, Drug Delivery Systems, Multidrug resistance
Format: Document (PDF)

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