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- Title
- Genetic Engineering of Tomato Plants Expressing β-Glucuronidasethrough Agrobacterium-mediated Transformation.
- Creator
- Justs, Adriana, Kaplan, Noah, Zhang, Xing-Hai, Office of Undergraduate Research and Inquiry
- Abstract/Description
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Advancements in biotechnology have allowed us to study genetics and plant physiology by engineering transgenic plants. For our research we transformed Micro-Tom, a tomato variety developed for use in genetic research, using Agrobacterium mediated transformation. Within a time span of fourteen weeks, we inserted two distinct plasmid constructs (pCAMBIA2301 and E1492). Plants have the unique ability to regenerate their tissue and we took advantage of this ability to regenerate the transgenic...
Show moreAdvancements in biotechnology have allowed us to study genetics and plant physiology by engineering transgenic plants. For our research we transformed Micro-Tom, a tomato variety developed for use in genetic research, using Agrobacterium mediated transformation. Within a time span of fourteen weeks, we inserted two distinct plasmid constructs (pCAMBIA2301 and E1492). Plants have the unique ability to regenerate their tissue and we took advantage of this ability to regenerate the transgenic plants with antibiotic selection. Approximately one third of the explants endured the infection process and fourteen of these survived in the presence of kanamycin. By the end of the fourteenth week, eleven out of our fourteen plantlets had fully developed roots but only four survived to maturity. After verification with PCR and qPCR, we found that we generated two transgenic plants. Here we describe all the methods and techniques used to achieve these compelling results.could be the potential cause of this neurodegenerative disease, will help elucidate the role of this amino acid in ALS.
Show less - Date Issued
- 2016
- PURL
- http://purl.flvc.org/fau/fd/FA00005578
- Subject Headings
- College students --Research --United States.
- Format
- Document (PDF)
- Title
- The expression and analysis of a lysine-rich wound-response protein in tomato plants.
- Creator
- Kaplan, Noah, Zhang, Xing-Hai, Florida Atlantic University, Charles E. Schmidt College of Science, Department of Biological Sciences
- Abstract/Description
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Understanding the genetic regulation of the response to wounding and wound healing in fruiting plants is imperative to maintaining agricultural sustainability, preserving the quality of food supplies, and ensuring the economic viability of agriculture. Many genes are known to be induced by wounding, providing both structural repair and defense. The KED gene in tobacco (Nicotiana tabacum) has been shown to be induced by wounding. We have identified its homologue gene in tomato (Solanum...
Show moreUnderstanding the genetic regulation of the response to wounding and wound healing in fruiting plants is imperative to maintaining agricultural sustainability, preserving the quality of food supplies, and ensuring the economic viability of agriculture. Many genes are known to be induced by wounding, providing both structural repair and defense. The KED gene in tobacco (Nicotiana tabacum) has been shown to be induced by wounding. We have identified its homologue gene in tomato (Solanum lycopersicum) that we named SlKED. We have analyzed gene expression pattern of SlKED through tomato growth and development and in response to wounding as well as hormonal and inhibitor treatments. We found that the plant hormone ethylene played a major role in the expression of SlKED. To further identify evidence for physiological and transductional functions of KED and SlKED, the tobacco KED gene was introduced to tomato and overexpressed by the fruit tissue-active PUN1 promoter from pepper (Capsicum annuum,). The expression of this gene was compared to the expression of the native SlKED gene and other known wound response genes in both the wild-type and transgenic tomato plants. The upregulation of the native SlKED gene by wounding was significantly muted in the tobacco KED-expressing transgenic plants. The expression of other genes known to be associated with wound response transduction pathways was also altered. Our studies implicate the KED gene in defense mechanisms for mechanical stress in tomato plants.
Show less - Date Issued
- 2016
- PURL
- http://purl.flvc.org/fau/fd/FA00004773, http://purl.flvc.org/fau/fd/FA00004773
- Subject Headings
- Wound healing., Wounds and injuries--Genetic aspects., Plant gene expression., Plant genetic regulation., Nanostructured materials--Physiological effect., Biomedical engineering.
- Format
- Document (PDF)